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Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-111447 Activation of BAD and translocation to mitochondria
R-RNO-1445148 Translocation of GLUT4 to the plasma membrane
R-RNO-2028269 Signaling by Hippo
R-RNO-205025 NADE modulates death signalling
R-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-3371453 Regulation of HSF1-mediated heat shock response
R-RNO-3371511 HSF1 activation
R-RNO-380259 Loss of Nlp from mitotic centrosomes
R-RNO-380270 Recruitment of mitotic centrosome proteins and complexes
R-RNO-380320 Recruitment of NuMA to mitotic centrosomes
R-RNO-5620912 Anchoring of the basal body to the plasma membrane
R-RNO-5625740 RHO GTPases activate PKNs
R-RNO-5628897 TP53 Regulates Metabolic Genes
R-RNO-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-RNO-8854518 AURKA Activation by TPX2
R-RNO-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Alternative name(s):
Mitochondrial import stimulation factor L subunit
Short name:
MSF L
Gene namesi
Name:Ywhae
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi62000 Ywhae

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586201 – 25514-3-3 protein epsilonAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei50N6-acetyllysine; alternateBy similarity1
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei65PhosphoserineCombined sources1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei118N6-acetyllysineBy similarity1
Modified residuei123N6-acetyllysineBy similarity1
Modified residuei131PhosphotyrosineCombined sources1
Modified residuei137PhosphothreonineCombined sources1
Modified residuei210PhosphoserineCombined sources1
Modified residuei232PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62260
PRIDEiP62260

2D gel databases

World-2DPAGE0004:P62260

PTM databases

CarbonylDBiP62260
iPTMnetiP62260
PhosphoSitePlusiP62260

Expressioni

Developmental stagei

Present at high levels in the pineal gland early in development and decreased steadily thereafter.1 Publication

Gene expression databases

BgeeiENSRNOG00000005290
GenevisibleiP62260 RN

Interactioni

Subunit structurei

Homodimer (By similarity). Heterodimerizes with YWHAZ (By similarity). Interacts with PKA-phosphorylated AANAT (PubMed:11427721). Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm (By similarity). Interacts with ARHGEF28 (By similarity). Interacts with BEX3 (By similarity). Weakly interacts with CDKN1B (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with DENND1A (By similarity). Interacts with GAB2 (By similarity). Interacts with phosphorylated GRB10 (By similarity). Interacts with KSR1 (By similarity). Interacts with NDEL1 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with the phosphorylated (by AKT1) form of SRPK2 (By similarity). Interacts with TIAM2 (By similarity). Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (PubMed:22827337). Interacts with ZFP36 (via phosphorylated form) (By similarity). Interacts with SLITRK1 (By similarity). Interacts with HSF1 (via phosphorylated form); this interaction promotes HSF1 sequestration in the cytoplasm in a ERK-dependent manner (By similarity). Interacts with RIPOR2 (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei130Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248364, 12 interactors
CORUMiP62260
DIPiDIP-37260N
IntActiP62260, 11 interactors
MINTiP62260
STRINGi10116.ENSRNOP00000007100

Structurei

3D structure databases

ProteinModelPortaliP62260
SMRiP62260
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiP62260
KOiK06630
OMAiIPCATTG
OrthoDBiEOG091G0VKY
PhylomeDBiP62260
TreeFamiTF102003

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

P62260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
110 120 130 140 150
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
160 170 180 190 200
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV

EDENQ
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73K → T in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti120F → S in AAC52676 (PubMed:8694795).Curated1
Sequence conflicti123K → Y in AAC52676 (PubMed:8694795).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84416 mRNA Translation: AAC37659.1
D30739 mRNA Translation: BAA06401.1
U53882 mRNA Translation: AAC52676.1
BC063163 mRNA Translation: AAH63163.1
PIRiJX0341
RefSeqiNP_113791.1, NM_031603.1
UniGeneiRn.4225

Genome annotation databases

EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290
GeneIDi29753
KEGGirno:29753

Similar proteinsi

Entry informationi

Entry namei1433E_RAT
AccessioniPrimary (citable) accession number: P62260
Secondary accession number(s): P29360, P42655, Q63631
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health