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P62259 (1433E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
14-3-3 protein epsilon

Short name=14-3-3E
Gene names
Name:Ywhae
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit structure

Homodimer. Heterodimerizes with YWHAZ By similarity. Interacts with NDEL1, ARHGEF28 and TIAM2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2 By similarity. Interacts with PKA-phosphorylated AANAT By similarity. Interacts with phosphorylated GRB10. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity. Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasm By similarity. Melanosome By similarity.

Developmental stage

In the E8.5 embryo, expressed throughout the embryo. Within a day, expression was more marked in mesenchyme than elsewhere (e.g. epithelial tissue, where it was generally low), although levels in neural tissue rose again by about E12.5. This difference was maintained until E15.5 when expression levels started to drop in most tissues, with those of the nervous system, tooth, and kidney being exceptions. Strongly expressed in early mesenchyme. The expression decreased as the mesenchyme differentiated. Ref.1

Sequence similarities

Belongs to the 14-3-3 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcerebral cortex development

Inferred from mutant phenotype Ref.7. Source: MGI

hippocampus development

Inferred from mutant phenotype Ref.7. Source: MGI

negative regulation of peptidyl-serine dephosphorylation

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein dephosphorylation

Inferred from direct assay Ref.7. Source: MGI

neuron migration

Inferred from mutant phenotype Ref.7. Source: MGI

protein targeting

Inferred from direct assay PubMed 12446771. Source: MGI

regulation of membrane repolarization

Inferred from electronic annotation. Source: Ensembl

regulation of potassium ion transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

cytosol

Traceable author statement. Source: Reactome

extracellular vesicular exosome

Inferred from electronic annotation. Source: Ensembl

kinesin complex

Inferred from electronic annotation. Source: Ensembl

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853. Source: MGI

   Molecular_functionpotassium channel regulator activity

Inferred from electronic annotation. Source: Ensembl

protein domain specific binding

Inferred from direct assay PubMed 12446771. Source: MGI

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 25525514-3-3 protein epsilon
PRO_0000058619

Sites

Site571Interaction with phosphoserine on interacting protein By similarity
Site1301Interaction with phosphoserine on interacting protein By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue501N6-acetyllysine By similarity
Modified residue691N6-acetyllysine By similarity
Modified residue1181N6-acetyllysine By similarity
Modified residue1231N6-acetyllysine By similarity
Modified residue2101Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P62259 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 07817CCBD1F75B26

FASTA25529,174
        10         20         30         40         50         60 
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW 

        70         80         90        100        110        120 
RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF 

       130        140        150        160        170        180 
YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF 

       190        200        210        220        230        240 
YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE 

       250 
EQNKEALQDV EDENQ 

« Hide

References

« Hide 'large scale' references
[1]"The mouse 14-3-3 epsilon isoform, a kinase regulator whose expression pattern is modulated in mesenchyme and neuronal differentiation."
McConnell J.E., Armstrong J.F., Bard J.B.
Dev. Biol. 169:218-228(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: Swiss.
Tissue: Kidney.
[2]Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
[3]"High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ILS and ISS.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]Lubec G., Klug S., Friebe K., Yang J.W., Zigmond M.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 131-141; 154-170 AND 197-215, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain and Hippocampus.
[6]"Identification of a novel interaction of 14-3-3 with p190RhoGEF."
Zhai J., Lin H., Shamim M., Schlaepfer W.W., Canete-Soler R.
J. Biol. Chem. 276:41318-41324(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARHGEF28.
[7]"14-3-3epsilon is important for neuronal migration by binding to NUDEL: a molecular explanation for Miller-Dieker syndrome."
Toyo-oka K., Shionoya A., Gambello M.J., Cardoso C., Leventer R., Ward H.L., Ayala R., Tsai L.-H., Dobyns W., Ledbetter D., Hirotsune S., Wynshaw-Boris A.
Nat. Genet. 34:274-285(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDEL1.
[8]"Phosphorylation of grb10 regulates its interaction with 14-3-3."
Urschel S., Bassermann F., Bai R.Y., Munch S., Peschel C., Duyster J.
J. Biol. Chem. 280:16987-16993(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GRB10.
[9]"Rho-kinase modulates the function of STEF, a Rac GEF, through its phosphorylation."
Takefuji M., Mori K., Morita Y., Arimura N., Nishimura T., Nakayama M., Hoshino M., Iwamatsu A., Murohara T., Kaibuchi K., Amano M.
Biochem. Biophys. Res. Commun. 355:788-794(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIAM2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z19599 mRNA. Translation: CAA79659.1.
D87663 mRNA. Translation: BAA13424.1.
AF483478 mRNA. Translation: AAL90752.1.
AF483479 mRNA. Translation: AAL90753.1.
BC058686 mRNA. Translation: AAH58686.1.
PIRS31975. I48337.
RefSeqNP_033562.3. NM_009536.4.
UniGeneMm.234700.
Mm.471625.

3D structure databases

ProteinModelPortalP62259.
SMRP62259. Positions 3-232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204619. 267 interactions.
IntActP62259. 4023 interactions.
MINTMINT-209058.

PTM databases

PhosphoSiteP62259.

2D gel databases

REPRODUCTION-2DPAGEP62259.

Proteomic databases

PaxDbP62259.
PRIDEP62259.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000067664; ENSMUSP00000070993; ENSMUSG00000020849.
GeneID22627.
KEGGmmu:22627.
UCSCuc007ket.2. mouse.

Organism-specific databases

CTD7531.
MGIMGI:894689. Ywhae.

Phylogenomic databases

eggNOGCOG5040.
HOGENOMHOG000240379.
HOVERGENHBG050423.
InParanoidP62259.
KOK06630.
OMAMQESDKP.
OrthoDBEOG7HHWT3.
PhylomeDBP62259.
TreeFamTF102003.

Enzyme and pathway databases

ReactomeREACT_147847. Translocation of Glut4 to the Plasma Membrane.

Gene expression databases

ArrayExpressP62259.
BgeeP62259.
GenevestigatorP62259.

Family and domain databases

Gene3D1.20.190.20. 1 hit.
InterProIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERPTHR18860. PTHR18860. 1 hit.
PfamPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFPIRSF000868. 14-3-3. 1 hit.
PRINTSPR00305. 1433ZETA.
SMARTSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMSSF48445. SSF48445. 1 hit.
PROSITEPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSYWHAE. mouse.
NextBio302987.
PROP62259.
SOURCESearch...

Entry information

Entry name1433E_MOUSE
AccessionPrimary (citable) accession number: P62259
Secondary accession number(s): P29360, P42655, Q63631
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot