P62403 (RL5_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L5

Gene names

Name:	rplE
Ordered Locus Names:	Rv0716, MT0742
ORF Names:	MTCY210.35

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs By similarity. HAMAP MF_01333_B
Subunit structure	Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L5P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE translation Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	50S ribosomal protein L5 HAMAP MF_01333_B		PRO_0000124956

Sequences

	Sequence		Length	Mass (Da)
	P62403 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: CFC6FDB2718308BC	FASTA	187	21,019
	10 20 30 40 50 60 MTTAQKVQPR LKERYRSEIR DALRKQFGYG NVMQIPTVTK VVVNMGVGEA ARDAKLINGA 70 80 90 100 110 120 VNDLALITGQ KPEVRRARKS IAQFKLREGM PVGVRVTLRG DRMWEFLDRL TSIALPRIRD 130 140 150 160 170 180 FRGLSPKQFD GVGNYTFGLA EQAVFHEVDV DKIDRVRGMD INVVTSAATD DEGRALLRAL GFPFKEN

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842574 Genomic DNA. Translation: CAB06440.1. AE000516 Genomic DNA. Translation: AAK44975.1.
PIR	G70643.
RefSeq	NP_215230.1. NC_000962.2. NP_335161.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P62403.
SMR	P62403. Positions 8-186.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002122; EBMYCP00000002122; EBMYCG00000002120. EBMYCT00000069791; EBMYCP00000067850; EBMYCG00000069786.
GeneID	888400. 926034.
GenomeReviews	Gene locus MT0742 in contig AE000516_GR. Gene locus Rv0716 in contig AL123456_GR.
KEGG	mtc:MT0742. mtu:Rv0716.
PATRIC	18123365. VBIMycTub22151_0812.
TIGR	MT0742.
Organism-specific databases
TubercuList	Rv0716.
Phylogenomic databases
GeneTree	EBGT00050000016450.
HOGENOM	HBG592167.
OMA	RQGMPIG.
PhylomeDB	P62403.
ProtClustDB	PRK00010.
Family and domain databases
HAMAP	MF_01333_B. Ribosomal_L5_B. [Tree]
InterPro	IPR002132. Ribosomal_L5. IPR020930. Ribosomal_L5_bac-type. IPR022803. Ribosomal_L5_domain. [Graphical view]
Gene3D	G3DSA:3.30.1440.10. Ribosomal_L5. 1 hit.
KO	K02931.
PANTHER	PTHR11994. Ribosomal_L5. 1 hit.
Pfam	PF00281. Ribosomal_L5. 1 hit. PF00673. Ribosomal_L5_C. 1 hit. [Graphical view]
PIRSF	PIRSF002161. Ribosomal_L5. 1 hit.
SUPFAM	SSF55282. Ribosomal_L5. 1 hit.
PROSITE	PS00358. RIBOSOMAL_L5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL5_MYCTU

Accession

Primary (citable) accession number: P62403
Secondary accession number(s): P95064

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program