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P62489 (RPB7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase II subunit RPB7

Short name=RNA polymerase II subunit B7
Alternative name(s):
DNA-directed RNA polymerase II subunit G
Gene names
Name:Polr2g
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length172 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA By similarity.

Subunit structure

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   LigandRNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from expression pattern PubMed 17356516. Source: RGD

nuclear-transcribed mRNA catabolic process, exonucleolytic

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of nuclear-transcribed mRNA poly(A) tail shortening

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of translational initiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

transcription initiation from RNA polymerase II promoter

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentDNA-directed RNA polymerase II, core complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic mRNA processing body

Inferred from Biological aspect of Ancestor. Source: RefGenome

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

single-stranded DNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

single-stranded RNA binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

translation initiation factor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 172172DNA-directed RNA polymerase II subunit RPB7
PRO_0000073988

Sequences

Sequence LengthMass (Da)Tools
P62489 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: AF3CE655F658CD5C

FASTA17219,294
        10         20         30         40         50         60 
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT IDNIGAGVIQ 

        70         80         90        100        110        120 
PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE IGPMSCFISR HSIPSEMEFD 

       130        140        150        160        170 
PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT RVDKNDIFAI GSLMDDYLGL VS 

« Hide

References

« Hide 'large scale' references
[1]Gillen C., Gleichmann M., Mueller H.
Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Sciatic nerve.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pituitary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71925 mRNA. Translation: CAA96468.1.
BC060550 mRNA. Translation: AAH60550.1.
BC060595 mRNA. Translation: AAH60595.1.
RefSeqNP_446400.1. NM_053948.4.
XP_006231035.1. XM_006230973.1.
UniGeneRn.106248.

3D structure databases

ProteinModelPortalP62489.
SMRP62489. Positions 1-171.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250618. 1 interaction.

Proteomic databases

PRIDEP62489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026313; ENSRNOP00000026313; ENSRNOG00000019439.
GeneID117017.
KEGGrno:117017.
UCSCRGD:621284. rat.

Organism-specific databases

CTD5436.
RGD621284. Polr2g.

Phylogenomic databases

eggNOGCOG1095.
GeneTreeENSGT00390000008975.
HOGENOMHOG000158201.
HOVERGENHBG055233.
InParanoidP62489.
KOK03015.
OMAKEDHLGV.
OrthoDBEOG76MK9S.
PhylomeDBP62489.
TreeFamTF103042.

Gene expression databases

GenevestigatorP62489.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR022967. RNA-binding_domain_S1.
IPR005576. RNA_pol_Rpb7_N.
[Graphical view]
PfamPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SMARTSM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
ProtoNetSearch...

Other

NextBio619759.
PROP62489.

Entry information

Entry nameRPB7_RAT
AccessionPrimary (citable) accession number: P62489
Secondary accession number(s): P52433
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families