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P62331

- ARF6_MOUSE

UniProt

P62331 - ARF6_MOUSE

Protein

ADP-ribosylation factor 6

Gene

Arf6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling. Required for normal completion of mitotic cytokinesis. May also modulate vesicle budding and uncoating within the Golgi apparatus. Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension.3 Publications

    Enzyme regulationi

    Activation is generally mediated by guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by GTPases activating protein (GAP). Inactivated by ACAP1 and ACAP2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi23 – 286GTP1 Publication
    Nucleotide bindingi41 – 444GTPBy similarity
    Nucleotide bindingi63 – 675GTP1 Publication
    Nucleotide bindingi122 – 1254GTP1 Publication
    Nucleotide bindingi155 – 1562GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: BHF-UCL
    2. GTP binding Source: BHF-UCL
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. cortical actin cytoskeleton organization Source: Ensembl
    4. establishment of epithelial cell polarity Source: MGI
    5. GTP catabolic process Source: GOC
    6. hepatocyte apoptotic process Source: MGI
    7. liver development Source: MGI
    8. myeloid cell apoptotic process Source: MGI
    9. positive regulation of actin filament polymerization Source: Ensembl
    10. positive regulation of establishment of protein localization to plasma membrane Source: BHF-UCL
    11. protein localization to cell surface Source: BHF-UCL
    12. protein localization to endosome Source: UniProtKB
    13. protein transport Source: MGI
    14. regulation of dendritic spine development Source: UniProtKB
    15. regulation of filopodium assembly Source: Ensembl
    16. regulation of Rac protein signal transduction Source: Ensembl
    17. regulation of toll-like receptor 4 signaling pathway Source: BHF-UCL
    18. ruffle organization Source: Ensembl
    19. small GTPase mediated signal transduction Source: InterPro
    20. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Differentiation, ER-Golgi transport, Neurogenesis, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor 6
    Gene namesi
    Name:Arf6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:99435. Arf6.

    Subcellular locationi

    Golgi apparatus By similarity. Cell membrane By similarity; Lipid-anchor By similarity. Endosome membrane By similarity; Lipid-anchor By similarity. Recycling endosome membrane By similarity; Lipid-anchor By similarity. Cell projectionfilopodium membrane By similarity; Lipid-anchor By similarity. Midbody. Cytoplasm. Cleavage furrow
    Note: Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles By similarity. Distributed throughout the cytoplasm during metaphase. Transiently detected at the ingressing cleavage furrow during mitotic cytokinesis. Recruited to the midbody at later stages of cytokinesis; this requires interaction with KIF23.By similarity

    GO - Cellular componenti

    1. cell cortex Source: Ensembl
    2. cleavage furrow Source: UniProtKB-SubCell
    3. cytoplasm Source: MGI
    4. early endosome Source: Ensembl
    5. endocytic vesicle Source: UniProtKB
    6. endosome Source: UniProtKB
    7. filopodium membrane Source: UniProtKB-SubCell
    8. Golgi apparatus Source: UniProtKB-SubCell
    9. midbody Source: UniProtKB-SubCell
    10. plasma membrane Source: MGI
    11. recycling endosome membrane Source: UniProtKB
    12. ruffle Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271T → N: Loss of activity. 1 Publication
    Mutagenesisi67 – 671Q → L: Probable constitutively active mutant that prevents EHD1 localization to endosome membranes. 2 Publications
    Mutagenesisi76 – 761H → A: Slightly impaired interaction with KIF23. Abolishes interaction with GGA1, SPAG9 and RAB11FIP4. 1 Publication
    Mutagenesisi77 – 771Y → A: Loss of interaction with KIF23, GGA1, SPAG9 and RAB11FIP4. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 175174ADP-ribosylation factor 6PRO_0000207401Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    MaxQBiP62331.
    PaxDbiP62331.
    PRIDEiP62331.

    PTM databases

    PhosphoSiteiP62331.

    Expressioni

    Gene expression databases

    ArrayExpressiP62331.
    BgeeiP62331.
    CleanExiMM_ARF6.
    GenevestigatoriP62331.

    Interactioni

    Subunit structurei

    Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Interacts with MICALL1. Interacts with CYTH3 By similarity. Interacts with KIF23, forming heterodimers and heterotetramers. Interacts with GGA1, SPAG9 and RAB11FIP4.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GGA1Q9UJY52EBI-988682,EBI-447141From a different organism.
    KIF23Q0224110EBI-988682,EBI-306852From a different organism.
    Mapk8ip3Q9ESN98EBI-988682,EBI-301496
    RAB11FIP4Q86YS32EBI-988682,EBI-949727From a different organism.
    SPAG9O602712EBI-988682,EBI-1023301From a different organism.
    Spag9Q58A6510EBI-988682,EBI-6530207
    Tjap1Q9DCD53EBI-988682,EBI-775733

    Protein-protein interaction databases

    BioGridi198189. 3 interactions.
    IntActiP62331. 12 interactions.
    MINTiMINT-1866703.
    STRINGi10090.ENSMUSP00000055862.

    Structurei

    Secondary structure

    1
    175
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 197
    Helixi26 – 3510
    Beta strandi45 – 5410
    Beta strandi57 – 648
    Turni68 – 703
    Helixi71 – 777
    Beta strandi82 – 898
    Helixi96 – 10712
    Helixi110 – 1123
    Beta strandi116 – 1227
    Helixi132 – 1387
    Beta strandi149 – 1535
    Turni156 – 1594
    Helixi162 – 17110

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VHXX-ray2.81A/C/E/G13-175[»]
    ProteinModelPortaliP62331.
    SMRiP62331. Positions 12-174.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Arf family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000163691.
    HOVERGENiHBG002073.
    InParanoidiP62331.
    KOiK07941.
    OMAiRFNVWDV.
    OrthoDBiEOG7F5133.
    PhylomeDBiP62331.
    TreeFamiTF300808.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view]
    PfamiPF00025. Arf. 1 hit.
    [Graphical view]
    PRINTSiPR00328. SAR1GTPBP.
    SMARTiSM00177. ARF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51417. ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62331-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE    50
    TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR 100
    QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY 150
    VQPSCATSGD GLYEGLTWLT SNYKS 175
    Length:175
    Mass (Da):20,082
    Last modified:January 23, 2007 - v2
    Checksum:i49E38E59AEA52B98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87903 mRNA. Translation: BAA13495.1.
    BC003478 mRNA. Translation: AAH03478.1.
    BC083112 mRNA. Translation: AAH83112.1.
    CCDSiCCDS25952.1.
    PIRiJC4950.
    RefSeqiNP_031507.1. NM_007481.3.
    UniGeneiMm.27308.

    Genome annotation databases

    EnsembliENSMUST00000050063; ENSMUSP00000055862; ENSMUSG00000044147.
    GeneIDi11845.
    KEGGimmu:11845.
    UCSCiuc007nsj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87903 mRNA. Translation: BAA13495.1 .
    BC003478 mRNA. Translation: AAH03478.1 .
    BC083112 mRNA. Translation: AAH83112.1 .
    CCDSi CCDS25952.1.
    PIRi JC4950.
    RefSeqi NP_031507.1. NM_007481.3.
    UniGenei Mm.27308.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VHX X-ray 2.81 A/C/E/G 13-175 [» ]
    ProteinModelPortali P62331.
    SMRi P62331. Positions 12-174.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198189. 3 interactions.
    IntActi P62331. 12 interactions.
    MINTi MINT-1866703.
    STRINGi 10090.ENSMUSP00000055862.

    Chemistry

    ChEMBLi CHEMBL1075274.

    PTM databases

    PhosphoSitei P62331.

    Proteomic databases

    MaxQBi P62331.
    PaxDbi P62331.
    PRIDEi P62331.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000050063 ; ENSMUSP00000055862 ; ENSMUSG00000044147 .
    GeneIDi 11845.
    KEGGi mmu:11845.
    UCSCi uc007nsj.1. mouse.

    Organism-specific databases

    CTDi 382.
    MGIi MGI:99435. Arf6.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000163691.
    HOVERGENi HBG002073.
    InParanoidi P62331.
    KOi K07941.
    OMAi RFNVWDV.
    OrthoDBi EOG7F5133.
    PhylomeDBi P62331.
    TreeFami TF300808.

    Miscellaneous databases

    NextBioi 1599.
    PROi P62331.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62331.
    Bgeei P62331.
    CleanExi MM_ARF6.
    Genevestigatori P62331.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view ]
    Pfami PF00025. Arf. 1 hit.
    [Graphical view ]
    PRINTSi PR00328. SAR1GTPBP.
    SMARTi SM00177. ARF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51417. ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6)."
      Hosaka M., Toda K., Takatsu H., Torii S., Murakami K., Nakayama K.
      J. Biochem. 120:813-819(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: ICR.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "The small GTPase ADP-ribosylation factor 6 negatively regulates dendritic spine formation."
      Miyazaki H., Yamazaki M., Watanabe H., Maehama T., Yokozeki T., Kanaho Y.
      FEBS Lett. 579:6834-6838(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS REGULATOR OF DENDRITIC SPINE DEVELOPMENT.
    4. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
      Kobayashi H., Fukuda M.
      J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEURITE OUTGROWTH, MUTAGENESIS OF GLN-67.
    5. "Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis."
      Makyio H., Ohgi M., Takei T., Takahashi S., Takatsu H., Katoh Y., Hanai A., Ueda T., Kanaho Y., Xie Y., Shin H.W., Kamikubo H., Kataoka M., Kawasaki M., Kato R., Wakatsuki S., Nakayama K.
      EMBO J. 31:2590-2603(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.81 ANGSTROMS) OF 13-175 IN COMPLEX WITH GTP AND KIF23, INTERACTION WITH KIF23; RAB11FIP4; GGA1 AND SPAG9, FUNCTION, MUTAGENESIS OF THR-27; GLN-67; HIS-76 AND TYR-77, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiARF6_MOUSE
    AccessioniPrimary (citable) accession number: P62331
    Secondary accession number(s): P26438
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3