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Protein

Voltage-gated potassium channel subunit beta-2

Gene

Kcnab2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:8576199). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (PubMed:11825900, PubMed:21209188). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:8576199). Modulates the functional properties of KCNA4 (By similarity). Modulates the functional properties of KCNA5 (PubMed:8576199). Enhances KCNB2 channel activity (PubMed:8824288). Modulates the functional properties of KCNA5 (PubMed:8576199). Binds NADPH and has NADPH-dependent aldoketoreductase activity (By similarity). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro) (By similarity).By similarity4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63NADPBy similarity1
Binding sitei85NADPBy similarity1
Active sitei90Proton donor/acceptorBy similarity1
Binding sitei90NADPBy similarity1
Binding sitei214NADPBy similarity1
Binding sitei254NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 57NADPBy similarity2
Nucleotide bindingi188 – 189NADPBy similarity2
Nucleotide bindingi243 – 248NADPBy similarity6
Nucleotide bindingi323 – 329NADPBy similarity7

GO - Molecular functioni

GO - Biological processi

  • hematopoietic progenitor cell differentiation Source: MGI
  • NADPH oxidation Source: UniProtKB
  • neuromuscular process Source: UniProtKB
  • oxidation-reduction process Source: UniProtKB
  • regulation of potassium ion transmembrane transport Source: UniProtKB
  • regulation of protein localization to cell surface Source: UniProtKB

Keywordsi

Molecular functionIon channel, Oxidoreductase, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandNADP, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296072 Voltage gated Potassium channels
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel subunit beta-2 (EC:1.1.1.-By similarity)
Alternative name(s):
K(+) channel subunit beta-2
Kv-beta-2
Neuroimmune protein F5
Gene namesi
Name:Kcnab2
Synonyms:Ckbeta2, I2rf5, Kcnb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:109239 Kcnab2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mutant mice are born at the expected Mendelian rate and are fertile, but exhibit occasional seizures and have a median lifespan of 255 days, which is much shorter than the 400 days typically observed for heterozygotes (PubMed:11825900). The reduction in lifespan depends strongly on the genetic background; median survival is 138 days for B6 mice, 255 days for B6/129 mice and over 400 days for 129/SvEv mice (PubMed:15720404). Mice exhibit whole body tremors after swimming in cold water, which is not observed in wild-type (PubMed:11825900). The whole body tremors observed after swimming in cold water differ between mouse strains; the observed differences are largely due to differences in the decrease of the core body temperature (PubMed:15720404). Mice lacking both KCNAB1 and KCNAB2 have a median survival of 114 days instead of the 255 days observed for mice lacking only KCNAB2, but show no aggravation of the whole body tremors observed after swimming in cold water (PubMed:15720404). Mice lacking KCNAB2 show subtle deficits in associative learning and aberrant excitability of neurons from the lateral amygdala (PubMed:21209188).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90Y → F: No detectable phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001487471 – 367Voltage-gated potassium channel subunit beta-2Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei14PhosphoserineBy similarity1
Modified residuei20Phosphoserine1 Publication1
Modified residuei28Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei28Omega-N-methylarginine; alternateCombined sources1
Modified residuei31PhosphoserineBy similarity1
Modified residuei112Phosphoserine1 Publication1
Modified residuei124N6-acetyllysineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCZ; may be regulated by incorporation in a complex composed of PRKCZ and SQSTM1.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiP62482
MaxQBiP62482
PaxDbiP62482
PeptideAtlasiP62482
PRIDEiP62482

PTM databases

iPTMnetiP62482
PhosphoSitePlusiP62482
SwissPalmiP62482

Expressioni

Tissue specificityi

Detected in brain (PubMed:21357749). Detected at basket cell terminals in cerebellum and in the juxtaparanodal region of nodes of Ranvier (at protein level) (PubMed:11825900). Strongest expression in brain and eye. Highest levels in brain detected in brainstem and diencephalon. Strong expression also detected in lung and heart. Moderate expression in kidney, T-lymphocytes and skeletal muscle.4 Publications

Developmental stagei

Not detected prior to birth, low levels of expression detected from postnatal days 1 to 7. Expression reaches adult levels by postnatal day 21.1 Publication

Gene expression databases

BgeeiENSMUSG00000028931
ExpressionAtlasiP62482 baseline and differential
GenevisibleiP62482 MM

Interactioni

Subunit structurei

Homotetramer (By similarity). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (By similarity). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (By similarity). Interacts with KCNA1 (By similarity). Interacts with KCNA2 (By similarity). Interacts with KCNA4 and KCND3 (By similarity). Interacts (in unphosphorylated form) with MAPRE1 (By similarity). Interacts with KCNA5 (PubMed:8576199, PubMed:8824288). Interacts with KCNB2 (PubMed:8824288). Forms a ternary complex with SQSTM1 and PRKCZ (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi200884, 5 interactors
IntActiP62482, 7 interactors
MINTiP62482
STRINGi10090.ENSMUSP00000101273

Structurei

3D structure databases

ProteinModelPortaliP62482
SMRiP62482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

In contrast to KCNAB1, the shorter N-terminal domain of KCNAB2 cannot mediate closure of delayed rectifier potassium channels by physically obstructing the pore.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1575 Eukaryota
COG0667 LUCA
GeneTreeiENSGT00550000074567
HOGENOMiHOG000250283
HOVERGENiHBG052216
InParanoidiP62482
KOiK04883
PhylomeDBiP62482
TreeFamiTF324563

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR005983 K_chnl_volt-dep_bsu_KCNAB
IPR005399 K_chnl_volt-dep_bsu_KCNAB-rel
IPR005401 K_chnl_volt-dep_bsu_KCNAB2
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PRINTSiPR01579 KCNAB2CHANEL
PR01577 KCNABCHANNEL
SUPFAMiSSF51430 SSF51430, 1 hit
TIGRFAMsiTIGR01293 Kv_beta, 1 hit

Sequencei

Sequence statusi: Complete.

P62482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS
60 70 80 90 100
CLGLGTWVTF GGQITDEMAE HLMTLAYDNG INLFDTAEVY AAGKAEVVLG
110 120 130 140 150
NIIKKKGWRR SSLVITTKIF WGGKAETERG LSRKHIIEGL KASLERLQLE
160 170 180 190 200
YVDVVFANRP DPNTPMEETV RAMTHVINQG MAMYWGTSRW SSMEIMEAYS
210 220 230 240 250
VARQFNLIPP ICEQAEYHMF QREKVEVQLP ELFHKIGVGA MTWSPLACGI
260 270 280 290 300
VSGKYDSGIP PYSRASLKGY QWLKDKILSE EGRRQQAKLK ELQAIAERLG
310 320 330 340 350
CTLPQLAIAW CLRNEGVSSV LLGASNAEQL MENIGAIQVL PKLSSSIVHE
360
IDSILGNKPY SKKDYRS
Length:367
Mass (Da):41,021
Last modified:July 5, 2004 - v1
Checksum:i5303FD1411B324FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64I → L in AAB37263 (PubMed:8824288).Curated1
Sequence conflicti99 – 100LG → FR in AAA75174 (PubMed:1573677).Curated2
Sequence conflicti202A → G in AAB37263 (PubMed:8824288).Curated1
Sequence conflicti266S → L in AAA75174 (PubMed:1573677).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L48983 mRNA Translation: AAB00829.1
U65592 mRNA Translation: AAB37263.1
U31908 mRNA Translation: AAA75174.1
BC039178 mRNA Translation: AAH39178.1
CCDSiCCDS19000.1
RefSeqiNP_001239585.1, NM_001252656.1
NP_034728.2, NM_010598.3
XP_017175485.1, XM_017319996.1
UniGeneiMm.388924

Genome annotation databases

EnsembliENSMUST00000105648; ENSMUSP00000101273; ENSMUSG00000028931
ENSMUST00000160884; ENSMUSP00000125058; ENSMUSG00000028931
GeneIDi16498
KEGGimmu:16498
UCSCiuc008wal.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKCAB2_MOUSE
AccessioniPrimary (citable) accession number: P62482
Secondary accession number(s): P97381, Q60942, Q64284
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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