Reviewed, UniProtKB/Swiss-Prot P62594 (BLAT_SALTI)

Names and origin

Protein names

Recommended name:
    Beta-lactamase TEM
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:	bla
Ordered Locus Names:	HCM1.216

Encoded on

Plasmid pHCM1

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 286	263	Beta-lactamase TEM		PRO_0000016979
Regions
	Region	232 – 234	3	Substrate binding By similarity
Sites
	Active site	68	1	Acyl-ester intermediate By similarity
	Active site	166	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	75 ↔ 121		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62594-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: BB678943BB18934B	FASTA	286	31,515
	10 20 30 40 50 60 MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP 70 80 90 100 110 120 EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL 130 140 150 160 170 180 CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM 190 200 210 220 230 240 PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS 250 260 270 280 RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL513383 Genomic DNA. Translation: CAD09800.1.
RefSeq	NP_569411.1.
3D structure databases
SMR	P62594. Positions 24-286.
ModBase	Search...
Proteomic databases
PRIDE	P62594.
Genome annotation databases
GeneID	1251335.
GenomeReviews	Gene locus HCM1.216 in contig AL513383_GR.
KEGG	sty:HCM1.216.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P62594.
OMA	RVGDKTG.
Enzyme and pathway databases
BRENDA	3.5.2.6. 3716.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAT_SALTI

Accession

Primary (citable) accession number: P62594
Secondary accession number(s): P00810, Q47313

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)