Abl interactor 2 - Mus musculus (Mouse)

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P62484 (ABI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 83. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Abl interactor 2

Alternative name(s):
Abelson interactor 2
Short name=Abi-2

Gene names

Name:

Abi2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA. Ref.3
Subunit structure	Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 By similarity. Interacts with ABL1 and ABL2.
Subcellular location	Cytoplasm. Cell projection › lamellipodium. Cell projection › filopodium. Cytoplasm › cytoskeleton. Note: Localized to protruding lamellipodia and filopodia tips. Ref.2 Ref.3
Tissue specificity	Expresses in embryonic and adult brain. In adult brain prominently expressed in the neocortex, hippocampus and dentate gyrus. Ref.4
Developmental stage	Detected at E10 in developing brain, and expression is more prominent in the neuroepithelium compared to the surrounding tissue. At E12 expression is enhanced throughout the CNS and is detected along the full length of the spinal chord. At E16 expression remains enhanced in the CNS, and is particularly prominent in the olfactory bulb. Also highly expressed in dorsal root ganglia. Ref.4
Post-translational modification	Is a substrate for ABL1 By similarity.
Sequence similarities	Belongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	camera-type eye development Inferred from mutant phenotype PubMed 15572692. Source: MGI cell migration Inferred from mutant phenotype PubMed 15572692. Source: MGI dendrite development Inferred from mutant phenotype PubMed 15572692. Source: MGI learning or memory Inferred from mutant phenotype PubMed 15572692. Source: MGI peptidyl-tyrosine phosphorylation Inferred from sequence alignment PubMed 17101133. Source: MGI
Cellular_component	cell-cell adherens junction Inferred from direct assay PubMed 15572692. Source: MGI cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from sequence alignment PubMed 17101133. Source: MGI dendrite Inferred from direct assay PubMed 15572692. Source: MGI filopodium Inferred from electronic annotation. Source: UniProtKB-SubCell lamellipodium Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 446

446

Abl interactor 2

PRO_0000191791

Regions

Domain

45 – 107

t-SNARE coiled-coil homology

Domain

384 – 443

SH3

Compositional bias

166 – 356

191

Pro-rich

Amino acid modifications

Modified residue

221

Phosphoserine By similarity

Modified residue

301

Phosphoserine By similarity

Modified residue

395

Phosphotyrosine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P62484 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 8EC1FB543D93BEAA
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FASTA

446

49,387

        10         20         30         40         50         60 
MAELQMLLEE EIPGGRRALF DSYTNLERVA DYCENNYIQS PDKQRALEET KAYTTQSLAS 

        70         80         90        100        110        120 
VAYLINTLAN NVLQMLDIQA SQLRRMESSI NHISQTVDIH KEKVARREIG ILTTNKNTSR 

       130        140        150        160        170        180 
THKIIAPANL ERPVRYIRKP IDYTILDDIG HGVKVSTQNM KMGGLPRTTP PTQKPPSPPM 

       190        200        210        220        230        240 
SGKGTLGRHS PYRTLEPVRP PVVPNDYVPS PTRNMAPSQQ SPVRTASVNQ RNRTYSSSGS 

       250        260        270        280        290        300 
SGGSHPSSRS SSRENSGSGS VGVPIAVPTP SPPSVFPGHP VQFYSMNRPA SRHTPPTIGG 

       310        320        330        340        350        360 
SLPYRRPPSI TSQTSLQNQM NGGPFYNQNP VSDTPPPPPP VEEPVFDESP PPPPPPEDYE 

       370        380        390        400        410        420 
EEEAAVVEYS DPYAEEDPPW APRAYLEKVV AIYDYTKDKE DELSFQEGAI IYVIKKNDDG 

       430        440 
WYEGVMNGVT GLFPGNYVES IMHYSE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia." Stradal T.E.B., Courtney K.D., Rottner K., Hahne P., Small J.V., Pendergast A.M. Curr. Biol. 11:891-895(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Abl interactor 1 (Abi-1) wave-binding and SNARE domains regulate its nucleocytoplasmic shuttling, lamellipodium localization, and wave-1 levels." Echarri A., Lai M.J., Robinson M.R., Pendergast A.M. Mol. Cell. Biol. 24:4979-4993(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"Localization and phosphorylation of Abl-interactor proteins, Abi-1 and Abi-2, in the developing nervous system." Courtney K.D., Grove M., Vandongen H., Vandongen A., LaMantia A.-S., Pendergast A.M. Mol. Cell. Neurosci. 16:244-257(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-395, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC056345 mRNA. Translation: AAH56345.1.
IPI	IPI00380956.
RefSeq	NP_001185499.1. NM_001198570.1. NP_001185500.1. NM_001198571.1. NP_937760.1. NM_198127.2.
UniGene	Mm.212066. Mm.465571.
3D structure databases
ProteinModelPortal	P62484.
SMR	P62484. Positions 1-155, 377-441.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48418N.
PTM databases
PhosphoSite	P62484.
Proteomic databases
PaxDb	P62484.
PRIDE	P62484.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000052332; ENSMUSP00000058754; ENSMUSG00000026782.
GeneID	329165.
KEGG	mmu:329165.
UCSC	uc007beq.2. mouse.
Organism-specific databases
CTD	10152.
MGI	MGI:106913. Abi2.
Phylogenomic databases
eggNOG	NOG262939.
GeneTree	ENSGT00390000003756.
HOGENOM	HOG000293213.
HOVERGEN	HBG050446.
KO	K05751.
OMA	AGHPVQF.
OrthoDB	EOG4ZKJMQ.
Gene expression databases
Bgee	P62484.
CleanEx	MM_ABI2.
Genevestigator	P62484.
GermOnline	ENSMUSG00000026782. Mus musculus.
Family and domain databases
InterPro	IPR012849. Abl-interactor_HHR_dom. IPR001452. SH3_domain. IPR000727. T_SNARE_dom. [Graphical view]
Pfam	PF07815. Abi_HHR. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	398613.
SOURCE	Search...

Entry information

Entry name

ABI2_MOUSE

Accession

Primary (citable) accession number: P62484
Secondary accession number(s): Q6PHU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents