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Protein

TSC22 domain family protein 1

Gene

Tsc22d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Acts on the C-type natriuretic peptide (CNP) promoter.

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
TSC22 domain family protein 1
Alternative name(s):
Regulatory protein TSC-22
TGFB-stimulated clone 22 homolog
TSC22-related inducible leucine zipper 1b
Transforming growth factor beta-1-induced transcript 4 protein
Gene namesi
Name:Tsc22d1
Synonyms:Kiaa1994, Tgfb1i4, Tilz1b, Tsc22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:109127. Tsc22d1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10771077TSC22 domain family protein 1PRO_0000219366Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei263 – 2631PhosphoserineBy similarity
Modified residuei1036 – 10361PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP62500.
PaxDbiP62500.
PRIDEiP62500.

PTM databases

iPTMnetiP62500.
PhosphoSiteiP62500.

Expressioni

Inductioni

By transforming growth factor beta-1 and other growth factors.

Gene expression databases

BgeeiP62500.
CleanExiMM_TSC22D1.
ExpressionAtlasiP62500. baseline and differential.
GenevisibleiP62500. MM.

Interactioni

Subunit structurei

Homodimer or heterodimer. Can form a heterodimer with TSC22D4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tsc22d4Q9EQN32EBI-8296837,EBI-7821198

Protein-protein interaction databases

BioGridi204159. 1 interaction.
IntActiP62500. 1 interaction.
STRINGi10090.ENSMUSP00000044517.

Structurei

3D structure databases

ProteinModelPortaliP62500.
SMRiP62500. Positions 992-1047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1010 – 103122Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 8224Pro-richAdd
BLAST
Compositional biasi195 – 24147His-richAdd
BLAST
Compositional biasi396 – 4049Poly-Gln
Compositional biasi464 – 49229Ser-richAdd
BLAST
Compositional biasi505 – 822318Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the TSC-22/Dip/Bun family.Curated

Phylogenomic databases

eggNOGiKOG4797. Eukaryota.
ENOG411251N. LUCA.
HOGENOMiHOG000090196.
HOVERGENiHBG108540.
InParanoidiP62500.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 2 hits.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62500-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHQPPESTAA AAAAADISAR KMAHPAMFPR RGSGGGSASA LNAAGTGVSG
60 70 80 90 100
AAPSSEDFPP PSLLQPPPPA ASSTQGPQPP PPQSLNLLSQ AQLQGQPLAP
110 120 130 140 150
GGTQMKKKSG FQITSVTPAQ ISASISSNNS IAEDTESYDD LDESHTEDLS
160 170 180 190 200
SSEILDVSLS RATDLGEPER SSSEETLNNF QEAETPGAVS PNQPHLPQPH
210 220 230 240 250
LPHLPQQNVV INGNAHPHHL HHHHHPHHGH HLHHGHHHSS HAAVAGPSIP
260 270 280 290 300
GGPPSSPVSR KLSTTGSSDG GVPVAPPPAV PSSGLPASVM TNIRTPSTTG
310 320 330 340 350
SLGINSVTGT SATNNVNIAA VGSFSPSVTN SVHGNANINT SNIPNAASIS
360 370 380 390 400
GGPGVTSVVN SSILSGMGNG TVSSSPVANS VLNAAAGITV GVVSSQQQQQ
410 420 430 440 450
QQQQPTVNTS RFRVVKLDST SEPFKKGRWT CTEFYEKENA VPATEGVAIN
460 470 480 490 500
KVVETVKQTP TEASSSERES TSGSSVSSSV STLSHYTESV GSGEMMGAPA
510 520 530 540 550
VVAPQQPPLP PAPPGLQGVA LQQLEFSSPA PQSIAAVSMP QSISQSQMSQ
560 570 580 590 600
VQLQPQELSF QQKQTLQPVP LQATMSAATG IQPSPVSVVG VTAAVGQQPS
610 620 630 640 650
VSSLAQPQLP YSQTAPPVQT PLPGAPPQQL QYGQQQPMVP AQIAPGHGQP
660 670 680 690 700
VTQNPTSEYV QQQQQPIFQA ALSSGQSSST GTGAGISVIP VAQAQGIQLP
710 720 730 740 750
GQPTAVQTQP AGAAGQPIGQ AQTAVSTVPT GGQIASIGQQ ANIPTAVQQP
760 770 780 790 800
STQVTPSVIQ QGAPPSSQVV LPAPTGIIHQ GVQTRASSLP QQLVIAPQST
810 820 830 840 850
LVTVPPQPQG VETVAQGVVS QQLPTGSPLP SASTISVTNQ VSSAAPSGMP
860 870 880 890 900
SVPTNLVPPQ NIAQPPATQN GSLVQSVSQS PLIATNINLP LAQQIPLSST
910 920 930 940 950
QFSTQSLAQA IGSQMEDARR PAEPSLGGLP QTMSGDSGGM SAVSDGSSSS
960 970 980 990 1000
LAAPASLFPL KVLPLTTPLV DGEDESSGAS VVAIDNKIEQ AMDLVKSHLM
1010 1020 1030 1040 1050
YAVREEVEVL KEQIKELIEK NSQLEQENNL LKTLASPEQL AQFQAQLQTG
1060 1070
SPPATTQPQG TTQPPAQPAS QGSGSTA
Length:1,077
Mass (Da):109,811
Last modified:September 2, 2008 - v2
Checksum:i35749C0C1CA1E1B6
GO
Isoform 2 (identifier: P62500-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-934: Missing.
     935-975: GDSGGMSAVS...TTPLVDGEDE → MKSQWCRPVA...TENASVRLDN

Show »
Length:143
Mass (Da):15,583
Checksum:i729D4688572496F0
GO
Isoform 3 (identifier: P62500-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-384: Missing.

Note: No experimental confirmation available.
Show »
Length:995
Mass (Da):102,086
Checksum:i7EA26AE92A87B0E9
GO

Sequence cautioni

The sequence AAG41218.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH58660.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD32600.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3951S → SQ in AAG41218 (Ref. 4) Curated
Sequence conflicti396 – 3983Missing in BAD32600 (PubMed:15368895).Curated
Sequence conflicti449 – 4491I → V in AAG41218 (Ref. 4) Curated
Sequence conflicti449 – 4491I → V in AAH58660 (PubMed:15489334).Curated
Sequence conflicti551 – 5511V → L in AAG41218 (Ref. 4) Curated
Sequence conflicti591 – 5911V → I in AAG41218 (Ref. 4) Curated
Sequence conflicti591 – 5911V → I in AAH58660 (PubMed:15489334).Curated
Sequence conflicti660 – 6601V → VQQ in BAD32600 (PubMed:15368895).Curated
Sequence conflicti714 – 7141A → P in BAD32600 (PubMed:15368895).Curated
Sequence conflicti720 – 7201Q → P in AAG41218 (Ref. 4) Curated
Sequence conflicti737 – 7371I → T in AAG41218 (Ref. 4) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 934934Missing in isoform 2. 1 PublicationVSP_035327Add
BLAST
Alternative sequencei303 – 38482Missing in isoform 3. 1 PublicationVSP_035328Add
BLAST
Alternative sequencei935 – 97541GDSGG…DGEDE → MKSQWCRPVAMDLGVYQLRH FSISFLSSLLGTENASVRLD N in isoform 2. 1 PublicationVSP_035329Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62940 mRNA. Translation: CAA44712.1.
AK173322 mRNA. Translation: BAD32600.1. Different initiation.
BC058660 mRNA. Translation: AAH58660.1. Different initiation.
AF201285 mRNA. Translation: AAG41218.1. Different initiation.
CCDSiCCDS27285.2. [P62500-1]
CCDS27286.1. [P62500-2]
CCDS79344.1. [P62500-3]
PIRiS23255.
RefSeqiNP_033392.1. NM_009366.4. [P62500-2]
NP_997535.2. NM_207652.3.
UniGeneiMm.153272.

Genome annotation databases

EnsembliENSMUST00000022587; ENSMUSP00000022587; ENSMUSG00000022010. [P62500-2]
GeneIDi21807.
KEGGimmu:21807.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62940 mRNA. Translation: CAA44712.1.
AK173322 mRNA. Translation: BAD32600.1. Different initiation.
BC058660 mRNA. Translation: AAH58660.1. Different initiation.
AF201285 mRNA. Translation: AAG41218.1. Different initiation.
CCDSiCCDS27285.2. [P62500-1]
CCDS27286.1. [P62500-2]
CCDS79344.1. [P62500-3]
PIRiS23255.
RefSeqiNP_033392.1. NM_009366.4. [P62500-2]
NP_997535.2. NM_207652.3.
UniGeneiMm.153272.

3D structure databases

ProteinModelPortaliP62500.
SMRiP62500. Positions 992-1047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204159. 1 interaction.
IntActiP62500. 1 interaction.
STRINGi10090.ENSMUSP00000044517.

PTM databases

iPTMnetiP62500.
PhosphoSiteiP62500.

Proteomic databases

MaxQBiP62500.
PaxDbiP62500.
PRIDEiP62500.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022587; ENSMUSP00000022587; ENSMUSG00000022010. [P62500-2]
GeneIDi21807.
KEGGimmu:21807.

Organism-specific databases

CTDi8848.
MGIiMGI:109127. Tsc22d1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4797. Eukaryota.
ENOG411251N. LUCA.
HOGENOMiHOG000090196.
HOVERGENiHBG108540.
InParanoidiP62500.

Miscellaneous databases

ChiTaRSiTsc22d1. mouse.
PROiP62500.
SOURCEiSearch...

Gene expression databases

BgeeiP62500.
CleanExiMM_TSC22D1.
ExpressionAtlasiP62500. baseline and differential.
GenevisibleiP62500. MM.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 2 hits.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta 1 and other growth factors."
    Shibanuma M., Kuroki T., Nose K.
    J. Biol. Chem. 267:10219-10224(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Osteoblast.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Embryonic tail.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Identification and characterization of a family of leucine zipper genes related to TSC22."
    Ershler M.A., Belyavsky A.V., Visser J.W.M.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-1077 (ISOFORM 1).
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Testis.

Entry informationi

Entry nameiT22D1_MOUSE
AccessioniPrimary (citable) accession number: P62500
Secondary accession number(s): Q00992
, Q69Z44, Q6PDK1, Q9EQN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: September 2, 2008
Last modified: June 8, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.