Reviewed, UniProtKB/Swiss-Prot P62304 (RUXE_HUMAN)

Names and origin

Protein names

Recommended name:
    Small nuclear ribonucleoprotein E
      Short name=snRNP-E
      Short name=Sm protein E
      Short name=Sm-E
      Short name=SmE

Gene names

Name:

SNRPE

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
Subunit structure	Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Formation of the U7 snRNP is an ATP-dependent process mediated by a specialized SMN complex containing at least the Sm protein core complex and additionally, the U7-specific LSM10 and LSM11 proteins. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
Subcellular location	Nucleus.
Involvement in disease	Patients with systemic lupus erythematosus produces antibodies which interact with snRNP proteins.
Sequence similarities	Belongs to the snRNP Sm proteins family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Disease	Systemic lupus erythematosus
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	spliceosomal snRNP assembly Inferred from Experiment. Source: Reactome spliceosome assembly Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	U7 snRNP Ref.7 Inferred from direct assay. Source: UniProtKB cytosol Inferred from Experiment. Source: Reactome nucleoplasm Inferred from Experiment. Source: Reactome spliceosomal complex Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GEMIN5	Q8TEQ6	1	EBI-348082,EBI-443630
LSM2	Q9Y333	2	EBI-348082,EBI-347416
LSM3	P62310	1	EBI-348082,EBI-348239
LSM4	Q9Y4Z0	1	EBI-348082,EBI-372521
LSM5	Q9Y4Y9	1	EBI-348082,EBI-373007

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 92	92	Small nuclear ribonucleoprotein E		PRO_0000125529

Sequences

	Sequence		Length	Mass (Da)
	P62304-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7D8881CE1F4FA2FD	FASTA	92	10,804
	10 20 30 40 50 60 MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD 70 80 90 AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete primary structure of the human snRNP E protein." Stanford D.R., Kehl M., Perry C.A., Holicky E., Harvey S.E., Rohleder A.M., Rehder K., Luehrmann R., Wieben E.D. Nucleic Acids Res. 16:10593-10605(1988) [PubMed: 2974536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The small nuclear ribonucleoprotein E protein gene contains four introns and has upstream similarities to genes for ribosomal proteins." Stanford D.R., Perry C.A., Holicky E., Rohleder A.M., Wieben E.D. J. Biol. Chem. 263:17772-17779(1988) [PubMed: 2972723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Uterus.
[6]	"DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene." Stanford D.R., Rohleder A., Neiswanger K., Wieben E.D. J. Biol. Chem. 262:9931-9934(1987) [PubMed: 2440864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-92.
[7]	"Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein." Pillai R.S., Will C.L., Luehrmann R., Schuemperli D., Mueller B. EMBO J. 20:5470-5479(2001) [PubMed: 11574479] [Abstract] Cited for: IDENTIFICATION IN THE U7 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M37716 mRNA. Translation: AAA90926.1.
M15919 mRNA. Translation: AAA36621.1.
X12466 mRNA. Translation: CAA31007.1.
AK312130 mRNA. Translation: BAG35066.1.
CH471067 Genomic DNA. Translation: EAW91494.1.
BC002639 mRNA. Translation: AAH02639.1.
BC090951 mRNA. Translation: AAH90951.1.
M21258, M21253 Genomic DNA. Translation: AAB59365.1. Sequence problems.

IPI

IPI00029266.

PIR

A32127.

RefSeq

NP_003085.1.

UniGene

Hs.334612
Hs.654418

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CW1	X-ray	5.49	E/W/X/Y	1-92	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P62304. 13 interactions.

STRING

P62304.

Proteomic databases

PRIDE

P62304.

Genome annotation databases

Ensembl

ENST00000414487; ENSP00000400591; ENSG00000182004; Homo sapiens. [Genome view]

GeneID

6635.

KEGG

hsa:6635.

UCSC

uc001hai.1. human.

Organism-specific databases

CTD

6635.

GeneCards

GC01P202097.
GC19M005531.

HGNC

HGNC:11161. SNRPE.

MIM

128260. gene.

PharmGKB

PA36002.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21276.

HOGENOM

HBG749684.

HOVERGEN

P62304.

InParanoid

P62304.

OMA

EGHIVGF.

PhylomeDB

P62304.

Enzyme and pathway databases

Reactome

REACT_11052. Metabolism of non-coding RNA.
REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_6167. Influenza Infection.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P62304.

Bgee

P62304.

CleanEx

HS_SNRPE.

Genevestigator

P62304.

GermOnline

ENSG00000182004. Homo sapiens.

Family and domain databases

InterPro

IPR001163. LSM_domain.
IPR006649. LSM_domain_euk/arc.
IPR010920. LSM_related_domain.
[Graphical view]

Pfam

PF01423. LSM. 1 hit.
[Graphical view]

SMART

SM00651. Sm. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

25849.

SOURCE

Search...

Entry information

Entry name

RUXE_HUMAN

Accession

Primary (citable) accession number: P62304
Secondary accession number(s): B2R5B9 , P08578, Q15498, Q5BKT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.