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Protein

Elongation factor 1-alpha 1

Gene

Eef1a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPBy similarity8
Nucleotide bindingi91 – 95GTPBy similarity5
Nucleotide bindingi153 – 156GTPBy similarity4

GO - Molecular functioni

  • drug binding Source: RGD
  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • mRNA binding Source: RGD
  • translation elongation factor activity Source: UniProtKB-KW

GO - Biological processi

  • aging Source: RGD
  • cellular response to drug Source: RGD
  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • cellular response to nerve growth factor stimulus Source: RGD
  • negative regulation of reactive oxygen species biosynthetic process Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • protein homooligomerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-alpha 1
Short name:
EF-1-alpha-1
Alternative name(s):
Elongation factor Tu
Short name:
EF-Tu
Eukaryotic elongation factor 1 A-1
Short name:
eEF1A-1
Gene namesi
Name:Eef1a1
Synonyms:Eef1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi67387. Eef1a1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Nucleusnucleolus

  • Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery. Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: RGD
  • nucleolus Source: UniProtKB
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000908901 – 462Elongation factor 1-alpha 1Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36N6,N6,N6-trimethyllysineBy similarity1
Modified residuei55N6,N6-dimethyllysineBy similarity1
Modified residuei79N6,N6,N6-trimethyllysineBy similarity1
Modified residuei165N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei165N6-acetyllysine; alternateBy similarity1
Modified residuei172N6-acetyllysineBy similarity1
Modified residuei273N6-acetyllysineBy similarity1
Modified residuei300Phosphoserine; by TGFBR1By similarity1
Modified residuei3015-glutamyl glycerylphosphorylethanolamineBy similarity1
Modified residuei318N6,N6,N6-trimethyllysineBy similarity1
Modified residuei3745-glutamyl glycerylphosphorylethanolamineBy similarity1
Modified residuei392N6-acetyllysine; alternateBy similarity1
Modified residuei392N6-succinyllysine; alternateBy similarity1
Modified residuei432Phosphothreonine; by PASKBy similarity1
Modified residuei439N6-acetyllysineBy similarity1

Post-translational modificationi

ISGylated.By similarity
Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency (By similarity).By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62630.
PRIDEiP62630.

2D gel databases

World-2DPAGE0004:P62630.

PTM databases

iPTMnetiP62630.
PhosphoSitePlusiP62630.
SwissPalmiP62630.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009439.
GenevisibleiP62630. RN.

Interactioni

Subunit structurei

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS (By similarity). May interact with ERGIC2 (By similarity). Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi251204. 5 interactors.
IntActiP62630. 5 interactors.
MINTiMINT-87282.
STRINGi10116.ENSRNOP00000013608.

Structurei

3D structure databases

ProteinModelPortaliP62630.
SMRiP62630.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 242tr-type GAdd BLAST238

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1By similarity8
Regioni70 – 74G2By similarity5
Regioni91 – 94G3By similarity4
Regioni153 – 156G4By similarity4
Regioni194 – 196G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiP62630.
KOiK03231.
OMAiFEQISHE.
OrthoDBiEOG091G05LW.
PhylomeDBiP62630.
TreeFamiTF300304.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62630-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Length:462
Mass (Da):50,114
Last modified:July 19, 2004 - v1
Checksum:i71072871DE7405DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187T → H in AAA91895 (PubMed:7945283).Curated1
Sequence conflicti360 – 365VLDCHT → ILDDYM in AAA41105 (PubMed:1730661).Curated6
Sequence conflicti374E → R in AAA41105 (PubMed:1730661).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63561 mRNA. Translation: CAA45122.1.
X61043 mRNA. Translation: CAA43378.1.
L10339 mRNA. Translation: AAA91895.1.
BC063162 mRNA. Translation: AAH63162.1.
BC072542 mRNA. Translation: AAH72542.1.
BC091297 mRNA. Translation: AAH91297.1.
BC111707 mRNA. Translation: AAI11708.1.
BC128723 mRNA. Translation: AAI28724.1.
M81088 mRNA. Translation: AAA41105.1.
PIRiS21055.
RefSeqiNP_787032.1. NM_175838.1.
UniGeneiRn.144660.
Rn.965.

Genome annotation databases

EnsembliENSRNOT00000013608; ENSRNOP00000013608; ENSRNOG00000009439.
GeneIDi171361.
KEGGirno:171361.
UCSCiRGD:67387. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63561 mRNA. Translation: CAA45122.1.
X61043 mRNA. Translation: CAA43378.1.
L10339 mRNA. Translation: AAA91895.1.
BC063162 mRNA. Translation: AAH63162.1.
BC072542 mRNA. Translation: AAH72542.1.
BC091297 mRNA. Translation: AAH91297.1.
BC111707 mRNA. Translation: AAI11708.1.
BC128723 mRNA. Translation: AAI28724.1.
M81088 mRNA. Translation: AAA41105.1.
PIRiS21055.
RefSeqiNP_787032.1. NM_175838.1.
UniGeneiRn.144660.
Rn.965.

3D structure databases

ProteinModelPortaliP62630.
SMRiP62630.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251204. 5 interactors.
IntActiP62630. 5 interactors.
MINTiMINT-87282.
STRINGi10116.ENSRNOP00000013608.

PTM databases

iPTMnetiP62630.
PhosphoSitePlusiP62630.
SwissPalmiP62630.

2D gel databases

World-2DPAGE0004:P62630.

Proteomic databases

PaxDbiP62630.
PRIDEiP62630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013608; ENSRNOP00000013608; ENSRNOG00000009439.
GeneIDi171361.
KEGGirno:171361.
UCSCiRGD:67387. rat.

Organism-specific databases

CTDi1915.
RGDi67387. Eef1a1.

Phylogenomic databases

eggNOGiKOG0052. Eukaryota.
COG5256. LUCA.
GeneTreeiENSGT00670000097815.
HOGENOMiHOG000229291.
HOVERGENiHBG000179.
InParanoidiP62630.
KOiK03231.
OMAiFEQISHE.
OrthoDBiEOG091G05LW.
PhylomeDBiP62630.
TreeFamiTF300304.

Miscellaneous databases

PROiP62630.

Gene expression databases

BgeeiENSRNOG00000009439.
GenevisibleiP62630. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_A. EF_Tu_A. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004539. Transl_elong_EF1A_euk/arc.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00483. EF-1_alpha. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1A1_RAT
AccessioniPrimary (citable) accession number: P62630
Secondary accession number(s): P20001
, Q5BJW8, Q64718, Q78ED4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.