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P62487

- RPB7_HUMAN

UniProt

P62487 - RPB7_HUMAN

Protein

DNA-directed RNA polymerase II subunit RPB7

Gene

POLR2G

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA By similarity. Binds RNA.By similarity1 Publication

    GO - Molecular functioni

    1. DNA-directed RNA polymerase activity Source: UniProtKB-KW
    2. single-stranded DNA binding Source: RefGenome
    3. single-stranded RNA binding Source: RefGenome
    4. translation initiation factor binding Source: RefGenome

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. apoptotic process Source: Ensembl
    3. DNA repair Source: Reactome
    4. gene expression Source: Reactome
    5. mRNA splicing, via spliceosome Source: Reactome
    6. nuclear-transcribed mRNA catabolic process, exonucleolytic Source: RefGenome
    7. nucleotide-excision repair Source: Reactome
    8. positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: RefGenome
    9. positive regulation of translational initiation Source: RefGenome
    10. positive regulation of viral transcription Source: Reactome
    11. RNA splicing Source: Reactome
    12. transcription-coupled nucleotide-excision repair Source: Reactome
    13. transcription elongation from RNA polymerase II promoter Source: Reactome
    14. transcription from RNA polymerase II promoter Source: UniProtKB
    15. transcription initiation from RNA polymerase II promoter Source: RefGenome
    16. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB7
    Short name:
    RNA polymerase II subunit B7
    Alternative name(s):
    DNA-directed RNA polymerase II subunit G
    RNA polymerase II 19 kDa subunit
    Short name:
    RPB19
    Gene namesi
    Name:POLR2G
    Synonyms:RPB7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:9194. POLR2G.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasmic mRNA processing body Source: RefGenome
    2. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    3. nucleolus Source: HPA
    4. nucleoplasm Source: Reactome
    5. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi14 – 141H → E: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi33 – 331E → K: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi41 – 411K → E: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi90 – 901T → A: Reduces RNA-binding. 1 Publication
    Mutagenesisi93 – 931N → A: Reduces RNA-binding. 1 Publication
    Mutagenesisi94 – 941K → E: Reduces RNA-binding. 1 Publication
    Mutagenesisi107 – 1071F → E: Reduces RNA-binding. 1 Publication
    Mutagenesisi109 – 1091S → A: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi111 – 1111H → E: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi151 – 1511R → E: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi153 – 1531D → E: Strongly reduces RNA-binding. 1 Publication
    Mutagenesisi158 – 1581F → A: Strongly reduces RNA-binding. 1 Publication

    Organism-specific databases

    PharmGKBiPA33514.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 172172DNA-directed RNA polymerase II subunit RPB7PRO_0000073986Add
    BLAST

    Proteomic databases

    MaxQBiP62487.
    PaxDbiP62487.
    PeptideAtlasiP62487.
    PRIDEiP62487.

    PTM databases

    PhosphoSiteiP62487.

    Expressioni

    Gene expression databases

    ArrayExpressiP62487.
    BgeeiP62487.
    CleanExiHS_POLR2G.
    GenevestigatoriP62487.

    Organism-specific databases

    HPAiHPA053000.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex.1 Publication

    Protein-protein interaction databases

    BioGridi111432. 46 interactions.
    DIPiDIP-32663N.
    IntActiP62487. 20 interactions.
    MINTiMINT-1033626.
    STRINGi9606.ENSP00000301788.

    Structurei

    Secondary structure

    1
    172
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312
    Helixi15 – 173
    Helixi22 – 3413
    Turni40 – 423
    Beta strandi43 – 5412
    Beta strandi64 – 7714
    Beta strandi84 – 9310
    Beta strandi96 – 1016
    Beta strandi104 – 1096
    Helixi110 – 1123
    Beta strandi117 – 1259
    Beta strandi127 – 1304
    Beta strandi135 – 1384
    Beta strandi142 – 15312
    Beta strandi156 – 1627

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2C35X-ray2.70B/D/F/H1-172[»]
    ProteinModelPortaliP62487.
    SMRiP62487. Positions 1-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62487.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1095.
    HOGENOMiHOG000158201.
    HOVERGENiHBG055233.
    InParanoidiP62487.
    KOiK03015.
    OMAiNNVNKMG.
    OrthoDBiEOG76MK9S.
    PhylomeDBiP62487.
    TreeFamiTF103042.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1490.120. 1 hit.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR022967. RNA-binding_domain_S1.
    IPR005576. RNA_pol_Rpb7_N.
    [Graphical view]
    PfamiPF00575. S1. 1 hit.
    PF03876. SHS2_Rpb7-N. 1 hit.
    [Graphical view]
    SMARTiSM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF88798. SSF88798. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P62487-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFYHISLEHE ILLHPRYFGP NLLNTVKQKL FTEVEGTCTG KYGFVIAVTT    50
    IDNIGAGVIQ PGRGFVLYPV KYKAIVFRPF KGEVVDAVVT QVNKVGLFTE 100
    IGPMSCFISR HSIPSEMEFD PNSNPPCYKT MDEDIVIQQD DEIRLKIVGT 150
    RVDKNDIFAI GSLMDDYLGL VS 172
    Length:172
    Mass (Da):19,294
    Last modified:July 19, 2004 - v1
    Checksum:iAF3CE655F658CD5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20659 mRNA. Translation: AAA86500.1.
    U52427 Genomic DNA. Translation: AAB96827.1.
    AK312131 mRNA. Translation: BAG35067.1.
    CH471076 Genomic DNA. Translation: EAW74092.1.
    BC112162 mRNA. Translation: AAI12163.1.
    BC112164 mRNA. Translation: AAI12165.1.
    CCDSiCCDS31585.1.
    RefSeqiNP_002687.1. NM_002696.2.
    UniGeneiHs.14839.

    Genome annotation databases

    EnsembliENST00000301788; ENSP00000301788; ENSG00000168002.
    GeneIDi5436.
    KEGGihsa:5436.
    UCSCiuc001nva.3. human.

    Polymorphism databases

    DMDMi50403601.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20659 mRNA. Translation: AAA86500.1 .
    U52427 Genomic DNA. Translation: AAB96827.1 .
    AK312131 mRNA. Translation: BAG35067.1 .
    CH471076 Genomic DNA. Translation: EAW74092.1 .
    BC112162 mRNA. Translation: AAI12163.1 .
    BC112164 mRNA. Translation: AAI12165.1 .
    CCDSi CCDS31585.1.
    RefSeqi NP_002687.1. NM_002696.2.
    UniGenei Hs.14839.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2C35 X-ray 2.70 B/D/F/H 1-172 [» ]
    ProteinModelPortali P62487.
    SMRi P62487. Positions 1-171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111432. 46 interactions.
    DIPi DIP-32663N.
    IntActi P62487. 20 interactions.
    MINTi MINT-1033626.
    STRINGi 9606.ENSP00000301788.

    PTM databases

    PhosphoSitei P62487.

    Polymorphism databases

    DMDMi 50403601.

    Proteomic databases

    MaxQBi P62487.
    PaxDbi P62487.
    PeptideAtlasi P62487.
    PRIDEi P62487.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000301788 ; ENSP00000301788 ; ENSG00000168002 .
    GeneIDi 5436.
    KEGGi hsa:5436.
    UCSCi uc001nva.3. human.

    Organism-specific databases

    CTDi 5436.
    GeneCardsi GC11P062529.
    HGNCi HGNC:9194. POLR2G.
    HPAi HPA053000.
    MIMi 602013. gene.
    neXtProti NX_P62487.
    PharmGKBi PA33514.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1095.
    HOGENOMi HOG000158201.
    HOVERGENi HBG055233.
    InParanoidi P62487.
    KOi K03015.
    OMAi NNVNKMG.
    OrthoDBi EOG76MK9S.
    PhylomeDBi P62487.
    TreeFami TF103042.

    Enzyme and pathway databases

    Reactomei REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    EvolutionaryTracei P62487.
    GeneWikii POLR2G.
    GenomeRNAii 5436.
    NextBioi 21033.
    PROi P62487.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62487.
    Bgeei P62487.
    CleanExi HS_POLR2G.
    Genevestigatori P62487.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1490.120. 1 hit.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR022967. RNA-binding_domain_S1.
    IPR005576. RNA_pol_Rpb7_N.
    [Graphical view ]
    Pfami PF00575. S1. 1 hit.
    PF03876. SHS2_Rpb7-N. 1 hit.
    [Graphical view ]
    SMARTi SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF88798. SSF88798. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology."
      Khazak V., Sadhale P.P., Woychik N.A., Brent R., Golemis E.A.
      Mol. Biol. Cell 6:759-775(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Human gene for the RNA polymerase II seventh subunit (hsRPB7): structure, expression and chromosomal localization."
      Schoen T.J., Chandrasekharappa S.C., Guru S.C., Mazuruk K., Chader G.J., Rodriguez I.R.
      Biochim. Biophys. Acta 1353:39-49(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cerebellum.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
      Kershnar E., Wu S.-Y., Chiang C.-M.
      J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II."
      Meka H., Werner F., Cordell S.C., Onesti S., Brick P.
      Nucleic Acids Res. 33:6435-6444(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF THE POL II RPB4-RPB7 SUBCOMPLEX, RNA-BINDING, MUTAGENESIS OF HIS-14; GLU-33; LYS-41; THR-90; ASN-93; LYS-94; PHE-107; SER-109; HIS-111; ARG-151; ASP-153 AND PHE-158.

    Entry informationi

    Entry nameiRPB7_HUMAN
    AccessioniPrimary (citable) accession number: P62487
    Secondary accession number(s): B2R5C0, P52433, Q2M1Z4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3