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Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-2028269. Signaling by Hippo.
R-RNO-205025. NADE modulates death signalling.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-3371453. Regulation of HSF1-mediated heat shock response.
R-RNO-3371511. HSF1 activation.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-380320. Recruitment of NuMA to mitotic centrosomes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8854518. AURKA Activation by TPX2.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Alternative name(s):
Mitochondrial import stimulation factor L subunit
Short name:
MSF L
Gene namesi
Name:Ywhae
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi62000. Ywhae.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586201 – 25514-3-3 protein epsilonAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei50N6-acetyllysine; alternateBy similarity1
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei65PhosphoserineCombined sources1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei123N6-acetyllysineBy similarity1
Modified residuei131PhosphotyrosineCombined sources1
Modified residuei137PhosphothreonineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei232PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62260.
PRIDEiP62260.

2D gel databases

World-2DPAGEi0004:P62260.

PTM databases

iPTMnetiP62260.
PhosphoSitePlusiP62260.

Expressioni

Developmental stagei

Present at high levels in the pineal gland early in development and decreased steadily thereafter.1 Publication

Gene expression databases

BgeeiENSRNOG00000005290.
GenevisibleiP62260. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimerizes with YWHAZ (By similarity). Interacts with PKA-phosphorylated AANAT (PubMed:11427721). Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with ARHGEF28 (By similarity). Interacts with BEX3 (By similarity). Weakly interacts with CDKN1B (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with DENND1A (By similarity). Interacts with GAB2 (By similarity). Interacts with phosphorylated GRB10 (By similarity). Interacts with KSR1 (By similarity). Interacts with NDEL1 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with the phosphorylated (by AKT1) form of SRPK2 (By similarity). Interacts with TIAM2 (By similarity). Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (PubMed:22827337). Interacts with ZFP36 (via phosphorylated form) (By similarity). Interacts with SLITRK1 (By similarity). Interacts with HSF1 (via phosphorylated form); this interaction promotes HSF1 sequestration in the cytoplasm in a ERK-dependent manner (By similarity). Interacts with RIPOR2 (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei130Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248364. 11 interactors.
CORUMiP62260.
DIPiDIP-37260N.
IntActiP62260. 11 interactors.
MINTiMINT-1597410.
STRINGi10116.ENSRNOP00000007100.

Structurei

3D structure databases

ProteinModelPortaliP62260.
SMRiP62260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP62260.
KOiK06630.
OMAiIPCATTG.
OrthoDBiEOG091G0VKY.
PhylomeDBiP62260.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR036815. 14-3-3_dom_sf.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P62260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
110 120 130 140 150
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
160 170 180 190 200
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV

EDENQ
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73K → T in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti120F → S in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti123K → Y in AAC52676 (PubMed:8694795).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84416 mRNA. Translation: AAC37659.1.
D30739 mRNA. Translation: BAA06401.1.
U53882 mRNA. Translation: AAC52676.1.
BC063163 mRNA. Translation: AAH63163.1.
PIRiJX0341.
RefSeqiNP_113791.1. NM_031603.1.
UniGeneiRn.4225.

Genome annotation databases

EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290.
GeneIDi29753.
KEGGirno:29753.

Similar proteinsi

Entry informationi

Entry namei1433E_RAT
AccessioniPrimary (citable) accession number: P62260
Secondary accession number(s): P29360, P42655, Q63631
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families