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Protein

Eukaryotic peptide chain release factor subunit 1

Gene

ETF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • ribosome binding Source: UniProtKB
  • RNA binding Source: ProtInc
  • translation release factor activity Source: UniProtKB
  • translation release factor activity, codon specific Source: InterPro
  • translation termination factor activity Source: ProtInc

GO - Biological processi

  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  • protein methylation Source: MGI
  • regulation of translational termination Source: ProtInc
  • translational termination Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Nonsense-mediated mRNA decay, Protein biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120705-MONOMER.
ReactomeiR-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1
Short name:
Eukaryotic release factor 1
Short name:
eRF1
Alternative name(s):
Protein Cl1
TB3-1
Gene namesi
Name:ETF1
Synonyms:ERF1, RF1, SUP45L1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3477. ETF1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi2107.
OpenTargetsiENSG00000120705.
PharmGKBiPA27893.

Polymorphism and mutation databases

BioMutaiETF1.
DMDMi50402099.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001431382 – 437Eukaryotic peptide chain release factor subunit 1Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei347PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62495.
MaxQBiP62495.
PaxDbiP62495.
PeptideAtlasiP62495.
PRIDEiP62495.

PTM databases

iPTMnetiP62495.
PhosphoSitePlusiP62495.
SwissPalmiP62495.

Expressioni

Gene expression databases

BgeeiENSG00000120705.
CleanExiHS_ETF1.
ExpressionAtlasiP62495. baseline and differential.
GenevisibleiP62495. HS.

Organism-specific databases

HPAiCAB011686.
HPA037511.

Interactioni

Subunit structurei

Heterodimer of two subunits, one of which binds GTP. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GSPT1P151702EBI-750990,EBI-948993
KEAP1Q141457EBI-750990,EBI-751001

Protein-protein interaction databases

BioGridi108408. 50 interactors.
IntActiP62495. 26 interactors.
MINTiMINT-5004401.
STRINGi9606.ENSP00000353741.

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Turni7 – 9Combined sources3
Helixi10 – 25Combined sources16
Beta strandi30 – 32Combined sources3
Beta strandi34 – 39Combined sources6
Beta strandi41 – 43Combined sources3
Helixi45 – 59Combined sources15
Beta strandi62 – 64Combined sources3
Helixi66 – 82Combined sources17
Beta strandi93 – 101Combined sources9
Helixi103 – 105Combined sources3
Beta strandi107 – 114Combined sources8
Beta strandi124 – 130Combined sources7
Helixi134 – 139Combined sources6
Beta strandi145 – 150Combined sources6
Beta strandi158 – 162Combined sources5
Beta strandi165 – 170Combined sources6
Beta strandi180 – 185Combined sources6
Helixi188 – 197Combined sources10
Turni198 – 203Combined sources6
Helixi204 – 208Combined sources5
Turni209 – 212Combined sources4
Beta strandi216 – 218Combined sources3
Beta strandi220 – 222Combined sources3
Beta strandi225 – 229Combined sources5
Turni230 – 233Combined sources4
Helixi234 – 237Combined sources4
Beta strandi240 – 242Combined sources3
Turni244 – 249Combined sources6
Beta strandi253 – 255Combined sources3
Helixi262 – 271Combined sources10
Turni274 – 276Combined sources3
Helixi278 – 295Combined sources18
Beta strandi296 – 298Combined sources3
Beta strandi301 – 304Combined sources4
Helixi305 – 313Combined sources9
Beta strandi318 – 324Combined sources7
Beta strandi329 – 333Combined sources5
Beta strandi336 – 339Combined sources4
Beta strandi342 – 346Combined sources5
Helixi348 – 352Combined sources5
Turni354 – 356Combined sources3
Turni360 – 362Combined sources3
Beta strandi368 – 372Combined sources5
Helixi374 – 380Combined sources7
Turni383 – 386Combined sources4
Beta strandi389 – 392Combined sources4
Beta strandi394 – 396Combined sources3
Helixi397 – 404Combined sources8
Turni405 – 408Combined sources4
Beta strandi409 – 412Combined sources4
Beta strandi420 – 422Combined sources3
Turni423 – 425Combined sources3
Beta strandi426 – 428Combined sources3
Helixi434 – 437Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DT9X-ray2.70A1-437[»]
2HSTNMR-A140-275[»]
2KTUNMR-A276-437[»]
2KTVNMR-A276-437[»]
2LGTNMR-A1-142[»]
2LLXNMR-A1-142[»]
2MQ6NMR-A1-142[»]
2MQ9NMR-A1-142[»]
3E1YX-ray3.80A/B/C/D1-437[»]
3J5Yelectron microscopy9.70A7-420[»]
3JAGelectron microscopy3.65ii6-421[»]
3JAHelectron microscopy3.45ii6-421[»]
3JAIelectron microscopy3.65ii6-421[»]
4D5Nelectron microscopy9.00A5-437[»]
4D61electron microscopy9.00h5-437[»]
5A8Lelectron microscopy3.80Q7-437[»]
DisProtiDP00310.
ProteinModelPortaliP62495.
SMRiP62495.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62495.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

eggNOGiKOG0688. Eukaryota.
COG1503. LUCA.
GeneTreeiENSGT00390000009004.
HOGENOMiHOG000224681.
HOVERGENiHBG005602.
InParanoidiP62495.
KOiK03265.
OMAiYRCDSEF.
OrthoDBiEOG091G0MEJ.
PhylomeDBiP62495.
TreeFamiTF105672.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.30.960.10. 1 hit.
InterProiIPR005140. eRF1_1_Pelota.
IPR005141. eRF1_2.
IPR005142. eRF1_3.
IPR029064. L30e-like.
IPR004403. Peptide_chain-rel_eRF1/aRF1.
IPR024049. Release_factor_eRF1/aRF1_N.
[Graphical view]
PANTHERiPTHR10113. PTHR10113. 1 hit.
PfamiPF03463. eRF1_1. 1 hit.
PF03464. eRF1_2. 1 hit.
PF03465. eRF1_3. 1 hit.
[Graphical view]
SMARTiSM01194. eRF1_1. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF55481. SSF55481. 1 hit.
TIGRFAMsiTIGR03676. aRF1/eRF1. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62495-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK
60 70 80 90 100
MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI
110 120 130 140 150
VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDDSKFGFIV
160 170 180 190 200
IDGSGALFGT LQGNTREVLH KFTVDLPKKH GRGGQSALRF ARLRMEKRHN
210 220 230 240 250
YVRKVAETAV QLFISGDKVN VAGLVLAGSA DFKTELSQSD MFDQRLQSKV
260 270 280 290 300
LKLVDISYGG ENGFNQAIEL STEVLSNVKF IQEKKLIGRY FDEISQDTGK
310 320 330 340 350
YCFGVEDTLK ALEMGAVEIL IVYENLDIMR YVLHCQGTEE EKILYLTPEQ
360 370 380 390 400
EKDKSHFTDK ETGQEHELIE SMPLLEWFAN NYKKFGATLE IVTDKSQEGS
410 420 430
QFVKGFGGIG GILRYRVDFQ GMEYQGGDDE FFDLDDY
Length:437
Mass (Da):49,031
Last modified:January 23, 2007 - v3
Checksum:iCECC50D100E59D19
GO
Isoform 2 (identifier: P62495-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Show »
Length:404
Mass (Da):45,463
Checksum:iF70FB4B7D81FD5C1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561891 – 33Missing in isoform 2. 2 PublicationsAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75715 mRNA. Translation: AAA36665.1. Sequence problems.
X81625 mRNA. Translation: CAA57281.1.
U90176 mRNA. Translation: AAB49726.1.
AF095901 Genomic DNA. Translation: AAD43966.1.
BT007374 mRNA. Translation: AAP36038.1.
AK312510 mRNA. Translation: BAG35411.1.
AC011385 Genomic DNA. No translation available.
AC113403 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62130.1.
CH471062 Genomic DNA. Translation: EAW62131.1.
CH471062 Genomic DNA. Translation: EAW62132.1.
BC088358 mRNA. Translation: AAH88358.1.
BC014269 mRNA. Translation: AAH14269.1.
CCDSiCCDS4207.1. [P62495-1]
CCDS75313.1. [P62495-2]
PIRiS50853.
RefSeqiNP_001243231.1. NM_001256302.1. [P62495-2]
NP_001278903.1. NM_001291974.1. [P62495-2]
NP_001278904.1. NM_001291975.1. [P62495-2]
NP_004721.1. NM_004730.3. [P62495-1]
XP_005271978.1. XM_005271921.1. [P62495-2]
UniGeneiHs.483494.

Genome annotation databases

EnsembliENST00000360541; ENSP00000353741; ENSG00000120705. [P62495-1]
ENST00000499810; ENSP00000421288; ENSG00000120705. [P62495-2]
GeneIDi2107.
KEGGihsa:2107.
UCSCiuc003ldc.6. human. [P62495-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75715 mRNA. Translation: AAA36665.1. Sequence problems.
X81625 mRNA. Translation: CAA57281.1.
U90176 mRNA. Translation: AAB49726.1.
AF095901 Genomic DNA. Translation: AAD43966.1.
BT007374 mRNA. Translation: AAP36038.1.
AK312510 mRNA. Translation: BAG35411.1.
AC011385 Genomic DNA. No translation available.
AC113403 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62130.1.
CH471062 Genomic DNA. Translation: EAW62131.1.
CH471062 Genomic DNA. Translation: EAW62132.1.
BC088358 mRNA. Translation: AAH88358.1.
BC014269 mRNA. Translation: AAH14269.1.
CCDSiCCDS4207.1. [P62495-1]
CCDS75313.1. [P62495-2]
PIRiS50853.
RefSeqiNP_001243231.1. NM_001256302.1. [P62495-2]
NP_001278903.1. NM_001291974.1. [P62495-2]
NP_001278904.1. NM_001291975.1. [P62495-2]
NP_004721.1. NM_004730.3. [P62495-1]
XP_005271978.1. XM_005271921.1. [P62495-2]
UniGeneiHs.483494.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DT9X-ray2.70A1-437[»]
2HSTNMR-A140-275[»]
2KTUNMR-A276-437[»]
2KTVNMR-A276-437[»]
2LGTNMR-A1-142[»]
2LLXNMR-A1-142[»]
2MQ6NMR-A1-142[»]
2MQ9NMR-A1-142[»]
3E1YX-ray3.80A/B/C/D1-437[»]
3J5Yelectron microscopy9.70A7-420[»]
3JAGelectron microscopy3.65ii6-421[»]
3JAHelectron microscopy3.45ii6-421[»]
3JAIelectron microscopy3.65ii6-421[»]
4D5Nelectron microscopy9.00A5-437[»]
4D61electron microscopy9.00h5-437[»]
5A8Lelectron microscopy3.80Q7-437[»]
DisProtiDP00310.
ProteinModelPortaliP62495.
SMRiP62495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108408. 50 interactors.
IntActiP62495. 26 interactors.
MINTiMINT-5004401.
STRINGi9606.ENSP00000353741.

PTM databases

iPTMnetiP62495.
PhosphoSitePlusiP62495.
SwissPalmiP62495.

Polymorphism and mutation databases

BioMutaiETF1.
DMDMi50402099.

Proteomic databases

EPDiP62495.
MaxQBiP62495.
PaxDbiP62495.
PeptideAtlasiP62495.
PRIDEiP62495.

Protocols and materials databases

DNASUi2107.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360541; ENSP00000353741; ENSG00000120705. [P62495-1]
ENST00000499810; ENSP00000421288; ENSG00000120705. [P62495-2]
GeneIDi2107.
KEGGihsa:2107.
UCSCiuc003ldc.6. human. [P62495-1]

Organism-specific databases

CTDi2107.
DisGeNETi2107.
GeneCardsiETF1.
HGNCiHGNC:3477. ETF1.
HPAiCAB011686.
HPA037511.
MIMi600285. gene.
neXtProtiNX_P62495.
OpenTargetsiENSG00000120705.
PharmGKBiPA27893.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0688. Eukaryota.
COG1503. LUCA.
GeneTreeiENSGT00390000009004.
HOGENOMiHOG000224681.
HOVERGENiHBG005602.
InParanoidiP62495.
KOiK03265.
OMAiYRCDSEF.
OrthoDBiEOG091G0MEJ.
PhylomeDBiP62495.
TreeFamiTF105672.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120705-MONOMER.
ReactomeiR-HSA-72764. Eukaryotic Translation Termination.
R-HSA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-HSA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

ChiTaRSiETF1. human.
EvolutionaryTraceiP62495.
GeneWikiiEukaryotic_release_factors.
GenomeRNAii2107.
PROiP62495.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120705.
CleanExiHS_ETF1.
ExpressionAtlasiP62495. baseline and differential.
GenevisibleiP62495. HS.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.30.960.10. 1 hit.
InterProiIPR005140. eRF1_1_Pelota.
IPR005141. eRF1_2.
IPR005142. eRF1_3.
IPR029064. L30e-like.
IPR004403. Peptide_chain-rel_eRF1/aRF1.
IPR024049. Release_factor_eRF1/aRF1_N.
[Graphical view]
PANTHERiPTHR10113. PTHR10113. 1 hit.
PfamiPF03463. eRF1_1. 1 hit.
PF03464. eRF1_2. 1 hit.
PF03465. eRF1_3. 1 hit.
[Graphical view]
SMARTiSM01194. eRF1_1. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF55481. SSF55481. 1 hit.
TIGRFAMsiTIGR03676. aRF1/eRF1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiERF1_HUMAN
AccessioniPrimary (citable) accession number: P62495
Secondary accession number(s): B2R6B4
, D3DQC1, P46055, Q5M7Z7, Q96CG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.