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P62259

- 1433E_MOUSE

UniProt

P62259 - 1433E_MOUSE

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Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Interaction with phosphoserine on interacting proteinBy similarity
Sitei130 – 1301Interaction with phosphoserine on interacting proteinBy similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: Ensembl
  2. potassium channel regulator activity Source: Ensembl
  3. protein domain specific binding Source: MGI

GO - Biological processi

  1. cerebral cortex development Source: MGI
  2. hippocampus development Source: MGI
  3. negative regulation of peptidyl-serine dephosphorylation Source: Ensembl
  4. negative regulation of protein dephosphorylation Source: MGI
  5. neuron migration Source: MGI
  6. protein targeting Source: MGI
  7. regulation of membrane repolarization Source: Ensembl
  8. regulation of potassium ion transmembrane transporter activity Source: Ensembl
  9. substantia nigra development Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_196537. Signaling by Hippo.
REACT_196635. Regulation of PLK1 Activity at G2/M Transition.
REACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_210754. NADE modulates death signalling.
REACT_222185. Regulation of HSF1-mediated heat shock response.
REACT_225256. HSF1 activation.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Gene namesi
Name:Ywhae
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:894689. Ywhae.

Subcellular locationi

Cytoplasm By similarity. Melanosome By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: Reactome
  2. cytosol Source: Reactome
  3. extracellular vesicular exosome Source: Ensembl
  4. kinesin complex Source: Ensembl
  5. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 25525514-3-3 protein epsilonPRO_0000058619Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei50 – 501N6-acetyllysineBy similarity
Modified residuei69 – 691N6-acetyllysineBy similarity
Modified residuei118 – 1181N6-acetyllysineBy similarity
Modified residuei123 – 1231N6-acetyllysineBy similarity
Modified residuei210 – 2101PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP62259.
PaxDbiP62259.
PRIDEiP62259.

2D gel databases

REPRODUCTION-2DPAGEP62259.

PTM databases

PhosphoSiteiP62259.

Expressioni

Developmental stagei

In the E8.5 embryo, expressed throughout the embryo. Within a day, expression was more marked in mesenchyme than elsewhere (e.g. epithelial tissue, where it was generally low), although levels in neural tissue rose again by about E12.5. This difference was maintained until E15.5 when expression levels started to drop in most tissues, with those of the nervous system, tooth, and kidney being exceptions. Strongly expressed in early mesenchyme. The expression decreased as the mesenchyme differentiated.1 Publication

Gene expression databases

BgeeiP62259.
ExpressionAtlasiP62259. baseline and differential.
GenevestigatoriP62259.

Interactioni

Subunit structurei

Homodimer. Heterodimerizes with YWHAZ By similarity. Interacts with NDEL1, ARHGEF28 and TIAM2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2 By similarity. Interacts with PKA-phosphorylated AANAT By similarity. Interacts with phosphorylated GRB10. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Fbxo4Q8CHQ02EBI-356480,EBI-3895153
Hdac7Q8C2B36EBI-356480,EBI-643830
Hist1h3iP6843311EBI-356480,EBI-1179609From a different organism.
Lrrk2Q5S0063EBI-356480,EBI-2693710
Prdx6O352442EBI-356480,EBI-915490From a different organism.
Tbc1d4Q8BYJ66EBI-356480,EBI-7920707
Tfdp2Q64163-46EBI-356480,EBI-8077763

Protein-protein interaction databases

BioGridi204619. 267 interactions.
IntActiP62259. 4024 interactions.
MINTiMINT-209058.

Structurei

3D structure databases

ProteinModelPortaliP62259.
SMRiP62259. Positions 3-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiCOG5040.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP62259.
KOiK06630.
OMAiMQESDKP.
OrthoDBiEOG7HHWT3.
PhylomeDBiP62259.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62259-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
110 120 130 140 150
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
160 170 180 190 200
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV

EDENQ
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19599 mRNA. Translation: CAA79659.1.
D87663 mRNA. Translation: BAA13424.1.
AF483478 mRNA. Translation: AAL90752.1.
AF483479 mRNA. Translation: AAL90753.1.
BC058686 mRNA. Translation: AAH58686.1.
CCDSiCCDS25056.1.
PIRiI48337. S31975.
RefSeqiNP_033562.3. NM_009536.4.
UniGeneiMm.234700.
Mm.471625.

Genome annotation databases

EnsembliENSMUST00000067664; ENSMUSP00000070993; ENSMUSG00000020849.
GeneIDi22627.
KEGGimmu:22627.
UCSCiuc007ket.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z19599 mRNA. Translation: CAA79659.1 .
D87663 mRNA. Translation: BAA13424.1 .
AF483478 mRNA. Translation: AAL90752.1 .
AF483479 mRNA. Translation: AAL90753.1 .
BC058686 mRNA. Translation: AAH58686.1 .
CCDSi CCDS25056.1.
PIRi I48337. S31975.
RefSeqi NP_033562.3. NM_009536.4.
UniGenei Mm.234700.
Mm.471625.

3D structure databases

ProteinModelPortali P62259.
SMRi P62259. Positions 3-232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204619. 267 interactions.
IntActi P62259. 4024 interactions.
MINTi MINT-209058.

PTM databases

PhosphoSitei P62259.

2D gel databases

REPRODUCTION-2DPAGE P62259.

Proteomic databases

MaxQBi P62259.
PaxDbi P62259.
PRIDEi P62259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000067664 ; ENSMUSP00000070993 ; ENSMUSG00000020849 .
GeneIDi 22627.
KEGGi mmu:22627.
UCSCi uc007ket.2. mouse.

Organism-specific databases

CTDi 7531.
MGIi MGI:894689. Ywhae.

Phylogenomic databases

eggNOGi COG5040.
HOGENOMi HOG000240379.
HOVERGENi HBG050423.
InParanoidi P62259.
KOi K06630.
OMAi MQESDKP.
OrthoDBi EOG7HHWT3.
PhylomeDBi P62259.
TreeFami TF102003.

Enzyme and pathway databases

Reactomei REACT_196537. Signaling by Hippo.
REACT_196635. Regulation of PLK1 Activity at G2/M Transition.
REACT_199054. Translocation of GLUT4 to the plasma membrane.
REACT_210754. NADE modulates death signalling.
REACT_222185. Regulation of HSF1-mediated heat shock response.
REACT_225256. HSF1 activation.

Miscellaneous databases

ChiTaRSi YWHAE. mouse.
NextBioi 302987.
PROi P62259.
SOURCEi Search...

Gene expression databases

Bgeei P62259.
ExpressionAtlasi P62259. baseline and differential.
Genevestigatori P62259.

Family and domain databases

Gene3Di 1.20.190.20. 1 hit.
InterProi IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view ]
PANTHERi PTHR18860. PTHR18860. 1 hit.
Pfami PF00244. 14-3-3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000868. 14-3-3. 1 hit.
PRINTSi PR00305. 1433ZETA.
SMARTi SM00101. 14_3_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48445. SSF48445. 1 hit.
PROSITEi PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse 14-3-3 epsilon isoform, a kinase regulator whose expression pattern is modulated in mesenchyme and neuronal differentiation."
    McConnell J.E., Armstrong J.F., Bard J.B.
    Dev. Biol. 169:218-228(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: Swiss.
    Tissue: Kidney.
  2. Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
    Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
    Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ILS and ISS.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  5. Lubec G., Klug S., Friebe K., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 131-141; 154-170 AND 197-215, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Brain and Hippocampus.
  6. "Identification of a novel interaction of 14-3-3 with p190RhoGEF."
    Zhai J., Lin H., Shamim M., Schlaepfer W.W., Canete-Soler R.
    J. Biol. Chem. 276:41318-41324(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARHGEF28.
  7. "14-3-3epsilon is important for neuronal migration by binding to NUDEL: a molecular explanation for Miller-Dieker syndrome."
    Toyo-oka K., Shionoya A., Gambello M.J., Cardoso C., Leventer R., Ward H.L., Ayala R., Tsai L.-H., Dobyns W., Ledbetter D., Hirotsune S., Wynshaw-Boris A.
    Nat. Genet. 34:274-285(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDEL1.
  8. "Phosphorylation of grb10 regulates its interaction with 14-3-3."
    Urschel S., Bassermann F., Bai R.Y., Munch S., Peschel C., Duyster J.
    J. Biol. Chem. 280:16987-16993(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRB10.
  9. Cited for: INTERACTION WITH TIAM2.

Entry informationi

Entry namei1433E_MOUSE
AccessioniPrimary (citable) accession number: P62259
Secondary accession number(s): P29360, P42655, Q63631
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3