P62601 (TREF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytoplasmic trehalase
EC=3.2.1.28

Alternative name(s):
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Gene names

Name:	treF
Ordered Locus Names:	b3519, JW3487

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity. HAMAP-Rule MF_01059
Catalytic activity	Alpha,alpha-trehalose + H2O = beta-D-glucose + alpha-D-glucose. HAMAP-Rule MF_01059
Enzyme regulation	Activity decreases with increasing salt concentrations. HAMAP-Rule MF_01059
Pathway	Glycan degradation; trehalose degradation; D-glucose from alpha,alpha-trehalose: step 1/1. HAMAP-Rule MF_01059
Subunit structure	Monomer Probable.
Subcellular location	Cytoplasm Ref.5.
Induction	Weakly induced by high osmolarity but not by trehalose. Expression is partially dependent on RpoS. HAMAP-Rule MF_01059
Sequence similarities	Belongs to the glycosyl hydrolase 37 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.0. HAMAP-Rule MF_01059

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular hyperosmotic response Inferred from expression pattern PubMed 17015655. Source: EcoCyc trehalose catabolic process Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha,alpha-trehalase activity Inferred from direct assay Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Cytoplasmic trehalase HAMAP-Rule MF_01059		PRO_0000173786

Sequences

	Sequence		Length	Mass (Da)
	P62601 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 543B82A7FAD4CB9D	FASTA	549	63,697
	10 20 30 40 50 60 MLNQKIQNPN PDELMIEVDL CYELDPYELK LDEMIEAEPE PEMIEGLPAS DALTPADRYL 70 80 90 100 110 120 ELFEHVQSAK IFPDSKTFPD CAPKMDPLDI LIRYRKVRRH RDFDLRKFVE NHFWLPEVYS 130 140 150 160 170 180 SEYVSDPQNS LKEHIDQLWP VLTREPQDHI PWSSLLALPQ SYIVPGGRFS ETYYWDSYFT 190 200 210 220 230 240 MLGLAESGRE DLLKCMADNF AWMIENYGHI PNGNRTYYLS RSQPPVFALM VELFEEDGVR 250 260 270 280 290 300 GARRYLDHLK MEYAFWMDGA ESLIPNQAYR HVVRMPDGSL LNRYWDDRDT PRDESWLEDV 310 320 330 340 350 360 ETAKHSGRPP NEVYRDLRAG AASGWDYSSR WLRDTGRLAS IRTTQFIPID LNAFLFKLES 370 380 390 400 410 420 AIANISALKG EKETEALFRQ KASARRDAVN RYLWDDENGI YRDYDWRREQ LALFSAAAIV 430 440 450 460 470 480 PLYVGMANHE QADRLANAVR SRLLTPGGIL ASEYETGEQW DKPNGWAPLQ WMAIQGFKMY 490 500 510 520 530 540 GDDLLGDEIA RSWLKTVNQF YLEQHKLIEK YHIADGVPRE GGGGEYPLQD GFGWTNGVVR RLIGLYGEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Characterization of a cytoplasmic trehalase of Escherichia coli." Horlacher R., Uhland K., Klein W., Ehrmann M., Boos W. J. Bacteriol. 178:6250-6257(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF N-TERMINUS. Strain: DHB4 and K12 / MC4100 / ATCC 35695 / DSM 6574.
[5]	"Determinants of translocation and folding of TreF, a trehalase of Escherichia coli." Uhland K., Mondigler M., Spiess C., Prinz W., Ehrmann M. J. Biol. Chem. 275:23439-23445(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: DHB3 and K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00039 Genomic DNA. Translation: AAB18495.1. U00096 Genomic DNA. Translation: AAC76544.1. AP009048 Genomic DNA. Translation: BAE77775.1.
PIR	S47739.
RefSeq	NP_417976.1. NC_000913.2. YP_491916.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P62601.
SMR	P62601. Positions 62-545.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62601. 2 interactions.
STRING	511145.b3519.
Proteomic databases
PRIDE	P62601.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76544; AAC76544; b3519. BAE77775; BAE77775; BAE77775.
GeneID	12931781. 948037.
KEGG	ecj:Y75_p3658. eco:b3519.
PATRIC	32122500. VBIEscCol129921_3628.
Organism-specific databases
EchoBASE	EB2156.
EcoGene	EG12245. treF.
Phylogenomic databases
eggNOG	COG1626.
HOGENOM	HOG000215464.
KO	K01194.
OMA	FWMDGAD.
ProtClustDB	PRK13270.
Enzyme and pathway databases
BioCyc	EcoCyc:TREHALACYTO-MONOMER. ECOL316407:JW3487-MONOMER. MetaCyc:TREHALACYTO-MONOMER.
UniPathway	UPA00300; UER00535.
Gene expression databases
Genevestigator	P62601.
Family and domain databases
HAMAP	MF_01059. Cyt_trehalase.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR023715. Cyt_trehalase. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. [Graphical view]
PANTHER	PTHR23403. PTHR23403. 1 hit.
Pfam	PF01204. Trehalase. 1 hit. [Graphical view]
PRINTS	PR00744. GLHYDRLASE37.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TREF_ECOLI

Accession

Primary (citable) accession number: P62601
Secondary accession number(s): P37196, Q2M7I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program