Reviewed, UniProtKB/Swiss-Prot P62306 (RUXF_HUMAN)

Names and origin

Protein names

Recommended name:
    Small nuclear ribonucleoprotein F
      Short name=snRNP-F
      Short name=Sm protein F
      Short name=Sm-F
      Short name=SmF

Gene names

Name:	SNRPF
Synonyms:	PBSCF

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	86 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
Subunit structure	Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Formation of the U7 snRNP is an ATP-dependent process mediated by a specialized SMN complex containing at least the Sm protein core complex and additionally, the U7-specific LSM10 and LSM11 proteins. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	spliceosomal snRNP assembly Inferred from Experiment. Source: Reactome
Cellular component	U7 snRNP Ref.7 Inferred from direct assay. Source: UniProtKB cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome spliceosomal complex Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	RNA binding Ref.1 Inferred from mutant phenotype. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD2BP2	O95400	1	EBI-356900,EBI-768015
LSM5	Q9Y4Y9	1	EBI-356900,EBI-373007
OGG1	O15527	1	EBI-356900,EBI-721675
UQCRC1	P31930	1	EBI-356900,EBI-1052596

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 86	85	Small nuclear ribonucleoprotein F		PRO_0000125536
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.6
Experimental info
	Sequence conflict	86	1	E → D in CAG33032. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P62306-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 416B88D575DAE58A	FASTA	86	9,725
	10 20 30 40 50 60 MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG 70 80 EVLIRCNNVL YIRGVEEEEE DGEMRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions." Hermann H., Fabrizio P., Raker V.A., Foulaki K., Hornig H., Brahms H., Luehrmann R. EMBO J. 14:2076-2088(1995) [PubMed: 7744013] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix carcinoma.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[6]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-22 AND 66-86, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[7]	"Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein." Pillai R.S., Will C.L., Luehrmann R., Schuemperli D., Mueller B. EMBO J. 20:5470-5479(2001) [PubMed: 11574479] [Abstract] Cited for: IDENTIFICATION IN THE U7 SNRNP COMPLEX.
[8]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X85372 mRNA. Translation: CAA59688.1.
CR456751 mRNA. Translation: CAG33032.1.
AK311752 mRNA. Translation: BAG34695.1.
CH471054 Genomic DNA. Translation: EAW97548.1.
BC002505 mRNA. Translation: AAH02505.3.
BC063397 mRNA. Translation: AAH63397.2.
BC128452 mRNA. Translation: AAI28453.1.
BC128453 mRNA. Translation: AAI28454.1.

IPI

IPI00220528.

PIR

S55053.

RefSeq

NP_003086.1.

UniGene

Hs.105465

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CW1	X-ray	5.49	1/2/F/Z	1-86	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P62306. 12 interactions.

STRING

P62306.

Proteomic databases

PeptideAtlas

P62306.

PRIDE

P62306.

Genome annotation databases

Ensembl

ENST00000266735; ENSP00000266735; ENSG00000139343; Homo sapiens. [Genome view]

GeneID

6636.

KEGG

hsa:6636.

UCSC

uc001tej.1. human.

Organism-specific databases

CTD

6636.

GeneCards

GC12P094755.

H-InvDB

HIX0036777.

HGNC

HGNC:11162. SNRPF.

MIM

603541. gene.

PharmGKB

PA36003.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21099.

HOVERGEN

P62306.

InParanoid

P62306.

OMA

PKPFLNS.

PhylomeDB

P62306.

Enzyme and pathway databases

Reactome

REACT_11052. Metabolism of non-coding RNA.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_6167. Influenza Infection.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P62306.

Bgee

P62306.

CleanEx

HS_SNRPF.

Genevestigator

P62306.

GermOnline

ENSG00000139343. Homo sapiens.

Family and domain databases

InterPro

IPR001163. LSM_domain.
IPR006649. LSM_domain_euk/arc.
IPR010920. LSM_related_domain.
IPR016487. snRNP_SmF.
[Graphical view]

Pfam

PF01423. LSM. 1 hit.
[Graphical view]

PIRSF

PIRSF006609. snRNP_SmF. 1 hit.

SMART

SM00651. Sm. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

25853.

PMAP-CutDB

P62306.

SOURCE

Search...

Entry information

Entry name

RUXF_HUMAN

Accession

Primary (citable) accession number: P62306
Secondary accession number(s): A2VCR2 , B2R498, Q15356, Q6IBQ1, Q6P4I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.