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P62575

- NANA_STREE

UniProt

P62575 - NANA_STREE

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Protein

Sialidase A

Gene
nanA
Organism
Streptococcus pneumoniae
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei347 – 3471Substrate By similarity
Active sitei372 – 3721Proton acceptor By similarity
Active sitei647 – 6471 By similarity
Binding sitei663 – 6631Substrate By similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP62575.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase A (EC:3.2.1.18)
Alternative name(s):
Neuraminidase A
Gene namesi
Name:nanA
OrganismiStreptococcus pneumoniae
Taxonomic identifieri1313 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

Secretedcell wall; Peptidoglycan-anchor Reviewed prediction

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
  3. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5353 Reviewed predictionAdd
BLAST
Chaini54 – 1006953Sialidase APRO_0000012034Add
BLAST
Propeptidei1007 – 103529Removed by sortase Reviewed predictionPRO_0000012035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1006 – 10061Pentaglycyl murein peptidoglycan amidated threonine Reviewed prediction

Keywords - PTMi

Peptidoglycan-anchor

Structurei

Secondary structure

1
1035
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi327 – 3304
Beta strandi345 – 3539
Beta strandi359 – 36810
Beta strandi370 – 38516
Beta strandi394 – 3974
Helixi408 – 4103
Beta strandi414 – 4229
Turni424 – 4263
Beta strandi429 – 4368
Beta strandi438 – 4403
Helixi441 – 4444
Beta strandi453 – 4564
Beta strandi459 – 4668
Beta strandi473 – 4753
Helixi477 – 4793
Beta strandi480 – 4823
Beta strandi488 – 4936
Helixi500 – 5023
Turni503 – 5064
Beta strandi507 – 5104
Beta strandi513 – 5175
Beta strandi525 – 5295
Beta strandi535 – 5439
Helixi555 – 5584
Beta strandi563 – 5686
Turni580 – 5834
Beta strandi585 – 5939
Turni594 – 5963
Helixi597 – 6004
Beta strandi603 – 6119
Turni622 – 6254
Beta strandi626 – 6283
Beta strandi631 – 6333
Turni635 – 6373
Helixi641 – 6433
Beta strandi647 – 6526
Beta strandi658 – 6625
Beta strandi665 – 68016
Beta strandi686 – 6927
Beta strandi699 – 7057
Beta strandi708 – 7169
Beta strandi718 – 73114
Beta strandi733 – 7353
Beta strandi737 – 74913
Beta strandi753 – 7597
Beta strandi762 – 7698
Beta strandi778 – 7858
Helixi786 – 7905

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VVZX-ray2.50A/B319-822[»]
2YA4X-ray1.80A/B318-791[»]
2YA5X-ray2.00A/B318-791[»]
2YA6X-ray2.00A/B318-791[»]
2YA7X-ray1.89A/B/C/D318-791[»]
2YA8X-ray1.75A/B318-791[»]
ProteinModelPortaliP62575.
SMRiP62575. Positions 322-791.

Miscellaneous databases

EvolutionaryTraceiP62575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati381 – 39212BNR 1Add
BLAST
Repeati539 – 55012BNR 2Add
BLAST
Repeati607 – 61812BNR 3Add
BLAST
Repeati672 – 68312BNR 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1003 – 10075LPXTG sorting signal Reviewed prediction

Sequence similaritiesi

Contains 4 BNR repeats.

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR019931. LPXTG_anchor.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00282. LamG. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62575-1 [UniParc]FASTAAdd to Basket

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MSYFRNRDID IERNSMNRSV QERKCRYSIR KLSVGAVSMI VGAVVFGTSP     50
VLAQEGASEQ PLANETQLSG ESSTLTDTEK SQPSSETELS GNKQEQERKD 100
KQEEKIPRDY YARDLENVET VIEKEDVETN ASNGQRVDLS SELDKLKKLE 150
NATVHMEFKP DAKAPAFYNL FSVSSATKKD EYFTMAVYNN TATLEGRGSD 200
GKQFYNNYND APLKVKPGQW NSVTFTVEKP TAELPKGRVR LYVNGVLSRT 250
SLRSGNFIKD MPDVTHVQIG ATKRANNTVW GSNLQIRNLT VYNRALTPEE 300
VQKRSQLFKR SDLEKKLPEG AALTEKTDIF ESGRNGKPNK DGIKSYRIPA 350
LLKTDKGTLI AGADERRLHS SDWGDIGMVI RRSEDNGKTW GDRVTITNLR 400
DNPKASDPSI GSPVNIDMVL VQDPETKRIF SIYDMFPEGK GIFGMSSQKE 450
EAYKKIDGKT YQILYREGEK GAYTIRENGT VYTPDGKATD YRVVVDPVKP 500
AYSDKGDLYK GNQLLGNIYF TTNKTSPFRI AKDSYLWMSY SDDDGKTWSA 550
PQDITPMVKA DWMKFLGVGP GTGIVLRNGP HKGRILIPVY TTNNVSHLNG 600
SQSSRIIYSD DHGKTWHAGE AVNDNRQVDG QKIHSSTMNN RRAQNTESTV 650
VQLNNGDVKL FMRGLTGDLQ VATSKDGGVT WEKDIKRYPQ VKDVYVQMSA 700
IHTMHEGKEY IILSNAGGPK RENGMVHLAR VEENGELTWL KHNPIQKGEF 750
AYNSLQELGN GEYGILYEHT EKGQNAYTLS FRKFNWDFLS KDLISPTEAK 800
VKRTREMGKG VIGLEFDSEV LVNKAPTLQL ANGKTARFMT QYDTKTLLFT 850
VDSEDMGQKV TGLAEGAIES MHNLPVSVAG TKLSNGMNGS EAAVHEVPEY 900
TGPLGTSGEE PAPTVEKPEY TGPLGTSGEE PAPTVEKPEY TGPLGTAGEE 950
AAPTVEKPEF TGGVNGTEPA VHEIAEYKGS DSLVTLTTKE DYTYKAPLAQ 1000
QALPETGNKE SDLLASLGLT AFFLGLFTLG KKREQ 1035
Length:1,035
Mass (Da):114,742
Last modified:July 19, 2004 - v1
Checksum:iC5B8A2D7A12E12F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1.
U43526 Genomic DNA. Translation: AAC44391.1.
PIRiT30287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72967 Genomic DNA. Translation: CAA51473.1 .
U43526 Genomic DNA. Translation: AAC44391.1 .
PIRi T30287.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VVZ X-ray 2.50 A/B 319-822 [» ]
2YA4 X-ray 1.80 A/B 318-791 [» ]
2YA5 X-ray 2.00 A/B 318-791 [» ]
2YA6 X-ray 2.00 A/B 318-791 [» ]
2YA7 X-ray 1.89 A/B/C/D 318-791 [» ]
2YA8 X-ray 1.75 A/B 318-791 [» ]
ProteinModelPortali P62575.
SMRi P62575. Positions 322-791.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P62575.

Miscellaneous databases

EvolutionaryTracei P62575.

Family and domain databases

Gene3Di 2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR004124. Glyco_hydro_33_N.
IPR001791. Laminin_G.
IPR019931. LPXTG_anchor.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR005877. YSIRK_signal_dom.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
Pfami PF02012. BNR. 1 hit.
PF02973. Sialidase. 1 hit.
PF04650. YSIRK_signal. 1 hit.
[Graphical view ]
SMARTi SM00282. LamG. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A neuraminidase from Streptococcus pneumoniae has the features of a surface protein."
    Camara M., Boulnois G.J., Andrew P.W., Mitchell T.J.
    Infect. Immun. 62:3688-3695(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: R36A / NCTC 10319.
  2. "Cloning and characterization of nanB, a second Streptococcus pneumoniae neuraminidase gene, and purification of the NanB enzyme from recombinant Escherichia coli."
    Berry A.M., Lock R.A., Paton J.C.
    J. Bacteriol. 178:4854-4860(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 882-1035.
    Strain: Serotype 6.

Entry informationi

Entry nameiNANA_STREE
AccessioniPrimary (citable) accession number: P62575
Secondary accession number(s): Q54722, Q59959
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi