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Protein

26S protease regulatory subunit 10B

Gene

PSMC6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi174 – 181ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • protein binding, bridging Source: UniProtKB
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS02101-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 10B
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT4
Proteasome 26S subunit ATPase 6
Proteasome subunit p42
Gene namesi
Name:PSMC6
Synonyms:SUG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:9553. PSMC6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • cytosolic proteasome complex Source: GO_Central
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nuclear proteasome complex Source: GO_Central
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • proteasome accessory complex Source: UniProtKB
  • proteasome complex Source: UniProtKB
  • proteasome regulatory particle, base subcomplex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5706.
OpenTargetsiENSG00000100519.
PharmGKBiPA33898.

Polymorphism and mutation databases

BioMutaiPSMC6.
DMDMi51702772.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847321 – 38926S protease regulatory subunit 10BAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72N6-acetyllysineCombined sources1
Modified residuei206N6-acetyllysineCombined sources1
Modified residuei244PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62333.
MaxQBiP62333.
PaxDbiP62333.
PeptideAtlasiP62333.
PRIDEiP62333.

2D gel databases

REPRODUCTION-2DPAGEIPI00021926.

PTM databases

iPTMnetiP62333.
PhosphoSitePlusiP62333.
SwissPalmiP62333.

Expressioni

Gene expression databases

BgeeiENSG00000100519.
CleanExiHS_PSMC6.
ExpressionAtlasiP62333. baseline and differential.
GenevisibleiP62333. HS.

Organism-specific databases

HPAiHPA042823.
HPA061191.

Interactioni

Subunit structurei

Found in the multi-protein complexes: the 26S proteasome (formed from the 20S proteasome and PA700), and the modulator. PA700 consists of 28 subunits arranged to form a cylinder-shaped complex by four stacked rings, each containing seven subunits. Interacts with PAAF1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-357669,EBI-357669
C1orf216Q8TAB53EBI-357669,EBI-747505
CCDC146Q8IYE0-23EBI-357669,EBI-10247802
CYB5R2Q6BCY43EBI-357669,EBI-744761
PSMC1P621914EBI-357669,EBI-357598
PSMC3P1798014EBI-357669,EBI-359720
PSMC4P436863EBI-357669,EBI-743997
PSMC5P6219510EBI-357669,EBI-357745
PSMD9O0023310EBI-357669,EBI-750973
SDCBPO005607EBI-357669,EBI-727004

GO - Molecular functioni

  • protein binding, bridging Source: UniProtKB
  • TBP-class protein binding Source: GO_Central

Protein-protein interaction databases

BioGridi111679. 118 interactors.
DIPiDIP-38150N.
IntActiP62333. 57 interactors.
MINTiMINT-5001174.
STRINGi9606.ENSP00000401802.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50L1-389[»]
5GJRelectron microscopy3.50L/z1-389[»]
5L4Gelectron microscopy4.02L1-389[»]
5T0Celectron microscopy3.80AE/BE1-389[»]
ProteinModelPortaliP62333.
SMRiP62333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0651. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074826.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62333.
KOiK03064.
PhylomeDBiP62333.
TreeFamiTF106229.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPRDKALQ DYRKKLLEHK EIDGRLKELR EQLKELTKQY EKSENDLKAL
60 70 80 90 100
QSVGQIVGEV LKQLTEEKFI VKATNGPRYV VGCRRQLDKS KLKPGTRVAL
110 120 130 140 150
DMTTLTIMRY LPREVDPLVY NMSHEDPGNV SYSEIGGLSE QIRELREVIE
160 170 180 190 200
LPLTNPELFQ RVGIIPPKGC LLYGPPGTGK TLLARAVASQ LDCNFLKVVS
210 220 230 240 250
SSIVDKYIGE SARLIREMFN YARDHQPCII FMDEIDAIGG RRFSEGTSAD
260 270 280 290 300
REIQRTLMEL LNQMDGFDTL HRVKMIMATN RPDTLDPALL RPGRLDRKIH
310 320 330 340 350
IDLPNEQARL DILKIHAGPI TKHGEIDYEA IVKLSDGFNG ADLRNVCTEA
360 370 380
GMFAIRADHD FVVQEDFMKA VRKVADSKKL ESKLDYKPV
Length:389
Mass (Da):44,173
Last modified:July 5, 2004 - v1
Checksum:iB26421295742CACD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276I → T in BAA11338 (PubMed:8674546).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78275 mRNA. Translation: BAA11338.1.
AF006305 mRNA. Translation: AAB61616.1.
BT006843 mRNA. Translation: AAP35489.1.
CR456709 mRNA. Translation: CAG32990.1.
AK313670 mRNA. Translation: BAG36422.1.
CH471078 Genomic DNA. Translation: EAW65645.1.
BC005390 mRNA. Translation: AAH05390.1.
PIRiS71316.
RefSeqiNP_002797.3. NM_002806.3.
UniGeneiHs.156171.

Genome annotation databases

EnsembliENST00000606149; ENSP00000475721; ENSG00000100519.
GeneIDi5706.
KEGGihsa:5706.
UCSCiuc059bor.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78275 mRNA. Translation: BAA11338.1.
AF006305 mRNA. Translation: AAB61616.1.
BT006843 mRNA. Translation: AAP35489.1.
CR456709 mRNA. Translation: CAG32990.1.
AK313670 mRNA. Translation: BAG36422.1.
CH471078 Genomic DNA. Translation: EAW65645.1.
BC005390 mRNA. Translation: AAH05390.1.
PIRiS71316.
RefSeqiNP_002797.3. NM_002806.3.
UniGeneiHs.156171.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50L1-389[»]
5GJRelectron microscopy3.50L/z1-389[»]
5L4Gelectron microscopy4.02L1-389[»]
5T0Celectron microscopy3.80AE/BE1-389[»]
ProteinModelPortaliP62333.
SMRiP62333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111679. 118 interactors.
DIPiDIP-38150N.
IntActiP62333. 57 interactors.
MINTiMINT-5001174.
STRINGi9606.ENSP00000401802.

PTM databases

iPTMnetiP62333.
PhosphoSitePlusiP62333.
SwissPalmiP62333.

Polymorphism and mutation databases

BioMutaiPSMC6.
DMDMi51702772.

2D gel databases

REPRODUCTION-2DPAGEIPI00021926.

Proteomic databases

EPDiP62333.
MaxQBiP62333.
PaxDbiP62333.
PeptideAtlasiP62333.
PRIDEiP62333.

Protocols and materials databases

DNASUi5706.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000606149; ENSP00000475721; ENSG00000100519.
GeneIDi5706.
KEGGihsa:5706.
UCSCiuc059bor.1. human.

Organism-specific databases

CTDi5706.
DisGeNETi5706.
GeneCardsiPSMC6.
H-InvDBHIX0011661.
HGNCiHGNC:9553. PSMC6.
HPAiHPA042823.
HPA061191.
MIMi602708. gene.
neXtProtiNX_P62333.
OpenTargetsiENSG00000100519.
PharmGKBiPA33898.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0651. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074826.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62333.
KOiK03064.
PhylomeDBiP62333.
TreeFamiTF106229.

Enzyme and pathway databases

BioCyciZFISH:HS02101-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GeneWikiiPSMC6.
GenomeRNAii5706.
PROiP62333.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100519.
CleanExiHS_PSMC6.
ExpressionAtlasiP62333. baseline and differential.
GenevisibleiP62333. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRS10_HUMAN
AccessioniPrimary (citable) accession number: P62333
Secondary accession number(s): B2R975
, P49719, Q6IBU3, Q92524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Alternative initiation from an upstream conserved methionine cannot be fully excluded but is not experimentally supported while initiation from the displayed methionine is supported by PubMed:17323924.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.