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Protein

DNA-directed RNA polymerase II subunit RPB7

Gene

POLR2G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB7
Short name:
RNA polymerase II subunit B7
Alternative name(s):
DNA-directed RNA polymerase II subunit G
RNA polymerase II 19 kDa subunit
Short name:
RPB19
Gene namesi
Name:POLR2G
Synonyms:RPB7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9194. POLR2G.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasmic mRNA processing body Source: GO_Central
  • DNA-directed RNA polymerase II, core complex Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141H → E: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi33 – 331E → K: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi41 – 411K → E: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi90 – 901T → A: Reduces RNA-binding. 1 Publication
Mutagenesisi93 – 931N → A: Reduces RNA-binding. 1 Publication
Mutagenesisi94 – 941K → E: Reduces RNA-binding. 1 Publication
Mutagenesisi107 – 1071F → E: Reduces RNA-binding. 1 Publication
Mutagenesisi109 – 1091S → A: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi111 – 1111H → E: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi151 – 1511R → E: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi153 – 1531D → E: Strongly reduces RNA-binding. 1 Publication
Mutagenesisi158 – 1581F → A: Strongly reduces RNA-binding. 1 Publication

Organism-specific databases

PharmGKBiPA33514.

Polymorphism and mutation databases

BioMutaiPOLR2G.
DMDMi50403601.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172DNA-directed RNA polymerase II subunit RPB7PRO_0000073986Add
BLAST

Proteomic databases

EPDiP62487.
MaxQBiP62487.
PaxDbiP62487.
PeptideAtlasiP62487.
PRIDEiP62487.

PTM databases

iPTMnetiP62487.
PhosphoSiteiP62487.

Expressioni

Gene expression databases

BgeeiENSG00000168002.
CleanExiHS_POLR2G.
ExpressionAtlasiP62487. baseline and differential.
GenevisibleiP62487. HS.

Organism-specific databases

HPAiHPA053000.

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111432. 92 interactions.
DIPiDIP-32663N.
IntActiP62487. 66 interactions.
MINTiMINT-1033626.
STRINGi9606.ENSP00000301788.

Structurei

Secondary structure

1
172
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1312Combined sources
Helixi15 – 173Combined sources
Helixi22 – 3413Combined sources
Turni40 – 423Combined sources
Beta strandi43 – 5412Combined sources
Beta strandi64 – 7714Combined sources
Beta strandi84 – 9310Combined sources
Beta strandi96 – 1016Combined sources
Beta strandi104 – 1096Combined sources
Helixi110 – 1123Combined sources
Beta strandi117 – 1259Combined sources
Beta strandi127 – 1304Combined sources
Beta strandi135 – 1384Combined sources
Beta strandi142 – 15312Combined sources
Beta strandi156 – 1627Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C35X-ray2.70B/D/F/H1-172[»]
5IY6electron microscopy7.20G1-172[»]
5IY7electron microscopy8.60G1-172[»]
5IY8electron microscopy7.90G1-172[»]
5IY9electron microscopy6.30G1-172[»]
5IYAelectron microscopy5.40G1-172[»]
5IYBelectron microscopy3.90G1-172[»]
5IYCelectron microscopy3.90G1-172[»]
5IYDelectron microscopy3.90G1-172[»]
ProteinModelPortaliP62487.
SMRiP62487. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62487.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3298. Eukaryota.
COG1095. LUCA.
GeneTreeiENSGT00390000008975.
HOGENOMiHOG000158201.
HOVERGENiHBG055233.
InParanoidiP62487.
KOiK03015.
OMAiSYFGPRM.
OrthoDBiEOG091G0MF6.
PhylomeDBiP62487.
TreeFamiTF103042.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR005576. RNA_pol_Rpb7_N.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.

Sequencei

Sequence statusi: Complete.

P62487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT
60 70 80 90 100
IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE
110 120 130 140 150
IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT
160 170
RVDKNDIFAI GSLMDDYLGL VS
Length:172
Mass (Da):19,294
Last modified:July 19, 2004 - v1
Checksum:iAF3CE655F658CD5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20659 mRNA. Translation: AAA86500.1.
U52427 Genomic DNA. Translation: AAB96827.1.
AK312131 mRNA. Translation: BAG35067.1.
CH471076 Genomic DNA. Translation: EAW74092.1.
BC112162 mRNA. Translation: AAI12163.1.
BC112164 mRNA. Translation: AAI12165.1.
CCDSiCCDS31585.1.
RefSeqiNP_002687.1. NM_002696.2.
UniGeneiHs.14839.

Genome annotation databases

EnsembliENST00000301788; ENSP00000301788; ENSG00000168002.
GeneIDi5436.
KEGGihsa:5436.
UCSCiuc001nva.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20659 mRNA. Translation: AAA86500.1.
U52427 Genomic DNA. Translation: AAB96827.1.
AK312131 mRNA. Translation: BAG35067.1.
CH471076 Genomic DNA. Translation: EAW74092.1.
BC112162 mRNA. Translation: AAI12163.1.
BC112164 mRNA. Translation: AAI12165.1.
CCDSiCCDS31585.1.
RefSeqiNP_002687.1. NM_002696.2.
UniGeneiHs.14839.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2C35X-ray2.70B/D/F/H1-172[»]
5IY6electron microscopy7.20G1-172[»]
5IY7electron microscopy8.60G1-172[»]
5IY8electron microscopy7.90G1-172[»]
5IY9electron microscopy6.30G1-172[»]
5IYAelectron microscopy5.40G1-172[»]
5IYBelectron microscopy3.90G1-172[»]
5IYCelectron microscopy3.90G1-172[»]
5IYDelectron microscopy3.90G1-172[»]
ProteinModelPortaliP62487.
SMRiP62487. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111432. 92 interactions.
DIPiDIP-32663N.
IntActiP62487. 66 interactions.
MINTiMINT-1033626.
STRINGi9606.ENSP00000301788.

PTM databases

iPTMnetiP62487.
PhosphoSiteiP62487.

Polymorphism and mutation databases

BioMutaiPOLR2G.
DMDMi50403601.

Proteomic databases

EPDiP62487.
MaxQBiP62487.
PaxDbiP62487.
PeptideAtlasiP62487.
PRIDEiP62487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301788; ENSP00000301788; ENSG00000168002.
GeneIDi5436.
KEGGihsa:5436.
UCSCiuc001nva.4. human.

Organism-specific databases

CTDi5436.
GeneCardsiPOLR2G.
HGNCiHGNC:9194. POLR2G.
HPAiHPA053000.
MIMi602013. gene.
neXtProtiNX_P62487.
PharmGKBiPA33514.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3298. Eukaryota.
COG1095. LUCA.
GeneTreeiENSGT00390000008975.
HOGENOMiHOG000158201.
HOVERGENiHBG055233.
InParanoidiP62487.
KOiK03015.
OMAiSYFGPRM.
OrthoDBiEOG091G0MF6.
PhylomeDBiP62487.
TreeFamiTF103042.

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-113418. Formation of the Early Elongation Complex.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167158. Formation of the HIV-1 Early Elongation Complex.
R-HSA-167160. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167242. Abortive elongation of HIV-1 transcript in the absence of Tat.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-168325. Viral Messenger RNA Synthesis.
R-HSA-203927. MicroRNA (miRNA) biogenesis.
R-HSA-452723. Transcriptional regulation of pluripotent stem cells.
R-HSA-5578749. Transcriptional regulation by small RNAs.
R-HSA-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-6803529. FGFR2 alternative splicing.
R-HSA-6807505. RNA polymerase II transcribes snRNA genes.
R-HSA-72086. mRNA Capping.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-HSA-77075. RNA Pol II CTD phosphorylation and interaction with CE.
R-HSA-8851708. Signaling by FGFR2 IIIa TM.

Miscellaneous databases

ChiTaRSiPOLR2G. human.
EvolutionaryTraceiP62487.
GeneWikiiPOLR2G.
GenomeRNAii5436.
PROiP62487.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168002.
CleanExiHS_POLR2G.
ExpressionAtlasiP62487. baseline and differential.
GenevisibleiP62487. HS.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1490.120. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR005576. RNA_pol_Rpb7_N.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF00575. S1. 1 hit.
PF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF88798. SSF88798. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPB7_HUMAN
AccessioniPrimary (citable) accession number: P62487
Secondary accession number(s): B2R5C0, P52433, Q2M1Z4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.