P62638 (CHEB1_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	GSU0293

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000157996
Regions
	Domain	10 – 127	118	Response regulatory
	Domain	163 – 362	200	CheB-type methylesterase
Sites
	Active site	181	1	By similarity
	Active site	208	1	By similarity
	Active site	306	1	By similarity
Amino acid modifications
	Modified residue	61	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62638 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 2ED13472A9ACA970	FASTA	362	38,340
	10 20 30 40 50 60 MLQNQTRKLR VLVVDDSSFM RMVIRSVLEK DPAIEVVGIA VDGMEGVEKA LALKPDLITM 70 80 90 100 110 120 DIEMPRLDGI SALKQVMAKC PTRVLMVSTL TCEGAKATFD ALDAGAIDYI PKNVTDSADA 130 140 150 160 170 180 QRVFREELLR KVKGAASSIF GRPMTTSAPR AIIAPPRQVQ PAPRPSQALA GKFHYVGIGA 190 200 210 220 230 240 STGGPVALQE VLGRIPGNYP HGIVVAIHMP KAFTGPYAER LNSKCSLQIK EANDGDIIQP 250 260 270 280 290 300 GVVLVAPGGR HMALARQGNS IVVRTLSTAE CPQYIYIPSV DHMMTTLADA TNGSALGVIL 310 320 330 340 350 360 TGMGSDGFKG MKHLKSKGGI TIVQDEATST IYGMPRACIE GGVADTVLPL TQIGSEIARL GG

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR33626.1.
RefSeq	NP_951353.1. NC_002939.4.
3D structure databases
ProteinModelPortal	P62638.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2686644.
GenomeReviews	Gene locus GSU0293 in contig AE017180_GR.
KEGG	gsu:GSU0293.
NMPDR	fig|243231.1.peg.291.
PATRIC	22023328. VBIGeoSul17553_0290.
TIGR	GSU0293.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	SAGSTIW.
PhylomeDB	P62638.
ProtClustDB	CLSK827727.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_0293-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_GEOSL

Accession

Primary (citable) accession number: P62638
Secondary accession number(s): Q74GF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program