P62701 (RS4X_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S4, X isoform

Alternative name(s):
SCR10
Single copy abundant mRNA protein

Gene names

Name:	RPS4X
Synonyms:	CCG2, RPS4, SCAR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Ref.9
Subcellular location	Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.9
Sequence similarities	Belongs to the ribosomal protein S4e family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome multicellular organismal development Inferred from mutant phenotype PubMed 8358435. Source: UniProtKB nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome positive regulation of cell proliferation Inferred from mutant phenotype Ref.2. Source: UniProtKB positive regulation of translation Inferred from mutant phenotype Ref.2. Source: UniProtKB translational elongation Traceable author statement. Source: Reactome translational initiation Traceable author statement. Source: Reactome translational termination Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay Ref.7. Source: UniProtKB polysome Inferred from direct assay PubMed 8139551. Source: UniProtKB
Molecular_function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from mutant phenotype PubMed 8358435. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 263	262	40S ribosomal protein S4, X isoform HAMAP-Rule MF_00485		PRO_0000130805
Regions
	Domain	42 – 104	63	S4 RNA-binding
Experimental info
	Sequence conflict	33	1	Missing in AAA36597. Ref.8
	Sequence conflict	57 – 59	3	TGD → DRR in CAA94808. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P62701 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 87200E545A8958B0	FASTA	263	29,598
	10 20 30 40 50 60 MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE 70 80 90 100 110 120 VKKICMQRFI KIDGKVRTDI TYPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK 130 140 150 160 170 180 YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI QIDLETGKIT DFIKFDTGNL 190 200 210 220 230 240 CMVTGGANLG RIGVITNRER HPGSFDVVHV KDANGNSFAT RLSNIFVIGK GNKPWISLPR 250 260 GKGIRLTIAE ERDKRLAAKQ SSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologous ribosomal protein genes on the human X and Y chromosomes: escape from X inactivation and possible implications for Turner syndrome." Fisher E.M.C., Beer-Romero P., Brown L.G., Ridley A., McNeil J.A., Lawrence J.B., Willard H.F., Bieber F.R., Page D.C. Cell 63:1205-1218(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of the human gene, CCG2, that complements the BHK-derived temperature-sensitive cell cycle mutant tsBN63: identity of CCG2 with the human X chromosomal SCAR/RPS4X gene." Watanabe M., Furuno N., Goebl M., Go M., Miyauchi K., Sekiguchi T., Basilico C., Nishimoto T. J. Cell Sci. 100:35-43(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Zuo L., Baybayan P., Kuang W.-J., Brown L., Page D., Chen E. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[6]	"Characterization of different mRNA types expressed in human brain." Dmitrenko V.V., Garifulin O.M., Kavsan V.M. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-59. Tissue: Brain.
[7]	"Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K., Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A. Eur. J. Biochem. 239:144-149(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Tissue: Placenta.
[8]	"Isolation of an abundantly expressed sequence from the human X chromosome by differential screening." Wiles M.V., Alexander C.M., Goodfellow P.N. Somat. Cell Mol. Genet. 14:31-39(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-263.
[9]	"Molecular composition of IMP1 ribonucleoprotein granules." Joeson L., Vikesaa J., Krogh A., Nielsen L.K., Hansen T., Borup R., Johnsen A.H., Christiansen J., Nielsen F.C. Mol. Cell. Proteomics 6:798-811(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MRNP GRANULE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58458 mRNA. Translation: AAA63255.1. AF041428 Genomic DNA. Translation: AAB96968.1. CR456735 mRNA. Translation: CAG33016.1. BC000472 mRNA. Translation: AAH00472.1. BC071662 mRNA. Translation: AAH71662.1. BC100903 mRNA. Translation: AAI00904.1. BC100904 mRNA. Translation: AAI00905.1. Z70767 mRNA. Translation: CAA94808.1. M22146 mRNA. Translation: AAA36597.1.
IPI	IPI00217030.
PIR	R3HU4X. B36338.
RefSeq	NP_000998.1. NM_001007.4.
UniGene	Hs.118076.
3D structure databases
ProteinModelPortal	P62701.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62701. 21 interactions.
MINT	MINT-1159032.
STRING	9606.ENSP00000362744.
PTM databases
PhosphoSite	P62701.
Polymorphism databases
DMDM	50403628.
2D gel databases
SWISS-2DPAGE	P62701.
Proteomic databases
PaxDb	P62701.
PeptideAtlas	P62701.
PRIDE	P62701.
Protocols and materials databases
DNASU	6191.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000316084; ENSP00000362744; ENSG00000198034.
GeneID	6191.
KEGG	hsa:6191.
UCSC	uc004ear.3. human.
Organism-specific databases
CTD	6191.
GeneCards	GC0XM071475.
HGNC	HGNC:10424. RPS4X.
HPA	HPA000857.
MIM	312760. gene.
neXtProt	NX_P62701.
PharmGKB	PA34839.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1471.
HOVERGEN	HBG000935.
InParanoid	P62701.
KO	K02987.
OMA	GHIQLNL.
OrthoDB	EOG4QC161.
PhylomeDB	P62701.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P62701.
Bgee	P62701.
CleanEx	HS_RPS4X.
Genevestigator	P62701.
GermOnline	ENSG00000198034. Homo sapiens.
Family and domain databases
HAMAP	MF_00485. Ribosomal_S4e.
InterPro	IPR005824. KOW. IPR000876. Ribosomal_S4e. IPR013845. Ribosomal_S4e_central. IPR013843. Ribosomal_S4e_N. IPR018199. Ribosomal_S4e_N_CS. IPR002942. S4_RNA-bd. [Graphical view]
PANTHER	PTHR11581. PTHR11581. 1 hit.
Pfam	PF00467. KOW. 1 hit. PF00900. Ribosomal_S4e. 1 hit. PF08071. RS4NT. 1 hit. PF01479. S4. 1 hit. [Graphical view]
PIRSF	PIRSF002116. Ribosomal_S4. 1 hit.
ProDom	PD002667. Ribosomal_S4e_central. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00363. S4. 1 hit. [Graphical view]
PROSITE	PS00528. RIBOSOMAL_S4E. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPS4X. human.
GenomeRNAi	6191.
NextBio	24041.
PMAP-CutDB	P62701.
SOURCE	Search...

Entry information

Entry name

RS4X_HUMAN

Accession

Primary (citable) accession number: P62701
Secondary accession number(s): P12631 , P12750, P27576, P55831, Q14727, Q6IPY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.