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P62575 (NANA_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase A
EC=3.2.1.18
Alternative name(s):
Neuraminidase A
Gene names
Name:nanA
OrganismStreptococcus pneumoniae
Taxonomic identifier1313 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Secreted › cell wall; Peptidoglycan-anchor Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5353 Potential
Chain54 – 1006953Sialidase A
PRO_0000012034
Propeptide1007 – 103529Removed by sortase Potential
PRO_0000012035

Regions

Repeat381 – 39212BNR 1
Repeat539 – 55012BNR 2
Repeat607 – 61812BNR 3
Repeat672 – 68312BNR 4
Motif1003 – 10075LPXTG sorting signal Potential

Sites

Active site3721Proton acceptor By similarity
Active site6471 By similarity
Binding site3471Substrate By similarity
Binding site6631Substrate By similarity

Amino acid modifications

Modified residue10061Pentaglycyl murein peptidoglycan amidated threonine Potential

Secondary structure

1 ............................................................. 1035
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P62575 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: C5B8A2D7A12E12F3

FASTA1,035114,742
        10         20         30         40         50         60 
MSYFRNRDID IERNSMNRSV QERKCRYSIR KLSVGAVSMI VGAVVFGTSP VLAQEGASEQ 

        70         80         90        100        110        120 
PLANETQLSG ESSTLTDTEK SQPSSETELS GNKQEQERKD KQEEKIPRDY YARDLENVET 

       130        140        150        160        170        180 
VIEKEDVETN ASNGQRVDLS SELDKLKKLE NATVHMEFKP DAKAPAFYNL FSVSSATKKD 

       190        200        210        220        230        240 
EYFTMAVYNN TATLEGRGSD GKQFYNNYND APLKVKPGQW NSVTFTVEKP TAELPKGRVR 

       250        260        270        280        290        300 
LYVNGVLSRT SLRSGNFIKD MPDVTHVQIG ATKRANNTVW GSNLQIRNLT VYNRALTPEE 

       310        320        330        340        350        360 
VQKRSQLFKR SDLEKKLPEG AALTEKTDIF ESGRNGKPNK DGIKSYRIPA LLKTDKGTLI 

       370        380        390        400        410        420 
AGADERRLHS SDWGDIGMVI RRSEDNGKTW GDRVTITNLR DNPKASDPSI GSPVNIDMVL 

       430        440        450        460        470        480 
VQDPETKRIF SIYDMFPEGK GIFGMSSQKE EAYKKIDGKT YQILYREGEK GAYTIRENGT 

       490        500        510        520        530        540 
VYTPDGKATD YRVVVDPVKP AYSDKGDLYK GNQLLGNIYF TTNKTSPFRI AKDSYLWMSY 

       550        560        570        580        590        600 
SDDDGKTWSA PQDITPMVKA DWMKFLGVGP GTGIVLRNGP HKGRILIPVY TTNNVSHLNG 

       610        620        630        640        650        660 
SQSSRIIYSD DHGKTWHAGE AVNDNRQVDG QKIHSSTMNN RRAQNTESTV VQLNNGDVKL 

       670        680        690        700        710        720 
FMRGLTGDLQ VATSKDGGVT WEKDIKRYPQ VKDVYVQMSA IHTMHEGKEY IILSNAGGPK 

       730        740        750        760        770        780 
RENGMVHLAR VEENGELTWL KHNPIQKGEF AYNSLQELGN GEYGILYEHT EKGQNAYTLS 

       790        800        810        820        830        840 
FRKFNWDFLS KDLISPTEAK VKRTREMGKG VIGLEFDSEV LVNKAPTLQL ANGKTARFMT 

       850        860        870        880        890        900 
QYDTKTLLFT VDSEDMGQKV TGLAEGAIES MHNLPVSVAG TKLSNGMNGS EAAVHEVPEY 

       910        920        930        940        950        960 
TGPLGTSGEE PAPTVEKPEY TGPLGTSGEE PAPTVEKPEY TGPLGTAGEE AAPTVEKPEF 

       970        980        990       1000       1010       1020 
TGGVNGTEPA VHEIAEYKGS DSLVTLTTKE DYTYKAPLAQ QALPETGNKE SDLLASLGLT 

      1030 
AFFLGLFTLG KKREQ

« Hide

References

[1]"A neuraminidase from Streptococcus pneumoniae has the features of a surface protein."
Camara M., Boulnois G.J., Andrew P.W., Mitchell T.J.
Infect. Immun. 62:3688-3695(1994) [PubMed: 8063384] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: R36A / NCTC 10319.
[2]"Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
Berry A.M., Lock R.A., Paton J.C.
J. Bacteriol. 178:4854-4860(1996) [PubMed: 8759848] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 882-1035.
Strain: Serotype 6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X72967 Genomic DNA. Translation: CAA51473.1.
U43526 Genomic DNA. Translation: AAC44391.1.
PIRT30287.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2VVZX-ray2.50A/B319-822[»]
2YA4X-ray1.80A/B318-791[»]
2YA5X-ray2.00A/B318-791[»]
2YA6X-ray2.00A/B318-791[»]
2YA7X-ray1.89A/B/C/D318-791[»]
2YA8X-ray1.75A/B318-791[»]
ProteinModelPortalP62575.
SMRP62575. Positions 322-791.
ModBaseSearch...

Protein family/group databases

CAZyCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR005877. Gpos_YSIRK.
IPR001791. Laminin_G.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR011040. Neuraminidase.
IPR001899. Surface_protein_Gram_pos_cocci.
IPR023364. Trans_sialidase_dom3.
[Graphical view]
Gene3DG3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
G3DSA:2.40.220.10. G3DSA:2.40.220.10. 1 hit.
G3DSA:2.120.10.10. Neuraminidase. 2 hits.
PfamPF02012. BNR. 4 hits.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTSM00282. LamG. 1 hit.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 1 hit.
SSF50939. Sialidase. 1 hit.
TIGRFAMsTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNANA_STRPN
AccessionPrimary (citable) accession number: P62575
Secondary accession number(s): Q54722, Q59959
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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