P62636 (CHEB1_DESVH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	DVU_1596

Organism

Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303)

Taxonomic identifier

882 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfovibrionales › Desulfovibrionaceae › Desulfovibrio

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000157990
Regions
	Domain	6 – 123	118	Response regulatory
	Domain	165 – 357	193	CheB-type methylesterase
Sites
	Active site	177	1	By similarity
	Active site	203	1	By similarity
	Active site	299	1	By similarity
Amino acid modifications
	Modified residue	57	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62636 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 44DD7F9E15B22AD5	FASTA	357	38,232
	10 20 30 40 50 60 MARPCRVLIV DDSALVRQTL ADLLSADPGI EVVGTASDPF VAAKRMEAVV PDVILLDIEM 70 80 90 100 110 120 PRMDGLTFLR KIMTQHPLPV VICSSVTEQG AEATFKAMEY GAVEVITKPR LGTKRFLEES 130 140 150 160 170 180 SIRICDAVKA AARARLHRLG HRTRDVAPKL TADAMLPGPT NFTRVQTTEK VVVVGASTGG 190 200 210 220 230 240 TEALQVFLEA MPPDAPPIAI VQHMPEHFTG AFSRRLDGIC RIRVREAEDG DIMARGQALI 250 260 270 280 290 300 APGSSHMLLK RNGSRYTVEV KDGPLVRRHR PSVDVLFRSA ARYAGANAVA AILTGMGDDG 310 320 330 340 350 AAGMKELHDM GAHTIAQDEA TCIVFGMPHE AIRLGGVDRV LPLDAIAPAV LKACAGS

References

[1]

"The genome sequence of the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough." Heidelberg J.F., Seshadri R., Haveman S.A., Hemme C.L., Paulsen I.T., Kolonay J.F., Eisen J.A., Ward N.L., Methe B.A., Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Fouts D.E. , Haft D.H., Selengut J., Peterson J.D., Davidsen T.M., Zafar N., Zhou L., Radune D., Dimitrov G., Hance M., Tran K., Khouri H.M., Gill J., Utterback T.R., Feldblyum T.V., Wall J.D., Voordouw G., Fraser C.M. Nat. Biotechnol. 22:554-559(2004) [PubMed: 15077118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hildenborough / ATCC 29579 / NCIMB 8303.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017285 Genomic DNA. Translation: AAS96074.1.
RefSeq	YP_010815.1. NC_002937.3.
3D structure databases
ProteinModelPortal	P62636.
ModBase	Search...
Protein-protein interaction databases
STRING	P62636.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2795951.
GenomeReviews	Gene locus DVU_1596 in contig AE017285_GR.
KEGG	dvu:DVU1596.
PATRIC	32062988. VBIDesVul119526_1471.
TIGR	DVU_1596.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	P62636.
ProtClustDB	CLSK830253.
Enzyme and pathway databases
BioCyc	DVUL882:DVU_1596-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_DESVH

Accession

Primary (citable) accession number: P62636

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program