Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P62328

- TYB4_HUMAN

UniProt

P62328 - TYB4_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Thymosin beta-4

Gene

TMSB4X

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.By similarity
Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. blood coagulation Source: Reactome
  3. platelet activation Source: Reactome
  4. platelet degranulation Source: Reactome
  5. sequestering of actin monomers Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymosin beta-4
Short name:
T beta-4
Alternative name(s):
Fx
Cleaved into the following chain:
Alternative name(s):
Seraspenide
Gene namesi
Name:TMSB4X
Synonyms:TB4X, THYB4, TMSB4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:11881. TMSB4X.

Subcellular locationi

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. extracellular region Source: Reactome
  3. platelet alpha granule lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi12 – 121K → P: Very weak actin binding; no inhibition of actin polymerization.
Mutagenesisi16 – 161S → A: Binds actin 2.5-fold less than wild-type; little change in inhibition of actin polymerization.
Mutagenesisi16 – 161S → AS: Very weak actin binding; no inhibition of actin polymerization.
Mutagenesisi18 – 181L → A or P: Very weak actin binding; no inhibition of actin polymerization.

Organism-specific databases

PharmGKBiPA36581.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 4443Thymosin beta-4PRO_0000045920Add
BLAST
Peptidei2 – 54Hematopoietic system regulatory peptideBy similarityPRO_0000034295

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei26 – 261N6-acetyllysine1 Publication
Modified residuei39 – 391N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP62328.
PaxDbiP62328.
PRIDEiP62328.

2D gel databases

DOSAC-COBS-2DPAGEP62328.

PTM databases

PhosphoSiteiP62328.

Expressioni

Tissue specificityi

Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.1 Publication

Inductioni

By alpha interferons. Decreased levels in THP-1 cells after treatment with recombinant interferon-lambda.2 Publications

Gene expression databases

BgeeiP62328.
CleanExiHS_TMSB4X.
ExpressionAtlasiP62328. baseline.
GenevestigatoriP62328.

Organism-specific databases

HPAiCAB033806.

Interactioni

Subunit structurei

Interacts with SERPINB1 (By similarity). Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P4069216EBI-712598,EBI-744248

Protein-protein interaction databases

BioGridi112969. 13 interactions.
IntActiP62328. 22 interactions.
MINTiMINT-6542280.
STRINGi9606.ENSP00000370007.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UY5model-T2-44[»]
3TU5X-ray3.00B21-44[»]
DisProtiDP00357.
ProteinModelPortaliP62328.
SMRiP62328. Positions 2-42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymosin beta family.Curated

Phylogenomic databases

eggNOGiNOG126035.
GeneTreeiENSGT00390000007040.
HOVERGENiHBG012534.
InParanoidiP62328.
KOiK05764.
OMAiQTENRIC.
OrthoDBiEOG7TJ3MT.
PhylomeDBiP62328.

Family and domain databases

Gene3Di1.20.5.520. 1 hit.
InterProiIPR001152. Thymosin_b4.
IPR016323. Thymosin_b4_metazoa.
[Graphical view]
PANTHERiPTHR12021. PTHR12021. 1 hit.
PfamiPF01290. Thymosin. 1 hit.
[Graphical view]
PIRSFiPIRSF001828. Thymosin_beta. 1 hit.
ProDomiPD005116. Thymosin_b4. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00152. THY. 1 hit.
[Graphical view]
PROSITEiPS00500. THYMOSIN_B4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62328-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40 
MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES
Length:44
Mass (Da):5,053
Last modified:January 23, 2007 - v2
Checksum:i440C6158482DAAD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17733 mRNA. Translation: AAA36745.1.
AJ295158 Genomic DNA. Translation: CAC43317.1.
BT007090 mRNA. Translation: AAP35753.1.
X02493 mRNA. Translation: CAA26323.1.
CCDSiCCDS35202.1.
PIRiI56000. A38682.
RefSeqiNP_066932.1. NM_021109.3.
UniGeneiHs.437277.
Hs.703237.

Genome annotation databases

EnsembliENST00000380633; ENSP00000370007; ENSG00000205542.
ENST00000380635; ENSP00000370009; ENSG00000205542.
ENST00000380636; ENSP00000370010; ENSG00000205542.
ENST00000451311; ENSP00000414376; ENSG00000205542.
GeneIDi7114.
KEGGihsa:7114.
UCSCiuc004cvf.3. human.

Polymorphism databases

DMDMi78103211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17733 mRNA. Translation: AAA36745.1 .
AJ295158 Genomic DNA. Translation: CAC43317.1 .
BT007090 mRNA. Translation: AAP35753.1 .
X02493 mRNA. Translation: CAA26323.1 .
CCDSi CCDS35202.1.
PIRi I56000. A38682.
RefSeqi NP_066932.1. NM_021109.3.
UniGenei Hs.437277.
Hs.703237.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1UY5 model - T 2-44 [» ]
3TU5 X-ray 3.00 B 21-44 [» ]
DisProti DP00357.
ProteinModelPortali P62328.
SMRi P62328. Positions 2-42.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112969. 13 interactions.
IntActi P62328. 22 interactions.
MINTi MINT-6542280.
STRINGi 9606.ENSP00000370007.

PTM databases

PhosphoSitei P62328.

Polymorphism databases

DMDMi 78103211.

2D gel databases

DOSAC-COBS-2DPAGE P62328.

Proteomic databases

MaxQBi P62328.
PaxDbi P62328.
PRIDEi P62328.

Protocols and materials databases

DNASUi 7114.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000380633 ; ENSP00000370007 ; ENSG00000205542 .
ENST00000380635 ; ENSP00000370009 ; ENSG00000205542 .
ENST00000380636 ; ENSP00000370010 ; ENSG00000205542 .
ENST00000451311 ; ENSP00000414376 ; ENSG00000205542 .
GeneIDi 7114.
KEGGi hsa:7114.
UCSCi uc004cvf.3. human.

Organism-specific databases

CTDi 7114.
GeneCardsi GC0XP012993.
HGNCi HGNC:11881. TMSB4X.
HPAi CAB033806.
MIMi 300159. gene.
neXtProti NX_P62328.
PharmGKBi PA36581.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG126035.
GeneTreei ENSGT00390000007040.
HOVERGENi HBG012534.
InParanoidi P62328.
KOi K05764.
OMAi QTENRIC.
OrthoDBi EOG7TJ3MT.
PhylomeDBi P62328.

Miscellaneous databases

ChiTaRSi TMSB4X. human.
GeneWikii Thymosin_beta-4.
GenomeRNAii 7114.
NextBioi 27851.
PROi P62328.
SOURCEi Search...

Gene expression databases

Bgeei P62328.
CleanExi HS_TMSB4X.
ExpressionAtlasi P62328. baseline.
Genevestigatori P62328.

Family and domain databases

Gene3Di 1.20.5.520. 1 hit.
InterProi IPR001152. Thymosin_b4.
IPR016323. Thymosin_b4_metazoa.
[Graphical view ]
PANTHERi PTHR12021. PTHR12021. 1 hit.
Pfami PF01290. Thymosin. 1 hit.
[Graphical view ]
PIRSFi PIRSF001828. Thymosin_beta. 1 hit.
ProDomi PD005116. Thymosin_b4. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00152. THY. 1 hit.
[Graphical view ]
PROSITEi PS00500. THYMOSIN_B4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential expression of the human thymosin-beta 4 gene in lymphocytes, macrophages, and granulocytes."
    Gondo H., Kudo J., White J.W., Barr C., Selvanayagam P., Saunders G.F.
    J. Immunol. 139:3840-3848(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, TISSUE SPECIFICITY.
    Tissue: Peripheral blood leukocyte.
  2. "Molecular cloning and structural characterization of the functional human thymosin beta4 gene."
    Yang S.P., Lee H.J., Su Y.
    Mol. Cell. Biochem. 272:97-105(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Thymosin beta 4 and Fx, an actin-sequestering peptide, are indistinguishable."
    Safer D., Elzinga M., Nachmias V.T.
    J. Biol. Chem. 266:4029-4032(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-44, INTERACTION WITH ACTIN, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2.
  5. "Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells."
    Friedman R.L., Manly S.P., McMahon M., Kerr I.M., Stark G.R.
    Cell 38:745-755(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-44, INDUCTION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-26 AND LYS-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structural requirements for thymosin beta4 in its contact with actin. An NMR-analysis of thymosin beta4 mutants in solution and correlation with their biological activity."
    Simenel C., Van Troys M., Vandekerckhove J., Ampe C., Delepierre M.
    Eur. J. Biochem. 267:3530-3538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF WILD-TYPE AND OF MUTANTS LYS-12; SER-16 AND LEU-18 IN COMPLEX WITH ACTIN.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 21-44 IN COMPLEX WITH ACTA1; GSN AND COBL, SUBUNIT.

Entry informationi

Entry nameiTYB4_HUMAN
AccessioniPrimary (citable) accession number: P62328
Secondary accession number(s): P01253
, P01254, Q546P5, Q63576, Q9UE55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3