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P62575

- NANA_STREE

UniProt

P62575 - NANA_STREE

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Protein

Sialidase A

Gene

nanA

Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei347 – 3471SubstrateBy similarity
Active sitei372 – 3721Proton acceptorBy similarity
Active sitei647 – 6471By similarity
Binding sitei663 – 6631SubstrateBy similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP62575.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase A (EC:3.2.1.18)
Alternative name(s):
Neuraminidase A
Gene namesi
Name:nanA
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5353Sequence AnalysisAdd
BLAST
Chaini54 – 1006953Sialidase APRO_0000012034Add
BLAST
Propeptidei1007 – 103529Removed by sortasePROSITE-ProRule annotationPRO_0000012035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1006 – 10061Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

Keywords - PTMi

Peptidoglycan-anchor

Structurei

Secondary structure

1
1035
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi121 – 1299Combined sources
Helixi131 – 1333Combined sources
Beta strandi136 – 1383Combined sources
Helixi140 – 1423Combined sources
Helixi143 – 1475Combined sources
Beta strandi150 – 16011Combined sources
Beta strandi166 – 1749Combined sources
Beta strandi182 – 1887Combined sources
Beta strandi191 – 1977Combined sources
Beta strandi203 – 2053Combined sources
Beta strandi221 – 2288Combined sources
Beta strandi232 – 2343Combined sources
Beta strandi238 – 2436Combined sources
Beta strandi246 – 2516Combined sources
Helixi258 – 2603Combined sources
Beta strandi266 – 2749Combined sources
Beta strandi277 – 2793Combined sources
Beta strandi284 – 29411Combined sources
Helixi298 – 3036Combined sources
Beta strandi327 – 3304Combined sources
Beta strandi345 – 3539Combined sources
Beta strandi359 – 36810Combined sources
Beta strandi370 – 38516Combined sources
Beta strandi394 – 3974Combined sources
Helixi408 – 4103Combined sources
Beta strandi414 – 4229Combined sources
Turni424 – 4263Combined sources
Beta strandi429 – 4368Combined sources
Beta strandi438 – 4403Combined sources
Helixi441 – 4444Combined sources
Beta strandi453 – 4564Combined sources
Beta strandi459 – 4668Combined sources
Beta strandi473 – 4753Combined sources
Helixi477 – 4793Combined sources
Beta strandi480 – 4823Combined sources
Beta strandi488 – 4936Combined sources
Helixi500 – 5023Combined sources
Turni503 – 5064Combined sources
Beta strandi507 – 5104Combined sources
Beta strandi513 – 5175Combined sources
Beta strandi525 – 5295Combined sources
Beta strandi535 – 5439Combined sources
Helixi555 – 5584Combined sources
Beta strandi563 – 5686Combined sources
Turni580 – 5834Combined sources
Beta strandi585 – 5939Combined sources
Turni594 – 5963Combined sources
Helixi597 – 6004Combined sources
Beta strandi603 – 6119Combined sources
Turni622 – 6254Combined sources
Beta strandi626 – 6283Combined sources
Beta strandi631 – 6333Combined sources
Turni635 – 6373Combined sources
Helixi641 – 6433Combined sources
Beta strandi647 – 6526Combined sources
Beta strandi658 – 6625Combined sources
Beta strandi665 – 68016Combined sources
Beta strandi686 – 6927Combined sources
Beta strandi699 – 7057Combined sources
Beta strandi708 – 7169Combined sources
Beta strandi718 – 73114Combined sources
Beta strandi733 – 7353Combined sources
Beta strandi737 – 74913Combined sources
Beta strandi753 – 7597Combined sources
Beta strandi762 – 7698Combined sources
Beta strandi778 – 7858Combined sources
Helixi786 – 7905Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VVZX-ray2.50A/B319-822[»]
2YA4X-ray1.80A/B318-791[»]
2YA5X-ray2.00A/B318-791[»]
2YA6X-ray2.00A/B318-791[»]
2YA7X-ray1.89A/B/C/D318-791[»]
2YA8X-ray1.75A/B318-791[»]
4C1XX-ray1.84A121-305[»]
ProteinModelPortaliP62575.
SMRiP62575. Positions 322-791.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati381 – 39212BNR 1Add
BLAST
Repeati539 – 55012BNR 2Add
BLAST
Repeati607 – 61812BNR 3Add
BLAST
Repeati672 – 68312BNR 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1003 – 10075LPXTG sorting signalPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR019931. LPXTG_anchor.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62575-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYFRNRDID IERNSMNRSV QERKCRYSIR KLSVGAVSMI VGAVVFGTSP
60 70 80 90 100
VLAQEGASEQ PLANETQLSG ESSTLTDTEK SQPSSETELS GNKQEQERKD
110 120 130 140 150
KQEEKIPRDY YARDLENVET VIEKEDVETN ASNGQRVDLS SELDKLKKLE
160 170 180 190 200
NATVHMEFKP DAKAPAFYNL FSVSSATKKD EYFTMAVYNN TATLEGRGSD
210 220 230 240 250
GKQFYNNYND APLKVKPGQW NSVTFTVEKP TAELPKGRVR LYVNGVLSRT
260 270 280 290 300
SLRSGNFIKD MPDVTHVQIG ATKRANNTVW GSNLQIRNLT VYNRALTPEE
310 320 330 340 350
VQKRSQLFKR SDLEKKLPEG AALTEKTDIF ESGRNGKPNK DGIKSYRIPA
360 370 380 390 400
LLKTDKGTLI AGADERRLHS SDWGDIGMVI RRSEDNGKTW GDRVTITNLR
410 420 430 440 450
DNPKASDPSI GSPVNIDMVL VQDPETKRIF SIYDMFPEGK GIFGMSSQKE
460 470 480 490 500
EAYKKIDGKT YQILYREGEK GAYTIRENGT VYTPDGKATD YRVVVDPVKP
510 520 530 540 550
AYSDKGDLYK GNQLLGNIYF TTNKTSPFRI AKDSYLWMSY SDDDGKTWSA
560 570 580 590 600
PQDITPMVKA DWMKFLGVGP GTGIVLRNGP HKGRILIPVY TTNNVSHLNG
610 620 630 640 650
SQSSRIIYSD DHGKTWHAGE AVNDNRQVDG QKIHSSTMNN RRAQNTESTV
660 670 680 690 700
VQLNNGDVKL FMRGLTGDLQ VATSKDGGVT WEKDIKRYPQ VKDVYVQMSA
710 720 730 740 750
IHTMHEGKEY IILSNAGGPK RENGMVHLAR VEENGELTWL KHNPIQKGEF
760 770 780 790 800
AYNSLQELGN GEYGILYEHT EKGQNAYTLS FRKFNWDFLS KDLISPTEAK
810 820 830 840 850
VKRTREMGKG VIGLEFDSEV LVNKAPTLQL ANGKTARFMT QYDTKTLLFT
860 870 880 890 900
VDSEDMGQKV TGLAEGAIES MHNLPVSVAG TKLSNGMNGS EAAVHEVPEY
910 920 930 940 950
TGPLGTSGEE PAPTVEKPEY TGPLGTSGEE PAPTVEKPEY TGPLGTAGEE
960 970 980 990 1000
AAPTVEKPEF TGGVNGTEPA VHEIAEYKGS DSLVTLTTKE DYTYKAPLAQ
1010 1020 1030
QALPETGNKE SDLLASLGLT AFFLGLFTLG KKREQ
Length:1,035
Mass (Da):114,742
Last modified:July 19, 2004 - v1
Checksum:iC5B8A2D7A12E12F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1.
U43526 Genomic DNA. Translation: AAC44391.1.
PIRiT30287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1 .
U43526 Genomic DNA. Translation: AAC44391.1 .
PIRi T30287.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VVZ X-ray 2.50 A/B 319-822 [» ]
2YA4 X-ray 1.80 A/B 318-791 [» ]
2YA5 X-ray 2.00 A/B 318-791 [» ]
2YA6 X-ray 2.00 A/B 318-791 [» ]
2YA7 X-ray 1.89 A/B/C/D 318-791 [» ]
2YA8 X-ray 1.75 A/B 318-791 [» ]
4C1X X-ray 1.84 A 121-305 [» ]
ProteinModelPortali P62575.
SMRi P62575. Positions 322-791.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P62575.

Miscellaneous databases

EvolutionaryTracei P62575.

Family and domain databases

Gene3Di 2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR013320. ConA-like_dom.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR019931. LPXTG_anchor.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
Pfami PF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view ]
SMARTi SM00282. LamG. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A neuraminidase from Streptococcus pneumoniae has the features of a surface protein."
    Camara M., Boulnois G.J., Andrew P.W., Mitchell T.J.
    Infect. Immun. 62:3688-3695(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R36A / NCTC 10319.
  2. "Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
    Berry A.M., Lock R.A., Paton J.C.
    J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 882-1035.
    Strain: Serotype 6.

Entry informationi

Entry nameiNANA_STREE
AccessioniPrimary (citable) accession number: P62575
Secondary accession number(s): Q54722, Q59959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3