P62333 (PRS10_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 87.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 26S protease regulatory subunit 10B Alternative name(s): 26S proteasome AAA-ATPase subunit RPT4 Proteasome 26S subunit ATPase 6 Proteasome subunit p42 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 389 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| Subunit structure | Found in the multi-protein complexes: the 26S proteasome (formed from the 20S proteasome and PA700), and the modulator. PA700 consists of 28 subunits arranged to form a cylinder-shaped complex by four stacked rings, each containing seven subunits. Interacts with PAAF1. Ref.9 |
| Subcellular location | |
| Sequence similarities | Belongs to the AAA ATPase family. |
| Caution | Alternative initiation from an upstream conserved methionine cannot be fully excluded but is not experimentally supported while initiation from the displayed methionine is supported by Ref.10. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PSMC1 | P62191 | 3 | EBI-357669,EBI-357598 | |
| PSMC3 | P17980 | 5 | EBI-357669,EBI-359720 | |
| PSMC4 | P43686 | 3 | EBI-357669,EBI-743997 | |
| PSMD9 | O00233 | 4 | EBI-357669,EBI-750973 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 389 | 388 | 26S protease regulatory subunit 10B | PRO_0000084732 | |||||
Regions | |||||||||
| Nucleotide binding | 174 – 181 | 8 | ATP Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Ref.10 | ||||||
| Modified residue | 20 | 1 | N6-acetyllysine Ref.12 | ||||||
| Modified residue | 72 | 1 | N6-acetyllysine Ref.12 | ||||||
| Modified residue | 206 | 1 | N6-acetyllysine Ref.12 | ||||||
| Modified residue | 207 | 1 | Phosphotyrosine Ref.11 | ||||||
Experimental info | |||||||||
| Sequence conflict | 276 | 1 | I → T in BAA11338. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family." Fujiwara T., Watanabe T.K., Tanaka K., Slaughter C.A., Demartino G.N. FEBS Lett. 387:184-188(1996) [PubMed: 8674546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aorta. |
| [2] | "Human SUG2." Li Y., Benezra R. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Caudate nucleus. |
| [6] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder. |
| [8] | "Identification, purification, and characterization of a PA700-dependent activator of the proteasome." Demartino G.N., Proske R.J., Moomaw C.R., Strong A.A., Song X., Hisamatsu H., Tanaka K., Slaughter C.A. J. Biol. Chem. 271:3112-3118(1996) [PubMed: 8621709] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. |
| [9] | "Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases." Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B. Mol. Cell. Biol. 25:3842-3853(2005) [PubMed: 15831487] [Abstract] Cited for: INTERACTION WITH PAAF1. |
| [10] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney. |
| [11] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-207, MASS SPECTROMETRY. Tissue: Lung carcinoma. |
| [12] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-20; LYS-72 AND LYS-206, MASS SPECTROMETRY. |
| [13] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D78275 mRNA. Translation: BAA11338.1. AF006305 mRNA. Translation: AAB61616.1. BT006843 mRNA. Translation: AAP35489.1. CR456709 mRNA. Translation: CAG32990.1. AK313670 mRNA. Translation: BAG36422.1. CH471078 Genomic DNA. Translation: EAW65645.1. BC005390 mRNA. Translation: AAH05390.1. |
| IPI | IPI00926977. |
| PIR | S71316. |
| RefSeq | NP_002797.3. NM_002806.3. |
| UniGene | Hs.156171. |
3D structure databases | |
| ProteinModelPortal | P62333. |
| SMR | P62333. Positions 26-112, 122-381. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-38150N. |
| IntAct | P62333. 21 interactions. |
| MINT | MINT-5001174. |
| STRING | P62333. |
PTM databases | |
| PhosphoSite | P62333. |
Polymorphism databases | |
| DMDM | 51702772. |
2D gel databases | |
| REPRODUCTION-2DPAGE | IPI00021926. |
Proteomic databases | |
| PeptideAtlas | P62333. |
| PRIDE | P62333. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000216407; ENSP00000216407; ENSG00000100519. ENST00000445930; ENSP00000401802; ENSG00000100519. |
| GeneID | 5706. |
| KEGG | hsa:5706. |
| UCSC | uc001wzx.1. human. |
Organism-specific databases | |
| CTD | 5706. |
| GeneCards | GC14P053173. |
| H-InvDB | HIX0011661. HIX0188739. |
| HGNC | HGNC:9553. PSMC6. |
| MIM | 602708. gene. |
| neXtProt | NX_P62333. |
| PharmGKB | PA33898. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG07425. |
| GeneTree | ENSGT00550000074826. |
| HOGENOM | HBG724153. |
| HOVERGEN | HBG000109. |
| InParanoid | P62333. |
| OrthoDB | EOG408N84. |
| PhylomeDB | P62333. |
Enzyme and pathway databases | |
| Reactome | REACT_111102. Signal Transduction. REACT_111217. Metabolism. REACT_13505. Proteasome mediated degradation of PAK-2p34. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_6900. Immune System. |
Gene expression databases | |
| ArrayExpress | P62333. |
| Bgee | P62333. |
| CleanEx | HS_PSMC6. |
| Genevestigator | P62333. |
| GermOnline | ENSG00000100519. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005937. 26S_Psome_P45. IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view] |
| KO | K03064. |
| Pfam | PF00004. AAA. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01242. 26Sp45. 1 hit. |
| PROSITE | PS00674. AAA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 22170. |
| SOURCE | Search... |
Entry information
| Entry name | PRS10_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P62333 Secondary accession number(s): B2R975 Q92524 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with