P62329 (TYB4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymosin beta-4
Short name=T beta 4

Cleaved into the following chain:

1. Hematopoietic system regulatory peptide
   Alternative name(s):
   Seraspenide

Gene names

Name:	Tmsb4x
Synonyms:	Thyb4, Tmsb4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	44 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization By similarity. Ref.2 Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase By similarity. Ref.2
Subunit structure	Interacts with SERPINB1. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X By similarity.
Subcellular location	Cytoplasm › cytoskeleton.
Tissue specificity	Originally found in thymus but it is widely distributed in many tissues. Ref.5
Induction	By NGF and fibroblast growth factors. Ref.6
Sequence similarities	Belongs to the thymosin beta family.
Sequence caution	The sequence AAA42246.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from electronic annotation. Source: InterPro osteoblast differentiation Inferred from expression pattern Ref.1. Source: RGD sequestering of actin monomers Traceable author statement PubMed 11251199. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 44	43	Thymosin beta-4		PRO_0000045927
	Peptide	2 – 5	4	Hematopoietic system regulatory peptide By similarity		PRO_0000034301
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	23	1	Phosphothreonine Ref.7
	Modified residue	26	1	N6-acetyllysine By similarity
	Modified residue	39	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	8	1	A → V in AAA42245. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P62329 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 440C6158482DAAD0	FASTA	44	5,053
	10 20 30 40 MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Thymosin beta 4 is expressed in ROS 17/2.8 osteosarcoma cells in a regulated manner." Atkinson M.J., Freeman M.W., Kronenberg H.M. Mol. Endocrinol. 4:69-74(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and sequence analysis of cDNA for rat spleen thymosin beta 4." Wodnar-Filipowicz A., Gubler U., Furuichi Y., Richardson M., Nowoswiat E.F., Poonian M.S., Horecker B.L. Proc. Natl. Acad. Sci. U.S.A. 81:2295-2297(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]	"Sequence of a cloned 523-bp cDNA for thymosin beta 4." Goodall G.J., Richardson M., Furuichi Y., Wodnar-Filippwicz A., Horecker B.L. Arch. Biochem. Biophys. 236:445-447(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[5]	"Thymosin beta 4: a ubiquitous peptide in rat and mouse tissues." Hannappel E., Xu G.J., Morgan J., Hempstead J., Horecker B.L. Proc. Natl. Acad. Sci. U.S.A. 79:2172-2175(1982) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Identification and characterization of mRNAs regulated by nerve growth factor in PC12 cells." Leonard D.G., Ziff E.B., Greene L.A. Mol. Cell. Biol. 7:3156-3167(1987) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-23, MASS SPECTROMETRY. Tissue: Renal collecting duct.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34043 mRNA. Translation: AAA42062.1. K01334 mRNA. Translation: AAA42245.1. M26759 mRNA. Translation: AAA42246.1. Different initiation. BC058137 mRNA. Translation: AAH58137.1.
IPI	IPI00230925.
PIR	TNRTB4. A01522. I52084.
RefSeq	NP_112398.1. NM_031136.1.
UniGene	Rn.201192. Rn.203155. Rn.230178. Rn.2605.
3D structure databases
ProteinModelPortal	P62329.
SMR	P62329. Positions 2-42.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000040574.
PTM databases
PhosphoSite	P62329.
Proteomic databases
PaxDb	P62329.
PRIDE	P62329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000071708; ENSRNOP00000066062; ENSRNOG00000047931.
GeneID	81814.
KEGG	rno:81814.
Organism-specific databases
CTD	7114.
RGD	621622. Tmsb4x.
Phylogenomic databases
eggNOG	NOG126035.
GeneTree	ENSGT00390000007040.
HOVERGEN	HBG012534.
InParanoid	P62329.
KO	K05764.
OrthoDB	EOG4HDSWB.
Gene expression databases
ArrayExpress	P62329.
Genevestigator	P62329.
GermOnline	ENSRNOG00000031043. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.5.520. 1 hit.
InterPro	IPR001152. Thymosin_b4. IPR016323. Thymosin_b4_chordata. [Graphical view]
PANTHER	PTHR12021. PTHR12021. 1 hit.
Pfam	PF01290. Thymosin. 1 hit. [Graphical view]
PIRSF	PIRSF001828. Thymosin_beta. 1 hit.
ProDom	PD005116. Thymosin_b4. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00152. THY. 1 hit. [Graphical view]
PROSITE	PS00500. THYMOSIN_B4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615711.

Entry information

Entry name

TYB4_RAT

Accession

Primary (citable) accession number: P62329
Secondary accession number(s): P01253 , P01254, Q0P5V6, Q63576

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program