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Protein

Calcium-dependent protein kinase 1

Gene

CPK1

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Calcium-dependent kinase which may phosphorylate proteins of the host erythrocyte membranes.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Inhibited by calmodulin antagonists such as calmidazolium, trifluoperazine, N-[6-aminohexyl]-5-chloro-1-naphthalene-sulfonamide, and ophiobolin A (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85ATPPROSITE-ProRule annotation1
Active sitei191Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 70ATPPROSITE-ProRule annotation9
Calcium bindingi385 – 3961PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi429 – 4402PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi465 – 4763PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi499 – 5104PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein autophosphorylation Source: GO_Central
  • protein phosphorylation Source: GeneDB
  • response to drug Source: GeneDB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 4889.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 1 (EC:2.7.11.1)
Gene namesi
Name:CPK1
Synonyms:CDPK1
ORF Names:PFB0815w
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0217500.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity
  • Note: In the parasite and on erythrocytic membrane at a lower level.By similarity

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: GeneDB

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1908387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000858342 – 524Calcium-dependent protein kinase 1Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1

Post-translational modificationi

May be myristoylated; myristoylation may target it to different subcellular compartments.By similarity
Autophosphorylated in vitro.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

PTM databases

SwissPalmiP62344.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1208081. 9 interactors.
IntActiP62344. 8 interactors.
MINTiMINT-1544436.

Structurei

3D structure databases

ProteinModelPortaliP62344.
SMRiP62344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 325Protein kinasePROSITE-ProRule annotationAdd BLAST270
Domaini372 – 407EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini416 – 451EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini452 – 487EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini486 – 521EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233030.
InParanoidiP62344.
KOiK13412.
OMAiEVAIMSS.
PhylomeDBiP62344.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCSQSSNVK DFKTRRSKFT NGNNYGKSGN NKNSEDLAIN PGMYVRKKEG
60 70 80 90 100
KIGESYFKVR KLGSGAYGEV LLCREKHGHG EKAIKVIKKS QFDKMKYSIT
110 120 130 140 150
NKIECDDKIH EEIYNEISLL KSLDHPNIIK LFDVFEDKKY FYLVTEFYEG
160 170 180 190 200
GELFEQIINR HKFDECDAAN IMKQILSGIC YLHKHNIVHR DIKPENILLE
210 220 230 240 250
NKHSLLNIKI VDFGLSSFFS KDNKLRDRLG TAYYIAPEVL RKKYNEKCDV
260 270 280 290 300
WSCGVILYIL LCGYPPFGGQ NDQDIIKKVE KGKYYFDFND WKNISEEAKE
310 320 330 340 350
LIKLMLTYDY NKRITAKEAL NSKWIKKYAN NINKSDQKTL CGALSNMRKF
360 370 380 390 400
EGSQKLAQAA ILFIGSKLTT LEERKELTDI FKKLDKNGDG QLDKKELIEG
410 420 430 440 450
YNILRSFKNE LGELKNVEEE VDNILKEVDF DKNGYIEYSE FISVCMDKQI
460 470 480 490 500
LFSEERLRDA FNLFDTDKSG KITKEELANL FGLTSISEQM WNEVLGEADK
510 520
NKDNMIDFDE FVNMMHKICD NKSS
Length:524
Mass (Da):60,800
Last modified:January 23, 2007 - v2
Checksum:iD2A45E1579D2C951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001362 Genomic DNA. Translation: AAC71952.1.
PIRiA45472.
RefSeqiXP_001349680.1. XM_001349644.1.

Genome annotation databases

GeneDBiPF3D7_0217500.1:pep.
GeneIDi812762.
KEGGipfa:PFB0815w.

Similar proteinsi

Entry informationi

Entry nameiCDPK1_PLAF7
AccessioniPrimary (citable) accession number: P62344
Secondary accession number(s): Q27731
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families