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Reviewed, UniProtKB/Swiss-Prot P62258 (1433E_HUMAN)

Last modified November 25, 2008. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    14-3-3 protein epsilon
      Short name=14-3-3E
Gene names
Name: YWHAE
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit structure

Homodimer. Interacts with NDEL1, RGNEF and TIAM2 By similarity. Interacts with HCV core protein.

Subcellular location

CytoplasmBy similarity. Melanosome. Note= Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence similarities

Belongs to the 14-3-3 family.

Ontologies

Keywords

   Biological processHost-virus interaction
   Cellular componentCytoplasm
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Direct protein sequencing

Gene Ontology (GO)

   Biological processinterspecies interaction between organisms

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular signaling cascade Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentmelanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionenzyme binding

Inferred from physical interaction. Source: UniProtKB

protein domain specific binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 25525514-3-3 protein epsilon
PRO_0000058618

Amino acid modifications

Modified residue11N-acetylmethionine
Modified residue2101Phosphoserine

Experimental info

Sequence conflict106 – 1072KH → NY AA sequence Ref.11
Sequence conflict1431E → F AA sequence Ref.11
Sequence conflict1481S → T AA sequence Ref.11

Secondary structure

....................... 255
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P62258-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 07817CCBD1F75B26

FASTA25529,174
        10         20         30         40         50         60 
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW 

        70         80         90        100        110        120 
RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF 

       130        140        150        160        170        180 
YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF 

       190        200        210        220        230        240 
YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE 

       250 
EQNKEALQDV EDENQ 

« Hide

References

« Hide 'large scale' references
[1]"14-3-3 proteins associate with cdc25 phosphatases."
Conklin D.S., Galaktionov K., Beach D.
Proc. Natl. Acad. Sci. U.S.A. 92:7892-7896(1995) [PubMed: 7644510] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"14-3-3 epsilon has no homology to LIS1 and lies telomeric to it on chromosome 17p13.3 outside the Miller-Dieker syndrome chromosome region."
Chong S.S., Tanigami A., Roschke A.V., Ledbetter D.H.
Genome Res. 6:735-741(1996) [PubMed: 8858348] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"Function of 14-3-3 proteins."
Jin D.-Y., Lyu M.S., Kozak C.A., Jeang K.-T.
Nature 382:308-308(1996) [PubMed: 8684458] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Luk S.C.W., Lee C.Y., Waye M.M.Y.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Heart.
[5]"14-3-3 epsilon genomic sequence."
Tanigami A., Chong S.S., Ledbetter D.H.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[9]"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-19.
Tissue: Platelet.
[10]Bienvenut W.V.
Submitted (MAY-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 1-19; 30-50 AND 131-170, ACETYLATION AT MET-1, MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[11]"Isolation and partial characterization of the structures of fibroblast activating factor-related proteins from U937 cells."
Demeter J., Medzihradszky D., Kha H., Goetzl E.J., Turck C.W.
Immunology 72:350-354(1991) [PubMed: 2026444] [Abstract]
Cited for: PROTEIN SEQUENCE OF 103-108; 120-123; 131-141 AND 143-153.
Tissue: Histiocytic lymphoma.
[12]Lubec G., Afjehi-Sadat L.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 131-141 AND 154-190, MASS SPECTROMETRY.
Tissue: Brain and Cajal-Retzius cell.
[13]"Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1."
Aoki H., Hayashi J., Moriyama M., Arakawa Y., Hino O.
J. Virol. 74:1736-1741(2000) [PubMed: 10644344] [Abstract]
Cited for: INTERACTION WITH HCV CORE PROTEIN.
[14]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

U20972 mRNA. Translation: AAC50175.1.
U54778 mRNA. Translation: AAC50710.1.
U43399 mRNA. Translation: AAC50625.1.
U43430 mRNA. Translation: AAD00026.1.
U28936 mRNA. Translation: AAA75301.1.
AB017103 Genomic DNA. Translation: BAA32538.1.
BT007161 mRNA. Translation: AAP35825.1.
CH471108 Genomic DNA. Translation: EAW90628.1.
BC000179 mRNA. Translation: AAH00179.1.
BC001440 mRNA. Translation: AAH01440.1.
PIRA61235.
I38947.
RefSeqNP_006752.1.
UniGeneHs.513851

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2BR9X-ray1.75A1-233[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP62258.

2-D gel databases

OGPP42655.

Proteomic databases

PeptideAtlasP62258.

Genome annotation databases

EnsemblENSG00000108953. Homo sapiens. [Contig view]
GeneID7531.
KEGGhsa:7531.

Organism-specific databases

H-InvDBHIX0013397.
HIX0013751.
HIX0030006.
HGNCHGNC:12851. YWHAE.
HPACAB016200.
HPA008445.
MIM605066. gene.
PharmGKBPA37440.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP62258.
HOVERGENP62258.

Gene expression databases

ArrayExpressP62258.
CleanExHS_YWHAE.
GermOnlineENSG00000108953. Homo sapiens.

Family and domain databases

InterProIPR000308. 14-3-3.
[Graphical view]
Gene3DG3DSA:1.20.190.20. 14-3-3. 1 hit.
PANTHERPTHR18860. 14-3-3. 1 hit.
PfamPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFPIRSF000868. 14-3-3. 1 hit.
PRINTSPR00305. 1433ZETA.
ProDomPD000600. 14-3-3. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00101. 14_3_3. 1 hit.
[Graphical view]
PROSITEPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP62258.
NextBio29461.
SOURCESearch...

Entry information

Entry name1433E_HUMAN
AccessionPrimary (citable) accession number: P62258
Secondary accession number(s): P29360 expand/collapse secondary AC list , P42655, Q53XZ5, Q63631, Q7M4R4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents