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Protein

4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)

Gene

ispG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate, using flavodoxin as the reducing agent.UniRule annotation2 Publications

Catalytic activityi

(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin.UniRule annotation3 Publications

Cofactori

[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 1 [4Fe-4S] cluster.UniRule annotation2 Publications

pH dependencei

Optimum pH is 9.1 Publication

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 5 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation2 Publications
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi270Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi273Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi305Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi312Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

  • 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron ion binding Source: InterPro
  • metal ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10370-MONOMER.
ECOL316407:JW2499-MONOMER.
MetaCyc:EG10370-MONOMER.
BRENDAi1.17.7.1. 2026.
UniPathwayiUPA00056; UER00096.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)UniRule annotation (EC:1.17.7.3UniRule annotation3 Publications)
Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthaseUniRule annotation
Protein GcpE
Short name:
Protein E
Gene namesi
Name:ispGUniRule annotation
Synonyms:gcpE
Ordered Locus Names:b2515, JW2499
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10370. ispG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi322G → D: Loss of activity. 1 Publication1
Mutagenesisi328G → D: Loss of activity. 1 Publication1
Mutagenesisi335R → C: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001905721 – 3724-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)Add BLAST372

Proteomic databases

EPDiP62620.
PaxDbiP62620.
PRIDEiP62620.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ecpBP771882EBI-550338,EBI-1113651

Protein-protein interaction databases

BioGridi4260590. 337 interactors.
DIPiDIP-35832N.
IntActiP62620. 10 interactors.
STRINGi511145.b2515.

Structurei

3D structure databases

ProteinModelPortaliP62620.
SMRiP62620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4E. Bacteria.
COG0821. LUCA.
HOGENOMiHOG000261019.
InParanoidiP62620.
KOiK03526.
OMAiHNGMKIA.
PhylomeDBiP62620.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011005. Dihydropteroate_synth-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.

Sequencei

Sequence statusi: Complete.

P62620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNQAPIQRR KSTRIYVGNV PIGDGAPIAV QSMTNTRTTD VEATVNQIKA
60 70 80 90 100
LERVGADIVR VSVPTMDAAE AFKLIKQQVN VPLVADIHFD YRIALKVAEY
110 120 130 140 150
GVDCLRINPG NIGNEERIRM VVDCARDKNI PIRIGVNAGS LEKDLQEKYG
160 170 180 190 200
EPTPQALLES AMRHVDHLDR LNFDQFKVSV KASDVFLAVE SYRLLAKQID
210 220 230 240 250
QPLHLGITEA GGARSGAVKS AIGLGLLLSE GIGDTLRVSL AADPVEEIKV
260 270 280 290 300
GFDILKSLRI RSRGINFIAC PTCSRQEFDV IGTVNALEQR LEDIITPMDV
310 320 330 340 350
SIIGCVVNGP GEALVSTLGV TGGNKKSGLY EDGVRKDRLD NNDMIDQLEA
360 370
RIRAKASQLD EARRIDVQQV EK
Length:372
Mass (Da):40,684
Last modified:July 19, 2004 - v1
Checksum:i3666FD83D5CD4F9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64451 Genomic DNA. Translation: CAA45783.1.
AY033515 Genomic DNA. Translation: AAK53460.1.
U00096 Genomic DNA. Translation: AAC75568.1.
AP009048 Genomic DNA. Translation: BAA16402.1.
M11843 Genomic DNA. No translation available.
PIRiS23058.
RefSeqiNP_417010.1. NC_000913.3.
WP_000551807.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75568; AAC75568; b2515.
BAA16402; BAA16402; BAA16402.
GeneIDi946991.
KEGGiecj:JW2499.
eco:b2515.
PATRICi32120423. VBIEscCol129921_2614.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64451 Genomic DNA. Translation: CAA45783.1.
AY033515 Genomic DNA. Translation: AAK53460.1.
U00096 Genomic DNA. Translation: AAC75568.1.
AP009048 Genomic DNA. Translation: BAA16402.1.
M11843 Genomic DNA. No translation available.
PIRiS23058.
RefSeqiNP_417010.1. NC_000913.3.
WP_000551807.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP62620.
SMRiP62620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260590. 337 interactors.
DIPiDIP-35832N.
IntActiP62620. 10 interactors.
STRINGi511145.b2515.

Proteomic databases

EPDiP62620.
PaxDbiP62620.
PRIDEiP62620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75568; AAC75568; b2515.
BAA16402; BAA16402; BAA16402.
GeneIDi946991.
KEGGiecj:JW2499.
eco:b2515.
PATRICi32120423. VBIEscCol129921_2614.

Organism-specific databases

EchoBASEiEB0365.
EcoGeneiEG10370. ispG.

Phylogenomic databases

eggNOGiENOG4105C4E. Bacteria.
COG0821. LUCA.
HOGENOMiHOG000261019.
InParanoidiP62620.
KOiK03526.
OMAiHNGMKIA.
PhylomeDBiP62620.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00096.
BioCyciEcoCyc:EG10370-MONOMER.
ECOL316407:JW2499-MONOMER.
MetaCyc:EG10370-MONOMER.
BRENDAi1.17.7.1. 2026.

Miscellaneous databases

PROiP62620.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00159. IspG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011005. Dihydropteroate_synth-like.
IPR016425. IspG_bac.
IPR004588. IspG_bac-typ.
[Graphical view]
PfamiPF04551. GcpE. 1 hit.
[Graphical view]
PIRSFiPIRSF004640. IspG. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR00612. ispG_gcpE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiISPG_ECOLI
AccessioniPrimary (citable) accession number: P62620
Secondary accession number(s): P27433, P76984, P76985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.