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Protein

Chloramphenicol acetyltransferase

Gene

cat

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is an effector of chloramphenicol resistance in bacteria.

Miscellaneous

Transposon Tncam204 is derived from the R plasmid NR1.1 Publication

Catalytic activityi

Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei193Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processAntibiotic resistance

Enzyme and pathway databases

BRENDAi2.3.1.28 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol acetyltransferase (EC:2.3.1.28)
Short name:
CAT
Gene namesi
Name:cat
Encoded oniPlasmid JR66B1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Biotechnological usei

This protein is used as a marker in many commonly used cloning vectors, such as pACYC184.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658651 – 219Chloramphenicol acetyltransferaseAdd BLAST219

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

IntActiP62577, 1 interactor
MINTiP62577

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Helixi13 – 15Combined sources3
Helixi19 – 25Combined sources7
Turni26 – 29Combined sources4
Beta strandi31 – 40Combined sources10
Helixi42 – 50Combined sources9
Helixi55 – 67Combined sources13
Helixi71 – 73Combined sources3
Beta strandi74 – 78Combined sources5
Beta strandi81 – 86Combined sources6
Beta strandi89 – 96Combined sources8
Turni97 – 100Combined sources4
Beta strandi101 – 106Combined sources6
Helixi113 – 127Combined sources15
Beta strandi132 – 134Combined sources3
Beta strandi140 – 147Combined sources8
Beta strandi154 – 161Combined sources8
Beta strandi170 – 173Combined sources4
Beta strandi177 – 179Combined sources3
Beta strandi182 – 192Combined sources11
Turni193 – 195Combined sources3
Helixi198 – 214Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NOCX-ray2.60B1-219[»]
1PD5X-ray2.50A/B/C/D/E/F/G/H/I/J/K/L1-219[»]
1Q23X-ray2.18A/B/C/D/E/F/G/H/I/J/K/L1-219[»]
3U9BX-ray3.20A/B/C/D/E/F/G/H/I1-219[»]
3U9FX-ray2.90A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/R/S1-219[»]
ProteinModelPortaliP62577
SMRiP62577
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62577

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19271

Family and domain databases

Gene3Di3.30.559.10, 1 hit
InterProiView protein in InterPro
IPR023213 CAT-like_dom_sf
IPR018372 Chloramphenicol_AcTrfase_AS
IPR001707 Cmp_AcTrfase
PANTHERiPTHR38474 PTHR38474, 1 hit
PfamiView protein in Pfam
PF00302 CAT, 1 hit
PIRSFiPIRSF000440 CAT, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002660 Cmp_AcTrfase, 1 hit
SMARTiView protein in SMART
SM01059 CAT, 1 hit
PROSITEiView protein in PROSITE
PS00100 CAT, 1 hit

Sequencei

Sequence statusi: Complete.

P62577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKITGYTT VDISQWHRKE HFEAFQSVAQ CTYNQTVQLD ITAFLKTVKK
60 70 80 90 100
NKHKFYPAFI HILARLMNAH PEFRMAMKDG ELVIWDSVHP CYTVFHEQTE
110 120 130 140 150
TFSSLWSEYH DDFRQFLHIY SQDVACYGEN LAYFPKGFIE NMFFVSANPW
160 170 180 190 200
VSFTSFDLNV ANMDNFFAPV FTMGKYYTQG DKVLMPLAIQ VHHAVCDGFH
210
VGRMLNELQQ YCDEWQGGA
Length:219
Mass (Da):25,663
Last modified:July 21, 1986 - v1
Checksum:i02C92576273FA18B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00623 Genomic DNA Translation: CAA23900.1
V00622 Genomic DNA Translation: CAA23899.1
PIRiA93220 XXECC1
RefSeqiWP_000412211.1, NZ_PJDH01000359.1
YP_001096419.1, NC_009133.1
YP_001816591.1, NC_010558.1
YP_008995272.1, NC_023277.2
YP_008997430.1, NC_023289.2
YP_009068571.1, NC_025141.1
YP_009071093.1, NC_025179.1
YP_009071408.1, NC_025181.1
YP_025721.1, NC_005923.1

Genome annotation databases

GeneIDi18157747
18157952
20491703
20493165
23335835
2847485
4924744
6276004
KEGGiag:CAA23899

Similar proteinsi

Entry informationi

Entry nameiCAT_ECOLX
AccessioniPrimary (citable) accession number: P62577
Secondary accession number(s): P00483
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid, Transposable element
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Main funding by: National Institutes of Health