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Protein

ADP-ribosylation factor 6

Gene

Arf6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.1 Publication

Enzyme regulationi

Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi122 – 1254GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, ER-Golgi transport, Neurogenesis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 6
Gene namesi
Name:Arf6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621279. Arf6.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: Ensembl
  • early endosome Source: RGD
  • endocytic vesicle Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • filopodium membrane Source: UniProtKB-SubCell
  • focal adhesion Source: Ensembl
  • Golgi apparatus Source: UniProtKB-SubCell
  • myelin sheath Source: Ensembl
  • plasma membrane Source: RGD
  • recycling endosome Source: RGD
  • recycling endosome membrane Source: UniProtKB
  • ruffle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 175174ADP-ribosylation factor 6PRO_0000207402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP62332.
PRIDEiP62332.

PTM databases

iPTMnetiP62332.
PhosphoSiteiP62332.

Expressioni

Gene expression databases

GenevisibleiP62332. RN.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Interacts with MICALL1.1 Publication

Protein-protein interaction databases

BioGridi249405. 3 interactions.
IntActiP62332. 1 interaction.
MINTiMINT-4577977.
STRINGi10116.ENSRNOP00000006355.

Structurei

3D structure databases

ProteinModelPortaliP62332.
SMRiP62332. Positions 12-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0071. Eukaryota.
ENOG410XP1U. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP62332.
KOiK07941.
OMAiRFNVWDV.
OrthoDBiEOG7F5133.
PhylomeDBiP62332.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE
60 70 80 90 100
TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR
110 120 130 140 150
QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY
160 170
VQPSCATSGD GLYEGLTWLT SNYKS
Length:175
Mass (Da):20,082
Last modified:January 23, 2007 - v2
Checksum:i49E38E59AEA52B98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12385 mRNA. Translation: AAA40690.1.
BC091146 mRNA. Translation: AAH91146.1.
RefSeqiNP_077066.1. NM_024152.2.
UniGeneiRn.6993.

Genome annotation databases

EnsembliENSRNOT00000006355; ENSRNOP00000006355; ENSRNOG00000004791.
GeneIDi79121.
KEGGirno:79121.
UCSCiRGD:621279. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12385 mRNA. Translation: AAA40690.1.
BC091146 mRNA. Translation: AAH91146.1.
RefSeqiNP_077066.1. NM_024152.2.
UniGeneiRn.6993.

3D structure databases

ProteinModelPortaliP62332.
SMRiP62332. Positions 12-174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249405. 3 interactions.
IntActiP62332. 1 interaction.
MINTiMINT-4577977.
STRINGi10116.ENSRNOP00000006355.

PTM databases

iPTMnetiP62332.
PhosphoSiteiP62332.

Proteomic databases

PaxDbiP62332.
PRIDEiP62332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006355; ENSRNOP00000006355; ENSRNOG00000004791.
GeneIDi79121.
KEGGirno:79121.
UCSCiRGD:621279. rat.

Organism-specific databases

CTDi382.
RGDi621279. Arf6.

Phylogenomic databases

eggNOGiKOG0071. Eukaryota.
ENOG410XP1U. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP62332.
KOiK07941.
OMAiRFNVWDV.
OrthoDBiEOG7F5133.
PhylomeDBiP62332.
TreeFamiTF300808.

Miscellaneous databases

NextBioi614542.
PROiP62332.

Gene expression databases

GenevisibleiP62332. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interspecies relationships among ADP-ribosylation factors (ARFs): evidence of evolutionary pressure to maintain individual identities."
    Price S.R., Nightingale M.S., Tsuchiya M., Moss J., Vaughan M.
    Mol. Cell. Biochem. 159:15-23(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  3. "Identification of a new Pyk2 target protein with Arf-GAP activity."
    Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.
    Mol. Cell. Biol. 19:2338-2350(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ASAP2.
  4. "ARF6 and EFA6A regulate the development and maintenance of dendritic spines."
    Choi S., Ko J., Lee J.R., Lee H.W., Kim K., Chung H.S., Kim H., Kim E.
    J. Neurosci. 26:4811-4819(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS REGULATOR OF DENDRITIC SPINE DEVELOPMENT.

Entry informationi

Entry nameiARF6_RAT
AccessioniPrimary (citable) accession number: P62332
Secondary accession number(s): P26438, Q5BKA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.