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Protein

Sialidase A

Gene

nanA

Organism
Streptococcus pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei347SubstrateBy similarity1
Active sitei372Proton acceptorBy similarity1
Active sitei647By similarity1
Binding sitei663SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.18. 1960.
SABIO-RKP62575.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase A (EC:3.2.1.18)
Alternative name(s):
Neuraminidase A
Gene namesi
Name:nanA
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 53Sequence analysisAdd BLAST53
ChainiPRO_000001203454 – 1006Sialidase AAdd BLAST953
PropeptideiPRO_00000120351007 – 1035Removed by sortasePROSITE-ProRule annotationAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1006Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Interactioni

Protein-protein interaction databases

STRINGi171101.spr1536.

Structurei

Secondary structure

11035
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi121 – 129Combined sources9
Helixi131 – 133Combined sources3
Beta strandi136 – 138Combined sources3
Helixi140 – 142Combined sources3
Helixi143 – 147Combined sources5
Beta strandi150 – 160Combined sources11
Beta strandi166 – 174Combined sources9
Beta strandi182 – 188Combined sources7
Beta strandi191 – 197Combined sources7
Beta strandi203 – 205Combined sources3
Beta strandi221 – 228Combined sources8
Beta strandi232 – 234Combined sources3
Beta strandi238 – 243Combined sources6
Beta strandi246 – 251Combined sources6
Helixi258 – 260Combined sources3
Beta strandi266 – 274Combined sources9
Beta strandi277 – 279Combined sources3
Beta strandi284 – 294Combined sources11
Helixi298 – 303Combined sources6
Beta strandi327 – 330Combined sources4
Beta strandi345 – 353Combined sources9
Beta strandi359 – 368Combined sources10
Beta strandi370 – 385Combined sources16
Beta strandi394 – 397Combined sources4
Helixi408 – 410Combined sources3
Beta strandi414 – 422Combined sources9
Turni424 – 426Combined sources3
Beta strandi429 – 436Combined sources8
Beta strandi438 – 440Combined sources3
Helixi441 – 444Combined sources4
Beta strandi453 – 456Combined sources4
Beta strandi459 – 466Combined sources8
Beta strandi473 – 475Combined sources3
Helixi477 – 479Combined sources3
Beta strandi480 – 482Combined sources3
Beta strandi488 – 493Combined sources6
Helixi500 – 502Combined sources3
Turni503 – 506Combined sources4
Beta strandi507 – 510Combined sources4
Beta strandi513 – 517Combined sources5
Beta strandi525 – 529Combined sources5
Beta strandi535 – 543Combined sources9
Helixi555 – 558Combined sources4
Beta strandi563 – 568Combined sources6
Turni580 – 583Combined sources4
Beta strandi585 – 593Combined sources9
Turni594 – 596Combined sources3
Helixi597 – 600Combined sources4
Beta strandi603 – 611Combined sources9
Turni622 – 625Combined sources4
Beta strandi626 – 628Combined sources3
Beta strandi631 – 633Combined sources3
Turni635 – 637Combined sources3
Helixi641 – 643Combined sources3
Beta strandi647 – 652Combined sources6
Beta strandi658 – 662Combined sources5
Beta strandi665 – 680Combined sources16
Beta strandi686 – 692Combined sources7
Beta strandi699 – 705Combined sources7
Beta strandi708 – 716Combined sources9
Beta strandi718 – 731Combined sources14
Beta strandi733 – 735Combined sources3
Beta strandi737 – 749Combined sources13
Beta strandi753 – 759Combined sources7
Beta strandi762 – 769Combined sources8
Beta strandi778 – 785Combined sources8
Helixi786 – 790Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VVZX-ray2.50A/B319-822[»]
2YA4X-ray1.80A/B318-791[»]
2YA5X-ray2.00A/B318-791[»]
2YA6X-ray2.00A/B318-791[»]
2YA7X-ray1.89A/B/C/D318-791[»]
2YA8X-ray1.75A/B318-791[»]
4C1XX-ray1.84A121-305[»]
4ZXKX-ray1.84A/B121-305[»]
ProteinModelPortaliP62575.
SMRiP62575.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati381 – 392BNR 1Add BLAST12
Repeati539 – 550BNR 2Add BLAST12
Repeati607 – 618BNR 3Add BLAST12
Repeati672 – 683BNR 4Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1003 – 1007LPXTG sorting signalPROSITE-ProRule annotation5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108478. Bacteria.
COG4409. LUCA.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF13088. BNR_2. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYFRNRDID IERNSMNRSV QERKCRYSIR KLSVGAVSMI VGAVVFGTSP
60 70 80 90 100
VLAQEGASEQ PLANETQLSG ESSTLTDTEK SQPSSETELS GNKQEQERKD
110 120 130 140 150
KQEEKIPRDY YARDLENVET VIEKEDVETN ASNGQRVDLS SELDKLKKLE
160 170 180 190 200
NATVHMEFKP DAKAPAFYNL FSVSSATKKD EYFTMAVYNN TATLEGRGSD
210 220 230 240 250
GKQFYNNYND APLKVKPGQW NSVTFTVEKP TAELPKGRVR LYVNGVLSRT
260 270 280 290 300
SLRSGNFIKD MPDVTHVQIG ATKRANNTVW GSNLQIRNLT VYNRALTPEE
310 320 330 340 350
VQKRSQLFKR SDLEKKLPEG AALTEKTDIF ESGRNGKPNK DGIKSYRIPA
360 370 380 390 400
LLKTDKGTLI AGADERRLHS SDWGDIGMVI RRSEDNGKTW GDRVTITNLR
410 420 430 440 450
DNPKASDPSI GSPVNIDMVL VQDPETKRIF SIYDMFPEGK GIFGMSSQKE
460 470 480 490 500
EAYKKIDGKT YQILYREGEK GAYTIRENGT VYTPDGKATD YRVVVDPVKP
510 520 530 540 550
AYSDKGDLYK GNQLLGNIYF TTNKTSPFRI AKDSYLWMSY SDDDGKTWSA
560 570 580 590 600
PQDITPMVKA DWMKFLGVGP GTGIVLRNGP HKGRILIPVY TTNNVSHLNG
610 620 630 640 650
SQSSRIIYSD DHGKTWHAGE AVNDNRQVDG QKIHSSTMNN RRAQNTESTV
660 670 680 690 700
VQLNNGDVKL FMRGLTGDLQ VATSKDGGVT WEKDIKRYPQ VKDVYVQMSA
710 720 730 740 750
IHTMHEGKEY IILSNAGGPK RENGMVHLAR VEENGELTWL KHNPIQKGEF
760 770 780 790 800
AYNSLQELGN GEYGILYEHT EKGQNAYTLS FRKFNWDFLS KDLISPTEAK
810 820 830 840 850
VKRTREMGKG VIGLEFDSEV LVNKAPTLQL ANGKTARFMT QYDTKTLLFT
860 870 880 890 900
VDSEDMGQKV TGLAEGAIES MHNLPVSVAG TKLSNGMNGS EAAVHEVPEY
910 920 930 940 950
TGPLGTSGEE PAPTVEKPEY TGPLGTSGEE PAPTVEKPEY TGPLGTAGEE
960 970 980 990 1000
AAPTVEKPEF TGGVNGTEPA VHEIAEYKGS DSLVTLTTKE DYTYKAPLAQ
1010 1020 1030
QALPETGNKE SDLLASLGLT AFFLGLFTLG KKREQ
Length:1,035
Mass (Da):114,742
Last modified:July 19, 2004 - v1
Checksum:iC5B8A2D7A12E12F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1.
U43526 Genomic DNA. Translation: AAC44391.1.
PIRiT30287.
RefSeqiWP_010976603.1. NZ_LJVS01000130.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1.
U43526 Genomic DNA. Translation: AAC44391.1.
PIRiT30287.
RefSeqiWP_010976603.1. NZ_LJVS01000130.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VVZX-ray2.50A/B319-822[»]
2YA4X-ray1.80A/B318-791[»]
2YA5X-ray2.00A/B318-791[»]
2YA6X-ray2.00A/B318-791[»]
2YA7X-ray1.89A/B/C/D318-791[»]
2YA8X-ray1.75A/B318-791[»]
4C1XX-ray1.84A121-305[»]
4ZXKX-ray1.84A/B121-305[»]
ProteinModelPortaliP62575.
SMRiP62575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi171101.spr1536.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108478. Bacteria.
COG4409. LUCA.

Enzyme and pathway databases

BRENDAi3.2.1.18. 1960.
SABIO-RKP62575.

Miscellaneous databases

EvolutionaryTraceiP62575.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF13088. BNR_2. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANA_STREE
AccessioniPrimary (citable) accession number: P62575
Secondary accession number(s): Q54722, Q59959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.