ID   RUXE_MOUSE              Reviewed;          92 AA.
AC   P62305; P08578; Q15498;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 143.
DE   RecName: Full=Small nuclear ribonucleoprotein E;
DE            Short=snRNP-E;
DE   AltName: Full=Sm protein E;
DE            Short=Sm-E;
DE            Short=SmE;
GN   Name=Snrpe;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=1478669; DOI=10.1016/s0888-7543(05)80109-0;
RA   Fautsch M., Thompson M.A., Holicky E.L., Schultz P.J., Hallett J.B.,
RA   Wieben E.D.;
RT   "Conservation of coding and transcriptional control sequences within the
RT   snRNP E protein gene.";
RL   Genomics 14:883-890(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Placenta;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas,
RC   Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Plays a role in pre-mRNA splicing as a core component of the
CC       spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins
CC       (snRNPs), the building blocks of the spliceosome. Component of both the
CC       pre-catalytic spliceosome B complex and activated spliceosome C
CC       complexes. As a component of the minor spliceosome, involved in the
CC       splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it
CC       is involved in histone 3'-end processing.
CC       {ECO:0000250|UniProtKB:P62304}.
CC   -!- SUBUNIT: Core component of the spliceosomal U1, U2, U4 and U5 small
CC       nuclear ribonucleoproteins (snRNPs), the building blocks of the
CC       spliceosome. Most spliceosomal snRNPs contain a common set of Sm
CC       proteins, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that
CC       assemble in a heptameric protein ring on the Sm site of the small
CC       nuclear RNA to form the core snRNP. Component of the U1 snRNP. The U1
CC       snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB,
CC       SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG, and at least three U1
CC       snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C.
CC       Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and
CC       U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200,
CC       TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG,
CC       DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3,
CC       LSM4, LSM5, LSM6, LSM7 and LSM8. Component of the U7 snRNP complex, or
CC       U7 Sm protein core complex, that is composed of the U7 snRNA and at
CC       least LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF and SNRPG; the complex
CC       does not contain SNRPD1 and SNRPD2. Component of the minor spliceosome,
CC       which splices U12-type introns. Part of the SMN-Sm complex that
CC       contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6,
CC       GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2,
CC       SNRPD3, SNRPE, SNRPF and SNRPG; catalyzes core snRNPs assembly. Forms a
CC       6S pICln-Sm complex composed of CLNS1A/pICln, SNRPD1, SNRPD2, SNRPE,
CC       SNRPF and SNRPG; ring-like structure where CLNS1A/pICln mimics
CC       additional Sm proteins and which is unable to assemble into the core
CC       snRNP. Interacts with SMN1; the interaction is direct. Interacts with
CC       GEMIN2 (via N-terminus); the interaction is direct. Interacts with
CC       SNRPF; the interaction is direct. Interacts with SNRPG; the interaction
CC       is direct. {ECO:0000250|UniProtKB:P62304}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P62304}. Nucleus {ECO:0000250|UniProtKB:P62304}.
CC       Note=SMN-mediated assembly into core snRNPs occurs in the cytosol
CC       before SMN-mediated transport to the nucleus to be included in
CC       spliceosomes. {ECO:0000250|UniProtKB:P62304}.
CC   -!- SIMILARITY: Belongs to the snRNP Sm proteins family. {ECO:0000305}.
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DR   EMBL; X65703; CAA46625.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X65704; CAA46626.1; -; mRNA.
DR   EMBL; AK019462; BAB31734.1; -; mRNA.
DR   EMBL; BC008262; AAH08262.1; -; mRNA.
DR   EMBL; BC051207; AAH51207.1; -; mRNA.
DR   EMBL; BC055765; AAH55765.1; -; mRNA.
DR   CCDS; CCDS48364.1; -.
DR   PIR; I48778; I48778.
DR   RefSeq; NP_033253.1; NM_009227.3.
DR   AlphaFoldDB; P62305; -.
DR   SMR; P62305; -.
DR   BioGRID; 203380; 32.
DR   IntAct; P62305; 4.
DR   MINT; P62305; -.
DR   STRING; 10090.ENSMUSP00000128400; -.
DR   iPTMnet; P62305; -.
DR   PhosphoSitePlus; P62305; -.
DR   SwissPalm; P62305; -.
DR   EPD; P62305; -.
DR   jPOST; P62305; -.
DR   MaxQB; P62305; -.
DR   PaxDb; 10090-ENSMUSP00000128400; -.
DR   PeptideAtlas; P62305; -.
DR   ProteomicsDB; 256664; -.
DR   Pumba; P62305; -.
DR   TopDownProteomics; P62305; -.
DR   Antibodypedia; 53843; 230 antibodies from 24 providers.
DR   DNASU; 20643; -.
DR   Ensembl; ENSMUST00000166915.8; ENSMUSP00000128400.2; ENSMUSG00000090553.9.
DR   GeneID; 20643; -.
DR   KEGG; mmu:20643; -.
DR   UCSC; uc007cql.2; mouse.
DR   AGR; MGI:98346; -.
DR   CTD; 6635; -.
DR   MGI; MGI:98346; Snrpe.
DR   VEuPathDB; HostDB:ENSMUSG00000090553; -.
DR   eggNOG; KOG1774; Eukaryota.
DR   GeneTree; ENSGT00390000012818; -.
DR   HOGENOM; CLU_125186_1_0_1; -.
DR   InParanoid; P62305; -.
DR   OMA; VPPINCI; -.
DR   OrthoDB; 5113494at2759; -.
DR   PhylomeDB; P62305; -.
DR   TreeFam; TF314419; -.
DR   Reactome; R-MMU-111367; SLBP independent Processing of Histone Pre-mRNAs.
DR   Reactome; R-MMU-191859; snRNP Assembly.
DR   Reactome; R-MMU-72163; mRNA Splicing - Major Pathway.
DR   Reactome; R-MMU-72165; mRNA Splicing - Minor Pathway.
DR   Reactome; R-MMU-73856; RNA Polymerase II Transcription Termination.
DR   Reactome; R-MMU-77588; SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs.
DR   BioGRID-ORCS; 20643; 38 hits in 116 CRISPR screens.
DR   ChiTaRS; Snrpe; mouse.
DR   PRO; PR:P62305; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; P62305; Protein.
DR   Bgee; ENSMUSG00000090553; Expressed in medial ganglionic eminence and 255 other cell types or tissues.
DR   ExpressionAtlas; P62305; baseline and differential.
DR   GO; GO:0071013; C:catalytic step 2 spliceosome; ISO:MGI.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0034709; C:methylosome; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0034715; C:pICln-Sm protein complex; ISS:UniProtKB.
DR   GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR   GO; GO:0034719; C:SMN-Sm protein complex; ISS:UniProtKB.
DR   GO; GO:0005681; C:spliceosomal complex; ISO:MGI.
DR   GO; GO:0005697; C:telomerase holoenzyme complex; ISO:MGI.
DR   GO; GO:0005685; C:U1 snRNP; ISS:UniProtKB.
DR   GO; GO:0005689; C:U12-type spliceosomal complex; ISO:MGI.
DR   GO; GO:0005686; C:U2 snRNP; IBA:GO_Central.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; ISS:UniProtKB.
DR   GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; ISS:UniProtKB.
DR   GO; GO:0005687; C:U4 snRNP; ISS:UniProtKB.
DR   GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; ISS:UniProtKB.
DR   GO; GO:0005682; C:U5 snRNP; IBA:GO_Central.
DR   GO; GO:0005683; C:U7 snRNP; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:1990446; F:U1 snRNP binding; ISO:MGI.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR   GO; GO:0000387; P:spliceosomal snRNP assembly; ISS:UniProtKB.
DR   CDD; cd01718; Sm_E; 1.
DR   Gene3D; 2.30.30.100; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR047575; Sm.
DR   InterPro; IPR001163; Sm_dom_euk/arc.
DR   InterPro; IPR027078; snRNP-E.
DR   PANTHER; PTHR11193; SMALL NUCLEAR RIBONUCLEOPROTEIN E; 1.
DR   PANTHER; PTHR11193:SF9; SMALL NUCLEAR RIBONUCLEOPROTEIN E; 1.
DR   Pfam; PF01423; LSM; 1.
DR   SMART; SM00651; Sm; 1.
DR   SUPFAM; SSF50182; Sm-like ribonucleoproteins; 1.
DR   PROSITE; PS52002; SM; 1.
DR   Genevisible; P62305; MM.
PE   1: Evidence at protein level;
KW   Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Spliceosome.
FT   CHAIN           1..92
FT                   /note="Small nuclear ribonucleoprotein E"
FT                   /id="PRO_0000125530"
FT   DOMAIN          18..92
FT                   /note="Sm"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01346"
SQ   SEQUENCE   92 AA;  10804 MW;  7D8881CE1F4FA2FD CRC64;
     MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD
     AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN
//