Reviewed, UniProtKB/Swiss-Prot P62637 (CHEB2_DESVH)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	DVU_2078

Organism

Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) [Complete proteome] [HAMAP]

Taxonomic identifier

882 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000157991
Regions
	Domain	3 – 120	118	Response regulatory
	Domain	175 – 367	193	CheB-type methylesterase
Sites
	Active site	187	1	By similarity
	Active site	214	1	By similarity
	Active site	310	1	By similarity
Amino acid modifications
	Modified residue	54	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62637-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: FE21D478861A22E2	FASTA	367	39,075
	10 20 30 40 50 60 MISVVVVDDS AFMRKALSTM LEKDPEIRVV ATARDGEEGL QVIRQHNPDV VTLDIEMPRM 70 80 90 100 110 120 DGLTTLRHIM MEMPRPVLMV SSLTTEGAEA TLKALELGAV DFIPKQLSKV SLDIVRIEND 130 140 150 160 170 180 LREKVKEVSK RRMLRTPRPV RPAPTASAPA QTAQVASAAP ATAPSRPAMP ATRASRPVRD 190 200 210 220 230 240 VVAIGVSTGG PPAVQKVLSQ LPADFPASIL IAQHMPAAFT GPFAKRLDGV CAISVKEAES 250 260 270 280 290 300 GEKLKPGTAY IAPGGKHLRV EQRVSHMEVV VTTDPADALY KPSANVLMES VGQSMGRRAL 310 320 330 340 350 360 GVILTGMGSD GMEGMKVLKQ KGGRSIAQSD ATCVVYGMPK AIVDAGLADE IVDIDDMAAA IMNGLYK

References

[1]

"The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough." Heidelberg J.F., Seshadri R., Haveman S.A., Hemme C.L., Paulsen I.T., Kolonay J.F., Eisen J.A., Ward N.L., Methe B.A., Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Fouts D.E. , Haft D.H., Selengut J., Peterson J.D., Davidsen T.M., Zafar N., Zhou L., Radune D., Dimitrov G., Hance M., Tran K., Khouri H.M., Gill J., Utterback T.R., Feldblyum T.V., Wall J.D., Voordouw G., Fraser C.M. Nat. Biotechnol. 22:554-559(2004) [PubMed: 15077118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017285 Genomic DNA. Translation: AAS96551.1.
RefSeq	YP_011291.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P62637.
Genome annotation databases
GeneID	2793455.
GenomeReviews	Gene locus DVU_2078 in contig AE017285_GR.
KEGG	dvu:DVU2078.
NMPDR	fig|882.1.peg.2066.
TIGR	DVU_2078.
Phylogenomic databases
HOGENOM	P62637.
OMA	VPIFVVQ.
Enzyme and pathway databases
BioCyc	DVUL882:DVU_2078-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_DESVH

Accession

Primary (citable) accession number: P62637

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)