ADP-ribosylation factor 6 - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P62330 (ARF6_HUMAN)

Last modified July 7, 2009. Version 66. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
ADP-ribosylation factor 6

Gene names

Name:

ARF6

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Ref.5 Ref.10
Subunit structure	Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus. Ref.5
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

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Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Golgi apparatus
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell adhesion Traceable author statement. Source: UniProtKB cell motion Traceable author statement. Source: UniProtKB cortical actin cytoskeleton organization Inferred from mutant phenotype. Source: UniProtKB negative regulation of receptor-mediated endocytosis Traceable author statement. Source: UniProtKB positive regulation of actin filament polymerization Inferred from mutant phenotype. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of Rac protein signal transduction Inferred from direct assay. Source: UniProtKB ruffle organization Inferred from direct assay. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Traceable author statement. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell cortex Inferred from direct assay. Source: UniProtKB endosome Traceable author statement. Source: UniProtKB membrane fraction Traceable author statement. Source: ProtInc plasma membrane Inferred from direct assay. Source: UniProtKB ruffle Inferred from direct assay. Source: UniProtKB
Molecular function	GTP binding Traceable author statement. Source: UniProtKB GTPase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
ARFIP2	P53365	1	EBI-638181,EBI-638194
ATP6V1C1	P21283	4	EBI-638181,EBI-988663
DHPS	P49366	1	EBI-638181,EBI-741925
DYNC1H1	Q14204	1	EBI-638181,EBI-356015
EIF6	P56537	1	EBI-638181,EBI-372243
EPPK1	P58107	1	EBI-638181,EBI-297954
GART	P22102	1	EBI-638181,EBI-356297
IQGAP1	P46940	1	EBI-638181,EBI-297509
LRPPRC	P42704	1	EBI-638181,EBI-1050853
PABPC4	Q13310	1	EBI-638181,EBI-372844
YARS	P54577	1	EBI-638181,EBI-1048893

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 175

174

ADP-ribosylation factor 6

PRO_0000207400

Regions

Nucleotide binding

20 – 27

GTP By similarity

Nucleotide binding

63 – 67

GTP By similarity

Nucleotide binding

122 – 125

GTP By similarity

Amino acid modifications

Lipidation

N-myristoyl glycine

Experimental info

Mutagenesis

G → A: Fails to associate with membranes. Ref.5

Secondary structure

175

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P62330-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 49E38E59AEA52B98
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FASTA

175

20,082

        10         20         30         40         50         60 
MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN 

        70         80         90        100        110        120 
VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF 

       130        140        150        160        170 
ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells." Tsuchiya M., Price S.R., Tsai S.-C., Moss J., Vaughan M. J. Biol. Chem. 266:2772-2777(1991) [PubMed: 1993656] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z. Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed: 9653160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin and Uterus.
[5]	"Myristoylation is required for the intracellular localization and endocytic function of ARF6." D'Souza-Schorey C., Stahl P.D. Exp. Cell Res. 221:153-159(1995) [PubMed: 7589240] [Abstract] Cited for: MYRISTOYLATION AT GLY-2, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2.
[6]	"ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma." Krauss M., Kinuta M., Wenk M.R., De Camilli P., Takei K., Haucke V. J. Cell Biol. 162:113-124(2003) [PubMed: 12847086] [Abstract] Cited for: INTERACTION WITH PIP5K1C.
[7]	"Requirement of phosphatidylinositol-4,5-bisphosphate for HERC1-mediated guanine nucleotide release from ARF proteins." Garcia-Gonzalo F.R., Bartrons R., Ventura F., Rosa J.L. FEBS Lett. 579:343-348(2005) [PubMed: 15642342] [Abstract] Cited for: INTERACTION WITH HERC1.
[8]	"Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics." Dubois T., Paleotti O., Mironov A.A. Jr., Fraisier V., Stradal T.E.B., De Matteis M.A., Franco M., Chavrier P. Nat. Cell Biol. 7:353-364(2005) [PubMed: 15793564] [Abstract] Cited for: INTERACTION WITH ARHGAP21.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"The structural GDP/GTP cycle of human Arf6." Pasqualato S., Menetrey J., Franco M., Cherfils J. EMBO Rep. 2:234-238(2001) [PubMed: 11266366] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), FUNCTION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M57763 mRNA. Translation: AAA90928.1.
AF047432 mRNA. Translation: AAC39877.1.
AF493885 mRNA. Translation: AAM12599.1.
BC002952 mRNA. No translation available.
BC008918 mRNA. Translation: AAH08918.1.

IPI

IPI00215920.

PIR

B23741.

RefSeq

NP_001654.1.

UniGene

Hs.525330
Hs.712645

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E0S	X-ray	2.28	A	2-175	[»]
2A5D	X-ray	1.80	A	2-174	[»]
2A5F	X-ray	2.02	A	2-174	[»]
2A5G	X-ray	2.66	A	2-174	[»]
2BAO	NMR	-	A	2-11	[»]
2BAU	NMR	-	A	2-11	[»]
2J5X	X-ray	2.80	A/B	1-175	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P62330. 112 interactions.

Proteomic databases

PeptideAtlas

P62330.

PRIDE

P62330.

Genome annotation databases

Ensembl

ENSG00000165527. Homo sapiens. [Contig view]

GeneID

382.

KEGG

hsa:382.

UCSC

uc001wxg.2. human.

Organism-specific databases

GeneCards

GC14P049429.

H-InvDB

HIX0011635.

HGNC

HGNC:659. ARF6.

HPA

CAB002778.

MIM

600464. gene.

PharmGKB

PA24942.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P62330.

HOVERGEN

P62330.

OMA

P62330. WLTSNHK.

Enzyme and pathway databases

Pathway_Interaction_DB

arf6downstreampathway. Arf6 downstream pathway.
arf6cyclingpathway. Arf6 signaling events.
arf6_traffickingpathway. Arf6 trafficking events.
pi3kcipathway. Class I PI3K signaling events.
a4b1_paxdep_pathway. Paxillin-dependent events mediated by a4b1.
a4b1_paxindep_pathway. Paxillin-independent events mediated by a4b1 and a4b7.

Gene expression databases

ArrayExpress

P62330.

Bgee

P62330.

CleanEx

HS_ARF6.

GermOnline

ENSG00000165527. Homo sapiens.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

1599.

SOURCE

Search...

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Entry information

Entry name

ARF6_HUMAN

Accession

Primary (citable) accession number: P62330
Secondary accession number(s): P26438

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents