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P62260

- 1433E_RAT

UniProt

P62260 - 1433E_RAT

Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei57 – 571Interaction with phosphoserine on interacting proteinBy similarity
    Sitei130 – 1301Interaction with phosphoserine on interacting proteinBy similarity

    GO - Molecular functioni

    1. enzyme binding Source: RGD
    2. potassium channel regulator activity Source: Ensembl
    3. protein binding Source: IntAct
    4. protein complex binding Source: RGD
    5. protein kinase C inhibitor activity Source: RGD

    GO - Biological processi

    1. cerebral cortex development Source: Ensembl
    2. hippocampus development Source: Ensembl
    3. negative regulation of peptidyl-serine dephosphorylation Source: Ensembl
    4. negative regulation of protein serine/threonine kinase activity Source: GOC
    5. neuron migration Source: Ensembl
    6. protein targeting Source: Ensembl
    7. regulation of membrane repolarization Source: Ensembl
    8. regulation of potassium ion transmembrane transporter activity Source: Ensembl
    9. signal transduction Source: RGD

    Enzyme and pathway databases

    ReactomeiREACT_198294. Regulation of PLK1 Activity at G2/M Transition.
    REACT_198809. Signaling by Hippo.
    REACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_205802. HSF1 activation.
    REACT_216489. Regulation of HSF1-mediated heat shock response.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    14-3-3 protein epsilon
    Short name:
    14-3-3E
    Alternative name(s):
    Mitochondrial import stimulation factor L subunit
    Short name:
    MSF L
    Gene namesi
    Name:Ywhae
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi62000. Ywhae.

    Subcellular locationi

    Cytoplasm By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. axon Source: RGD
    2. extracellular vesicular exosome Source: Ensembl
    3. kinesin complex Source: RGD
    4. melanosome Source: UniProtKB-SubCell
    5. mitochondrion Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 25525514-3-3 protein epsilonPRO_0000058620Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei69 – 691N6-acetyllysineBy similarity
    Modified residuei118 – 1181N6-acetyllysineBy similarity
    Modified residuei123 – 1231N6-acetyllysineBy similarity
    Modified residuei210 – 2101PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP62260.
    PRIDEiP62260.

    2D gel databases

    World-2DPAGE0004:P62260.

    PTM databases

    PhosphoSiteiP62260.

    Expressioni

    Developmental stagei

    Present at high levels in the pineal gland early in development and decreased steadily thereafter.1 Publication

    Gene expression databases

    GenevestigatoriP62260.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Heterodimerizes with YWHAZ By similarity. Interacts with NDEL1, ARHGEF28 and TIAM2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10 By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CSNK1A1P678282EBI-356462,EBI-7540603From a different organism.
    FBXO4Q9UKT52EBI-356462,EBI-960409From a different organism.

    Protein-protein interaction databases

    BioGridi248364. 8 interactions.
    DIPiDIP-37260N.
    IntActiP62260. 10 interactions.
    MINTiMINT-1597410.
    STRINGi10116.ENSRNOP00000007100.

    Structurei

    3D structure databases

    ProteinModelPortaliP62260.
    SMRiP62260. Positions 3-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 14-3-3 family.Curated

    Phylogenomic databases

    eggNOGiCOG5040.
    GeneTreeiENSGT00730000110613.
    HOGENOMiHOG000240379.
    HOVERGENiHBG050423.
    InParanoidiP62260.
    KOiK06630.
    OMAiMQESDKP.
    OrthoDBiEOG7HHWT3.
    PhylomeDBiP62260.
    TreeFamiTF102003.

    Family and domain databases

    Gene3Di1.20.190.20. 1 hit.
    InterProiIPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view]
    PANTHERiPTHR18860. PTHR18860. 1 hit.
    PfamiPF00244. 14-3-3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000868. 14-3-3. 1 hit.
    PRINTSiPR00305. 1433ZETA.
    SMARTiSM00101. 14_3_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48445. SSF48445. 1 hit.
    PROSITEiPS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62260-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK    50
    NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI 100
    LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV 150
    AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD 200
    DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV 250
    EDENQ 255
    Length:255
    Mass (Da):29,174
    Last modified:July 5, 2004 - v1
    Checksum:i07817CCBD1F75B26
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731K → T in AAC52676. (PubMed:8694795)Curated
    Sequence conflicti120 – 1201F → S in AAC52676. (PubMed:8694795)Curated
    Sequence conflicti123 – 1231K → Y in AAC52676. (PubMed:8694795)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84416 mRNA. Translation: AAC37659.1.
    D30739 mRNA. Translation: BAA06401.1.
    U53882 mRNA. Translation: AAC52676.1.
    BC063163 mRNA. Translation: AAH63163.1.
    PIRiJX0341.
    RefSeqiNP_113791.1. NM_031603.1.
    UniGeneiRn.4225.

    Genome annotation databases

    EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290.
    GeneIDi29753.
    KEGGirno:29753.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84416 mRNA. Translation: AAC37659.1 .
    D30739 mRNA. Translation: BAA06401.1 .
    U53882 mRNA. Translation: AAC52676.1 .
    BC063163 mRNA. Translation: AAH63163.1 .
    PIRi JX0341.
    RefSeqi NP_113791.1. NM_031603.1.
    UniGenei Rn.4225.

    3D structure databases

    ProteinModelPortali P62260.
    SMRi P62260. Positions 3-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248364. 8 interactions.
    DIPi DIP-37260N.
    IntActi P62260. 10 interactions.
    MINTi MINT-1597410.
    STRINGi 10116.ENSRNOP00000007100.

    PTM databases

    PhosphoSitei P62260.

    2D gel databases

    World-2DPAGE 0004:P62260.

    Proteomic databases

    PaxDbi P62260.
    PRIDEi P62260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007100 ; ENSRNOP00000007100 ; ENSRNOG00000005290 .
    GeneIDi 29753.
    KEGGi rno:29753.

    Organism-specific databases

    CTDi 7531.
    RGDi 62000. Ywhae.

    Phylogenomic databases

    eggNOGi COG5040.
    GeneTreei ENSGT00730000110613.
    HOGENOMi HOG000240379.
    HOVERGENi HBG050423.
    InParanoidi P62260.
    KOi K06630.
    OMAi MQESDKP.
    OrthoDBi EOG7HHWT3.
    PhylomeDBi P62260.
    TreeFami TF102003.

    Enzyme and pathway databases

    Reactomei REACT_198294. Regulation of PLK1 Activity at G2/M Transition.
    REACT_198809. Signaling by Hippo.
    REACT_199176. Translocation of GLUT4 to the plasma membrane.
    REACT_205802. HSF1 activation.
    REACT_216489. Regulation of HSF1-mediated heat shock response.

    Miscellaneous databases

    NextBioi 610291.
    PROi P62260.

    Gene expression databases

    Genevestigatori P62260.

    Family and domain databases

    Gene3Di 1.20.190.20. 1 hit.
    InterProi IPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view ]
    PANTHERi PTHR18860. PTHR18860. 1 hit.
    Pfami PF00244. 14-3-3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000868. 14-3-3. 1 hit.
    PRINTSi PR00305. 1433ZETA.
    SMARTi SM00101. 14_3_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48445. SSF48445. 1 hit.
    PROSITEi PS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins."
      Roseboom P.H., Weller J.L., Babila T., Aitken A., Sellers L.A., Moffet J.R., Namboodiri M.A., Klein D.C.
      DNA Cell Biol. 13:629-640(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Tissue: Pineal gland.
    2. "cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol."
      Alam R., Hachiya N., Sakaguchi M., Shun-Ichiro K., Iwanaga S., Kitajima M., Mihara K., Omura T.
      J. Biochem. 116:416-425(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Association of a 14-3-3 protein with CMP-NeuAc:GM1 alpha 2,3-sialyltransferase."
      Gao L., Gu X.B., Yu D.S., Yu R.K., Zeng G.
      Biochem. Biophys. Res. Commun. 224:103-107(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    5. Bienvenut W.V., von Kriegsheim A., Kolch W.
      Submitted (JUN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-12; 30-42; 95-106; 143-153 AND 216-225, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Fibroblast.
    6. Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 20-50; 62-73; 87-94; 107-123; 131-170 AND 197-225, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    7. "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
      Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C.
      Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 131-142 AND 154-170, INTERACTION WITH AANAT, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry namei1433E_RAT
    AccessioniPrimary (citable) accession number: P62260
    Secondary accession number(s): P29360, P42655, Q63631
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3