Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Secretion monitor

Gene

secM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates secA expression by translational coupling of the secM secA operon. Ribosomes translating the C-terminal region of secM can disrupt an RNA repressor helix that normally blocks secA translation initiation, derepressing the expression of secA. Translational pausing of secM at Pro-166 under secretion-limiting conditions increases the duration of the disruption and thus increases secA expression. This is controlled by interaction of the secM signal peptide with secA and the translocon, possibly by secA pulling the paused secM out of the ribosome. The arrest sequence (150-FXXXXWIXXXXGIRAGP-166) is sufficient to cause arrest of unrelated proteins. Elongation arrest can be alleviated by mutations in the 23S rRNA or in ribosomal protein L22.

GO - Molecular functioni

  • translation regulator activity Source: EcoCyc

GO - Biological processi

  • regulation of translation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11087-MONOMER.
ECOL316407:JW5007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Secretion monitor
Gene namesi
Name:secM
Synonyms:srrA, yacA
Ordered Locus Names:b0097, JW5007
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11087. secM.

Subcellular locationi

  • Cytoplasmcytosol 1 Publication
  • Periplasm 1 Publication

  • Note: The active form is cytosolic, while the periplasmic form is rapidly degraded, mainly by the tail-specific protease.

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → V: Protein is not produced, (and therefore neither is SecA) indicating this is the start codon. 1 Publication
Mutagenesisi24 – 241M → C: Protein is produced; this is not the start codon. 1 Publication
Mutagenesisi29 – 335Missing : Loss of the ability to repress SecA-LacZ fusion proteins, translational pausing is very long, with almost no secretion. 1 Publication
Mutagenesisi31 – 344Missing : Loss of the ability to repress SecA-LacZ fusion proteins. 1 Publication
Mutagenesisi163 – 1631R → A: Abolishes translation arrest. 1 Publication
Mutagenesisi166 – 1705Missing : Abolishes translation arrest. 1 Publication
Mutagenesisi166 – 1661P → A: Abolishes translation arrest. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence analysisAdd
BLAST
Chaini38 – 170133Secretion monitorPRO_0000031988Add
BLAST

Proteomic databases

PaxDbiP62395.
PRIDEiP62395.

Interactioni

Protein-protein interaction databases

BioGridi4261885. 277 interactions.
IntActiP62395. 1 interaction.
STRINGi511145.b0097.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2N62NMR-L150-166[»]
3JBVelectron microscopy3.32z150-166[»]
ProteinModelPortaliP62395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecM family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108SR7. Bacteria.
ENOG4111GJA. LUCA.
HOGENOMiHOG000267813.
KOiK13301.
OMAiSAWISQV.

Family and domain databases

HAMAPiMF_01332. SecM. 1 hit.
InterProiIPR009502. SecM.
[Graphical view]
PfamiPF06558. SecM. 1 hit.
[Graphical view]
PIRSFiPIRSF004572. SecM. 1 hit.
ProDomiPD089227. SecM. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGILTRWRQ FGKRYFWPHL LLGMVAASLG LPALSNAAEP NAPAKATTRN
60 70 80 90 100
HEPSAKVNFG QLALLEANTR RPNSNYSVDY WHQHAIRTVI RHLSFAMAPQ
110 120 130 140 150
TLPVAEESLP LQAQHLALLD TLSALLTQEG TPSEKGYRID YAHFTPQAKF
160 170
STPVWISQAQ GIRAGPQRLT
Length:170
Mass (Da):18,880
Last modified:July 5, 2004 - v1
Checksum:i5F5B3001056CAD22
GO

Sequence cautioni

The sequence AAA24618 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAA83850 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA38874 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19211 Genomic DNA. Translation: AAA83850.1. Different initiation.
M20791 Genomic DNA. Translation: AAA24618.1. Different initiation.
X55034 Genomic DNA. Translation: CAA38874.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73208.2.
AP009048 Genomic DNA. Translation: BAB96665.2.
PIRiA64732. QQECAA.
RefSeqiNP_414639.2. NC_000913.3.
WP_000014321.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73208; AAC73208; b0097.
BAB96665; BAB96665; BAB96665.
GeneIDi944831.
KEGGiecj:JW5007.
eco:b0097.
PATRICi32115299. VBIEscCol129921_0101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19211 Genomic DNA. Translation: AAA83850.1. Different initiation.
M20791 Genomic DNA. Translation: AAA24618.1. Different initiation.
X55034 Genomic DNA. Translation: CAA38874.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73208.2.
AP009048 Genomic DNA. Translation: BAB96665.2.
PIRiA64732. QQECAA.
RefSeqiNP_414639.2. NC_000913.3.
WP_000014321.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2N62NMR-L150-166[»]
3JBVelectron microscopy3.32z150-166[»]
ProteinModelPortaliP62395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261885. 277 interactions.
IntActiP62395. 1 interaction.
STRINGi511145.b0097.

Proteomic databases

PaxDbiP62395.
PRIDEiP62395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73208; AAC73208; b0097.
BAB96665; BAB96665; BAB96665.
GeneIDi944831.
KEGGiecj:JW5007.
eco:b0097.
PATRICi32115299. VBIEscCol129921_0101.

Organism-specific databases

EchoBASEiEB1079.
EcoGeneiEG11087. secM.

Phylogenomic databases

eggNOGiENOG4108SR7. Bacteria.
ENOG4111GJA. LUCA.
HOGENOMiHOG000267813.
KOiK13301.
OMAiSAWISQV.

Enzyme and pathway databases

BioCyciEcoCyc:EG11087-MONOMER.
ECOL316407:JW5007-MONOMER.

Miscellaneous databases

PROiP62395.

Family and domain databases

HAMAPiMF_01332. SecM. 1 hit.
InterProiIPR009502. SecM.
[Graphical view]
PfamiPF06558. SecM. 1 hit.
[Graphical view]
PIRSFiPIRSF004572. SecM. 1 hit.
ProDomiPD089227. SecM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiSECM_ECOLI
AccessioniPrimary (citable) accession number: P62395
Secondary accession number(s): P10409, P75641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.