Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P62378 (RNP4_METMP) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease P protein component 4

Short name=RNase P component 4
EC=3.1.26.5
Alternative name(s):
Rpp21
Gene names
Name:rnp4
Ordered Locus Names:MMP0921
OrganismMethanococcus maripaludis (strain S2 / LL) [Complete proteome] [HAMAP]
Taxonomic identifier267377 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length110 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ref.2

Catalytic activity

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00757

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00757

Subunit structure

Consists of a catalytic RNA component and at least 5 protein subunits. Ref.2

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the eukaryotic/archaeal RNase P protein component 4 family.

Biophysicochemical properties

Kinetic parameters:

kcat 10 min(-1) in absence of L7Ae, 63 min(-1) in presence of L7Ae. Kinetic parameters determined at 37 degrees Celsius.

KM=2.6 µM for pre-tRNA-Tyr in the absence of L7Ae Ref.2

KM=0.044 µM for pre-tRNA-Tyr in the presence of L7Ae

Temperature dependence:

Optimum temperature is 36-38 degrees Celsius in the absence of L7Ae, 48-50 degrees Celsius in presence of L7Ae.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 110110Ribonuclease P protein component 4 HAMAP-Rule MF_00757
PRO_0000153855

Sites

Metal binding651Zinc By similarity
Metal binding681Zinc By similarity
Metal binding941Zinc By similarity
Metal binding971Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
P62378 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 46B9447B948D0F48

FASTA11012,891
        10         20         30         40         50         60 
MKLKKKFLEK SKKVAEERIN ILMNLAEKES NSGKTERSKN YVLLGKKIAM RMRMPYPKEW 

        70         80         90        100        110 
KRRICKNCGS FLIYGKNARV RTKAKNYPHV VITCLECNSI TRIPIKTAKK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis."
Hendrickson E.L., Kaul R., Zhou Y., Bovee D., Chapman P., Chung J., Conway de Macario E., Dodsworth J.A., Gillett W., Graham D.E., Hackett M., Haydock A.K., Kang A., Land M.L., Levy R., Lie T.J., Major T.A., Moore B.C. expand/collapse author list , Porat I., Palmeiri A., Rouse G., Saenphimmachak C., Soell D., Van Dien S., Wang T., Whitman W.B., Xia Q., Zhang Y., Larimer F.W., Olson M.V., Leigh J.A.
J. Bacteriol. 186:6956-6969(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: S2 / LL.
[2]"Ribosomal protein L7Ae is a subunit of archaeal RNase P."
Cho I.M., Lai L.B., Susanti D., Mukhopadhyay B., Gopalan V.
Proc. Natl. Acad. Sci. U.S.A. 107:14573-14578(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION.
Strain: S2 / LL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX950229 Genomic DNA. Translation: CAF30477.1.
RefSeqNP_988041.1. NC_005791.1.

3D structure databases

ProteinModelPortalP62378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267377.MMP0921.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAF30477; CAF30477; MMP0921.
GeneID2761316.
KEGGmmp:MMP0921.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2023.
HOGENOMHOG000230709.
KOK03540.
OMAPRKWKRR.

Enzyme and pathway databases

BioCycMMAR267377:GJ77-951-MONOMER.

Family and domain databases

HAMAPMF_00757. RNase_P_4.
InterProIPR016432. RNase_P_comp-4.
IPR007175. Rpr2.
[Graphical view]
PANTHERPTHR14742. PTHR14742. 1 hit.
PfamPF04032. Rpr2. 1 hit.
[Graphical view]
PIRSFPIRSF004878. RNase_P_4. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNP4_METMP
AccessionPrimary (citable) accession number: P62378
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families