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P62260

- 1433E_RAT

UniProt

P62260 - 1433E_RAT

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Protein
14-3-3 protein epsilon
Gene
Ywhae
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Interaction with phosphoserine on interacting protein By similarity
Sitei130 – 1301Interaction with phosphoserine on interacting protein By similarity

GO - Molecular functioni

  1. enzyme binding Source: RGD
  2. potassium channel regulator activity Source: Ensembl
  3. protein binding Source: IntAct
  4. protein complex binding Source: RGD
  5. protein kinase C inhibitor activity Source: RGD

GO - Biological processi

  1. cerebral cortex development Source: Ensembl
  2. hippocampus development Source: Ensembl
  3. negative regulation of peptidyl-serine dephosphorylation Source: Ensembl
  4. negative regulation of protein serine/threonine kinase activity Source: GOC
  5. neuron migration Source: Ensembl
  6. protein targeting Source: Ensembl
  7. regulation of membrane repolarization Source: Ensembl
  8. regulation of potassium ion transmembrane transporter activity Source: Ensembl
  9. signal transduction Source: RGD
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_198294. Regulation of PLK1 Activity at G2/M Transition.
REACT_198809. Signaling by Hippo.
REACT_199176. Translocation of GLUT4 to the plasma membrane.
REACT_205802. HSF1 activation.
REACT_216489. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Alternative name(s):
Mitochondrial import stimulation factor L subunit
Short name:
MSF L
Gene namesi
Name:Ywhae
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi62000. Ywhae.

Subcellular locationi

Cytoplasm By similarity. Melanosome By similarity

GO - Cellular componenti

  1. axon Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
  3. kinesin complex Source: RGD
  4. melanosome Source: UniProtKB-SubCell
  5. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 25525514-3-3 protein epsilon
PRO_0000058620Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei50 – 501N6-acetyllysine By similarity
Modified residuei69 – 691N6-acetyllysine By similarity
Modified residuei118 – 1181N6-acetyllysine By similarity
Modified residuei123 – 1231N6-acetyllysine By similarity
Modified residuei210 – 2101Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62260.
PRIDEiP62260.

2D gel databases

World-2DPAGE0004:P62260.

PTM databases

PhosphoSiteiP62260.

Expressioni

Developmental stagei

Present at high levels in the pineal gland early in development and decreased steadily thereafter.1 Publication

Gene expression databases

GenevestigatoriP62260.

Interactioni

Subunit structurei

Homodimer By similarity. Heterodimerizes with YWHAZ By similarity. Interacts with NDEL1, ARHGEF28 and TIAM2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10 By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSNK1A1P678282EBI-356462,EBI-7540603From a different organism.
FBXO4Q9UKT52EBI-356462,EBI-960409From a different organism.

Protein-protein interaction databases

BioGridi248364. 8 interactions.
DIPiDIP-37260N.
IntActiP62260. 9 interactions.
MINTiMINT-1597410.
STRINGi10116.ENSRNOP00000007100.

Structurei

3D structure databases

ProteinModelPortaliP62260.
SMRiP62260. Positions 3-232.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.

Phylogenomic databases

eggNOGiCOG5040.
GeneTreeiENSGT00730000110613.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP62260.
KOiK06630.
OMAiMQESDKP.
OrthoDBiEOG7HHWT3.
PhylomeDBiP62260.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62260-1 [UniParc]FASTAAdd to Basket

« Hide

MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK    50
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI 100
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV 150
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD 200
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV 250
EDENQ 255
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731K → T in AAC52676. 1 Publication
Sequence conflicti120 – 1201F → S in AAC52676. 1 Publication
Sequence conflicti123 – 1231K → Y in AAC52676. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84416 mRNA. Translation: AAC37659.1.
D30739 mRNA. Translation: BAA06401.1.
U53882 mRNA. Translation: AAC52676.1.
BC063163 mRNA. Translation: AAH63163.1.
PIRiJX0341.
RefSeqiNP_113791.1. NM_031603.1.
UniGeneiRn.4225.

Genome annotation databases

EnsembliENSRNOT00000007100; ENSRNOP00000007100; ENSRNOG00000005290.
GeneIDi29753.
KEGGirno:29753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84416 mRNA. Translation: AAC37659.1 .
D30739 mRNA. Translation: BAA06401.1 .
U53882 mRNA. Translation: AAC52676.1 .
BC063163 mRNA. Translation: AAH63163.1 .
PIRi JX0341.
RefSeqi NP_113791.1. NM_031603.1.
UniGenei Rn.4225.

3D structure databases

ProteinModelPortali P62260.
SMRi P62260. Positions 3-232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248364. 8 interactions.
DIPi DIP-37260N.
IntActi P62260. 9 interactions.
MINTi MINT-1597410.
STRINGi 10116.ENSRNOP00000007100.

PTM databases

PhosphoSitei P62260.

2D gel databases

World-2DPAGE 0004:P62260.

Proteomic databases

PaxDbi P62260.
PRIDEi P62260.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007100 ; ENSRNOP00000007100 ; ENSRNOG00000005290 .
GeneIDi 29753.
KEGGi rno:29753.

Organism-specific databases

CTDi 7531.
RGDi 62000. Ywhae.

Phylogenomic databases

eggNOGi COG5040.
GeneTreei ENSGT00730000110613.
HOGENOMi HOG000240379.
HOVERGENi HBG050423.
InParanoidi P62260.
KOi K06630.
OMAi MQESDKP.
OrthoDBi EOG7HHWT3.
PhylomeDBi P62260.
TreeFami TF102003.

Enzyme and pathway databases

Reactomei REACT_198294. Regulation of PLK1 Activity at G2/M Transition.
REACT_198809. Signaling by Hippo.
REACT_199176. Translocation of GLUT4 to the plasma membrane.
REACT_205802. HSF1 activation.
REACT_216489. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

NextBioi 610291.
PROi P62260.

Gene expression databases

Genevestigatori P62260.

Family and domain databases

Gene3Di 1.20.190.20. 1 hit.
InterProi IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view ]
PANTHERi PTHR18860. PTHR18860. 1 hit.
Pfami PF00244. 14-3-3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000868. 14-3-3. 1 hit.
PRINTSi PR00305. 1433ZETA.
SMARTi SM00101. 14_3_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48445. SSF48445. 1 hit.
PROSITEi PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the epsilon and zeta isoforms of the 14-3-3 proteins."
    Roseboom P.H., Weller J.L., Babila T., Aitken A., Sellers L.A., Moffet J.R., Namboodiri M.A., Klein D.C.
    DNA Cell Biol. 13:629-640(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Pineal gland.
  2. "cDNA cloning and characterization of mitochondrial import stimulation factor (MSF) purified from rat liver cytosol."
    Alam R., Hachiya N., Sakaguchi M., Shun-Ichiro K., Iwanaga S., Kitajima M., Mihara K., Omura T.
    J. Biochem. 116:416-425(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Association of a 14-3-3 protein with CMP-NeuAc:GM1 alpha 2,3-sialyltransferase."
    Gao L., Gu X.B., Yu D.S., Yu R.K., Zeng G.
    Biochem. Biophys. Res. Commun. 224:103-107(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  5. Bienvenut W.V., von Kriegsheim A., Kolch W.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-12; 30-42; 95-106; 143-153 AND 216-225, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fibroblast.
  6. Lubec G., Diao W., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 20-50; 62-73; 87-94; 107-123; 131-170 AND 197-225, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  7. "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
    Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C.
    Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 131-142 AND 154-170, INTERACTION WITH AANAT, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry namei1433E_RAT
AccessioniPrimary (citable) accession number: P62260
Secondary accession number(s): P29360, P42655, Q63631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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