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Protein

Dynein light chain roadblock-type 1

Gene

Dynlrb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.

GO - Molecular functioni

  • dynein intermediate chain binding Source: GO_Central
  • identical protein binding Source: UniProtKB
  • motor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain roadblock-type 1
Alternative name(s):
Dynein light chain 2A, cytoplasmic
Gene namesi
Name:Dynlrb1
Synonyms:Dncl2a, Dnlc2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914318. Dynlrb1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cytoplasm Source: UniProtKB
  • cytoplasmic dynein complex Source: UniProtKB
  • membrane Source: MGI
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002209562 – 96Dynein light chain roadblock-type 1Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP62627.
MaxQBiP62627.
PaxDbiP62627.
PRIDEiP62627.
TopDownProteomicsiP62627.

2D gel databases

UCD-2DPAGEP62627.

PTM databases

iPTMnetiP62627.
PhosphoSitePlusiP62627.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047459.
CleanExiMM_DYNLRB1.
ExpressionAtlasiP62627. baseline and differential.
GenevisibleiP62627. MM.

Interactioni

Subunit structurei

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1I1 and DYNC1I2. Self-associates. Interacts with DYNLRB2. Interacts with RAB6A; the interaction is direct. Interacts with RAB6B (GDP-bound) (By similarity).By similarity

GO - Molecular functioni

  • dynein intermediate chain binding Source: GO_Central
  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi211916. 15 interactors.
IntActiP62627. 15 interactors.
MINTiMINT-4093134.
STRINGi10090.ENSMUSP00000105304.

Structurei

Secondary structure

196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 13Combined sources12
Beta strandi17 – 24Combined sources8
Turni25 – 27Combined sources3
Beta strandi28 – 34Combined sources7
Helixi36 – 60Combined sources25
Beta strandi66 – 75Combined sources10
Beta strandi77 – 82Combined sources6
Beta strandi84 – 92Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y4ONMR-A/B2-96[»]
ProteinModelPortaliP62627.
SMRiP62627.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62627.

Family & Domainsi

Sequence similaritiesi

Belongs to the GAMAD family.Curated

Phylogenomic databases

eggNOGiKOG4115. Eukaryota.
ENOG4111NDV. LUCA.
GeneTreeiENSGT00390000011067.
HOGENOMiHOG000232033.
HOVERGENiHBG058598.
InParanoidiP62627.
KOiK10419.
PhylomeDBiP62627.
TreeFamiTF315165.

Family and domain databases

InterProiIPR016561. DYNLRB1/2.
IPR004942. Roadblock/LAMTOR2_dom.
[Graphical view]
PfamiPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFiPIRSF009998. DLC7. 1 hit.
SMARTiSM00960. Robl_LC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYANLMHNFI
60 70 80 90
LKARSTVREI DPQNDLTFLR IRSKKNEIMV APDKDYFLIV IQNPTE
Length:96
Mass (Da):10,990
Last modified:January 23, 2007 - v3
Checksum:i666734B413AFE7D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3E → D in BAB25768 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002359 mRNA. Translation: BAB22038.1.
AK003435 mRNA. Translation: BAB22788.1.
AK008595 mRNA. Translation: BAB25768.1.
AK166685 mRNA. Translation: BAE38944.1.
AL929588 Genomic DNA. Translation: CAM27181.1.
BC099423 mRNA. Translation: AAH99423.1.
BC125610 mRNA. Translation: AAI25611.1.
BC125648 mRNA. Translation: AAI25649.1.
CCDSiCCDS38294.1.
RefSeqiNP_080223.2. NM_025947.3.
UniGeneiMm.23693.

Genome annotation databases

EnsembliENSMUST00000109682; ENSMUSP00000105304; ENSMUSG00000047459.
GeneIDi67068.
KEGGimmu:67068.
UCSCiuc008nkg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002359 mRNA. Translation: BAB22038.1.
AK003435 mRNA. Translation: BAB22788.1.
AK008595 mRNA. Translation: BAB25768.1.
AK166685 mRNA. Translation: BAE38944.1.
AL929588 Genomic DNA. Translation: CAM27181.1.
BC099423 mRNA. Translation: AAH99423.1.
BC125610 mRNA. Translation: AAI25611.1.
BC125648 mRNA. Translation: AAI25649.1.
CCDSiCCDS38294.1.
RefSeqiNP_080223.2. NM_025947.3.
UniGeneiMm.23693.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y4ONMR-A/B2-96[»]
ProteinModelPortaliP62627.
SMRiP62627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211916. 15 interactors.
IntActiP62627. 15 interactors.
MINTiMINT-4093134.
STRINGi10090.ENSMUSP00000105304.

PTM databases

iPTMnetiP62627.
PhosphoSitePlusiP62627.

2D gel databases

UCD-2DPAGEP62627.

Proteomic databases

EPDiP62627.
MaxQBiP62627.
PaxDbiP62627.
PRIDEiP62627.
TopDownProteomicsiP62627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109682; ENSMUSP00000105304; ENSMUSG00000047459.
GeneIDi67068.
KEGGimmu:67068.
UCSCiuc008nkg.2. mouse.

Organism-specific databases

CTDi83658.
MGIiMGI:1914318. Dynlrb1.

Phylogenomic databases

eggNOGiKOG4115. Eukaryota.
ENOG4111NDV. LUCA.
GeneTreeiENSGT00390000011067.
HOGENOMiHOG000232033.
HOVERGENiHBG058598.
InParanoidiP62627.
KOiK10419.
PhylomeDBiP62627.
TreeFamiTF315165.

Enzyme and pathway databases

ReactomeiR-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiDynlrb1. mouse.
EvolutionaryTraceiP62627.
PROiP62627.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047459.
CleanExiMM_DYNLRB1.
ExpressionAtlasiP62627. baseline and differential.
GenevisibleiP62627. MM.

Family and domain databases

InterProiIPR016561. DYNLRB1/2.
IPR004942. Roadblock/LAMTOR2_dom.
[Graphical view]
PfamiPF03259. Robl_LC7. 1 hit.
[Graphical view]
PIRSFiPIRSF009998. DLC7. 1 hit.
SMARTiSM00960. Robl_LC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLRB1_MOUSE
AccessioniPrimary (citable) accession number: P62627
Secondary accession number(s): A2AVR8
, O88567, Q4FZJ8, Q9D812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.