P62639 (CHEB2_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	GSU1145

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000157997
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	164 – 354	191	CheB-type methylesterase
Sites
	Active site	176	1	By similarity
	Active site	202	1	By similarity
	Active site	298	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62639 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 03B9AD2747D872D3	FASTA	354	38,277
	10 20 30 40 50 60 MKKIKVLIVD DSAVVRQTMA DILASDPQIE VMATAADPFI AAERMKSDVP DVITLDVEMP 70 80 90 100 110 120 RMDGITFLQK IMSQHPIPVV MCSTLTENGS ETAMKALEYG AVEIIQKPKL GTKQFLEESR 130 140 150 160 170 180 VRICDAIKAA SQARLRKIPA RPHAVAPKLS ADVILEKPAS RAMIQTTERV VVVGASTGGT 190 200 210 220 230 240 EALRVFLESF PADCPPIVIV QHMPEGFTRA FAQRLDGICR ISVKEAADND SVIRGRALIA 250 260 270 280 290 300 PGNRHMLLKR SGARYYVEIK DGPLVSRHRP SVDVLFRSAA RYAGKNAVGV IMTGMGDDGA 310 320 330 340 350 SGMREMKDAG AMTIAQDEAS CVVFGMPNEA IKRGGTVKVL PLESIAADVI RHCS

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR34521.1.
RefSeq	NP_952198.1. NC_002939.4.
3D structure databases
ProteinModelPortal	P62639.
SMR	P62639. Positions 1-353.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2685549.
GenomeReviews	Gene locus GSU1145 in contig AE017180_GR.
KEGG	gsu:GSU1145.
NMPDR	fig|243231.1.peg.1136.
PATRIC	22025062. VBIGeoSul17553_1141.
TIGR	GSU1145.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	P62639.
ProtClustDB	CLSK828211.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_1145-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_GEOSL

Accession

Primary (citable) accession number: P62639
Secondary accession number(s): Q74E19

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program