Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 H

Gene

Ube2h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A (By similarity).By similarity

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 H (EC:6.3.2.19)
Alternative name(s):
UBCH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H
Gene namesi
Name:Ube2h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:104632. Ube2h.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Ubiquitin-conjugating enzyme E2 HPRO_0000082487Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP62257.
PaxDbiP62257.
PRIDEiP62257.

PTM databases

PhosphoSiteiP62257.

Expressioni

Gene expression databases

BgeeiP62257.
CleanExiMM_UBE2H.
ExpressionAtlasiP62257. baseline and differential.
GenevisibleiP62257. MM.

Interactioni

Protein-protein interaction databases

BioGridi204417. 1 interaction.
STRINGi10090.ENSMUSP00000100058.

Structurei

3D structure databases

ProteinModelPortaliP62257.
SMRiP62257. Positions 4-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
HOVERGENiHBG063308.
InParanoidiP62257.
OMAiEFCVKFY.
OrthoDBiEOG7Z3F66.
PhylomeDBiP62257.
TreeFamiTF101121.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Length:183
Mass (Da):20,655
Last modified:July 5, 2004 - v1
Checksum:i3DD5D365945F708C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19854 mRNA. Translation: AAA91975.1.
BC008517 mRNA. Translation: AAH08517.1.
CCDSiCCDS19969.1.
PIRiJC4308.
RefSeqiNP_033485.1. NM_009459.3.
UniGeneiMm.332967.
Mm.391754.

Genome annotation databases

EnsembliENSMUST00000102993; ENSMUSP00000100058; ENSMUSG00000039159.
GeneIDi22214.
UCSCiuc009bey.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19854 mRNA. Translation: AAA91975.1.
BC008517 mRNA. Translation: AAH08517.1.
CCDSiCCDS19969.1.
PIRiJC4308.
RefSeqiNP_033485.1. NM_009459.3.
UniGeneiMm.332967.
Mm.391754.

3D structure databases

ProteinModelPortaliP62257.
SMRiP62257. Positions 4-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204417. 1 interaction.
STRINGi10090.ENSMUSP00000100058.

PTM databases

PhosphoSiteiP62257.

Proteomic databases

MaxQBiP62257.
PaxDbiP62257.
PRIDEiP62257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102993; ENSMUSP00000100058; ENSMUSG00000039159.
GeneIDi22214.
UCSCiuc009bey.3. mouse.

Organism-specific databases

CTDi7328.
MGIiMGI:104632. Ube2h.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
HOVERGENiHBG063308.
InParanoidiP62257.
OMAiEFCVKFY.
OrthoDBiEOG7Z3F66.
PhylomeDBiP62257.
TreeFamiTF101121.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi302213.
PROiP62257.
SOURCEiSearch...

Gene expression databases

BgeeiP62257.
CleanExiMM_UBE2H.
ExpressionAtlasiP62257. baseline and differential.
GenevisibleiP62257. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation."
    Wefes I., Mastrandrea L.D., Haldeman M., Koury S.T., Tamburlin J., Pickart C.M., Finley D.
    Proc. Natl. Acad. Sci. U.S.A. 92:4982-4986(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Characterization of a cDNA clone encoding E2-20K, a murine ubiquitin-conjugating enzyme."
    Wefes I., Kaiser P., Schneider R., Pickart C.M., Finley D.
    Gene 163:321-322(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-60, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiUBE2H_MOUSE
AccessioniPrimary (citable) accession number: P62257
Secondary accession number(s): P37286
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.