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P62558

- SOPB_ECOLI

UniProt

P62558 - SOPB_ECOLI

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Protein

Protein SopB

Gene

sopB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Control of plasmid partitioning; required to recognize the cis-acting. Binds specifically with the DNA segment containing the sopC region. SopB is trans-acting.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi180 – 19920H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. plasmid partitioning Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Plasmid partition

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SopB
Alternative name(s):
Plasmid partition protein B
Gene namesi
Name:sopB
Synonyms:B
Ordered Locus Names:ECOK12F047
Encoded oniPlasmid F0 Publication
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Protein SopBPRO_0000068408Add
BLAST

Proteomic databases

PRIDEiP62558.

Expressioni

Gene expression databases

GenevestigatoriP62558.

Structurei

Secondary structure

1
323
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi160 – 17213Combined sources
Turni173 – 1775Combined sources
Helixi179 – 1868Combined sources
Helixi190 – 20112Combined sources
Helixi204 – 2085Combined sources
Beta strandi210 – 2123Combined sources
Helixi213 – 2153Combined sources
Helixi218 – 22710Combined sources
Turni228 – 2303Combined sources
Helixi232 – 24716Combined sources
Helixi254 – 26310Combined sources
Helixi267 – 2693Combined sources
Beta strandi278 – 2825Combined sources
Beta strandi285 – 2906Combined sources
Beta strandi293 – 2997Combined sources
Turni300 – 3023Combined sources
Helixi305 – 31814Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3KZ5X-ray1.58A/B/E276-323[»]
3MKWX-ray2.99B/P155-272[»]
3MKYX-ray2.86B/P155-323[»]
3MKZX-ray2.98A/B/N/U155-272[»]
ProteinModelPortaliP62558.
SMRiP62558. Positions 157-271, 276-322.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62558.

Family & Domainsi

Sequence similaritiesi

Belongs to the ParB family.Curated

Phylogenomic databases

OMAiRCINTAR.

Family and domain databases

InterProiIPR004437. ParB/RepB/Spo0J.
IPR003115. ParB/Sulfiredoxin_dom.
[Graphical view]
PfamiPF02195. ParBc. 1 hit.
[Graphical view]
SMARTiSM00470. ParB. 1 hit.
[Graphical view]
SUPFAMiSSF110849. SSF110849. 1 hit.
TIGRFAMsiTIGR00180. parB_part. 1 hit.

Sequencei

Sequence statusi: Complete.

P62558-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRAPVIPKH TLNTQPVEDT SLSTPAAPMV DSLIARVGVM ARGNAITLPV
60 70 80 90 100
CGRDVKFTLE VLRGDSVEKT SRVWSGNERD QELLTEDALD DLIPSFLLTG
110 120 130 140 150
QQTPAFGRRV SGVIEIADGS RRRKAAALTE SDYRVLVGEL DDEQMAALSR
160 170 180 190 200
LGNDYRPTSA YERGQRYASR LQNEFAGNIS ALADAENISR KIITRCINTA
210 220 230 240 250
KLPKSVVALF SHPGELSARS GDALQKAFTD KEELLKQQAS NLHEQKKAGV
260 270 280 290 300
IFEAEEVITL LTSVLKTSSA SRTSLSSRHQ FAPGATVLYK GDKMVLNLDR
310 320
SRVPTECIEK IEAILKELEK PAP
Length:323
Mass (Da):35,372
Last modified:July 19, 2004 - v1
Checksum:i3CC19C873CED3C1C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04619 Genomic DNA. Translation: CAA28296.1.
M12987 Genomic DNA. Translation: AAA24903.1.
AP001918 Genomic DNA. Translation: BAA97917.1.
PIRiT00244.
RefSeqiNP_061426.1. NC_002483.1.
YP_001294715.1. NC_009602.1.

Genome annotation databases

GeneIDi1263559.
5290921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X04619 Genomic DNA. Translation: CAA28296.1 .
M12987 Genomic DNA. Translation: AAA24903.1 .
AP001918 Genomic DNA. Translation: BAA97917.1 .
PIRi T00244.
RefSeqi NP_061426.1. NC_002483.1.
YP_001294715.1. NC_009602.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3KZ5 X-ray 1.58 A/B/E 276-323 [» ]
3MKW X-ray 2.99 B/P 155-272 [» ]
3MKY X-ray 2.86 B/P 155-323 [» ]
3MKZ X-ray 2.98 A/B/N/U 155-272 [» ]
ProteinModelPortali P62558.
SMRi P62558. Positions 157-271, 276-322.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P62558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1263559.
5290921.

Phylogenomic databases

OMAi RCINTAR.

Miscellaneous databases

EvolutionaryTracei P62558.

Gene expression databases

Genevestigatori P62558.

Family and domain databases

InterProi IPR004437. ParB/RepB/Spo0J.
IPR003115. ParB/Sulfiredoxin_dom.
[Graphical view ]
Pfami PF02195. ParBc. 1 hit.
[Graphical view ]
SMARTi SM00470. ParB. 1 hit.
[Graphical view ]
SUPFAMi SSF110849. SSF110849. 1 hit.
TIGRFAMsi TIGR00180. parB_part. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of the F plasmid genes essential for partitioning."
    Mori H., Kondo A., Ohshima A., Ogura T., Hiraga S.
    J. Mol. Biol. 192:1-15(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "F plasmid DNA complete mini-F region (F coordinates 40.301F to 49.869F)."
    Eichenlaub R.
    Submitted (AUG-1986) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes."
    Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / CR63.

Entry informationi

Entry nameiSOPB_ECOLI
AccessioniPrimary (citable) accession number: P62558
Secondary accession number(s): P08867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Overproduction of SopB protein causes IncG incompatibility.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3