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Protein

Abl interactor 2

Gene

Abi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.1 Publication

GO - Molecular functioni

  1. proline-rich region binding Source: MGI
  2. protein complex binding Source: MGI
  3. SH3 domain binding Source: MGI
  4. ubiquitin protein ligase binding Source: MGI

GO - Biological processi

  1. actin polymerization or depolymerization Source: InterPro
  2. camera-type eye development Source: MGI
  3. cell migration Source: MGI
  4. dendrite development Source: MGI
  5. learning or memory Source: MGI
  6. peptidyl-tyrosine phosphorylation Source: MGI
  7. positive regulation of Arp2/3 complex-mediated actin nucleation Source: MGI
  8. Rac protein signal transduction Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Abl interactor 2
Alternative name(s):
Abelson interactor 2
Short name:
Abi-2
Gene namesi
Name:Abi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:106913. Abi2.

Subcellular locationi

Cytoplasm. Cell projectionlamellipodium. Cell projectionfilopodium. Cytoplasmcytoskeleton
Note: Localized to protruding lamellipodia and filopodia tips.

GO - Cellular componenti

  1. cell-cell adherens junction Source: MGI
  2. cytoskeleton Source: UniProtKB-SubCell
  3. cytosol Source: MGI
  4. dendrite Source: MGI
  5. filopodium Source: MGI
  6. lamellipodium Source: MGI
  7. SCAR complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Abl interactor 2PRO_0000191791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211PhosphoserineBy similarity
Modified residuei301 – 3011PhosphoserineBy similarity

Post-translational modificationi

Is a substrate for ABL1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP62484.
PaxDbiP62484.
PRIDEiP62484.

PTM databases

PhosphoSiteiP62484.

Expressioni

Tissue specificityi

Expresses in embryonic and adult brain. In adult brain prominently expressed in the neocortex, hippocampus and dentate gyrus.1 Publication

Developmental stagei

Detected at E10 in developing brain, and expression is more prominent in the neuroepithelium compared to the surrounding tissue. At E12 expression is enhanced throughout the CNS and is detected along the full length of the spinal chord. At E16 expression remains enhanced in the CNS, and is particularly prominent in the olfactory bulb. Also highly expressed in dorsal root ganglia.1 Publication

Gene expression databases

BgeeiP62484.
CleanExiMM_ABI2.
ExpressionAtlasiP62484. baseline and differential.
GenevestigatoriP62484.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Interacts with ABL1 and ABL2.By similarity

Protein-protein interaction databases

DIPiDIP-48418N.

Structurei

3D structure databases

ProteinModelPortaliP62484.
SMRiP62484. Positions 1-155, 377-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10763t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST
Domaini384 – 44360SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi166 – 356191Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the ABI family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG262939.
GeneTreeiENSGT00390000003756.
HOGENOMiHOG000293213.
HOVERGENiHBG050446.
InParanoidiP62484.
KOiK05751.
OMAiPWAPRAY.
OrthoDBiEOG7J17ZT.
PhylomeDBiP62484.
TreeFamiTF314303.

Family and domain databases

InterProiIPR028457. ABI.
IPR028454. Abi2.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR10460. PTHR10460. 1 hit.
PTHR10460:SF3. PTHR10460:SF3. 1 hit.
PfamiPF07815. Abi_HHR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELQMLLEE EIPGGRRALF DSYTNLERVA DYCENNYIQS PDKQRALEET
60 70 80 90 100
KAYTTQSLAS VAYLINTLAN NVLQMLDIQA SQLRRMESSI NHISQTVDIH
110 120 130 140 150
KEKVARREIG ILTTNKNTSR THKIIAPANL ERPVRYIRKP IDYTILDDIG
160 170 180 190 200
HGVKVSTQNM KMGGLPRTTP PTQKPPSPPM SGKGTLGRHS PYRTLEPVRP
210 220 230 240 250
PVVPNDYVPS PTRNMAPSQQ SPVRTASVNQ RNRTYSSSGS SGGSHPSSRS
260 270 280 290 300
SSRENSGSGS VGVPIAVPTP SPPSVFPGHP VQFYSMNRPA SRHTPPTIGG
310 320 330 340 350
SLPYRRPPSI TSQTSLQNQM NGGPFYNQNP VSDTPPPPPP VEEPVFDESP
360 370 380 390 400
PPPPPPEDYE EEEAAVVEYS DPYAEEDPPW APRAYLEKVV AIYDYTKDKE
410 420 430 440
DELSFQEGAI IYVIKKNDDG WYEGVMNGVT GLFPGNYVES IMHYSE
Length:446
Mass (Da):49,387
Last modified:July 18, 2004 - v1
Checksum:i8EC1FB543D93BEAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056345 mRNA. Translation: AAH56345.1.
CCDSiCCDS14991.1.
RefSeqiNP_001185499.1. NM_001198570.1.
NP_001185500.1. NM_001198571.1.
NP_937760.1. NM_198127.2.
UniGeneiMm.212066.
Mm.465571.

Genome annotation databases

EnsembliENSMUST00000052332; ENSMUSP00000058754; ENSMUSG00000026782.
GeneIDi329165.
KEGGimmu:329165.
UCSCiuc007beq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC056345 mRNA. Translation: AAH56345.1.
CCDSiCCDS14991.1.
RefSeqiNP_001185499.1. NM_001198570.1.
NP_001185500.1. NM_001198571.1.
NP_937760.1. NM_198127.2.
UniGeneiMm.212066.
Mm.465571.

3D structure databases

ProteinModelPortaliP62484.
SMRiP62484. Positions 1-155, 377-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48418N.

PTM databases

PhosphoSiteiP62484.

Proteomic databases

MaxQBiP62484.
PaxDbiP62484.
PRIDEiP62484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052332; ENSMUSP00000058754; ENSMUSG00000026782.
GeneIDi329165.
KEGGimmu:329165.
UCSCiuc007beq.2. mouse.

Organism-specific databases

CTDi10152.
MGIiMGI:106913. Abi2.

Phylogenomic databases

eggNOGiNOG262939.
GeneTreeiENSGT00390000003756.
HOGENOMiHOG000293213.
HOVERGENiHBG050446.
InParanoidiP62484.
KOiK05751.
OMAiPWAPRAY.
OrthoDBiEOG7J17ZT.
PhylomeDBiP62484.
TreeFamiTF314303.

Miscellaneous databases

NextBioi398613.
PROiP62484.
SOURCEiSearch...

Gene expression databases

BgeeiP62484.
CleanExiMM_ABI2.
ExpressionAtlasiP62484. baseline and differential.
GenevestigatoriP62484.

Family and domain databases

InterProiIPR028457. ABI.
IPR028454. Abi2.
IPR012849. Abl-interactor_HHR_dom.
IPR001452. SH3_domain.
IPR000727. T_SNARE_dom.
[Graphical view]
PANTHERiPTHR10460. PTHR10460. 1 hit.
PTHR10460:SF3. PTHR10460:SF3. 1 hit.
PfamiPF07815. Abi_HHR. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia."
    Stradal T.E.B., Courtney K.D., Rottner K., Hahne P., Small J.V., Pendergast A.M.
    Curr. Biol. 11:891-895(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Abl interactor 1 (Abi-1) wave-binding and SNARE domains regulate its nucleocytoplasmic shuttling, lamellipodium localization, and wave-1 levels."
    Echarri A., Lai M.J., Robinson M.R., Pendergast A.M.
    Mol. Cell. Biol. 24:4979-4993(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system."
    Courtney K.D., Grove M., Vandongen H., Vandongen A., LaMantia A.-S., Pendergast A.M.
    Mol. Cell. Neurosci. 16:244-257(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiABI2_MOUSE
AccessioniPrimary (citable) accession number: P62484
Secondary accession number(s): Q6PHU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2004
Last sequence update: July 18, 2004
Last modified: March 3, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.