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P62378

- RNP4_METMP

UniProt

P62378 - RNP4_METMP

Protein

Ribonuclease P protein component 4

Gene

rnp4

Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.1 PublicationUniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Kineticsi

    kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

    1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
    2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae1 Publication

    Temperature dependencei

    Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi65 – 651ZincUniRule annotation
    Metal bindingi68 – 681ZincUniRule annotation
    Metal bindingi94 – 941ZincUniRule annotation
    Metal bindingi97 – 971ZincUniRule annotation

    GO - Molecular functioni

    1. ribonuclease P activity Source: UniProtKB
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. tRNA 5'-leader removal Source: UniProtKB

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMMAR267377:GJ77-951-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease P protein component 4UniRule annotation (EC:3.1.26.5UniRule annotation)
    Short name:
    RNase P component 4UniRule annotation
    Alternative name(s):
    Rpp21UniRule annotation
    Gene namesi
    Name:rnp4UniRule annotation
    Ordered Locus Names:MMP0921
    OrganismiMethanococcus maripaludis (strain S2 / LL)
    Taxonomic identifieri267377 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
    ProteomesiUP000000590: Chromosome

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. ribonuclease P complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 110110Ribonuclease P protein component 4PRO_0000153855Add
    BLAST

    Interactioni

    Subunit structurei

    Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

    Protein-protein interaction databases

    STRINGi267377.MMP0921.

    Structurei

    3D structure databases

    ProteinModelPortaliP62378.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic/archaeal RNase P protein component 4 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2023.
    HOGENOMiHOG000230709.
    KOiK03540.
    OMAiPRKWKRR.

    Family and domain databases

    HAMAPiMF_00757. RNase_P_4.
    InterProiIPR016432. RNase_P_comp-4.
    IPR007175. Rpr2.
    [Graphical view]
    PANTHERiPTHR14742. PTHR14742. 1 hit.
    PfamiPF04032. Rpr2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004878. RNase_P_4. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P62378-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLKKKFLEK SKKVAEERIN ILMNLAEKES NSGKTERSKN YVLLGKKIAM    50
    RMRMPYPKEW KRRICKNCGS FLIYGKNARV RTKAKNYPHV VITCLECNSI 100
    TRIPIKTAKK 110
    Length:110
    Mass (Da):12,891
    Last modified:July 5, 2004 - v1
    Checksum:i46B9447B948D0F48
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF30477.1.
    RefSeqiNP_988041.1. NC_005791.1.
    WP_011170865.1. NC_005791.1.

    Genome annotation databases

    EnsemblBacteriaiCAF30477; CAF30477; MMP0921.
    GeneIDi2761316.
    KEGGimmp:MMP0921.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX950229 Genomic DNA. Translation: CAF30477.1 .
    RefSeqi NP_988041.1. NC_005791.1.
    WP_011170865.1. NC_005791.1.

    3D structure databases

    ProteinModelPortali P62378.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267377.MMP0921.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF30477 ; CAF30477 ; MMP0921 .
    GeneIDi 2761316.
    KEGGi mmp:MMP0921.

    Phylogenomic databases

    eggNOGi COG2023.
    HOGENOMi HOG000230709.
    KOi K03540.
    OMAi PRKWKRR.

    Enzyme and pathway databases

    BioCyci MMAR267377:GJ77-951-MONOMER.

    Family and domain databases

    HAMAPi MF_00757. RNase_P_4.
    InterProi IPR016432. RNase_P_comp-4.
    IPR007175. Rpr2.
    [Graphical view ]
    PANTHERi PTHR14742. PTHR14742. 1 hit.
    Pfami PF04032. Rpr2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004878. RNase_P_4. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S2 / LL.
    2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
      Strain: S2 / LL.

    Entry informationi

    Entry nameiRNP4_METMP
    AccessioniPrimary (citable) accession number: P62378
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3