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P62378

- RNP4_METMP

UniProt

P62378 - RNP4_METMP

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Protein
Ribonuclease P protein component 4
Gene
rnp4, MMP0921
Organism
Methanococcus maripaludis (strain S2 / LL)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Kineticsi

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

  1. KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae1 Publication
  2. KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae

Temperature dependencei

Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Zinc By similarity
Metal bindingi68 – 681Zinc By similarity
Metal bindingi94 – 941Zinc By similarity
Metal bindingi97 – 971Zinc By similarity

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB
  2. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR267377:GJ77-951-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease P protein component 4 (EC:3.1.26.5)
Short name:
RNase P component 4
Alternative name(s):
Rpp21
Gene namesi
Name:rnp4
Ordered Locus Names:MMP0921
OrganismiMethanococcus maripaludis (strain S2 / LL)
Taxonomic identifieri267377 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
ProteomesiUP000000590: Chromosome

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. ribonuclease P complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Ribonuclease P protein component 4UniRule annotation
PRO_0000153855Add
BLAST

Interactioni

Subunit structurei

Consists of a catalytic RNA component and at least 5 protein subunits.1 Publication

Protein-protein interaction databases

STRINGi267377.MMP0921.

Structurei

3D structure databases

ProteinModelPortaliP62378.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2023.
HOGENOMiHOG000230709.
KOiK03540.
OMAiPRKWKRR.

Family and domain databases

HAMAPiMF_00757. RNase_P_4.
InterProiIPR016432. RNase_P_comp-4.
IPR007175. Rpr2.
[Graphical view]
PANTHERiPTHR14742. PTHR14742. 1 hit.
PfamiPF04032. Rpr2. 1 hit.
[Graphical view]
PIRSFiPIRSF004878. RNase_P_4. 1 hit.

Sequencei

Sequence statusi: Complete.

P62378-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKKKFLEK SKKVAEERIN ILMNLAEKES NSGKTERSKN YVLLGKKIAM    50
RMRMPYPKEW KRRICKNCGS FLIYGKNARV RTKAKNYPHV VITCLECNSI 100
TRIPIKTAKK 110
Length:110
Mass (Da):12,891
Last modified:July 5, 2004 - v1
Checksum:i46B9447B948D0F48
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950229 Genomic DNA. Translation: CAF30477.1.
RefSeqiNP_988041.1. NC_005791.1.
WP_011170865.1. NC_005791.1.

Genome annotation databases

EnsemblBacteriaiCAF30477; CAF30477; MMP0921.
GeneIDi2761316.
KEGGimmp:MMP0921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX950229 Genomic DNA. Translation: CAF30477.1 .
RefSeqi NP_988041.1. NC_005791.1.
WP_011170865.1. NC_005791.1.

3D structure databases

ProteinModelPortali P62378.
ModBasei Search...

Protein-protein interaction databases

STRINGi 267377.MMP0921.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAF30477 ; CAF30477 ; MMP0921 .
GeneIDi 2761316.
KEGGi mmp:MMP0921.

Phylogenomic databases

eggNOGi COG2023.
HOGENOMi HOG000230709.
KOi K03540.
OMAi PRKWKRR.

Enzyme and pathway databases

BioCyci MMAR267377:GJ77-951-MONOMER.

Family and domain databases

HAMAPi MF_00757. RNase_P_4.
InterProi IPR016432. RNase_P_comp-4.
IPR007175. Rpr2.
[Graphical view ]
PANTHERi PTHR14742. PTHR14742. 1 hit.
Pfami PF04032. Rpr2. 1 hit.
[Graphical view ]
PIRSFi PIRSF004878. RNase_P_4. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S2 / LL.
  2. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: S2 / LL.

Entry informationi

Entry nameiRNP4_METMP
AccessioniPrimary (citable) accession number: P62378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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