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Protein

Cytoplasmic trehalase

Gene

treF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity.

Catalytic activityi

Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose.UniRule annotation

Enzyme regulationi

Activity decreases with increasing salt concentrations.

pH dependencei

Optimum pH is 6.0.

Pathwayi: trehalose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-glucose from alpha,alpha-trehalose.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Cytoplasmic trehalase (treF)
This subpathway is part of the pathway trehalose degradation, which is itself part of Glycan degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucose from alpha,alpha-trehalose, the pathway trehalose degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei168SubstrateUniRule annotation1
Binding sitei212SubstrateUniRule annotation1
Binding sitei324Substrate; via carbonyl oxygenUniRule annotation1
Active sitei326Proton donor/acceptorUniRule annotation1
Active sitei509Proton donor/acceptorUniRule annotation1
Binding sitei525SubstrateUniRule annotation1

GO - Molecular functioni

  • alpha,alpha-trehalase activity Source: EcoCyc

GO - Biological processi

  • cellular hyperosmotic response Source: EcoCyc
  • trehalose catabolic process Source: EcoCyc

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:TREHALACYTO-MONOMER
MetaCyc:TREHALACYTO-MONOMER
UniPathwayiUPA00300; UER00535

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic trehalaseUniRule annotation (EC:3.2.1.28UniRule annotation)
Alternative name(s):
Alpha,alpha-trehalaseUniRule annotation
Alpha,alpha-trehalose glucohydrolaseUniRule annotation
Gene namesi
Name:treFUniRule annotation
Ordered Locus Names:b3519, JW3487
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG12245 treF

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001737861 – 549Cytoplasmic trehalaseAdd BLAST549

Proteomic databases

PaxDbiP62601
PRIDEiP62601

Expressioni

Inductioni

Weakly induced by high osmolarity but not by trehalose. Expression is partially dependent on RpoS.

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi4260775, 13 interactors
IntActiP62601, 2 interactors
STRINGi316385.ECDH10B_3696

Structurei

3D structure databases

ProteinModelPortaliP62601
SMRiP62601
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 176Substrate bindingUniRule annotation2
Regioni221 – 223Substrate bindingUniRule annotation3
Regioni292 – 294Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 37 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E53 Bacteria
COG1626 LUCA
HOGENOMiHOG000215464
InParanoidiP62601
KOiK01194
OMAiDAPFGWA
PhylomeDBiP62601

Family and domain databases

Gene3Di1.50.10.10, 1 hit
HAMAPiMF_01059 Cyt_trehalase, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR023715 Cyt_trehalase
IPR001661 Glyco_hydro_37
IPR018232 Glyco_hydro_37_CS
PANTHERiPTHR23403 PTHR23403, 1 hit
PfamiView protein in Pfam
PF01204 Trehalase, 1 hit
PRINTSiPR00744 GLHYDRLASE37
SUPFAMiSSF48208 SSF48208, 1 hit
PROSITEiView protein in PROSITE
PS00927 TREHALASE_1, 1 hit
PS00928 TREHALASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P62601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS
60 70 80 90 100
DALTPADRYL ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH
110 120 130 140 150
RDFDLRKFVE NHFWLPEVYS SEYVSDPQNS LKEHIDQLWP VLTREPQDHI
160 170 180 190 200
PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT MLGLAESGRE DLLKCMADNF
210 220 230 240 250
AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR GARRYLDHLK
260 270 280 290 300
MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV
310 320 330 340 350
ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID
360 370 380 390 400
LNAFLFKLES AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI
410 420 430 440 450
YRDYDWRREQ LALFSAAAIV PLYVGMANHE QADRLANAVR SRLLTPGGIL
460 470 480 490 500
ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY GDDLLGDEIA RSWLKTVNQF
510 520 530 540
YLEQHKLIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR RLIGLYGEP
Length:549
Mass (Da):63,697
Last modified:July 19, 2004 - v1
Checksum:i543B82A7FAD4CB9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA Translation: AAB18495.1
U00096 Genomic DNA Translation: AAC76544.1
AP009048 Genomic DNA Translation: BAE77775.1
PIRiS47739
RefSeqiNP_417976.1, NC_000913.3
WP_000934216.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76544; AAC76544; b3519
BAE77775; BAE77775; BAE77775
GeneIDi948037
KEGGiecj:JW3487
eco:b3519
PATRICifig|1411691.4.peg.3199

Entry informationi

Entry nameiTREF_ECOLI
AccessioniPrimary (citable) accession number: P62601
Secondary accession number(s): P37196, Q2M7I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

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