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Protein

Beta-lactamase TEM

Gene

bla

more
Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Acyl-ester intermediate1
Active sitei166Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 2026.
SABIO-RKP62593.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase TEM (EC:3.5.2.6)
Alternative name(s):
IRT-4
Penicillinase
TEM-1
TEM-16/CAZ-7
TEM-2
TEM-24/CAZ-6
TEM-3
TEM-4
TEM-5
TEM-6
TEM-8/CAZ-2
Gene namesi
Name:bla
AND
Name:blaT-3
AND
Name:blaT-4
AND
Name:blaT-5
AND
Name:blaT-6
Encoded oniPlasmid R1 (R7268)2 Publications
Plasmid IncFII R100 (NR1)1 Publication
Plasmid R6K1 Publication
Plasmid pUD161 Publication
Plasmid pCFF042 Publications
Plasmid pCFF140 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Biotechnological usei

This protein is used as a marker in many commonly used cloning vectors, such as pBR322 and the pUC series.

Chemistry databases

ChEMBLiCHEMBL2065.
DrugBankiDB09060. Avibactam.
DB01053. Benzylpenicillin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000001697824 – 286Beta-lactamase TEMAdd BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 121

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP62593.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
P358042EBI-1031989,EBI-1031985From a different organism.

Protein-protein interaction databases

IntActiP62593. 1 interactor.

Chemistry databases

BindingDBiP62593.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 38Combined sources14
Beta strandi40 – 48Combined sources9
Turni49 – 51Combined sources3
Beta strandi54 – 59Combined sources6
Helixi67 – 69Combined sources3
Helixi70 – 83Combined sources14
Helixi97 – 99Combined sources3
Helixi107 – 109Combined sources3
Turni111 – 113Combined sources3
Helixi117 – 126Combined sources10
Helixi130 – 139Combined sources10
Helixi143 – 152Combined sources10
Helixi166 – 168Combined sources3
Turni171 – 173Combined sources3
Helixi181 – 193Combined sources13
Beta strandi194 – 197Combined sources4
Helixi199 – 210Combined sources12
Beta strandi213 – 215Combined sources3
Turni216 – 218Combined sources3
Helixi219 – 222Combined sources4
Beta strandi228 – 235Combined sources8
Turni237 – 239Combined sources3
Beta strandi241 – 249Combined sources9
Beta strandi255 – 263Combined sources9
Helixi268 – 284Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXBX-ray2.00A24-286[»]
1BT5X-ray1.80A24-286[»]
1BTLX-ray1.80A24-286[»]
1CK3X-ray2.28A24-284[»]
1ERMX-ray1.70A24-286[»]
1EROX-ray2.10A24-286[»]
1ERQX-ray1.90A24-286[»]
1ESUX-ray2.00A24-284[»]
1FQGX-ray1.70A24-286[»]
1JTDX-ray2.30A24-286[»]
1JTGX-ray1.73A/C24-286[»]
1JVJX-ray1.73A24-286[»]
1JWPX-ray1.75A24-286[»]
1JWVX-ray1.85A24-286[»]
1JWZX-ray1.80A24-286[»]
1LHYX-ray2.00A24-284[»]
1LI0X-ray1.61A24-284[»]
1LI9X-ray1.52A24-284[»]
1M40X-ray0.85A24-286[»]
1NXYX-ray1.60A24-286[»]
1NY0X-ray1.75A24-286[»]
1NYMX-ray1.20A24-286[»]
1NYYX-ray1.90A24-286[»]
1PZOX-ray1.90A24-284[»]
1PZPX-ray1.45A24-284[»]
1S0WX-ray2.30A/B24-286[»]
1TEMX-ray1.95A24-286[»]
1XPBX-ray1.90A24-286[»]
1XXMX-ray1.90A/B24-286[»]
1YT4X-ray1.40A24-284[»]
1ZG4X-ray1.55A1-284[»]
1ZG6X-ray2.10A1-284[»]
2B5RX-ray1.65A/B24-286[»]
2V1ZX-ray1.60A25-38[»]
A41-286[»]
2V20X-ray1.67A25-38[»]
A41-286[»]
3C7UX-ray2.20A/C24-286[»]
3C7VX-ray2.07A/C24-286[»]
3CMZX-ray1.92A24-286[»]
3DTMX-ray2.00A24-286[»]
3JYIX-ray2.70A/B/C/D/E/F24-286[»]
3TOIX-ray1.90A/B39-283[»]
4DXBX-ray2.29A/B24-226[»]
4DXCX-ray2.30A24-226[»]
4GKUX-ray1.92A24-286[»]
4IBRX-ray2.20A24-286[»]
4IBXX-ray2.68A/B/C/D/E24-286[»]
4ID4X-ray1.05A24-147[»]
A189-286[»]
4MEZX-ray2.05A/B24-286[»]
4QY5X-ray1.50A24-147[»]
A189-286[»]
4QY6X-ray1.15A24-147[»]
A189-286[»]
4R4RX-ray1.20A24-147[»]
A189-286[»]
4R4SX-ray1.10A24-149[»]
A189-286[»]
4RVAX-ray1.44A24-286[»]
4RX2X-ray2.31A/B/C/D/E/F/G/H24-286[»]
4RX3X-ray1.39A24-286[»]
4ZJ1X-ray1.54A1-286[»]
4ZJ2X-ray1.80A1-286[»]
4ZJ3X-ray1.70A1-286[»]
ProteinModelPortaliP62593.
SMRiP62593.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62593.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni232 – 234Substrate binding3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18698.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN
60 70 80 90 100
SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL
110 120 130 140 150
VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL
160 170 180 190 200
HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA
210 220 230 240 250
SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD
260 270 280
GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Length:286
Mass (Da):31,515
Last modified:July 21, 1986 - v1
Checksum:iBB678943BB18934B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti19L → F in TEM-4. 1
Natural varianti37Q → K in TEM-2, TEM-3, TEM-8, TEM-16 and TEM-24. 1
Natural varianti67M → L in IRT-4. 1
Natural varianti102E → K in TEM-3, TEM-4, TEM-6, TEM-8, TEM-16 and TEM-24. 1
Natural varianti162R → H in TEM-6 and TEM-16. 1
Natural varianti162R → S in TEM-5, TEM-8 and TEM-24. 1
Natural varianti235A → T in TEM-5 and TEM-24. 1
Natural varianti236G → S in TEM-3, TEM-4 and TEM-8. 1
Natural varianti237E → K in TEM-5 and TEM-24. 1
Natural varianti261T → M in TEM-4. 1
Natural varianti272N → D in IRT-4. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01749 Genomic DNA. Translation: AAB59737.1.
V00613 Genomic DNA. Translation: CAA23886.1.
X64523 Genomic DNA. Translation: CAA45828.1.
X57972 Genomic DNA. Translation: CAA41038.1.
X65252 Genomic DNA. Translation: CAA46344.1.
X65253 Genomic DNA. Translation: CAA46345.1.
X65254 Genomic DNA. Translation: CAA46346.1.
U89928 Genomic DNA. Translation: AAB64386.1.
U66885 Genomic DNA. Translation: AAC48875.1.
PIRiA93821. PNECP.
S30113.
RefSeqiNP_943295.1. NC_005248.1.
NP_957565.1. NC_005327.1.
WP_000027057.1. NZ_MCBU01000312.1.
WP_063864949.1. NG_050277.1.
YP_001096393.1. NC_009132.1.
YP_001693174.1. NC_010378.1.
YP_001816609.1. NC_010558.1.
YP_003108102.1. NC_013120.1.
YP_003108210.1. NC_013121.1.
YP_003829069.1. NC_014383.1.
YP_003829170.1. NC_014384.1.
YP_003937675.1. NC_014615.1.
YP_004119720.1. NC_014843.1.
YP_004119734.1. NC_014843.1.
YP_006903139.1. NC_019047.1.
YP_006939984.1. NC_018994.1.
YP_006940092.1. NC_018995.1.
YP_006952181.1. NC_019056.1.
YP_006952421.1. NC_019062.1.
YP_006952427.1. NC_019063.1.
YP_006953479.1. NC_019073.1.
YP_006953762.1. NC_019088.1.
YP_006953988.1. NC_019091.1.
YP_006954235.1. NC_019095.1.
YP_007447512.1. NC_020278.2.
YP_008864019.1. NC_022992.1.
YP_008864686.1. NC_022996.1.
YP_008995211.1. NC_023277.2.
YP_009060387.1. NC_024960.1.
YP_009060444.1. NC_024961.1.
YP_009060580.1. NC_024967.1.
YP_009061316.1. NC_024977.1.
YP_009066523.1. NC_025106.1.
YP_009068284.1. NC_025139.1.
YP_009068507.1. NC_025141.1.
YP_009070232.1. NC_025167.1.
YP_009070558.1. NC_025175.1.
YP_009071512.1. NC_025183.1.
YP_190222.1. NC_006671.1.

Genome annotation databases

GeneIDi10076131.
10076142.
13876868.
13877052.
13903673.
13905334.
13905363.
13906404.
13906709.
13906924.
13909533.
13909568.
14612524.
17824300.
17824435.
18157686.
20466965.
20466993.
20467118.
20468340.
20471961.
20491414.
20491639.
20492529.
20492626.
20493584.
2716540.
3244915.
3722457.
4924718.
5961992.
6276043.
8319064.
8319163.
9537966.
9538101.
9846067.
KEGGiag:AAB59737.
ag:CAA41038.
ag:CAA45828.
ag:CAA46344.
ag:CAA46345.
ag:CAA46346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01749 Genomic DNA. Translation: AAB59737.1.
V00613 Genomic DNA. Translation: CAA23886.1.
X64523 Genomic DNA. Translation: CAA45828.1.
X57972 Genomic DNA. Translation: CAA41038.1.
X65252 Genomic DNA. Translation: CAA46344.1.
X65253 Genomic DNA. Translation: CAA46345.1.
X65254 Genomic DNA. Translation: CAA46346.1.
U89928 Genomic DNA. Translation: AAB64386.1.
U66885 Genomic DNA. Translation: AAC48875.1.
PIRiA93821. PNECP.
S30113.
RefSeqiNP_943295.1. NC_005248.1.
NP_957565.1. NC_005327.1.
WP_000027057.1. NZ_MCBU01000312.1.
WP_063864949.1. NG_050277.1.
YP_001096393.1. NC_009132.1.
YP_001693174.1. NC_010378.1.
YP_001816609.1. NC_010558.1.
YP_003108102.1. NC_013120.1.
YP_003108210.1. NC_013121.1.
YP_003829069.1. NC_014383.1.
YP_003829170.1. NC_014384.1.
YP_003937675.1. NC_014615.1.
YP_004119720.1. NC_014843.1.
YP_004119734.1. NC_014843.1.
YP_006903139.1. NC_019047.1.
YP_006939984.1. NC_018994.1.
YP_006940092.1. NC_018995.1.
YP_006952181.1. NC_019056.1.
YP_006952421.1. NC_019062.1.
YP_006952427.1. NC_019063.1.
YP_006953479.1. NC_019073.1.
YP_006953762.1. NC_019088.1.
YP_006953988.1. NC_019091.1.
YP_006954235.1. NC_019095.1.
YP_007447512.1. NC_020278.2.
YP_008864019.1. NC_022992.1.
YP_008864686.1. NC_022996.1.
YP_008995211.1. NC_023277.2.
YP_009060387.1. NC_024960.1.
YP_009060444.1. NC_024961.1.
YP_009060580.1. NC_024967.1.
YP_009061316.1. NC_024977.1.
YP_009066523.1. NC_025106.1.
YP_009068284.1. NC_025139.1.
YP_009068507.1. NC_025141.1.
YP_009070232.1. NC_025167.1.
YP_009070558.1. NC_025175.1.
YP_009071512.1. NC_025183.1.
YP_190222.1. NC_006671.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AXBX-ray2.00A24-286[»]
1BT5X-ray1.80A24-286[»]
1BTLX-ray1.80A24-286[»]
1CK3X-ray2.28A24-284[»]
1ERMX-ray1.70A24-286[»]
1EROX-ray2.10A24-286[»]
1ERQX-ray1.90A24-286[»]
1ESUX-ray2.00A24-284[»]
1FQGX-ray1.70A24-286[»]
1JTDX-ray2.30A24-286[»]
1JTGX-ray1.73A/C24-286[»]
1JVJX-ray1.73A24-286[»]
1JWPX-ray1.75A24-286[»]
1JWVX-ray1.85A24-286[»]
1JWZX-ray1.80A24-286[»]
1LHYX-ray2.00A24-284[»]
1LI0X-ray1.61A24-284[»]
1LI9X-ray1.52A24-284[»]
1M40X-ray0.85A24-286[»]
1NXYX-ray1.60A24-286[»]
1NY0X-ray1.75A24-286[»]
1NYMX-ray1.20A24-286[»]
1NYYX-ray1.90A24-286[»]
1PZOX-ray1.90A24-284[»]
1PZPX-ray1.45A24-284[»]
1S0WX-ray2.30A/B24-286[»]
1TEMX-ray1.95A24-286[»]
1XPBX-ray1.90A24-286[»]
1XXMX-ray1.90A/B24-286[»]
1YT4X-ray1.40A24-284[»]
1ZG4X-ray1.55A1-284[»]
1ZG6X-ray2.10A1-284[»]
2B5RX-ray1.65A/B24-286[»]
2V1ZX-ray1.60A25-38[»]
A41-286[»]
2V20X-ray1.67A25-38[»]
A41-286[»]
3C7UX-ray2.20A/C24-286[»]
3C7VX-ray2.07A/C24-286[»]
3CMZX-ray1.92A24-286[»]
3DTMX-ray2.00A24-286[»]
3JYIX-ray2.70A/B/C/D/E/F24-286[»]
3TOIX-ray1.90A/B39-283[»]
4DXBX-ray2.29A/B24-226[»]
4DXCX-ray2.30A24-226[»]
4GKUX-ray1.92A24-286[»]
4IBRX-ray2.20A24-286[»]
4IBXX-ray2.68A/B/C/D/E24-286[»]
4ID4X-ray1.05A24-147[»]
A189-286[»]
4MEZX-ray2.05A/B24-286[»]
4QY5X-ray1.50A24-147[»]
A189-286[»]
4QY6X-ray1.15A24-147[»]
A189-286[»]
4R4RX-ray1.20A24-147[»]
A189-286[»]
4R4SX-ray1.10A24-149[»]
A189-286[»]
4RVAX-ray1.44A24-286[»]
4RX2X-ray2.31A/B/C/D/E/F/G/H24-286[»]
4RX3X-ray1.39A24-286[»]
4ZJ1X-ray1.54A1-286[»]
4ZJ2X-ray1.80A1-286[»]
4ZJ3X-ray1.70A1-286[»]
ProteinModelPortaliP62593.
SMRiP62593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62593. 1 interactor.

Chemistry databases

BindingDBiP62593.
ChEMBLiCHEMBL2065.
DrugBankiDB09060. Avibactam.
DB01053. Benzylpenicillin.

Proteomic databases

PRIDEiP62593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi10076131.
10076142.
13876868.
13877052.
13903673.
13905334.
13905363.
13906404.
13906709.
13906924.
13909533.
13909568.
14612524.
17824300.
17824435.
18157686.
20466965.
20466993.
20467118.
20468340.
20471961.
20491414.
20491639.
20492529.
20492626.
20493584.
2716540.
3244915.
3722457.
4924718.
5961992.
6276043.
8319064.
8319163.
9537966.
9538101.
9846067.
KEGGiag:AAB59737.
ag:CAA41038.
ag:CAA45828.
ag:CAA46344.
ag:CAA46345.
ag:CAA46346.

Phylogenomic databases

KOiK18698.

Enzyme and pathway databases

BRENDAi3.5.2.6. 2026.
SABIO-RKP62593.

Miscellaneous databases

EvolutionaryTraceiP62593.
PROiP62593.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAT_ECOLX
AccessioniPrimary (citable) accession number: P62593
Secondary accession number(s): P00810, Q47313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The beta-lactamase present on pBR322 was cloned from plasmid R1 (R7268).

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.