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P62259

- 1433E_MOUSE

UniProt

P62259 - 1433E_MOUSE

Protein

14-3-3 protein epsilon

Gene

Ywhae

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei57 – 571Interaction with phosphoserine on interacting proteinBy similarity
    Sitei130 – 1301Interaction with phosphoserine on interacting proteinBy similarity

    GO - Molecular functioni

    1. potassium channel regulator activity Source: Ensembl
    2. protein binding Source: UniProtKB
    3. protein domain specific binding Source: MGI

    GO - Biological processi

    1. cerebral cortex development Source: MGI
    2. hippocampus development Source: MGI
    3. negative regulation of peptidyl-serine dephosphorylation Source: Ensembl
    4. negative regulation of protein dephosphorylation Source: MGI
    5. neuron migration Source: MGI
    6. protein targeting Source: MGI
    7. regulation of membrane repolarization Source: Ensembl
    8. regulation of potassium ion transmembrane transporter activity Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_196537. Signaling by Hippo.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_210754. NADE modulates death signalling.
    REACT_222185. Regulation of HSF1-mediated heat shock response.
    REACT_225256. HSF1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    14-3-3 protein epsilon
    Short name:
    14-3-3E
    Gene namesi
    Name:Ywhae
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:894689. Ywhae.

    Subcellular locationi

    Cytoplasm By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: Reactome
    2. cytosol Source: Reactome
    3. extracellular vesicular exosome Source: Ensembl
    4. kinesin complex Source: Ensembl
    5. melanosome Source: UniProtKB-SubCell
    6. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 25525514-3-3 protein epsilonPRO_0000058619Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei69 – 691N6-acetyllysineBy similarity
    Modified residuei118 – 1181N6-acetyllysineBy similarity
    Modified residuei123 – 1231N6-acetyllysineBy similarity
    Modified residuei210 – 2101PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP62259.
    PaxDbiP62259.
    PRIDEiP62259.

    2D gel databases

    REPRODUCTION-2DPAGEP62259.

    PTM databases

    PhosphoSiteiP62259.

    Expressioni

    Developmental stagei

    In the E8.5 embryo, expressed throughout the embryo. Within a day, expression was more marked in mesenchyme than elsewhere (e.g. epithelial tissue, where it was generally low), although levels in neural tissue rose again by about E12.5. This difference was maintained until E15.5 when expression levels started to drop in most tissues, with those of the nervous system, tooth, and kidney being exceptions. Strongly expressed in early mesenchyme. The expression decreased as the mesenchyme differentiated.1 Publication

    Gene expression databases

    ArrayExpressiP62259.
    BgeeiP62259.
    GenevestigatoriP62259.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimerizes with YWHAZ By similarity. Interacts with NDEL1, ARHGEF28 and TIAM2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2 By similarity. Interacts with PKA-phosphorylated AANAT By similarity. Interacts with phosphorylated GRB10. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Fbxo4Q8CHQ02EBI-356480,EBI-3895153
    Hdac7Q8C2B36EBI-356480,EBI-643830
    Hist1h3iP6843311EBI-356480,EBI-1179609From a different organism.
    Lrrk2Q5S0063EBI-356480,EBI-2693710
    Prdx6O352442EBI-356480,EBI-915490From a different organism.
    Tbc1d4Q8BYJ66EBI-356480,EBI-7920707
    Tfdp2Q64163-46EBI-356480,EBI-8077763

    Protein-protein interaction databases

    BioGridi204619. 267 interactions.
    IntActiP62259. 4024 interactions.
    MINTiMINT-209058.

    Structurei

    3D structure databases

    ProteinModelPortaliP62259.
    SMRiP62259. Positions 3-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 14-3-3 family.Curated

    Phylogenomic databases

    eggNOGiCOG5040.
    HOGENOMiHOG000240379.
    HOVERGENiHBG050423.
    InParanoidiP62259.
    KOiK06630.
    OMAiMQESDKP.
    OrthoDBiEOG7HHWT3.
    PhylomeDBiP62259.
    TreeFamiTF102003.

    Family and domain databases

    Gene3Di1.20.190.20. 1 hit.
    InterProiIPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view]
    PANTHERiPTHR18860. PTHR18860. 1 hit.
    PfamiPF00244. 14-3-3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000868. 14-3-3. 1 hit.
    PRINTSiPR00305. 1433ZETA.
    SMARTiSM00101. 14_3_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48445. SSF48445. 1 hit.
    PROSITEiPS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62259-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK    50
    NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI 100
    LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV 150
    AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD 200
    DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV 250
    EDENQ 255
    Length:255
    Mass (Da):29,174
    Last modified:July 5, 2004 - v1
    Checksum:i07817CCBD1F75B26
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19599 mRNA. Translation: CAA79659.1.
    D87663 mRNA. Translation: BAA13424.1.
    AF483478 mRNA. Translation: AAL90752.1.
    AF483479 mRNA. Translation: AAL90753.1.
    BC058686 mRNA. Translation: AAH58686.1.
    CCDSiCCDS25056.1.
    PIRiI48337. S31975.
    RefSeqiNP_033562.3. NM_009536.4.
    UniGeneiMm.234700.
    Mm.471625.

    Genome annotation databases

    EnsembliENSMUST00000067664; ENSMUSP00000070993; ENSMUSG00000020849.
    GeneIDi22627.
    KEGGimmu:22627.
    UCSCiuc007ket.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19599 mRNA. Translation: CAA79659.1 .
    D87663 mRNA. Translation: BAA13424.1 .
    AF483478 mRNA. Translation: AAL90752.1 .
    AF483479 mRNA. Translation: AAL90753.1 .
    BC058686 mRNA. Translation: AAH58686.1 .
    CCDSi CCDS25056.1.
    PIRi I48337. S31975.
    RefSeqi NP_033562.3. NM_009536.4.
    UniGenei Mm.234700.
    Mm.471625.

    3D structure databases

    ProteinModelPortali P62259.
    SMRi P62259. Positions 3-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204619. 267 interactions.
    IntActi P62259. 4024 interactions.
    MINTi MINT-209058.

    PTM databases

    PhosphoSitei P62259.

    2D gel databases

    REPRODUCTION-2DPAGE P62259.

    Proteomic databases

    MaxQBi P62259.
    PaxDbi P62259.
    PRIDEi P62259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000067664 ; ENSMUSP00000070993 ; ENSMUSG00000020849 .
    GeneIDi 22627.
    KEGGi mmu:22627.
    UCSCi uc007ket.2. mouse.

    Organism-specific databases

    CTDi 7531.
    MGIi MGI:894689. Ywhae.

    Phylogenomic databases

    eggNOGi COG5040.
    HOGENOMi HOG000240379.
    HOVERGENi HBG050423.
    InParanoidi P62259.
    KOi K06630.
    OMAi MQESDKP.
    OrthoDBi EOG7HHWT3.
    PhylomeDBi P62259.
    TreeFami TF102003.

    Enzyme and pathway databases

    Reactomei REACT_196537. Signaling by Hippo.
    REACT_196635. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199054. Translocation of GLUT4 to the plasma membrane.
    REACT_210754. NADE modulates death signalling.
    REACT_222185. Regulation of HSF1-mediated heat shock response.
    REACT_225256. HSF1 activation.

    Miscellaneous databases

    ChiTaRSi YWHAE. mouse.
    NextBioi 302987.
    PROi P62259.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62259.
    Bgeei P62259.
    Genevestigatori P62259.

    Family and domain databases

    Gene3Di 1.20.190.20. 1 hit.
    InterProi IPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view ]
    PANTHERi PTHR18860. PTHR18860. 1 hit.
    Pfami PF00244. 14-3-3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000868. 14-3-3. 1 hit.
    PRINTSi PR00305. 1433ZETA.
    SMARTi SM00101. 14_3_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48445. SSF48445. 1 hit.
    PROSITEi PS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse 14-3-3 epsilon isoform, a kinase regulator whose expression pattern is modulated in mesenchyme and neuronal differentiation."
      McConnell J.E., Armstrong J.F., Bard J.B.
      Dev. Biol. 169:218-228(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
      Strain: Swiss.
      Tissue: Kidney.
    2. Takihara Y., Irie K., Nomura M., Motaleb M., Matsumoto K., Shimada K.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129/Sv.
    3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
      Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
      Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ILS and ISS.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. Lubec G., Klug S., Friebe K., Yang J.W., Zigmond M.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 131-141; 154-170 AND 197-215, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Brain and Hippocampus.
    6. "Identification of a novel interaction of 14-3-3 with p190RhoGEF."
      Zhai J., Lin H., Shamim M., Schlaepfer W.W., Canete-Soler R.
      J. Biol. Chem. 276:41318-41324(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARHGEF28.
    7. "14-3-3epsilon is important for neuronal migration by binding to NUDEL: a molecular explanation for Miller-Dieker syndrome."
      Toyo-oka K., Shionoya A., Gambello M.J., Cardoso C., Leventer R., Ward H.L., Ayala R., Tsai L.-H., Dobyns W., Ledbetter D., Hirotsune S., Wynshaw-Boris A.
      Nat. Genet. 34:274-285(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NDEL1.
    8. "Phosphorylation of grb10 regulates its interaction with 14-3-3."
      Urschel S., Bassermann F., Bai R.Y., Munch S., Peschel C., Duyster J.
      J. Biol. Chem. 280:16987-16993(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRB10.
    9. Cited for: INTERACTION WITH TIAM2.

    Entry informationi

    Entry namei1433E_MOUSE
    AccessioniPrimary (citable) accession number: P62259
    Secondary accession number(s): P29360, P42655, Q63631
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3