P62580 (CAT_SALTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloramphenicol acetyltransferase
Short name=CAT
EC=2.3.1.28

Gene names

Name:	cat
Ordered Locus Names:	HCM1.206

Encoded on

Plasmid pHCM1

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is an effector of chloramphenicol resistance in bacteria.
Catalytic activity	Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Belongs to the chloramphenicol acetyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chloramphenicol O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Chloramphenicol acetyltransferase		PRO_0000165867
Sites
	Active site	193	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62580 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 02C92576273FA18B	FASTA	219	25,663
	10 20 30 40 50 60 MEKKITGYTT VDISQWHRKE HFEAFQSVAQ CTYNQTVQLD ITAFLKTVKK NKHKFYPAFI 70 80 90 100 110 120 HILARLMNAH PEFRMAMKDG ELVIWDSVHP CYTVFHEQTE TFSSLWSEYH DDFRQFLHIY 130 140 150 160 170 180 SQDVACYGEN LAYFPKGFIE NMFFVSANPW VSFTSFDLNV ANMDNFFAPV FTMGKYYTQG 190 200 210 DKVLMPLAIQ VHHAVCDGFH VGRMLNELQQ YCDEWQGGA

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL513383 Genomic DNA. Translation: CAD09793.1.
RefSeq	NP_569406.1. NC_003384.1.
3D structure databases
ProteinModelPortal	P62580.
SMR	P62580. Positions 2-218.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1251342.
GenomeReviews	Gene locus HCM1.206 in contig AL513383_GR.
KEGG	sty:HCM1.206.
PATRIC	18548517. VBISalEnt120419_5228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG452557.
OMA	PISSIPW.
ProtClustDB	PRK13757.
Family and domain databases
InterPro	IPR023213. CAT-like_dom. IPR018372. Chloramphenicol_AcTrfase_AS. IPR001707. Cmp_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.30.559.10. CAT-like_dom. 1 hit.
KO	K00638.
Pfam	PF00302. CAT. 1 hit. [Graphical view]
PIRSF	PIRSF000440. CAT. 1 hit.
ProDom	PD002660. Cmp_AcTrfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM01059. CAT. 1 hit. [Graphical view]
PROSITE	PS00100. CAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB02703. Fusidic Acid.

Entry information

Entry name

CAT_SALTI

Accession

Primary (citable) accession number: P62580
Secondary accession number(s): P00483

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program