Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P62256

- UBE2H_HUMAN

UniProt

P62256 - UBE2H_HUMAN

Protein

Ubiquitin-conjugating enzyme E2 H

Gene

UBE2H

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.2 Publications

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei87 – 871Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein K11-linked ubiquitination Source: UniProtKB
    2. protein K48-linked ubiquitination Source: UniProtKB
    3. ubiquitin-dependent protein catabolic process Source: ProtInc

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinkiP62256.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 H (EC:6.3.2.19)
    Alternative name(s):
    UbcH2
    Ubiquitin carrier protein H
    Ubiquitin-conjugating enzyme E2-20K
    Ubiquitin-protein ligase H
    Gene namesi
    Name:UBE2H
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:12484. UBE2H.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37133.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Ubiquitin-conjugating enzyme E2 HPRO_0000082486Add
    BLAST

    Proteomic databases

    MaxQBiP62256.
    PaxDbiP62256.
    PRIDEiP62256.

    PTM databases

    PhosphoSiteiP62256.

    Expressioni

    Gene expression databases

    ArrayExpressiP62256.
    BgeeiP62256.
    CleanExiHS_UBE2H.
    GenevestigatoriP62256.

    Organism-specific databases

    HPAiHPA003302.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MAGEA2BP433562EBI-2129909,EBI-5650739
    MAGEC2Q9UBF13EBI-2129909,EBI-5651487

    Protein-protein interaction databases

    BioGridi113176. 78 interactions.
    IntActiP62256. 43 interactions.
    STRINGi9606.ENSP00000347836.

    Structurei

    Secondary structure

    1
    183
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 2015
    Beta strandi21 – 233
    Beta strandi25 – 306
    Beta strandi33 – 397
    Turni45 – 484
    Beta strandi50 – 567
    Turni59 – 635
    Beta strandi67 – 726
    Turni81 – 833
    Helixi88 – 947
    Helixi103 – 1064
    Helixi108 – 1147
    Helixi124 – 1329
    Helixi134 – 14815
    Helixi151 – 1544

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Z5DX-ray2.10A/B1-160[»]
    ProteinModelPortaliP62256.
    SMRiP62256. Positions 4-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP62256.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233452.
    HOVERGENiHBG063308.
    InParanoidiP62256.
    KOiK10576.
    OMAiCIESKHE.
    OrthoDBiEOG7Z3F66.
    PhylomeDBiP62256.
    TreeFamiTF101121.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P62256-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV    50
    WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD 100
    LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT 150
    EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL 183
    Length:183
    Mass (Da):20,655
    Last modified:July 5, 2004 - v1
    Checksum:i3DD5D365945F708C
    GO
    Isoform 2 (identifier: P62256-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         69-99: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:152
    Mass (Da):17,174
    Checksum:iC24F6341C98931F0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti165 – 1651S → P in AAP57630. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei69 – 9931Missing in isoform 2. 1 PublicationVSP_044580Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29328 mRNA. Translation: CAA82525.1.
    Z29330 mRNA. Translation: CAA82527.1.
    Z29331 mRNA. Translation: CAA82528.1.
    AY302138 mRNA. Translation: AAP57630.1.
    BT006756 mRNA. Translation: AAP35402.1.
    AC073320 Genomic DNA. No translation available.
    AC084865 Genomic DNA. No translation available.
    CH236950 Genomic DNA. Translation: EAL24099.1.
    CH471070 Genomic DNA. Translation: EAW83736.1.
    BC006277 mRNA. Translation: AAH06277.1.
    CCDSiCCDS47710.1. [P62256-2]
    CCDS5814.1. [P62256-1]
    PIRiA53516.
    RefSeqiNP_001189427.1. NM_001202498.1.
    NP_003335.1. NM_003344.3. [P62256-1]
    NP_874356.1. NM_182697.2. [P62256-2]
    UniGeneiHs.643548.

    Genome annotation databases

    EnsembliENST00000355621; ENSP00000347836; ENSG00000186591. [P62256-1]
    ENST00000473814; ENSP00000419097; ENSG00000186591. [P62256-2]
    GeneIDi7328.
    KEGGihsa:7328.
    UCSCiuc003vpf.2. human. [P62256-1]
    uc003vpg.2. human.

    Polymorphism databases

    DMDMi51338683.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29328 mRNA. Translation: CAA82525.1 .
    Z29330 mRNA. Translation: CAA82527.1 .
    Z29331 mRNA. Translation: CAA82528.1 .
    AY302138 mRNA. Translation: AAP57630.1 .
    BT006756 mRNA. Translation: AAP35402.1 .
    AC073320 Genomic DNA. No translation available.
    AC084865 Genomic DNA. No translation available.
    CH236950 Genomic DNA. Translation: EAL24099.1 .
    CH471070 Genomic DNA. Translation: EAW83736.1 .
    BC006277 mRNA. Translation: AAH06277.1 .
    CCDSi CCDS47710.1. [P62256-2 ]
    CCDS5814.1. [P62256-1 ]
    PIRi A53516.
    RefSeqi NP_001189427.1. NM_001202498.1.
    NP_003335.1. NM_003344.3. [P62256-1 ]
    NP_874356.1. NM_182697.2. [P62256-2 ]
    UniGenei Hs.643548.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Z5D X-ray 2.10 A/B 1-160 [» ]
    ProteinModelPortali P62256.
    SMRi P62256. Positions 4-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113176. 78 interactions.
    IntActi P62256. 43 interactions.
    STRINGi 9606.ENSP00000347836.

    PTM databases

    PhosphoSitei P62256.

    Polymorphism databases

    DMDMi 51338683.

    Proteomic databases

    MaxQBi P62256.
    PaxDbi P62256.
    PRIDEi P62256.

    Protocols and materials databases

    DNASUi 7328.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000355621 ; ENSP00000347836 ; ENSG00000186591 . [P62256-1 ]
    ENST00000473814 ; ENSP00000419097 ; ENSG00000186591 . [P62256-2 ]
    GeneIDi 7328.
    KEGGi hsa:7328.
    UCSCi uc003vpf.2. human. [P62256-1 ]
    uc003vpg.2. human.

    Organism-specific databases

    CTDi 7328.
    GeneCardsi GC07M129470.
    H-InvDB HIX0123428.
    HGNCi HGNC:12484. UBE2H.
    HPAi HPA003302.
    MIMi 601082. gene.
    neXtProti NX_P62256.
    PharmGKBi PA37133.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233452.
    HOVERGENi HBG063308.
    InParanoidi P62256.
    KOi K10576.
    OMAi CIESKHE.
    OrthoDBi EOG7Z3F66.
    PhylomeDBi P62256.
    TreeFami TF101121.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    SignaLinki P62256.

    Miscellaneous databases

    ChiTaRSi UBE2H. human.
    EvolutionaryTracei P62256.
    GeneWikii UBE2H.
    GenomeRNAii 7328.
    NextBioi 28676.
    PROi P62256.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62256.
    Bgeei P62256.
    CleanExi HS_UBE2H.
    Genevestigatori P62256.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A human ubiquitin-conjugating enzyme homologous to yeast UBC8."
      Kaiser P., Seufert W., Hoefferer L., Kofler B., Sachsenmaier C., Herzog H., Jentsch S., Schweiger M., Schneider R.
      J. Biol. Chem. 269:8797-8802(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    2. Lin L., Li S., Li H., Zhou G., Shen C., Zheng G., Ke R., Zhong G., Yu R., Yang S.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Human chromosome 7: DNA sequence and biology."
      Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
      , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
      Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    8. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
      David Y., Ziv T., Admon A., Navon A.
      J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBE2H_HUMAN
    AccessioniPrimary (citable) accession number: P62256
    Secondary accession number(s): A4D1L6
    , C9JY93, P37286, Q7Z6F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3