Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P62331

- ARF6_MOUSE

UniProt

P62331 - ARF6_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

ADP-ribosylation factor 6

Gene

Arf6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.3 Publications

Enzyme regulationi

Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 By similarity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 286GTP1 Publication
Nucleotide bindingi41 – 444GTPBy similarity
Nucleotide bindingi63 – 675GTP1 Publication
Nucleotide bindingi122 – 1254GTP1 Publication
Nucleotide bindingi155 – 1562GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: BHF-UCL
  2. GTP binding Source: BHF-UCL

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cortical actin cytoskeleton organization Source: Ensembl
  4. establishment of epithelial cell polarity Source: MGI
  5. GTP catabolic process Source: GOC
  6. hepatocyte apoptotic process Source: MGI
  7. liver development Source: MGI
  8. myeloid cell apoptotic process Source: MGI
  9. positive regulation of actin filament polymerization Source: Ensembl
  10. positive regulation of establishment of protein localization to plasma membrane Source: BHF-UCL
  11. protein localization to cell surface Source: BHF-UCL
  12. protein localization to endosome Source: UniProtKB
  13. protein transport Source: MGI
  14. regulation of dendritic spine development Source: UniProtKB
  15. regulation of filopodium assembly Source: Ensembl
  16. regulation of Rac protein signal transduction Source: Ensembl
  17. regulation of toll-like receptor 4 signaling pathway Source: BHF-UCL
  18. ruffle organization Source: Ensembl
  19. small GTPase mediated signal transduction Source: InterPro
  20. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Differentiation, ER-Golgi transport, Neurogenesis, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 6
Gene namesi
Name:Arf6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:99435. Arf6.

Subcellular locationi

Golgi apparatus By similarity. Cell membrane By similarity; Lipid-anchor By similarity. Endosome membrane By similarity; Lipid-anchor By similarity. Recycling endosome membrane By similarity; Lipid-anchor By similarity. Cell projectionfilopodium membrane By similarity; Lipid-anchor By similarity. Midbody. Cytoplasm. Cleavage furrow
Note: Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles By similarity. Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23.By similarity

GO - Cellular componenti

  1. cell cortex Source: Ensembl
  2. cytoplasm Source: MGI
  3. early endosome Source: Ensembl
  4. endocytic vesicle Source: UniProtKB
  5. endosome Source: UniProtKB
  6. extracellular vesicular exosome Source: Ensembl
  7. filopodium membrane Source: Ensembl
  8. Golgi apparatus Source: UniProtKB-KW
  9. plasma membrane Source: MGI
  10. recycling endosome membrane Source: UniProtKB
  11. ruffle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271T → N: Loss of activity. 1 Publication
Mutagenesisi67 – 671Q → L: Probable constitutively active mutant that prevents EHD1 localization to endosome membranes. 2 Publications
Mutagenesisi76 – 761H → A: Slightly impaired interaction with KIF23. Abolishes interaction with GGA1, SPAG9 and RAB11FIP4. 1 Publication
Mutagenesisi77 – 771Y → A: Loss of interaction with KIF23, GGA1, SPAG9 and RAB11FIP4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 175174ADP-ribosylation factor 6PRO_0000207401Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP62331.
PaxDbiP62331.
PRIDEiP62331.

PTM databases

PhosphoSiteiP62331.

Expressioni

Gene expression databases

BgeeiP62331.
CleanExiMM_ARF6.
ExpressionAtlasiP62331. baseline and differential.
GenevestigatoriP62331.

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Interacts with MICALL1. Interacts with CYTH3 By similarity. Interacts with KIF23, forming heterodimers and heterotetramers. Interacts with GGA1, SPAG9 and RAB11FIP4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GGA1Q9UJY52EBI-988682,EBI-447141From a different organism.
KIF23Q0224110EBI-988682,EBI-306852From a different organism.
Mapk8ip3Q9ESN98EBI-988682,EBI-301496
RAB11FIP4Q86YS32EBI-988682,EBI-949727From a different organism.
SPAG9O602712EBI-988682,EBI-1023301From a different organism.
Spag9Q58A6510EBI-988682,EBI-6530207
Tjap1Q9DCD53EBI-988682,EBI-775733

Protein-protein interaction databases

BioGridi198189. 3 interactions.
IntActiP62331. 12 interactions.
MINTiMINT-1866703.
STRINGi10090.ENSMUSP00000055862.

Structurei

Secondary structure

1
175
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 197Combined sources
Helixi26 – 3510Combined sources
Beta strandi45 – 5410Combined sources
Beta strandi57 – 648Combined sources
Turni68 – 703Combined sources
Helixi71 – 777Combined sources
Beta strandi82 – 898Combined sources
Helixi96 – 10712Combined sources
Helixi110 – 1123Combined sources
Beta strandi116 – 1227Combined sources
Helixi132 – 1387Combined sources
Beta strandi149 – 1535Combined sources
Turni156 – 1594Combined sources
Helixi162 – 17110Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VHXX-ray2.81A/C/E/G13-175[»]
ProteinModelPortaliP62331.
SMRiP62331. Positions 12-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiP62331.
KOiK07941.
OMAiRFNVWDV.
OrthoDBiEOG7F5133.
PhylomeDBiP62331.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62331-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE
60 70 80 90 100
TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR
110 120 130 140 150
QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY
160 170
VQPSCATSGD GLYEGLTWLT SNYKS
Length:175
Mass (Da):20,082
Last modified:January 23, 2007 - v2
Checksum:i49E38E59AEA52B98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87903 mRNA. Translation: BAA13495.1.
BC003478 mRNA. Translation: AAH03478.1.
BC083112 mRNA. Translation: AAH83112.1.
CCDSiCCDS25952.1.
PIRiJC4950.
RefSeqiNP_031507.1. NM_007481.3.
UniGeneiMm.27308.

Genome annotation databases

EnsembliENSMUST00000050063; ENSMUSP00000055862; ENSMUSG00000044147.
GeneIDi11845.
KEGGimmu:11845.
UCSCiuc007nsj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87903 mRNA. Translation: BAA13495.1 .
BC003478 mRNA. Translation: AAH03478.1 .
BC083112 mRNA. Translation: AAH83112.1 .
CCDSi CCDS25952.1.
PIRi JC4950.
RefSeqi NP_031507.1. NM_007481.3.
UniGenei Mm.27308.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VHX X-ray 2.81 A/C/E/G 13-175 [» ]
ProteinModelPortali P62331.
SMRi P62331. Positions 12-174.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198189. 3 interactions.
IntActi P62331. 12 interactions.
MINTi MINT-1866703.
STRINGi 10090.ENSMUSP00000055862.

Chemistry

ChEMBLi CHEMBL1075274.

PTM databases

PhosphoSitei P62331.

Proteomic databases

MaxQBi P62331.
PaxDbi P62331.
PRIDEi P62331.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000050063 ; ENSMUSP00000055862 ; ENSMUSG00000044147 .
GeneIDi 11845.
KEGGi mmu:11845.
UCSCi uc007nsj.1. mouse.

Organism-specific databases

CTDi 382.
MGIi MGI:99435. Arf6.

Phylogenomic databases

eggNOGi COG1100.
HOGENOMi HOG000163691.
HOVERGENi HBG002073.
InParanoidi P62331.
KOi K07941.
OMAi RFNVWDV.
OrthoDBi EOG7F5133.
PhylomeDBi P62331.
TreeFami TF300808.

Miscellaneous databases

NextBioi 1599.
PROi P62331.
SOURCEi Search...

Gene expression databases

Bgeei P62331.
CleanExi MM_ARF6.
ExpressionAtlasi P62331. baseline and differential.
Genevestigatori P62331.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6)."
    Hosaka M., Toda K., Takatsu H., Torii S., Murakami K., Nakayama K.
    J. Biochem. 120:813-819(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: ICR.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "The small GTPase ADP-ribosylation factor 6 negatively regulates dendritic spine formation."
    Miyazaki H., Yamazaki M., Watanabe H., Maehama T., Yokozeki T., Kanaho Y.
    FEBS Lett. 579:6834-6838(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS REGULATOR OF DENDRITIC SPINE DEVELOPMENT.
  4. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
    Kobayashi H., Fukuda M.
    J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEURITE OUTGROWTH, MUTAGENESIS OF GLN-67.
  5. "Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
    Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
    EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 13-175 IN COMPLEX WITH GTP AND KIF23, INTERACTION WITH KIF23; RAB11FIP4; GGA1 AND SPAG9, FUNCTION, MUTAGENESIS OF THR-27; GLN-67; HIS-76 AND TYR-77, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiARF6_MOUSE
AccessioniPrimary (citable) accession number: P62331
Secondary accession number(s): P26438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3