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Protein

Ras-related protein Rab-11A

Gene

RAB11A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269GTP3 Publications
Nucleotide bindingi66 – 705GTP3 Publications
Nucleotide bindingi124 – 1274GTP3 Publications
Nucleotide bindingi154 – 1563GTP3 Publications

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • myosin V binding Source: UniProtKB
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • astral microtubule organization Source: UniProtKB
  • cytokinesis Source: UniProtKB
  • establishment of protein localization to membrane Source: UniProtKB
  • establishment of protein localization to organelle Source: UniProtKB
  • establishment of vesicle localization Source: UniProtKB
  • exosomal secretion Source: UniProtKB
  • melanosome transport Source: UniProtKB
  • mitotic metaphase plate congression Source: UniProtKB
  • mitotic spindle assembly Source: UniProtKB
  • multivesicular body assembly Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • plasma membrane to endosome transport Source: UniProtKB
  • positive regulation of axon extension Source: Ensembl
  • positive regulation of epithelial cell migration Source: UniProtKB
  • positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of long-term neuronal synaptic plasticity Source: Ensembl
  • regulation of multivesicular body size Source: UniProtKB
  • regulation of protein transport Source: Ensembl
  • regulation of vesicle-mediated transport Source: UniProtKB
  • renal water homeostasis Source: Reactome
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5620916. VxPx cargo-targeting to cilium.
SignaLinkiP62491.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-11A
Short name:
Rab-11
Alternative name(s):
YL8
Gene namesi
Name:RAB11A
Synonyms:RAB11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:9760. RAB11A.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • cleavage furrow Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • mitochondrion Source: Ensembl
  • multivesicular body Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
  • phagocytic vesicle Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB-SubCell
  • spindle pole Source: UniProtKB
  • trans-Golgi network Source: MGI
  • transport vesicle Source: Ensembl
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251S → N: Dominant-negative mutant. Induces increased number of binucleated cells, indicating defects in cytokinesis. Inhibits the transport of NPC1L1 to the plama membrane. Disrupts the trafficking of CDH1 to the plasma membrane and promotes accumulation of CDH1 in RAB11A endosomes in nonpolarized cells. Promotes mistargeting of CDH1 to the apical membrane in polarized cells. 3 Publications
Mutagenesisi70 – 701Q → L: Constitutively active mutant. Decreases GTPase activity. Disrupts the trafficking of CDH1 to the plasma membrane and promotes accumulation of CDH1 in RAB11A endosomes in nonpolarized cells. Promotes mistargeting of CDH1 to the apical membrane in polarized cells. 5 Publications

Organism-specific databases

PharmGKBiPA34101.

Polymorphism and mutation databases

BioMutaiRAB11A.
DMDMi50402542.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 213212Ras-related protein Rab-11APRO_0000121151Add
BLAST
Propeptidei214 – 2163Removed in mature formSequence analysisPRO_0000370807

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine1 Publication
Lipidationi212 – 2121S-geranylgeranyl cysteine1 Publication
Modified residuei213 – 2131Cysteine methyl esterSequence analysis
Lipidationi213 – 2131S-geranylgeranyl cysteine1 Publication

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP62491.
PaxDbiP62491.
PeptideAtlasiP62491.
PRIDEiP62491.
TopDownProteomicsiP62491-1. [P62491-1]

2D gel databases

OGPiP62491.

PTM databases

iPTMnetiP62491.
PhosphoSiteiP62491.
SwissPalmiP62491.

Expressioni

Gene expression databases

BgeeiENSG00000103769.
CleanExiHS_RAB11A.
ExpressionAtlasiP62491. baseline and differential.
GenevisibleiP62491. HS.

Organism-specific databases

HPAiCAB013097.
HPA051697.
HPA060686.

Interactioni

Subunit structurei

Interacts with RIP11 and STXBP6. Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). Interacts with EXOC6 in a GTP-dependent manner (By similarity). Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 (via its C-terminus) and RAB11FIP4. Interacts with EVI5; EVI5 and RAB11FIP3 may be mutually exclusive and compete for binding RAB11A. Interacts with VIPAS39. Interacts with MYO5B. Found in a complex with MYO5B and CFTR. Interacts with NPC1L1. Interacts (GDP-bound form) with ZFYVE27. Interacts with BIRC6/bruce. May interact with TBC1D14. Interacts with UNC119; in a cell cycle-dependent manner. GDP-bound and nucleotide-free forms interact with SH3BP5 (PubMed:26506309).By similarity23 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CHMP1BQ7LBR13EBI-745098,EBI-2118090
RAB11FIP2Q7L8043EBI-745098,EBI-1049676
RAB11FIP5Q9BXF62EBI-745098,EBI-1387068
ZFYVE27Q5T4F44EBI-745098,EBI-3892947

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • myosin V binding Source: UniProtKB
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114299. 89 interactions.
DIPiDIP-29138N.
IntActiP62491. 40 interactions.
MINTiMINT-1434585.
STRINGi9606.ENSP00000261890.

Structurei

Secondary structure

1
216
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 1810Combined sources
Helixi24 – 3310Combined sources
Beta strandi45 – 5511Combined sources
Beta strandi58 – 6710Combined sources
Beta strandi72 – 743Combined sources
Helixi78 – 814Combined sources
Beta strandi86 – 927Combined sources
Helixi96 – 1005Combined sources
Helixi102 – 11211Combined sources
Beta strandi118 – 1247Combined sources
Helixi126 – 1316Combined sources
Helixi136 – 14510Combined sources
Beta strandi149 – 1524Combined sources
Turni155 – 1573Combined sources
Helixi161 – 17212Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OIVX-ray1.98A/B1-173[»]
1OIWX-ray2.05A1-173[»]
1OIXX-ray1.70A1-173[»]
1YZKX-ray2.00A8-175[»]
2D7CX-ray1.75A/B7-173[»]
2GZDX-ray2.44A/B2-173[»]
2GZHX-ray2.47A2-173[»]
2HV8X-ray1.86A/B/C6-175[»]
4C4PX-ray2.00A1-173[»]
4D0LX-ray2.94B/D/F1-216[»]
4D0MX-ray6.00B/D/H/J/N/P/R/T/X/Z/d/h1-216[»]
4LWZX-ray2.55A/C1-177[»]
4LX0X-ray2.19A/C1-177[»]
4UJ3X-ray3.00A/D/G/J/M/P/S/V4-186[»]
4UJ4X-ray4.20A/D/G/J4-186[»]
4UJ5X-ray2.60A/B6-186[»]
5C46X-ray2.65F1-216[»]
5C4GX-ray3.20B1-216[»]
5EUQX-ray3.20B1-216[»]
ProteinModelPortaliP62491.
SMRiP62491. Positions 6-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62491.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 489Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP62491.
KOiK07904.
OMAiAMGTRDD.
OrthoDBiEOG091G0O1R.
PhylomeDBiP62491.
TreeFamiTF300099.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62491-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTRDDEYDY LFKVVLIGDS GVGKSNLLSR FTRNEFNLES KSTIGVEFAT
60 70 80 90 100
RSIQVDGKTI KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHLTYE
110 120 130 140 150
NVERWLKELR DHADSNIVIM LVGNKSDLRH LRAVPTDEAR AFAEKNGLSF
160 170 180 190 200
IETSALDSTN VEAAFQTILT EIYRIVSQKQ MSDRRENDMS PSNNVVPIHV
210
PPTTENKPKV QCCQNI
Length:216
Mass (Da):24,394
Last modified:January 23, 2007 - v3
Checksum:i76FC1E113A29B269
GO
Isoform 2 (identifier: P62491-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     147-216: GLSFIETSAL...KPKVQCCQNI → EANVRQTRK

Note: No experimental confirmation available.
Show »
Length:155
Mass (Da):17,659
Checksum:i70C3A0F378C9893A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei147 – 21670GLSFI…CCQNI → EANVRQTRK in isoform 2. 1 PublicationVSP_046755Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53143 mRNA. Translation: CAA37300.1.
X56740 mRNA. Translation: CAA40064.1.
AF000231 mRNA. Translation: AAC32887.1.
AF498946 mRNA. Translation: AAM21094.1.
CR407669 mRNA. Translation: CAG28597.1.
CR536493 mRNA. Translation: CAG38732.1.
BT020151 mRNA. Translation: AAV38953.1.
BT020154 mRNA. Translation: AAV38956.1.
AK300008 mRNA. Translation: BAG61825.1.
AK311770 mRNA. Translation: BAG34713.1.
AC011939 Genomic DNA. No translation available.
AC084854 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77752.1.
BC013348 mRNA. Translation: AAH13348.1.
CCDSiCCDS10212.1. [P62491-1]
CCDS58373.1. [P62491-2]
PIRiS47169.
RefSeqiNP_001193765.1. NM_001206836.1. [P62491-2]
NP_004654.1. NM_004663.4. [P62491-1]
UniGeneiHs.321541.

Genome annotation databases

EnsembliENST00000261890; ENSP00000261890; ENSG00000103769. [P62491-1]
ENST00000569896; ENSP00000456420; ENSG00000103769. [P62491-2]
GeneIDi8766.
KEGGihsa:8766.
UCSCiuc002apk.4. human. [P62491-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53143 mRNA. Translation: CAA37300.1.
X56740 mRNA. Translation: CAA40064.1.
AF000231 mRNA. Translation: AAC32887.1.
AF498946 mRNA. Translation: AAM21094.1.
CR407669 mRNA. Translation: CAG28597.1.
CR536493 mRNA. Translation: CAG38732.1.
BT020151 mRNA. Translation: AAV38953.1.
BT020154 mRNA. Translation: AAV38956.1.
AK300008 mRNA. Translation: BAG61825.1.
AK311770 mRNA. Translation: BAG34713.1.
AC011939 Genomic DNA. No translation available.
AC084854 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77752.1.
BC013348 mRNA. Translation: AAH13348.1.
CCDSiCCDS10212.1. [P62491-1]
CCDS58373.1. [P62491-2]
PIRiS47169.
RefSeqiNP_001193765.1. NM_001206836.1. [P62491-2]
NP_004654.1. NM_004663.4. [P62491-1]
UniGeneiHs.321541.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OIVX-ray1.98A/B1-173[»]
1OIWX-ray2.05A1-173[»]
1OIXX-ray1.70A1-173[»]
1YZKX-ray2.00A8-175[»]
2D7CX-ray1.75A/B7-173[»]
2GZDX-ray2.44A/B2-173[»]
2GZHX-ray2.47A2-173[»]
2HV8X-ray1.86A/B/C6-175[»]
4C4PX-ray2.00A1-173[»]
4D0LX-ray2.94B/D/F1-216[»]
4D0MX-ray6.00B/D/H/J/N/P/R/T/X/Z/d/h1-216[»]
4LWZX-ray2.55A/C1-177[»]
4LX0X-ray2.19A/C1-177[»]
4UJ3X-ray3.00A/D/G/J/M/P/S/V4-186[»]
4UJ4X-ray4.20A/D/G/J4-186[»]
4UJ5X-ray2.60A/B6-186[»]
5C46X-ray2.65F1-216[»]
5C4GX-ray3.20B1-216[»]
5EUQX-ray3.20B1-216[»]
ProteinModelPortaliP62491.
SMRiP62491. Positions 6-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114299. 89 interactions.
DIPiDIP-29138N.
IntActiP62491. 40 interactions.
MINTiMINT-1434585.
STRINGi9606.ENSP00000261890.

PTM databases

iPTMnetiP62491.
PhosphoSiteiP62491.
SwissPalmiP62491.

Polymorphism and mutation databases

BioMutaiRAB11A.
DMDMi50402542.

2D gel databases

OGPiP62491.

Proteomic databases

MaxQBiP62491.
PaxDbiP62491.
PeptideAtlasiP62491.
PRIDEiP62491.
TopDownProteomicsiP62491-1. [P62491-1]

Protocols and materials databases

DNASUi8766.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261890; ENSP00000261890; ENSG00000103769. [P62491-1]
ENST00000569896; ENSP00000456420; ENSG00000103769. [P62491-2]
GeneIDi8766.
KEGGihsa:8766.
UCSCiuc002apk.4. human. [P62491-1]

Organism-specific databases

CTDi8766.
GeneCardsiRAB11A.
HGNCiHGNC:9760. RAB11A.
HPAiCAB013097.
HPA051697.
HPA060686.
MIMi605570. gene.
neXtProtiNX_P62491.
PharmGKBiPA34101.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP62491.
KOiK07904.
OMAiAMGTRDD.
OrthoDBiEOG091G0O1R.
PhylomeDBiP62491.
TreeFamiTF300099.

Enzyme and pathway databases

ReactomeiR-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5620916. VxPx cargo-targeting to cilium.
SignaLinkiP62491.

Miscellaneous databases

ChiTaRSiRAB11A. human.
EvolutionaryTraceiP62491.
GeneWikiiRAB11A.
GenomeRNAii8766.
PROiP62491.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103769.
CleanExiHS_RAB11A.
ExpressionAtlasiP62491. baseline and differential.
GenevisibleiP62491. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB11A_HUMAN
AccessioniPrimary (citable) accession number: P62491
Secondary accession number(s): B2R4B6
, B4DT13, P24410, Q5TZN9, Q9JLX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.