Reviewed,
UniProtKB/Swiss-Prot P62333 (PRS10_HUMAN)
Last modified
November 25, 2008.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 26S protease regulatory subunit S10B Alternative name(s): Proteasome 26S subunit ATPase 6 Proteasome subunit p42 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 389 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| Subunit structure | Found in the multi-protein complexes: the 26S proteasome (formed from the 20S proteasome and PA700), and the modulator. PA700 consists of 28 subunits arranged to form a cylinder-shaped complex by four stacked rings, each containing seven subunits. Interacts with PAAF1. |
| Subcellular location | |
| Sequence similarities | Belongs to the AAA ATPase family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PAAF1 | Q9BRP4 | 1 | EBI-357669,EBI-1056358 | |
| PSMD10 | O75832 | 1 | EBI-357669,EBI-752185 | |
| PSMD6 | Q15008 | 1 | EBI-357669,EBI-359701 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 389 | 388 | 26S protease regulatory subunit S10B | PRO_0000084732 | |||||
Regions | |||||||||
| Nucleotide binding | 174 – 181 | 8 | ATP Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine | ||||||
| Modified residue | 207 | 1 | Phosphotyrosine | ||||||
Experimental info | |||||||||
| Sequence conflict | 276 | 1 | I → T in BAA11338. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning of p42, a shared subunit of two proteasome regulatory proteins, reveals a novel member of the AAA protein family." Fujiwara T., Watanabe T.K., Tanaka K., Slaughter C.A., Demartino G.N. FEBS Lett. 387:184-188(1996) [PubMed: 8674546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aorta. |
| [2] | "Human SUG2." Li Y., Benezra R. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder. |
| [6] | "Identification, purification, and characterization of a PA700-dependent activator of the proteasome." Demartino G.N., Proske R.J., Moomaw C.R., Strong A.A., Song X., Hisamatsu H., Tanaka K., Slaughter C.A. J. Biol. Chem. 271:3112-3118(1996) [PubMed: 8621709] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. |
| [7] | "Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases." Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B. Mol. Cell. Biol. 25:3842-3853(2005) [PubMed: 15831487] [Abstract] Cited for: INTERACTION WITH PAAF1. |
| [8] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. |
| [9] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-207, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| D78275 mRNA. Translation: BAA11338.1. AF006305 mRNA. Translation: AAB61616.1. BT006843 mRNA. Translation: AAP35489.1. CR456709 mRNA. Translation: CAG32990.1. BC005390 mRNA. Translation: AAH05390.1. | |
| PIR | S71316. |
| RefSeq | NP_002797.3. |
| UniGene | Hs.156171 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IM2 based on UniProtKB P43773. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P62333. |
PTM databases | |
| PhosphoSite | P62333. |
2-D gel databases | |
| REPRODUCTION-2DPAGE | IPI00021926. |
Proteomic databases | |
| PeptideAtlas | P62333. |
Genome annotation databases | |
| Ensembl | ENSG00000100519. Homo sapiens. [Contig view] |
| GeneID | 5706. |
| KEGG | hsa:5706. |
Organism-specific databases | |
| H-InvDB | HIX0011661. HIX0034665. |
| HGNC | HGNC:9553. PSMC6. |
| MIM | 602708. gene. |
| PharmGKB | PA33898. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | P62333. |
| HOVERGEN | P62333. |
Enzyme and pathway databases | |
| Reactome | REACT_11045. Signaling by Wnt. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| CleanEx | HS_PSMC6. |
| GermOnline | ENSG00000100519. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005937. 26S_Psome_P45. IPR003593. AAA+_ATPase_core. IPR003959. AAA_ATPase_core. IPR003960. AAA_ATPase_CS. [Graphical view] |
| Pfam | PF00004. AAA. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01242. 26Sp45. 1 hit. |
| PROSITE | PS00674. AAA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P62333. |
| NextBio | 22170. |
| SOURCE | Search... |
Entry information
| Entry name | PRS10_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P62333 Secondary accession number(s): P49719, Q6IBU3, Q92524 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


