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P62714

- PP2AB_HUMAN

UniProt

P62714 - PP2AB_HUMAN

Protein

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Gene

PPP2CB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Manganese 1By similarity
    Metal bindingi59 – 591Manganese 1By similarity
    Metal bindingi85 – 851Manganese 1By similarity
    Metal bindingi85 – 851Manganese 2By similarity
    Metal bindingi117 – 1171Manganese 2By similarity
    Active sitei118 – 1181Proton donorBy similarity
    Metal bindingi167 – 1671Manganese 2By similarity
    Metal bindingi241 – 2411Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB
    3. protein serine/threonine phosphatase activity Source: Ensembl

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: Ensembl
    2. fibroblast growth factor receptor signaling pathway Source: Reactome
    3. negative regulation of Ras protein signal transduction Source: Ensembl
    4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
    5. protein dephosphorylation Source: UniProtKB
    6. regulation of gene expression Source: Ensembl
    7. response to antibiotic Source: Ensembl
    8. response to endoplasmic reticulum stress Source: Ensembl
    9. response to hydrogen peroxide Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_11063. Degradation of beta-catenin by the destruction complex.
    REACT_11065. Beta-catenin phosphorylation cascade.
    REACT_111080. Spry regulation of FGF signaling.
    REACT_12436. ERKs are inactivated.
    REACT_12599. ERK/MAPK targets.
    REACT_1383. Glycolysis.
    REACT_150182. MASTL Facilitates Mitotic Progression.
    REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_1505. Integration of energy metabolism.
    REACT_15334. DARPP-32 events.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    REACT_19405. CTLA4 inhibitory signaling.
    REACT_200610. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_23879. Platelet sensitization by LDL.
    REACT_329. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_682. Mitotic Prometaphase.
    REACT_705. PP2A-mediated dephosphorylation of key metabolic factors.
    REACT_821. Cyclin D associated events in G1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (EC:3.1.3.16)
    Short name:
    PP2A-beta
    Gene namesi
    Name:PPP2CB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:9300. PPP2CB.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. Chromosomecentromere 1 Publication. Cytoplasmcytoskeletonspindle pole 1 Publication
    Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.

    GO - Cellular componenti

    1. chromosome, centromeric region Source: UniProtKB-SubCell
    2. cytosol Source: Reactome
    3. extracellular vesicular exosome Source: UniProt
    4. nucleus Source: UniProtKB-SubCell
    5. protein phosphatase type 2A complex Source: UniProtKB
    6. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33664.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Serine/threonine-protein phosphatase 2A catalytic subunit beta isoformPRO_0000058845Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei307 – 3071PhosphotyrosineBy similarity
    Modified residuei309 – 3091Leucine methyl ester1 Publication

    Post-translational modificationi

    Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.1 Publication
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiP62714.
    PaxDbiP62714.
    PRIDEiP62714.

    2D gel databases

    OGPiP62714.
    REPRODUCTION-2DPAGEIPI00429689.

    PTM databases

    PhosphoSiteiP62714.

    Expressioni

    Gene expression databases

    ArrayExpressiP62714.
    BgeeiP62714.
    CleanExiHS_PPP2CB.
    GenevestigatoriP62714.

    Organism-specific databases

    HPAiCAB018600.
    HPA043236.

    Interactioni

    Subunit structurei

    Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with TBCD By similarity. PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Binds PPME1. May indirectly interact with SGOL1, most probably through regulatory B56 subunits. Interacts with CTTNBP2NL.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Q13642-23EBI-1044367,EBI-8477209
    AXIN2Q9Y2T14EBI-1044367,EBI-4400025
    IGBP1P783183EBI-1044367,EBI-1055954

    Protein-protein interaction databases

    BioGridi111508. 93 interactions.
    IntActiP62714. 80 interactions.
    MINTiMINT-1348293.
    STRINGi9606.ENSP00000221138.

    Structurei

    3D structure databases

    ProteinModelPortaliP62714.
    SMRiP62714. Positions 6-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiP62714.
    KOiK04382.
    OMAiRPPDYFL.
    PhylomeDBiP62714.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62714-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDKAFTKEL DQWVEQLNEC KQLNENQVRT LCEKAKEILT KESNVQEVRC    50
    PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL 100
    VALKVRYPER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF 150
    DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW 200
    SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW 250
    CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV 300
    TRRTPDYFL 309
    Length:309
    Mass (Da):35,575
    Last modified:July 19, 2004 - v1
    Checksum:i51DA9EB0633FC191
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191E → D in AAB38020. (PubMed:2837763)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12656 mRNA. Translation: CAA31183.1.
    CR541747 mRNA. Translation: CAG46547.1.
    J03805 mRNA. Translation: AAB38020.1.
    CH471080 Genomic DNA. Translation: EAW63434.1.
    CH471080 Genomic DNA. Translation: EAW63435.1.
    CH471080 Genomic DNA. Translation: EAW63436.1.
    BC012022 mRNA. Translation: AAH12022.1.
    M60484 Genomic DNA. Translation: AAA36467.1.
    CCDSiCCDS6079.1.
    PIRiB37135. PAHU2B.
    RefSeqiNP_001009552.1. NM_001009552.1.
    UniGeneiHs.491440.

    Genome annotation databases

    EnsembliENST00000221138; ENSP00000221138; ENSG00000104695.
    GeneIDi5516.
    KEGGihsa:5516.
    UCSCiuc003xik.3. human.

    Polymorphism databases

    DMDMi50402236.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12656 mRNA. Translation: CAA31183.1 .
    CR541747 mRNA. Translation: CAG46547.1 .
    J03805 mRNA. Translation: AAB38020.1 .
    CH471080 Genomic DNA. Translation: EAW63434.1 .
    CH471080 Genomic DNA. Translation: EAW63435.1 .
    CH471080 Genomic DNA. Translation: EAW63436.1 .
    BC012022 mRNA. Translation: AAH12022.1 .
    M60484 Genomic DNA. Translation: AAA36467.1 .
    CCDSi CCDS6079.1.
    PIRi B37135. PAHU2B.
    RefSeqi NP_001009552.1. NM_001009552.1.
    UniGenei Hs.491440.

    3D structure databases

    ProteinModelPortali P62714.
    SMRi P62714. Positions 6-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111508. 93 interactions.
    IntActi P62714. 80 interactions.
    MINTi MINT-1348293.
    STRINGi 9606.ENSP00000221138.

    Chemistry

    BindingDBi P62714.
    DrugBanki DB00163. Vitamin E.

    PTM databases

    PhosphoSitei P62714.

    Polymorphism databases

    DMDMi 50402236.

    2D gel databases

    OGPi P62714.
    REPRODUCTION-2DPAGE IPI00429689.

    Proteomic databases

    MaxQBi P62714.
    PaxDbi P62714.
    PRIDEi P62714.

    Protocols and materials databases

    DNASUi 5516.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000221138 ; ENSP00000221138 ; ENSG00000104695 .
    GeneIDi 5516.
    KEGGi hsa:5516.
    UCSCi uc003xik.3. human.

    Organism-specific databases

    CTDi 5516.
    GeneCardsi GC08M030631.
    HGNCi HGNC:9300. PPP2CB.
    HPAi CAB018600.
    HPA043236.
    MIMi 176916. gene.
    neXtProti NX_P62714.
    PharmGKBi PA33664.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0639.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi P62714.
    KOi K04382.
    OMAi RPPDYFL.
    PhylomeDBi P62714.
    TreeFami TF105559.

    Enzyme and pathway databases

    Reactomei REACT_11063. Degradation of beta-catenin by the destruction complex.
    REACT_11065. Beta-catenin phosphorylation cascade.
    REACT_111080. Spry regulation of FGF signaling.
    REACT_12436. ERKs are inactivated.
    REACT_12599. ERK/MAPK targets.
    REACT_1383. Glycolysis.
    REACT_150182. MASTL Facilitates Mitotic Progression.
    REACT_150425. Resolution of Sister Chromatid Cohesion.
    REACT_150471. Separation of Sister Chromatids.
    REACT_1505. Integration of energy metabolism.
    REACT_15334. DARPP-32 events.
    REACT_1857. Cyclin A/B1 associated events during G2/M transition.
    REACT_19405. CTLA4 inhibitory signaling.
    REACT_200610. disassembly of the destruction complex and recruitment of AXIN to the membrane.
    REACT_23879. Platelet sensitization by LDL.
    REACT_329. Inhibition of replication initiation of damaged DNA by RB1/E2F1.
    REACT_682. Mitotic Prometaphase.
    REACT_705. PP2A-mediated dephosphorylation of key metabolic factors.
    REACT_821. Cyclin D associated events in G1.

    Miscellaneous databases

    ChiTaRSi PPP2CB. human.
    GeneWikii PPP2CB.
    GenomeRNAii 5516.
    NextBioi 21334.
    PROi P62714.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62714.
    Bgeei P62714.
    CleanExi HS_PPP2CB.
    Genevestigatori P62714.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit."
      Hemmings B.A., Wernet W., Mayer R., Maurer F., Hofsteenge J., Stone S.R.
      Nucleic Acids Res. 16:11366-11366(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Fibroblast.
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes."
      Arino J., Woon C.W., Brautigan D.L., Miller T.B. Jr., Johnson G.L.
      Proc. Natl. Acad. Sci. U.S.A. 85:4252-4256(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-309.
      Tissue: Liver.
    5. "Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes."
      Khew-Goodall Y., Mayer R.E., Maurer F., Stone S.R., Hemmings B.A.
      Biochemistry 30:89-97(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    7. "Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A."
      Virshup D.M., Kauffman M.G., Kelly T.J.
      EMBO J. 8:3891-3898(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 91-97; 111-114; 122-127; 137-144 AND 207-238, FUNCTION.
    8. "Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A."
      Pallas D.C., Shahrik L.K., Martin B.L., Jaspers S., Miller T.B. Jr., Brautigan D.L., Roberts T.M.
      Cell 60:167-176(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 75-89; 215-234; 270-294 AND 303-309, INTERACTION WITH VIRAL PROTEINS.
    9. "The catalytic subunit of protein phosphatase 2A is carboxyl-methylated in vivo."
      Favre B., Zolnierowicz S., Turowski P., Hemmings B.A.
      J. Biol. Chem. 269:16311-16317(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LEU-309.
    10. "A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A."
      Ogris E., Du X., Nelson K.C., Mak E.K., Yu X.X., Lane W.S., Pallas D.C.
      J. Biol. Chem. 274:14382-14391(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PME1, DEMETHYLATION.
    11. "Shugoshin collaborates with protein phosphatase 2A to protect cohesin."
      Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y.
      Nature 441:46-52(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SGOL1.
    12. "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein."
      Goudreault M., D'Ambrosio L.M., Kean M.J., Mullin M.J., Larsen B.G., Sanchez A., Chaudhry S., Chen G.I., Sicheri F., Nesvizhskii A.I., Aebersold R., Raught B., Gingras A.C.
      Mol. Cell. Proteomics 8:157-171(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CTTNBP2NL.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPP2AB_HUMAN
    AccessioniPrimary (citable) accession number: P62714
    Secondary accession number(s): D3DSV4, P11082, Q6FHK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3