P62257 (UBE2H_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 H
EC=6.3.2.19

Alternative name(s):
UBCH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H

Gene names

Name:

Ube2h

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Ubiquitin-conjugating enzyme E2 H		PRO_0000082487
Sites
	Active site	87	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62257 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3DD5D365945F708C	FASTA	183	20,655
	10 20 30 40 50 60 MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV WKVRVDLPDK 70 80 90 100 110 120 YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD LTNIFESFLP QLLAYPNPID 130 140 150 160 170 180 PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Induction of ubiquitin-conjugating enzymes during terminal erythroid differentiation." Wefes I., Mastrandrea L.D., Haldeman M., Koury S.T., Tamburlin J., Pickart C.M., Finley D. Proc. Natl. Acad. Sci. U.S.A. 92:4982-4986(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[2]	"Characterization of a cDNA clone encoding E2-20K, a murine ubiquitin-conjugating enzyme." Wefes I., Kaiser P., Schneider R., Pickart C.M., Finley D. Gene 163:321-322(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19854 mRNA. Translation: AAA91975.1. BC008517 mRNA. Translation: AAH08517.1.
IPI	IPI00125481.
PIR	JC4308.
RefSeq	NP_033485.1. NM_009459.3.
UniGene	Mm.332967. Mm.391754.
3D structure databases
ProteinModelPortal	P62257.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000100058.
PTM databases
PhosphoSite	P62257.
Proteomic databases
PaxDb	P62257.
PRIDE	P62257.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102993; ENSMUSP00000100058; ENSMUSG00000039159.
GeneID	22214.
KEGG	mmu:22214.
Organism-specific databases
CTD	7328.
MGI	MGI:104632. Ube2h.
Phylogenomic databases
eggNOG	COG5078.
HOGENOM	HOG000233452.
HOVERGEN	HBG063308.
InParanoid	P62257.
KO	K10576.
OMA	DTDVIKL.
OrthoDB	EOG4D52ZV.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P62257.
Bgee	P62257.
CleanEx	MM_UBE2H.
Genevestigator	P62257.
GermOnline	ENSMUSG00000070636. Mus musculus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	28676.
SOURCE	Search...

Entry information

Entry name

UBE2H_MOUSE

Accession

Primary (citable) accession number: P62257
Secondary accession number(s): P37286

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program