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P62308

- RUXG_HUMAN

UniProt

P62308 - RUXG_HUMAN

Protein

Small nuclear ribonucleoprotein G

Gene

SNRPG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing.2 Publications

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. gene expression Source: Reactome
    2. histone mRNA metabolic process Source: Reactome
    3. mRNA 3'-end processing Source: Reactome
    4. mRNA splicing, via spliceosome Source: UniProtKB
    5. ncRNA metabolic process Source: Reactome
    6. RNA metabolic process Source: Reactome
    7. RNA splicing Source: UniProtKB
    8. spliceosomal complex assembly Source: UniProtKB
    9. spliceosomal snRNP assembly Source: UniProtKB
    10. termination of RNA polymerase II transcription Source: Reactome
    11. transcription from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_11066. snRNP Assembly.
    REACT_1364. SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_185. SLBP independent Processing of Histone Pre-mRNAs.
    REACT_467. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Small nuclear ribonucleoprotein G
    Short name:
    snRNP-G
    Alternative name(s):
    Sm protein G
    Short name:
    Sm-G
    Short name:
    SmG
    Gene namesi
    Name:SNRPG
    Synonyms:PBSCG
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:11163. SNRPG.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Nucleus 1 Publication
    Note: SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes.

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. methylosome Source: UniProtKB
    4. nucleoplasm Source: Reactome
    5. small nuclear ribonucleoprotein complex Source: UniProtKB
    6. SMN-Sm protein complex Source: UniProtKB
    7. spliceosomal complex Source: UniProtKB
    8. U12-type spliceosomal complex Source: UniProtKB
    9. U1 snRNP Source: UniProtKB
    10. U4 snRNP Source: UniProtKB
    11. U7 snRNP Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36004.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7676Small nuclear ribonucleoprotein GPRO_0000125545Add
    BLAST

    Proteomic databases

    MaxQBiP62308.
    PeptideAtlasiP62308.
    PRIDEiP62308.

    PTM databases

    PhosphoSiteiP62308.

    Expressioni

    Gene expression databases

    BgeeiP62308.
    CleanExiHS_SNRPG.
    GenevestigatoriP62308.

    Interactioni

    Subunit structurei

    U1 snRNP is for instance composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG; catalyzes core snRNPs assembly. Forms a 6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics additional Sm proteins and which is unable to assemble into the core snRNP. Interacts with TACC1.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNRPEP623044EBI-624585,EBI-348082
    TACC1O75410-15EBI-624585,EBI-624252

    Protein-protein interaction databases

    BioGridi112521. 40 interactions.
    DIPiDIP-34627N.
    IntActiP62308. 23 interactions.
    MINTiMINT-1527983.
    STRINGi9606.ENSP00000272348.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 94
    Helixi10 – 123
    Beta strandi16 – 216
    Turni22 – 243
    Beta strandi25 – 3410
    Beta strandi40 – 478
    Beta strandi57 – 626
    Beta strandi64 – 718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VU2X-ray3.104/H/P/X/f/n/v1-76[»]
    1VU3X-ray3.10H/P/X/f/n/v1-76[»]
    2Y9AX-ray3.60G/N/U1-76[»]
    2Y9BX-ray3.60G/N/U1-76[»]
    2Y9CX-ray3.60G/N/U1-76[»]
    2Y9DX-ray3.60G/N/U1-76[»]
    3CW1X-ray5.493/4/5/G1-76[»]
    3PGWX-ray4.40G/J1-76[»]
    3S6NX-ray2.50G1-76[»]
    4F77X-ray3.104/H/P/X/f/n/v1-76[»]
    4F7UX-ray1.90G/J1-76[»]
    ProteinModelPortaliP62308.
    SMRiP62308. Positions 7-74.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62308.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the snRNP Sm proteins family.Curated

    Phylogenomic databases

    HOVERGENiHBG000513.
    InParanoidiP62308.
    KOiK11099.
    OMAiRSAYVCR.
    OrthoDBiEOG7S221K.
    TreeFamiTF315099.

    Family and domain databases

    InterProiIPR010920. LSM_dom.
    IPR001163. Ribonucl_LSM.
    IPR006649. Ribonucl_LSM_euk/arc.
    [Graphical view]
    PfamiPF01423. LSM. 1 hit.
    [Graphical view]
    SMARTiSM00651. Sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF50182. SSF50182. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P62308-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT   50
    SGQQNNIGMV VIRGNSIIML EALERV 76
    Length:76
    Mass (Da):8,496
    Last modified:July 5, 2004 - v1
    Checksum:i623302BF6BE758FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85373 mRNA. Translation: CAA59689.1.
    CR456918 mRNA. Translation: CAG33199.1.
    AC079338 Genomic DNA. Translation: AAX81996.1.
    CH471053 Genomic DNA. Translation: EAW99817.1.
    CH471053 Genomic DNA. Translation: EAW99818.1.
    BC000070 mRNA. Translation: AAH00070.1.
    BC022432 mRNA. Translation: AAH22432.1.
    BC066302 mRNA. Translation: AAH66302.1.
    BC070166 mRNA. Translation: AAH70166.1.
    BC071880 mRNA. Translation: AAH71880.1.
    BC106055 mRNA. Translation: AAI06056.1.
    CCDSiCCDS1903.1.
    PIRiS55054.
    RefSeqiNP_003087.1. NM_003096.2.
    UniGeneiHs.465167.
    Hs.516076.
    Hs.631639.
    Hs.654528.

    Genome annotation databases

    EnsembliENST00000272348; ENSP00000272348; ENSG00000143977.
    GeneIDi6637.
    KEGGihsa:6637.
    UCSCiuc002sgp.3. human.

    Polymorphism databases

    DMDMi59800216.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85373 mRNA. Translation: CAA59689.1 .
    CR456918 mRNA. Translation: CAG33199.1 .
    AC079338 Genomic DNA. Translation: AAX81996.1 .
    CH471053 Genomic DNA. Translation: EAW99817.1 .
    CH471053 Genomic DNA. Translation: EAW99818.1 .
    BC000070 mRNA. Translation: AAH00070.1 .
    BC022432 mRNA. Translation: AAH22432.1 .
    BC066302 mRNA. Translation: AAH66302.1 .
    BC070166 mRNA. Translation: AAH70166.1 .
    BC071880 mRNA. Translation: AAH71880.1 .
    BC106055 mRNA. Translation: AAI06056.1 .
    CCDSi CCDS1903.1.
    PIRi S55054.
    RefSeqi NP_003087.1. NM_003096.2.
    UniGenei Hs.465167.
    Hs.516076.
    Hs.631639.
    Hs.654528.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VU2 X-ray 3.10 4/H/P/X/f/n/v 1-76 [» ]
    1VU3 X-ray 3.10 H/P/X/f/n/v 1-76 [» ]
    2Y9A X-ray 3.60 G/N/U 1-76 [» ]
    2Y9B X-ray 3.60 G/N/U 1-76 [» ]
    2Y9C X-ray 3.60 G/N/U 1-76 [» ]
    2Y9D X-ray 3.60 G/N/U 1-76 [» ]
    3CW1 X-ray 5.49 3/4/5/G 1-76 [» ]
    3PGW X-ray 4.40 G/J 1-76 [» ]
    3S6N X-ray 2.50 G 1-76 [» ]
    4F77 X-ray 3.10 4/H/P/X/f/n/v 1-76 [» ]
    4F7U X-ray 1.90 G/J 1-76 [» ]
    ProteinModelPortali P62308.
    SMRi P62308. Positions 7-74.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112521. 40 interactions.
    DIPi DIP-34627N.
    IntActi P62308. 23 interactions.
    MINTi MINT-1527983.
    STRINGi 9606.ENSP00000272348.

    PTM databases

    PhosphoSitei P62308.

    Polymorphism databases

    DMDMi 59800216.

    Proteomic databases

    MaxQBi P62308.
    PeptideAtlasi P62308.
    PRIDEi P62308.

    Protocols and materials databases

    DNASUi 6637.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000272348 ; ENSP00000272348 ; ENSG00000143977 .
    GeneIDi 6637.
    KEGGi hsa:6637.
    UCSCi uc002sgp.3. human.

    Organism-specific databases

    CTDi 6637.
    GeneCardsi GC02M070508.
    HGNCi HGNC:11163. SNRPG.
    MIMi 603542. gene.
    neXtProti NX_P62308.
    PharmGKBi PA36004.
    GenAtlasi Search...

    Phylogenomic databases

    HOVERGENi HBG000513.
    InParanoidi P62308.
    KOi K11099.
    OMAi RSAYVCR.
    OrthoDBi EOG7S221K.
    TreeFami TF315099.

    Enzyme and pathway databases

    Reactomei REACT_11066. snRNP Assembly.
    REACT_1364. SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_185. SLBP independent Processing of Histone Pre-mRNAs.
    REACT_467. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    ChiTaRSi SNRPG. human.
    EvolutionaryTracei P62308.
    GeneWikii SNRPG.
    GenomeRNAii 6637.
    NextBioi 25857.
    PROi P62308.
    SOURCEi Search...

    Gene expression databases

    Bgeei P62308.
    CleanExi HS_SNRPG.
    Genevestigatori P62308.

    Family and domain databases

    InterProi IPR010920. LSM_dom.
    IPR001163. Ribonucl_LSM.
    IPR006649. Ribonucl_LSM_euk/arc.
    [Graphical view ]
    Pfami PF01423. LSM. 1 hit.
    [Graphical view ]
    SMARTi SM00651. Sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50182. SSF50182. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions."
      Hermann H., Fabrizio P., Raker V.A., Foulaki K., Hornig H., Brahms H., Luehrmann R.
      EMBO J. 14:2076-2088(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone, Brain, Pancreas and Placenta.
    6. "Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein."
      Pillai R.S., Will C.L., Luehrmann R., Schuemperli D., Mueller B.
      EMBO J. 20:5470-5479(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE U7 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Carcinogenesis and translational controls: TACC1 is down-regulated in human cancers and associates with mRNA regulators."
      Conte N., Charafe-Jauffret E., Delaval B., Adelaide J., Ginestier C., Geneix J., Isnardon D., Jacquemier J., Birnbaum D.
      Oncogene 21:5619-5630(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TACC1.
    8. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    9. "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
      Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R.
      RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs."
      Chari A., Golas M.M., Klingenhager M., Neuenkirchen N., Sander B., Englbrecht C., Sickmann A., Stark H., Fischer U.
      Cell 135:497-509(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SNRNP BIOGENESIS, IDENTIFICATION IN 6S PICLN-SM COMPLEX, IDENTIFICATION IN SMN-SM COMPLEX, SUBCELLULAR LOCATION.
    11. "Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution."
      Pomeranz Krummel D.A., Oubridge C., Leung A.K., Li J., Nagai K.
      Nature 458:475-480(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (5.49 ANGSTROMS) OF 1-174 IN SPLICEOSOMAL U1 SNRNP.
    12. "Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis."
      Leung A.K., Nagai K., Li J.
      Nature 473:536-539(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.60 ANGSTROMS) IN SPLICEOSOMAL CORE U4 SNRNP.
    13. "Structural basis of assembly chaperone-mediated snRNP formation."
      Grimm C., Chari A., Pelz J.P., Kuper J., Kisker C., Diederichs K., Stark H., Schindelin H., Fischer U.
      Mol. Cell 49:692-703(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN 6S PICLN-SM COMPLEX, IDENTIFICATION IN 6S PICLN-SM COMPLEX, FUNCTION IN CORE U1 SNRNP BIOGENESIS.

    Entry informationi

    Entry nameiRUXG_HUMAN
    AccessioniPrimary (citable) accession number: P62308
    Secondary accession number(s): D6W5G6, Q15357, Q6IB86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3