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Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • poly(A) RNA binding Source: Ensembl
  • potassium channel regulator activity Source: Ensembl
  • protein complex binding Source: RGD
  • protein kinase C inhibitor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-2028269. Signaling by Hippo.
R-RNO-205025. NADE modulates death signalling.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-3371453. Regulation of HSF1-mediated heat shock response.
R-RNO-3371511. HSF1 activation.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8854518. AURKA Activation by TPX2.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Alternative name(s):
Mitochondrial import stimulation factor L subunit
Short name:
MSF L
Gene namesi
Name:Ywhae
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi62000. Ywhae.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cell-cell adherens junction Source: Ensembl
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • kinesin complex Source: RGD
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: Ensembl
  • mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586201 – 25514-3-3 protein epsilonAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei65PhosphoserineCombined sources1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei123N6-acetyllysineBy similarity1
Modified residuei131PhosphotyrosineCombined sources1
Modified residuei137PhosphothreonineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei232PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62260.
PRIDEiP62260.

2D gel databases

World-2DPAGE0004:P62260.

PTM databases

iPTMnetiP62260.
PhosphoSitePlusiP62260.

Expressioni

Developmental stagei

Present at high levels in the pineal gland early in development and decreased steadily thereafter.1 Publication

Gene expression databases

BgeeiENSRNOG00000005290.
GenevisibleiP62260. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimerizes with YWHAZ (By similarity). Interacts with PKA-phosphorylated AANAT (PubMed:11427721). Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with ARHGEF28 (By similarity). Interacts with BEX3 (By similarity). Weakly interacts with CDKN1B (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with DENND1A (By similarity). Interacts with GAB2 (By similarity). Interacts with phosphorylated GRB10 (By similarity). Interacts with KSR1 (By similarity). Interacts with NDEL1 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with the phosphorylated (by AKT1) form of SRPK2 (By similarity). Interacts with TIAM2 (By similarity). Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (PubMed:22827337). Interacts with ZFP36 (via phosphorylated form) (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei130Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
CSNK1A1P678282EBI-356462,EBI-7540603From a different organism.
FBXO4Q9UKT52EBI-356462,EBI-960409From a different organism.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi248364. 8 interactors.
DIPiDIP-37260N.
IntActiP62260. 10 interactors.
MINTiMINT-1597410.
STRINGi10116.ENSRNOP00000007100.

Structurei

3D structure databases

ProteinModelPortaliP62260.
SMRiP62260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP62260.
KOiK06630.
OMAiKESALIM.
OrthoDBiEOG091G0VKY.
PhylomeDBiP62260.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
110 120 130 140 150
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
160 170 180 190 200
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV

EDENQ
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73K → T in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti120F → S in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti123K → Y in AAC52676 (PubMed:8694795).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84416 mRNA. Translation: AAC37659.1.
D30739 mRNA. Translation: BAA06401.1.
U53882 mRNA. Translation: AAC52676.1.
BC063163 mRNA. Translation: AAH63163.1.
PIRiJX0341.
RefSeqiNP_113791.1. NM_031603.1.
UniGeneiRn.4225.

Genome annotation databases

EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290.
GeneIDi29753.
KEGGirno:29753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84416 mRNA. Translation: AAC37659.1.
D30739 mRNA. Translation: BAA06401.1.
U53882 mRNA. Translation: AAC52676.1.
BC063163 mRNA. Translation: AAH63163.1.
PIRiJX0341.
RefSeqiNP_113791.1. NM_031603.1.
UniGeneiRn.4225.

3D structure databases

ProteinModelPortaliP62260.
SMRiP62260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248364. 8 interactors.
DIPiDIP-37260N.
IntActiP62260. 10 interactors.
MINTiMINT-1597410.
STRINGi10116.ENSRNOP00000007100.

PTM databases

iPTMnetiP62260.
PhosphoSitePlusiP62260.

2D gel databases

World-2DPAGE0004:P62260.

Proteomic databases

PaxDbiP62260.
PRIDEiP62260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290.
GeneIDi29753.
KEGGirno:29753.

Organism-specific databases

CTDi7531.
RGDi62000. Ywhae.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP62260.
KOiK06630.
OMAiKESALIM.
OrthoDBiEOG091G0VKY.
PhylomeDBiP62260.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-2028269. Signaling by Hippo.
R-RNO-205025. NADE modulates death signalling.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-3371453. Regulation of HSF1-mediated heat shock response.
R-RNO-3371511. HSF1 activation.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8854518. AURKA Activation by TPX2.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

PROiP62260.

Gene expression databases

BgeeiENSRNOG00000005290.
GenevisibleiP62260. RN.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433E_RAT
AccessioniPrimary (citable) accession number: P62260
Secondary accession number(s): P29360, P42655, Q63631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.