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Protein

DNA-directed RNA polymerase II subunit RPB7

Gene

POLR2G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB7
Short name:
RNA polymerase II subunit B7
Alternative name(s):
DNA-directed RNA polymerase II subunit G
RNA polymerase II 19 kDa subunit
Short name:
RPB19
Gene namesi
Name:POLR2G
Synonyms:RPB7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000168002.11
HGNCiHGNC:9194 POLR2G
MIMi602013 gene
neXtProtiNX_P62487

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14H → E: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi33E → K: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi41K → E: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi90T → A: Reduces RNA-binding. 1 Publication1
Mutagenesisi93N → A: Reduces RNA-binding. 1 Publication1
Mutagenesisi94K → E: Reduces RNA-binding. 1 Publication1
Mutagenesisi107F → E: Reduces RNA-binding. 1 Publication1
Mutagenesisi109S → A: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi111H → E: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi151R → E: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi153D → E: Strongly reduces RNA-binding. 1 Publication1
Mutagenesisi158F → A: Strongly reduces RNA-binding. 1 Publication1

Organism-specific databases

DisGeNETi5436
OpenTargetsiENSG00000168002
PharmGKBiPA33514

Polymorphism and mutation databases

BioMutaiPOLR2G
DMDMi50403601

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739861 – 172DNA-directed RNA polymerase II subunit RPB7Add BLAST172

Proteomic databases

EPDiP62487
MaxQBiP62487
PaxDbiP62487
PeptideAtlasiP62487
PRIDEiP62487

PTM databases

iPTMnetiP62487
PhosphoSitePlusiP62487

Expressioni

Gene expression databases

BgeeiENSG00000168002
CleanExiHS_POLR2G
ExpressionAtlasiP62487 baseline and differential
GenevisibleiP62487 HS

Organism-specific databases

HPAiHPA053000

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111432, 98 interactors
CORUMiP62487
DIPiDIP-32663N
IntActiP62487, 70 interactors
STRINGi9606.ENSP00000301788

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 13Combined sources12
Helixi15 – 17Combined sources3
Helixi22 – 34Combined sources13
Turni40 – 42Combined sources3
Beta strandi43 – 54Combined sources12
Beta strandi64 – 77Combined sources14
Beta strandi84 – 93Combined sources10
Beta strandi96 – 101Combined sources6
Beta strandi104 – 109Combined sources6
Helixi110 – 112Combined sources3
Beta strandi117 – 125Combined sources9
Beta strandi127 – 130Combined sources4
Beta strandi135 – 138Combined sources4
Beta strandi142 – 153Combined sources12
Beta strandi156 – 162Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C35X-ray2.70B/D/F/H1-172[»]
5IY6electron microscopy7.20G1-172[»]
5IY7electron microscopy8.60G1-172[»]
5IY8electron microscopy7.90G1-172[»]
5IY9electron microscopy6.30G1-172[»]
5IYAelectron microscopy5.40G1-172[»]
5IYBelectron microscopy3.90G1-172[»]
5IYCelectron microscopy3.90G1-172[»]
5IYDelectron microscopy3.90G1-172[»]
ProteinModelPortaliP62487
SMRiP62487
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62487

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3298 Eukaryota
COG1095 LUCA
GeneTreeiENSGT00390000008975
HOGENOMiHOG000158201
HOVERGENiHBG055233
InParanoidiP62487
KOiK03015
OMAiMRQPGLG
OrthoDBiEOG091G0MF6
PhylomeDBiP62487
TreeFamiTF103042

Family and domain databases

Gene3Di3.30.1490.120, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR036898 RNA_pol_Rpb7-like_N_sf
IPR005576 RNA_pol_Rpb7_N
IPR022967 S1_dom
IPR003029 S1_domain
PfamiView protein in Pfam
PF00575 S1, 1 hit
PF03876 SHS2_Rpb7-N, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF88798 SSF88798, 1 hit

Sequencei

Sequence statusi: Complete.

P62487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT
60 70 80 90 100
IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE
110 120 130 140 150
IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT
160 170
RVDKNDIFAI GSLMDDYLGL VS
Length:172
Mass (Da):19,294
Last modified:July 19, 2004 - v1
Checksum:iAF3CE655F658CD5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20659 mRNA Translation: AAA86500.1
U52427 Genomic DNA Translation: AAB96827.1
AK312131 mRNA Translation: BAG35067.1
CH471076 Genomic DNA Translation: EAW74092.1
BC112162 mRNA Translation: AAI12163.1
BC112164 mRNA Translation: AAI12165.1
CCDSiCCDS31585.1
RefSeqiNP_002687.1, NM_002696.2
UniGeneiHs.14839

Genome annotation databases

EnsembliENST00000301788; ENSP00000301788; ENSG00000168002
GeneIDi5436
KEGGihsa:5436
UCSCiuc001nva.4 human

Similar proteinsi

Entry informationi

Entry nameiRPB7_HUMAN
AccessioniPrimary (citable) accession number: P62487
Secondary accession number(s): B2R5C0, P52433, Q2M1Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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