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Protein

Glucans biosynthesis glucosyltransferase H

Gene

mdoH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).

Pathwayi: osmoregulated periplasmic glucan (OPG) biosynthesis

This protein is involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis and in Glycan metabolism.

GO - Molecular functioni

  • transferase activity, transferring glycosyl groups Source: EcoliWiki
  • transferase activity, transferring hexosyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  • beta-glucan biosynthetic process Source: EcoCyc
  • glucan biosynthetic process Source: EcoliWiki
  • response to osmotic stress Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG11886-MONOMER.
ECOL316407:JW1037-MONOMER.
UniPathwayiUPA00637.

Protein family/group databases

TCDBi4.D.3.1.1. the glycan glucosyl transferase (opgh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucans biosynthesis glucosyltransferase H (EC:2.4.1.-)
Gene namesi
Name:mdoH
Synonyms:opgH
Ordered Locus Names:b1049, JW1037
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11886. mdoH.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 139139CytoplasmicSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Topological domaini161 – 19333PeriplasmicSequence analysisAdd
BLAST
Transmembranei194 – 21421HelicalSequence analysisAdd
BLAST
Topological domaini215 – 512298CytoplasmicSequence analysisAdd
BLAST
Transmembranei513 – 53321HelicalSequence analysisAdd
BLAST
Topological domaini534 – 56936PeriplasmicSequence analysisAdd
BLAST
Transmembranei570 – 59021HelicalSequence analysisAdd
BLAST
Topological domaini591 – 60212CytoplasmicSequence analysisAdd
BLAST
Transmembranei603 – 62523HelicalSequence analysisAdd
BLAST
Topological domaini626 – 67954PeriplasmicSequence analysisAdd
BLAST
Transmembranei680 – 70021HelicalSequence analysisAdd
BLAST
Topological domaini701 – 847147CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 847847Glucans biosynthesis glucosyltransferase HPRO_0000210349Add
BLAST

Proteomic databases

EPDiP62517.
PaxDbiP62517.
PRIDEiP62517.

Interactioni

Protein-protein interaction databases

BioGridi4260685. 158 interactions.
IntActiP62517. 1 interaction.
STRINGi511145.b1049.

Structurei

3D structure databases

ProteinModelPortaliP62517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3A. Bacteria.
COG2943. LUCA.
HOGENOMiHOG000118994.
InParanoidiP62517.
KOiK03669.
OMAiQLYPLWP.
OrthoDBiEOG6TJ7V8.
PhylomeDBiP62517.

Family and domain databases

HAMAPiMF_01072. MdoH_OpgH.
InterProiIPR023725. Glucans_biosynth_gluTrFase_H.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P62517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTTEYIDA MPIAASEKAA LPKTDIRAVH QALDAEHRTW AREDDSPQGS
60 70 80 90 100
VKARLEQAWP DSLADGQLIK DDEGRDQLKA MPEAKRSSMF PDPWRTNPVG
110 120 130 140 150
RFWDRLRGRD VTPRYLARLT KEEQESEQKW RTVGTIRRYI LLILTLAQTV
160 170 180 190 200
VATWYMKTIL PYQGWALINP MDMVGQDLWV SFMQLLPYML QTGILILFAV
210 220 230 240 250
LFCWVSAGFW TALMGFLQLL IGRDKYSISA STVGDEPLNP EHRTALIMPI
260 270 280 290 300
CNEDVNRVFA GLRATWESVK ATGNAKHFDV YILSDSYNPD ICVAEQKAWM
310 320 330 340 350
ELIAEVGGEG QIFYRRRRRR VKRKSGNIDD FCRRWGSQYS YMVVLDADSV
360 370 380 390 400
MTGDCLCGLV RLMEANPNAG IIQSSPKASG MDTLYARCQQ FATRVYGPLF
410 420 430 440 450
TAGLHFWQLG ESHYWGHNAI IRVKPFIEHC ALAPLPGEGS FAGSILSHDF
460 470 480 490 500
VEAALMRRAG WGVWIAYDLP GSYEELPPNL LDELKRDRRW CHGNLMNFRL
510 520 530 540 550
FLVKGMHPVH RAVFLTGVMS YLSAPLWFMF LALSTALQVV HALTEPQYFL
560 570 580 590 600
QPRQLFPVWP QWRPELAIAL FASTMVLLFL PKLLSILLIW CKGTKEYGGF
610 620 630 640 650
WRVTLSLLLE VLFSVLLAPV RMLFHTVFVV SAFLGWEVVW NSPQRDDDST
660 670 680 690 700
SWGEAFKRHG SQLLLGLVWA VGMAWLDLRF LFWLAPIVFS LILSPFVSVI
710 720 730 740 750
SSRATVGLRT KRWKLFLIPE EYSPPQVLVD TDRFLEMNRQ RSLDDGFMHA
760 770 780 790 800
VFNPSFNALA TAMATARHRA SKVLEIARDR HVEQALNETP EKLNRDRRLV
810 820 830 840
LLSDPVTMAR LHFRVWNSPE RYSSWVSYYE GIKLNPLALR KPDAASQ
Length:847
Mass (Da):96,937
Last modified:July 19, 2004 - v1
Checksum:i7DCAF93640180944
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191A → G in CAA45522 (PubMed:7934824).Curated
Sequence conflicti289 – 2891P → L in CAA45522 (PubMed:7934824).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64197 Genomic DNA. Translation: CAA45522.1.
U00096 Genomic DNA. Translation: AAC74133.1.
AP009048 Genomic DNA. Translation: BAA35848.1.
PIRiF64847.
RefSeqiNP_415567.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74133; AAC74133; b1049.
BAA35848; BAA35848; BAA35848.
GeneIDi945624.
KEGGiecj:JW1037.
eco:b1049.
PATRICi32117337. VBIEscCol129921_1091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64197 Genomic DNA. Translation: CAA45522.1.
U00096 Genomic DNA. Translation: AAC74133.1.
AP009048 Genomic DNA. Translation: BAA35848.1.
PIRiF64847.
RefSeqiNP_415567.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP62517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260685. 158 interactions.
IntActiP62517. 1 interaction.
STRINGi511145.b1049.

Protein family/group databases

TCDBi4.D.3.1.1. the glycan glucosyl transferase (opgh) family.

Proteomic databases

EPDiP62517.
PaxDbiP62517.
PRIDEiP62517.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74133; AAC74133; b1049.
BAA35848; BAA35848; BAA35848.
GeneIDi945624.
KEGGiecj:JW1037.
eco:b1049.
PATRICi32117337. VBIEscCol129921_1091.

Organism-specific databases

EchoBASEiEB1832.
EcoGeneiEG11886. mdoH.

Phylogenomic databases

eggNOGiENOG4105C3A. Bacteria.
COG2943. LUCA.
HOGENOMiHOG000118994.
InParanoidiP62517.
KOiK03669.
OMAiQLYPLWP.
OrthoDBiEOG6TJ7V8.
PhylomeDBiP62517.

Enzyme and pathway databases

UniPathwayiUPA00637.
BioCyciEcoCyc:EG11886-MONOMER.
ECOL316407:JW1037-MONOMER.

Miscellaneous databases

PROiP62517.

Family and domain databases

HAMAPiMF_01072. MdoH_OpgH.
InterProiIPR023725. Glucans_biosynth_gluTrFase_H.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae."
    Loubens I., Debarbieux L., Bohin A., Lacroix J.-M., Bohin J.-P.
    Mol. Microbiol. 10:329-340(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Topological analysis of the membrane-bound glucosyltransferase, MdoH, required for osmoregulated periplasmic glucan synthesis in Escherichia coli."
    Debarbieux L., Bohin A., Bohin J.-P.
    J. Bacteriol. 179:6692-6698(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
    Strain: K12.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiOPGH_ECOLI
AccessioniPrimary (citable) accession number: P62517
Secondary accession number(s): P33137, P77371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: March 16, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.