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Protein

ADP-ribosylation factor 6

Gene

Arf6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.1 Publication

Enzyme regulationi

Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi122 – 125GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation, ER-Golgi transport, Neurogenesis, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-8854214. TBC/RABGAPs.
R-RNO-8856828. Clathrin-mediated endocytosis.
R-RNO-8875656. MET receptor recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 6
Gene namesi
Name:Arf6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621279. Arf6.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Cell membrane By similarity; Lipid-anchor By similarity
  • Endosome membrane By similarity; Lipid-anchor By similarity
  • Recycling endosome membrane By similarity; Lipid-anchor By similarity
  • Cell projectionfilopodium membrane By similarity; Lipid-anchor By similarity
  • Cell projectionruffle By similarity
  • Cleavage furrow By similarity
  • MidbodyMidbody ring By similarity
  • Golgi apparatus By similarity

  • Note: Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body. Recruitement to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1. After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (By similarity). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles. Myristoylation is required for proper localization to membranes (By similarity).By similarity

GO - Cellular componenti

  • cell cortex Source: Ensembl
  • cleavage furrow Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: RGD
  • endocytic vesicle Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • filopodium membrane Source: UniProtKB-SubCell
  • Flemming body Source: UniProtKB
  • focal adhesion Source: Ensembl
  • Golgi apparatus Source: UniProtKB-SubCell
  • myelin sheath Source: Ensembl
  • plasma membrane Source: RGD
  • recycling endosome Source: RGD
  • recycling endosome membrane Source: UniProtKB
  • ruffle Source: RGD

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002074022 – 175ADP-ribosylation factor 6Add BLAST174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP62332.
PRIDEiP62332.

PTM databases

iPTMnetiP62332.
PhosphoSitePlusiP62332.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004791.
GenevisibleiP62332. RN.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Interacts with MICALL1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249405. 4 interactors.
IntActiP62332. 1 interactor.
MINTiMINT-4577977.
STRINGi10116.ENSRNOP00000006355.

Structurei

3D structure databases

ProteinModelPortaliP62332.
SMRiP62332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0071. Eukaryota.
ENOG410XP1U. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP62332.
KOiK07941.
OMAiIQDREMK.
OrthoDBiEOG091G0OXD.
PhylomeDBiP62332.
TreeFamiTF300808.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
PfamiView protein in Pfam
PF00025. Arf. 1 hit.
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51417. ARF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE
60 70 80 90 100
TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR
110 120 130 140 150
QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY
160 170
VQPSCATSGD GLYEGLTWLT SNYKS
Length:175
Mass (Da):20,082
Last modified:January 23, 2007 - v2
Checksum:i49E38E59AEA52B98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12385 mRNA. Translation: AAA40690.1.
BC091146 mRNA. Translation: AAH91146.1.
RefSeqiNP_077066.1. NM_024152.2.
UniGeneiRn.6993.

Genome annotation databases

EnsembliENSRNOT00000006355; ENSRNOP00000006355; ENSRNOG00000004791.
GeneIDi79121.
KEGGirno:79121.
UCSCiRGD:621279. rat.

Similar proteinsi

Entry informationi

Entry nameiARF6_RAT
AccessioniPrimary (citable) accession number: P62332
Secondary accession number(s): P26438, Q5BKA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families