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Protein

26S proteasome regulatory subunit 10B

Gene

Psmc6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC6 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 181ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 family signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-MMU-8939902. Regulation of RUNX2 expression and activity.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-8948751. Regulation of PTEN stability and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 10B
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT4
Proteasome 26S subunit ATPase 6
Proteasome subunit p42
Gene namesi
Name:Psmc6
Synonyms:Sug2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1914339. Psmc6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847331 – 38926S proteasome regulatory subunit 10BAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72N6-acetyllysineBy similarity1
Modified residuei206N6-acetyllysineCombined sources1
Modified residuei244PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62334.
PaxDbiP62334.
PeptideAtlasiP62334.
PRIDEiP62334.

2D gel databases

REPRODUCTION-2DPAGEiIPI00125971.
P62334.

PTM databases

iPTMnetiP62334.
PhosphoSitePlusiP62334.
SwissPalmiP62334.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021832.
ExpressionAtlasiP62334. baseline and differential.
GenevisibleiP62334. MM.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC6 and few additional components. Interacts with PAAF1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211932. 2 interactors.
IntActiP62334. 19 interactors.
MINTiMINT-1869834.
STRINGi10090.ENSMUSP00000022380.

Structurei

3D structure databases

ProteinModelPortaliP62334.
SMRiP62334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0651. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074826.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62334.
KOiK03064.
OMAiQPCVIFM.
OrthoDBiEOG091G096W.
PhylomeDBiP62334.
TreeFamiTF106229.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45-like.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR035263. PSMC6.
PANTHERiPTHR23073:SF59. PTHR23073:SF59. 1 hit.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

P62334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL
60 70 80 90 100
QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL
110 120 130 140 150
DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE
160 170 180 190 200
LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS
210 220 230 240 250
SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD
260 270 280 290 300
REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH
310 320 330 340 350
IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA
360 370 380
GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV
Length:389
Mass (Da):44,173
Last modified:July 5, 2004 - v1
Checksum:iB26421295742CACD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144E → D in BAB29293 (PubMed:16141072).Curated1
Sequence conflicti222A → V in BAB29293 (PubMed:16141072).Curated1
Sequence conflicti241R → P in AAH43044 (PubMed:15489334).Curated1
Sequence conflicti281R → S in BAB29293 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012174 mRNA. Translation: BAB28078.1.
AK014354 mRNA. Translation: BAB29293.1.
AK144728 mRNA. Translation: BAE26034.1.
AK166961 mRNA. Translation: BAE39144.1.
BC043044 mRNA. Translation: AAH43044.1.
BC057997 mRNA. Translation: AAH57997.1.
CCDSiCCDS26974.1.
RefSeqiNP_080235.2. NM_025959.3.
UniGeneiMm.18472.

Genome annotation databases

EnsembliENSMUST00000022380; ENSMUSP00000022380; ENSMUSG00000021832.
GeneIDi67089.
KEGGimmu:67089.
UCSCiuc007tgn.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPRS10_MOUSE
AccessioniPrimary (citable) accession number: P62334
Secondary accession number(s): P49719
, Q3TKK1, Q810A6, Q92524, Q9CXH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families