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Protein

DNA-directed RNA polymerase II subunit RPB7

Gene

Polr2g

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiREACT_271824. RNA Polymerase II Promoter Escape.
REACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_319326. mRNA Splicing - Minor Pathway.
REACT_319445. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_323062. mRNA Capping.
REACT_323975. Dual incision reaction in TC-NER.
REACT_340454. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_340969. Formation of the Early Elongation Complex.
REACT_341230. Transcriptional regulation by small RNAs.
REACT_341557. Processing of Capped Intron-Containing Pre-mRNA.
REACT_343091. RNA Polymerase II Transcription Elongation.
REACT_343213. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_345080. Transcription-coupled NER (TC-NER).
REACT_351677. RNA Polymerase II Transcription Initiation.
REACT_353271. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB7
Short name:
RNA polymerase II subunit B7
Alternative name(s):
DNA-directed RNA polymerase II subunit G
Gene namesi
Name:Polr2g
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621284. Polr2g.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172DNA-directed RNA polymerase II subunit RPB7PRO_0000073988Add
BLAST

Proteomic databases

PRIDEiP62489.

Expressioni

Gene expression databases

GenevisibleiP62489. RN.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.

Protein-protein interaction databases

BioGridi250618. 1 interaction.
STRINGi10116.ENSRNOP00000026313.

Structurei

3D structure databases

ProteinModelPortaliP62489.
SMRiP62489. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1095.
GeneTreeiENSGT00390000008975.
HOGENOMiHOG000158201.
HOVERGENiHBG055233.
InParanoidiP62489.
KOiK03015.
OMAiGQAEFVT.
OrthoDBiEOG76MK9S.
PhylomeDBiP62489.
TreeFamiTF103042.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR005576. RNA_pol_Rpb7_N.
IPR022967. S1_dom.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.

Sequencei

Sequence statusi: Complete.

P62489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT
60 70 80 90 100
IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE
110 120 130 140 150
IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT
160 170
RVDKNDIFAI GSLMDDYLGL VS
Length:172
Mass (Da):19,294
Last modified:July 19, 2004 - v1
Checksum:iAF3CE655F658CD5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71925 mRNA. Translation: CAA96468.1.
BC060550 mRNA. Translation: AAH60550.1.
BC060595 mRNA. Translation: AAH60595.1.
RefSeqiNP_446400.1. NM_053948.4.
XP_006231035.1. XM_006230973.1.
UniGeneiRn.106248.

Genome annotation databases

EnsembliENSRNOT00000026313; ENSRNOP00000026313; ENSRNOG00000019439.
GeneIDi117017.
KEGGirno:117017.
UCSCiRGD:621284. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71925 mRNA. Translation: CAA96468.1.
BC060550 mRNA. Translation: AAH60550.1.
BC060595 mRNA. Translation: AAH60595.1.
RefSeqiNP_446400.1. NM_053948.4.
XP_006231035.1. XM_006230973.1.
UniGeneiRn.106248.

3D structure databases

ProteinModelPortaliP62489.
SMRiP62489. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250618. 1 interaction.
STRINGi10116.ENSRNOP00000026313.

Proteomic databases

PRIDEiP62489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026313; ENSRNOP00000026313; ENSRNOG00000019439.
GeneIDi117017.
KEGGirno:117017.
UCSCiRGD:621284. rat.

Organism-specific databases

CTDi5436.
RGDi621284. Polr2g.

Phylogenomic databases

eggNOGiCOG1095.
GeneTreeiENSGT00390000008975.
HOGENOMiHOG000158201.
HOVERGENiHBG055233.
InParanoidiP62489.
KOiK03015.
OMAiGQAEFVT.
OrthoDBiEOG76MK9S.
PhylomeDBiP62489.
TreeFamiTF103042.

Enzyme and pathway databases

ReactomeiREACT_271824. RNA Polymerase II Promoter Escape.
REACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_319326. mRNA Splicing - Minor Pathway.
REACT_319445. RNA Pol II CTD phosphorylation and interaction with CE.
REACT_323062. mRNA Capping.
REACT_323975. Dual incision reaction in TC-NER.
REACT_340454. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
REACT_340969. Formation of the Early Elongation Complex.
REACT_341230. Transcriptional regulation by small RNAs.
REACT_341557. Processing of Capped Intron-Containing Pre-mRNA.
REACT_343091. RNA Polymerase II Transcription Elongation.
REACT_343213. RNA Polymerase II Transcription Initiation And Promoter Clearance.
REACT_345080. Transcription-coupled NER (TC-NER).
REACT_351677. RNA Polymerase II Transcription Initiation.
REACT_353271. mRNA Splicing - Major Pathway.

Miscellaneous databases

NextBioi619759.
PROiP62489.

Gene expression databases

GenevisibleiP62489. RN.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR005576. RNA_pol_Rpb7_N.
IPR022967. S1_dom.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Gillen C., Gleichmann M., Mueller H.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Sciatic nerve.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiRPB7_RAT
AccessioniPrimary (citable) accession number: P62489
Secondary accession number(s): P52433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.