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Protein

14-3-3 protein epsilon

Gene

YWHAE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • enzyme binding Source: UniProtKB
  • histone deacetylase binding Source: BHF-UCL
  • ion channel binding Source: BHF-UCL
  • MHC class II protein complex binding Source: UniProtKB
  • phosphoprotein binding Source: BHF-UCL
  • phosphoserine binding Source: BHF-UCL
  • poly(A) RNA binding Source: UniProtKB
  • potassium channel regulator activity Source: BHF-UCL
  • protein heterodimerization activity Source: BHF-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108953-MONOMER.
ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-2028269. Signaling by Hippo.
R-HSA-205025. NADE modulates death signalling.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-3371511. HSF1 activation.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
R-HSA-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-HSA-8854518. AURKA Activation by TPX2.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.
SignaLinkiP62258.
SIGNORiP62258.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Short name:
14-3-3E
Gene namesi
Name:YWHAE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:12851. YWHAE.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • kinesin complex Source: Ensembl
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi7531.
MalaCardsiYWHAE.
OpenTargetsiENSG00000108953.
ENSG00000274474.
Orphaneti217385. 17p13.3 microduplication syndrome.
261257. Distal 17p13.3 microdeletion syndrome.
531. Miller-Dieker syndrome.
PharmGKBiPA37440.

Chemistry databases

ChEMBLiCHEMBL3329082.

Polymorphism and mutation databases

BioMutaiYWHAE.
DMDMi51702210.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586181 – 25514-3-3 protein epsilonAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei50N6-acetyllysineCombined sources1
Modified residuei65PhosphoserineBy similarity1
Modified residuei69N6-acetyllysineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei123N6-acetyllysineCombined sources1
Modified residuei131PhosphotyrosineBy similarity1
Modified residuei137PhosphothreonineBy similarity1
Modified residuei210PhosphoserineCombined sources1
Modified residuei232PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP62258.
PaxDbiP62258.
PeptideAtlasiP62258.
PRIDEiP62258.
TopDownProteomicsiP62258-1. [P62258-1]

2D gel databases

OGPiP42655.
UCD-2DPAGEP62258.

PTM databases

iPTMnetiP62258.
PhosphoSitePlusiP62258.
SwissPalmiP62258.

Miscellaneous databases

PMAP-CutDBP62258.

Expressioni

Gene expression databases

BgeeiENSG00000108953.
CleanExiHS_YWHAE.
ExpressionAtlasiP62258. baseline and differential.
GenevisibleiP62258. HS.

Organism-specific databases

HPAiCAB016200.
CAB021109.
CAB047350.
HPA008445.

Interactioni

Subunit structurei

Homodimer (PubMed:17085597). Heterodimerizes with YWHAZ (PubMed:16376338). Interacts with PKA-phosphorylated AANAT (PubMed:11427721). Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm (PubMed:15696159). Interacts with ARHGEF28 (By similarity). Interacts with BEX3 (By similarity). Weakly interacts with CDKN1B (PubMed:12042314). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with DENND1A (PubMed:26055712). Interacts with GAB2 (PubMed:19172738). Interacts with phosphorylated GRB10 (PubMed:15722337). Interacts with KSR1 (PubMed:10409742). Interacts with NDEL1 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552). Interacts with the phosphorylated (by AKT1) form of SRPK2 (PubMed:19592491). Interacts with TIAM2. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts with ZFP36 (via phosphorylated form) (By similarity).By similarity12 Publications
(Microbial infection) Interacts with HCV core protein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting protein1
Sitei130Interaction with phosphoserine on interacting protein1

Binary interactionsi

WithEntry#Exp.IntActNotes
O929725EBI-356498,EBI-9213553From a different organism.
ACDQ96AP02EBI-356498,EBI-717666
APAF1O147272EBI-356498,EBI-446492
ARAFP103983EBI-356498,EBI-365961
CBX4O00257-32EBI-356498,EBI-4392727
CBY1Q9Y3M23EBI-356498,EBI-947308
CDK14O949213EBI-356498,EBI-1043945
FBXO4Q9UKT55EBI-356498,EBI-960409
HDAC4P565244EBI-356498,EBI-308629
KANK1Q14678-23EBI-356498,EBI-6173812
LRRK2Q5S0076EBI-356498,EBI-5323863
MAP3K3Q997593EBI-356498,EBI-307281
MDM4O151513EBI-356498,EBI-398437
MLF1P583403EBI-356498,EBI-721328
Prdx6O352442EBI-356498,EBI-915490From a different organism.
RAF1P040493EBI-356498,EBI-365996
Rnd3P615882EBI-356498,EBI-6930266From a different organism.
STACQ994692EBI-356498,EBI-2652799
WWTR1Q9GZV53EBI-356498,EBI-747743
YWHABP319463EBI-356498,EBI-359815
YWHAGP619814EBI-356498,EBI-359832
YWHAZP631046EBI-356498,EBI-347088

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • enzyme binding Source: UniProtKB
  • histone deacetylase binding Source: BHF-UCL
  • ion channel binding Source: BHF-UCL
  • MHC class II protein complex binding Source: UniProtKB
  • phosphoprotein binding Source: BHF-UCL
  • phosphoserine binding Source: BHF-UCL
  • protein heterodimerization activity Source: BHF-UCL
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi113363. 369 interactors.
DIPiDIP-36676N.
IntActiP62258. 174 interactors.
MINTiMINT-4998623.
STRINGi9606.ENSP00000264335.

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 17Combined sources14
Helixi20 – 31Combined sources12
Helixi39 – 73Combined sources35
Helixi76 – 106Combined sources31
Helixi108 – 111Combined sources4
Helixi115 – 135Combined sources21
Helixi138 – 162Combined sources25
Helixi168 – 183Combined sources16
Helixi188 – 204Combined sources17
Helixi205 – 208Combined sources4
Turni211 – 213Combined sources3
Helixi214 – 231Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BR9X-ray1.75A1-233[»]
3UALX-ray1.80A1-232[»]
3UBWX-ray1.90A1-234[»]
ProteinModelPortaliP62258.
SMRiP62258.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62258.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP62258.
KOiK06630.
OMAiKESALIM.
OrthoDBiEOG091G0VKY.
PhylomeDBiP62258.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62258-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI
110 120 130 140 150
LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV
160 170 180 190 200
AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV

EDENQ
Length:255
Mass (Da):29,174
Last modified:July 5, 2004 - v1
Checksum:i07817CCBD1F75B26
GO
Isoform SV (identifier: P62258-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Note: Unable to dimerize with YWHAZ.
Show »
Length:233
Mass (Da):26,504
Checksum:i20302F1E0AB73C8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106 – 107KH → NY AA sequence (PubMed:2026444).Curated2
Sequence conflicti143E → F AA sequence (PubMed:2026444).Curated1
Sequence conflicti148S → T AA sequence (PubMed:2026444).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0406211 – 22Missing in isoform SV. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20972 mRNA. Translation: AAC50175.1.
U54778 mRNA. Translation: AAC50710.1.
U43399 mRNA. Translation: AAC50625.1.
U43430 mRNA. Translation: AAD00026.1.
U28936 mRNA. Translation: AAA75301.1.
AB017103 Genomic DNA. Translation: BAA32538.1.
AY883089 mRNA. Translation: AAX68683.1.
AK128785 mRNA. Translation: BAG54733.1.
AK295260 mRNA. Translation: BAG58249.1.
AK316185 mRNA. Translation: BAH14556.1.
BT007161 mRNA. Translation: AAP35825.1.
CH471108 Genomic DNA. Translation: EAW90628.1.
CH471108 Genomic DNA. Translation: EAW90629.1.
BC000179 mRNA. Translation: AAH00179.1.
BC001440 mRNA. Translation: AAH01440.1.
CCDSiCCDS11001.1. [P62258-1]
PIRiA61235.
I38947.
RefSeqiNP_006752.1. NM_006761.4. [P62258-1]
UniGeneiHs.513851.

Genome annotation databases

EnsembliENST00000264335; ENSP00000264335; ENSG00000108953. [P62258-1]
ENST00000571732; ENSP00000461762; ENSG00000108953. [P62258-2]
ENST00000616643; ENSP00000481059; ENSG00000274474. [P62258-2]
ENST00000627231; ENSP00000487356; ENSG00000274474. [P62258-1]
GeneIDi7531.
KEGGihsa:7531.
UCSCiuc002fsk.4. human. [P62258-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20972 mRNA. Translation: AAC50175.1.
U54778 mRNA. Translation: AAC50710.1.
U43399 mRNA. Translation: AAC50625.1.
U43430 mRNA. Translation: AAD00026.1.
U28936 mRNA. Translation: AAA75301.1.
AB017103 Genomic DNA. Translation: BAA32538.1.
AY883089 mRNA. Translation: AAX68683.1.
AK128785 mRNA. Translation: BAG54733.1.
AK295260 mRNA. Translation: BAG58249.1.
AK316185 mRNA. Translation: BAH14556.1.
BT007161 mRNA. Translation: AAP35825.1.
CH471108 Genomic DNA. Translation: EAW90628.1.
CH471108 Genomic DNA. Translation: EAW90629.1.
BC000179 mRNA. Translation: AAH00179.1.
BC001440 mRNA. Translation: AAH01440.1.
CCDSiCCDS11001.1. [P62258-1]
PIRiA61235.
I38947.
RefSeqiNP_006752.1. NM_006761.4. [P62258-1]
UniGeneiHs.513851.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BR9X-ray1.75A1-233[»]
3UALX-ray1.80A1-232[»]
3UBWX-ray1.90A1-234[»]
ProteinModelPortaliP62258.
SMRiP62258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113363. 369 interactors.
DIPiDIP-36676N.
IntActiP62258. 174 interactors.
MINTiMINT-4998623.
STRINGi9606.ENSP00000264335.

Chemistry databases

ChEMBLiCHEMBL3329082.

PTM databases

iPTMnetiP62258.
PhosphoSitePlusiP62258.
SwissPalmiP62258.

Polymorphism and mutation databases

BioMutaiYWHAE.
DMDMi51702210.

2D gel databases

OGPiP42655.
UCD-2DPAGEP62258.

Proteomic databases

EPDiP62258.
PaxDbiP62258.
PeptideAtlasiP62258.
PRIDEiP62258.
TopDownProteomicsiP62258-1. [P62258-1]

Protocols and materials databases

DNASUi7531.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264335; ENSP00000264335; ENSG00000108953. [P62258-1]
ENST00000571732; ENSP00000461762; ENSG00000108953. [P62258-2]
ENST00000616643; ENSP00000481059; ENSG00000274474. [P62258-2]
ENST00000627231; ENSP00000487356; ENSG00000274474. [P62258-1]
GeneIDi7531.
KEGGihsa:7531.
UCSCiuc002fsk.4. human. [P62258-1]

Organism-specific databases

CTDi7531.
DisGeNETi7531.
GeneCardsiYWHAE.
H-InvDBHIX0013751.
HIX0030006.
HGNCiHGNC:12851. YWHAE.
HPAiCAB016200.
CAB021109.
CAB047350.
HPA008445.
MalaCardsiYWHAE.
MIMi605066. gene.
neXtProtiNX_P62258.
OpenTargetsiENSG00000108953.
ENSG00000274474.
Orphaneti217385. 17p13.3 microduplication syndrome.
261257. Distal 17p13.3 microdeletion syndrome.
531. Miller-Dieker syndrome.
PharmGKBiPA37440.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP62258.
KOiK06630.
OMAiKESALIM.
OrthoDBiEOG091G0VKY.
PhylomeDBiP62258.
TreeFamiTF102003.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108953-MONOMER.
ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-2028269. Signaling by Hippo.
R-HSA-205025. NADE modulates death signalling.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-3371511. HSF1 activation.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
R-HSA-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-HSA-8854518. AURKA Activation by TPX2.
R-HSA-8876198. RAB GEFs exchange GTP for GDP on RABs.
SignaLinkiP62258.
SIGNORiP62258.

Miscellaneous databases

ChiTaRSiYWHAE. human.
EvolutionaryTraceiP62258.
GeneWikiiYWHAE.
GenomeRNAii7531.
PMAP-CutDBP62258.
PROiP62258.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108953.
CleanExiHS_YWHAE.
ExpressionAtlasiP62258. baseline and differential.
GenevisibleiP62258. HS.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433E_HUMAN
AccessioniPrimary (citable) accession number: P62258
Secondary accession number(s): B3KY71
, D3DTH5, P29360, P42655, Q4VJB6, Q53XZ5, Q63631, Q7M4R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.