P62304 (RUXE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small nuclear ribonucleoprotein E
Short name=snRNP-E

Alternative name(s):
Sm protein E
Short name=Sm-E
Short name=SmE

Gene names

Name:

SNRPE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
Subunit structure	Component of the heptameric ring U7 snRNP complex, or U7 Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Formation of the U7 snRNP is an ATP-dependent process mediated by a specialized SMN complex containing at least the Sm protein core complex and additionally, the U7-specific LSM10 and LSM11 proteins. Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus.
Miscellaneous	Patients with systemic lupus erythematosus produce antibodies which interact with snRNP proteins.
Sequence similarities	Belongs to the snRNP Sm proteins family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone mRNA metabolic process Traceable author statement. Source: Reactome ncRNA metabolic process Traceable author statement. Source: Reactome spliceosomal complex assembly Non-traceable author statement Ref.1. Source: UniProtKB spliceosomal snRNP assembly Traceable author statement. Source: Reactome termination of RNA polymerase II transcription Traceable author statement. Source: Reactome
Cellular_component	U12-type spliceosomal complex Inferred from direct assay Ref.9. Source: UniProtKB U7 snRNP Inferred from direct assay Ref.7. Source: UniProtKB catalytic step 2 spliceosome Inferred from direct assay Ref.8. Source: UniProtKB cytosol Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GEMIN5	Q8TEQ6	2	EBI-348082,EBI-443630

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 92

92

Small nuclear ribonucleoprotein E

PRO_0000125529

Secondary structure

1

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92

Helix Strand Turn

Details...

Helix

17 – 27

11

Beta strand

30 – 38

9

Beta strand

40 – 50

11

Beta strand

56 – 64

9

Beta strand

66 – 68

3

Beta strand

71 – 79

9

Helix

81 – 83

3

Beta strand

84 – 89

6

Sequences

	Sequence		Length	Mass (Da)
	P62304 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7D8881CE1F4FA2FD	FASTA	92	10,804
	10 20 30 40 50 60 MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD 70 80 90 AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete primary structure of the human snRNP E protein." Stanford D.R., Kehl M., Perry C.A., Holicky E., Harvey S.E., Rohleder A.M., Rehder K., Luehrmann R., Wieben E.D. Nucleic Acids Res. 16:10593-10605(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The small nuclear ribonucleoprotein E protein gene contains four introns and has upstream similarities to genes for ribosomal proteins." Stanford D.R., Perry C.A., Holicky E., Rohleder A.M., Wieben E.D. J. Biol. Chem. 263:17772-17779(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cerebellum.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Uterus.
[6]	"DNA sequence of a human Sm autoimmune antigen. The multigene family contains a processed pseudogene." Stanford D.R., Rohleder A., Neiswanger K., Wieben E.D. J. Biol. Chem. 262:9931-9934(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-92.
[7]	"Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein." Pillai R.S., Will C.L., Luehrmann R., Schuemperli D., Mueller B. EMBO J. 20:5470-5479(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE U7 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[9]	"The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome." Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R. RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH THE U11/U12 SPLICEOSOME, MASS SPECTROMETRY.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M37716 mRNA. Translation: AAA90926.1.
M15919 mRNA. Translation: AAA36621.1.
X12466 mRNA. Translation: CAA31007.1.
AK312130 mRNA. Translation: BAG35066.1.
CH471067 Genomic DNA. Translation: EAW91494.1.
BC002639 mRNA. Translation: AAH02639.1.
BC090951 mRNA. Translation: AAH90951.1.
M21258, M21253 Genomic DNA. Translation: AAB59365.1. Sequence problems.

IPI

IPI00029266.

PIR

A32127.

RefSeq

NP_003085.1. NM_003094.2.

UniGene

Hs.334612.
Hs.654418.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VU2	X-ray	3.10	C/K/S/a/i/q/y	1-92	[»]
1VU3	X-ray	3.10	C/K/S/a/i/q	1-92	[»]
2Y9A	X-ray	3.60	E/L/S	1-92	[»]
2Y9B	X-ray	3.60	E/L/S	1-92	[»]
2Y9C	X-ray	3.60	E/L/S	1-92	[»]
2Y9D	X-ray	3.60	E/L/S	1-92	[»]
3CW1	X-ray	5.49	E/W/X/Y	1-92	[»]
3PGW	X-ray	4.40	E/H	1-92	[»]
3S6N	X-ray	2.50	E	1-92	[»]
4F77	X-ray	3.10	C/K/S/a/i/q/y	1-92	[»]

ProteinModelPortal

P62304.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31220N.

IntAct

P62304. 26 interactions.

MINT

MINT-225773.

STRING

9606.ENSP00000400591.

PTM databases

PhosphoSite

P62304.

Polymorphism databases

DMDM

61237380.

Proteomic databases

PaxDb

P62304.

PRIDE

P62304.

Protocols and materials databases

DNASU

6635.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000414487; ENSP00000400591; ENSG00000182004.

GeneID

6635.

KEGG

hsa:6635.

UCSC

uc001hai.3. human.

Organism-specific databases

CTD

6635.

GeneCards

GC01P203834.

HGNC

HGNC:11161. SNRPE.

MIM

128260. gene.

neXtProt

NX_P62304.

PharmGKB

PA36002.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1958.

HOVERGEN

HBG003229.

InParanoid

P62304.

KO

K11097.

OMA

VQIWLYE.

OrthoDB

EOG4DBTGC.

Enzyme and pathway databases

Reactome

REACT_1675. mRNA Processing.
REACT_1788. Transcription.
REACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.
REACT_78. Post-Elongation Processing of the Transcript.

Gene expression databases

ArrayExpress

P62304.

Bgee

P62304.

CleanEx

HS_SNRPE.

Genevestigator

P62304.

GermOnline

ENSG00000182004. Homo sapiens.

Family and domain databases

InterPro

IPR010920. LSM_dom.
IPR001163. Ribonucl_LSM.
IPR006649. Ribonucl_LSM_euk/arc.
IPR027078. snRNP-E.
[Graphical view]

PANTHER

PTHR11193. PTHR11193. 1 hit.

Pfam

PF01423. LSM. 1 hit.
[Graphical view]

SMART

SM00651. Sm. 1 hit.
[Graphical view]

SUPFAM

SSF50182. Sm_like_riboprot. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

SNRPE. human.

EvolutionaryTrace

P62304.

GenomeRNAi

6635.

NextBio

25849.

SOURCE

Search...

Entry information

Entry name

RUXE_HUMAN

Accession

Primary (citable) accession number: P62304
Secondary accession number(s): B2R5B9 , P08578, Q15498, Q5BKT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.