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P62256

- UBE2H_HUMAN

UniProt

P62256 - UBE2H_HUMAN

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Protein

Ubiquitin-conjugating enzyme E2 H

Gene

UBE2H

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein K11-linked ubiquitination Source: UniProtKB
  2. protein K48-linked ubiquitination Source: UniProtKB
  3. ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP62256.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 H (EC:6.3.2.19)
Alternative name(s):
UbcH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H
Gene namesi
Name:UBE2H
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:12484. UBE2H.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37133.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Ubiquitin-conjugating enzyme E2 HPRO_0000082486Add
BLAST

Proteomic databases

MaxQBiP62256.
PaxDbiP62256.
PRIDEiP62256.

PTM databases

PhosphoSiteiP62256.

Expressioni

Gene expression databases

BgeeiP62256.
CleanExiHS_UBE2H.
ExpressionAtlasiP62256. baseline and differential.
GenevestigatoriP62256.

Organism-specific databases

HPAiHPA003302.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MAGEA2BP433562EBI-2129909,EBI-5650739
MAGEC2Q9UBF13EBI-2129909,EBI-5651487

Protein-protein interaction databases

BioGridi113176. 79 interactions.
IntActiP62256. 43 interactions.
STRINGi9606.ENSP00000347836.

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2015Combined sources
Beta strandi21 – 233Combined sources
Beta strandi25 – 306Combined sources
Beta strandi33 – 397Combined sources
Turni45 – 484Combined sources
Beta strandi50 – 567Combined sources
Turni59 – 635Combined sources
Beta strandi67 – 726Combined sources
Turni81 – 833Combined sources
Helixi88 – 947Combined sources
Helixi103 – 1064Combined sources
Helixi108 – 1147Combined sources
Helixi124 – 1329Combined sources
Helixi134 – 14815Combined sources
Helixi151 – 1544Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z5DX-ray2.10A/B1-160[»]
ProteinModelPortaliP62256.
SMRiP62256. Positions 4-155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62256.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
HOVERGENiHBG063308.
InParanoidiP62256.
KOiK10576.
OMAiCIESKHE.
OrthoDBiEOG7Z3F66.
PhylomeDBiP62256.
TreeFamiTF101121.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P62256-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Length:183
Mass (Da):20,655
Last modified:July 5, 2004 - v1
Checksum:i3DD5D365945F708C
GO
Isoform 2 (identifier: P62256-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-99: Missing.

Note: No experimental confirmation available.

Show »
Length:152
Mass (Da):17,174
Checksum:iC24F6341C98931F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti165 – 1651S → P in AAP57630. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei69 – 9931Missing in isoform 2. 1 PublicationVSP_044580Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29328 mRNA. Translation: CAA82525.1.
Z29330 mRNA. Translation: CAA82527.1.
Z29331 mRNA. Translation: CAA82528.1.
AY302138 mRNA. Translation: AAP57630.1.
BT006756 mRNA. Translation: AAP35402.1.
AC073320 Genomic DNA. No translation available.
AC084865 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24099.1.
CH471070 Genomic DNA. Translation: EAW83736.1.
BC006277 mRNA. Translation: AAH06277.1.
CCDSiCCDS47710.1. [P62256-2]
CCDS5814.1. [P62256-1]
PIRiA53516.
RefSeqiNP_001189427.1. NM_001202498.1.
NP_003335.1. NM_003344.3. [P62256-1]
NP_874356.1. NM_182697.2. [P62256-2]
UniGeneiHs.643548.

Genome annotation databases

EnsembliENST00000355621; ENSP00000347836; ENSG00000186591. [P62256-1]
ENST00000473814; ENSP00000419097; ENSG00000186591. [P62256-2]
GeneIDi7328.
KEGGihsa:7328.
UCSCiuc003vpf.2. human. [P62256-1]
uc003vpg.2. human.

Polymorphism databases

DMDMi51338683.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29328 mRNA. Translation: CAA82525.1 .
Z29330 mRNA. Translation: CAA82527.1 .
Z29331 mRNA. Translation: CAA82528.1 .
AY302138 mRNA. Translation: AAP57630.1 .
BT006756 mRNA. Translation: AAP35402.1 .
AC073320 Genomic DNA. No translation available.
AC084865 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24099.1 .
CH471070 Genomic DNA. Translation: EAW83736.1 .
BC006277 mRNA. Translation: AAH06277.1 .
CCDSi CCDS47710.1. [P62256-2 ]
CCDS5814.1. [P62256-1 ]
PIRi A53516.
RefSeqi NP_001189427.1. NM_001202498.1.
NP_003335.1. NM_003344.3. [P62256-1 ]
NP_874356.1. NM_182697.2. [P62256-2 ]
UniGenei Hs.643548.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2Z5D X-ray 2.10 A/B 1-160 [» ]
ProteinModelPortali P62256.
SMRi P62256. Positions 4-155.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113176. 79 interactions.
IntActi P62256. 43 interactions.
STRINGi 9606.ENSP00000347836.

PTM databases

PhosphoSitei P62256.

Polymorphism databases

DMDMi 51338683.

Proteomic databases

MaxQBi P62256.
PaxDbi P62256.
PRIDEi P62256.

Protocols and materials databases

DNASUi 7328.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000355621 ; ENSP00000347836 ; ENSG00000186591 . [P62256-1 ]
ENST00000473814 ; ENSP00000419097 ; ENSG00000186591 . [P62256-2 ]
GeneIDi 7328.
KEGGi hsa:7328.
UCSCi uc003vpf.2. human. [P62256-1 ]
uc003vpg.2. human.

Organism-specific databases

CTDi 7328.
GeneCardsi GC07M129470.
H-InvDB HIX0123428.
HGNCi HGNC:12484. UBE2H.
HPAi HPA003302.
MIMi 601082. gene.
neXtProti NX_P62256.
PharmGKBi PA37133.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00390000004852.
HOGENOMi HOG000233452.
HOVERGENi HBG063308.
InParanoidi P62256.
KOi K10576.
OMAi CIESKHE.
OrthoDBi EOG7Z3F66.
PhylomeDBi P62256.
TreeFami TF101121.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinki P62256.

Miscellaneous databases

ChiTaRSi UBE2H. human.
EvolutionaryTracei P62256.
GeneWikii UBE2H.
GenomeRNAii 7328.
NextBioi 28676.
PROi P62256.
SOURCEi Search...

Gene expression databases

Bgeei P62256.
CleanExi HS_UBE2H.
ExpressionAtlasi P62256. baseline and differential.
Genevestigatori P62256.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
    Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
    J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. Lin L., Li S., Li H., Zhou G., Shen C., Zheng G., Ke R., Zhong G., Yu R., Yang S.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  8. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBE2H_HUMAN
AccessioniPrimary (citable) accession number: P62256
Secondary accession number(s): A4D1L6
, C9JY93, P37286, Q7Z6F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3