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Protein

Ubiquitin-conjugating enzyme E2 H

Gene

UBE2H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: Reactome
  • ubiquitin-dependent protein catabolic process Source: ProtInc

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP62256.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 H (EC:2.3.2.231 Publication)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme H (EC:2.3.2.241 Publication)
E2 ubiquitin-conjugating enzyme H
UbcH2
Ubiquitin carrier protein H
Ubiquitin-conjugating enzyme E2-20K
Ubiquitin-protein ligase H
Gene namesi
Name:UBE2H
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000186591.11.
HGNCiHGNC:12484. UBE2H.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi7328.
OpenTargetsiENSG00000186591.
PharmGKBiPA37133.

Polymorphism and mutation databases

BioMutaiUBE2H.
DMDMi51338683.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824861 – 183Ubiquitin-conjugating enzyme E2 HAdd BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-acetyllysineBy similarity1

Post-translational modificationi

Autoubiquitinated in vitro in the presence of NEDD4L.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiP62256.
MaxQBiP62256.
PaxDbiP62256.
PeptideAtlasiP62256.
PRIDEiP62256.

PTM databases

iPTMnetiP62256.
PhosphoSitePlusiP62256.

Expressioni

Gene expression databases

BgeeiENSG00000186591.
CleanExiHS_UBE2H.
ExpressionAtlasiP62256. baseline and differential.
GenevisibleiP62256. HS.

Organism-specific databases

HPAiHPA003302.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113176. 88 interactors.
IntActiP62256. 50 interactors.
STRINGi9606.ENSP00000347836.

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 20Combined sources15
Beta strandi21 – 23Combined sources3
Beta strandi25 – 30Combined sources6
Beta strandi33 – 39Combined sources7
Turni45 – 48Combined sources4
Beta strandi50 – 56Combined sources7
Turni59 – 63Combined sources5
Beta strandi67 – 72Combined sources6
Turni81 – 83Combined sources3
Helixi88 – 94Combined sources7
Helixi103 – 106Combined sources4
Helixi108 – 114Combined sources7
Helixi124 – 132Combined sources9
Helixi134 – 148Combined sources15
Helixi151 – 154Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Z5DX-ray2.10A/B1-160[»]
ProteinModelPortaliP62256.
SMRiP62256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62256.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0416. Eukaryota.
ENOG410XRC5. LUCA.
GeneTreeiENSGT00390000004852.
HOGENOMiHOG000233452.
HOVERGENiHBG063308.
InParanoidiP62256.
KOiK10576.
OMAiMRHPKEY.
OrthoDBiEOG091G0RI1.
PhylomeDBiP62256.
TreeFamiTF101121.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62256-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSPSPGKRR MDTDVVKLIE SKHEVTILGG LNEFVVKFYG PQGTPYEGGV
60 70 80 90 100
WKVRVDLPDK YPFKSPSIGF MNKIFHPNID EASGTVCLDV INQTWTALYD
110 120 130 140 150
LTNIFESFLP QLLAYPNPID PLNGDAAAMY LHRPEEYKQK IKEYIQKYAT
160 170 180
EEALKEQEEG TGDSSSESSM SDFSEDEAQD MEL
Length:183
Mass (Da):20,655
Last modified:July 5, 2004 - v1
Checksum:i3DD5D365945F708C
GO
Isoform 2 (identifier: P62256-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-99: Missing.

Note: No experimental confirmation available.
Show »
Length:152
Mass (Da):17,174
Checksum:iC24F6341C98931F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165S → P in AAP57630 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04458069 – 99Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29328 mRNA. Translation: CAA82525.1.
Z29330 mRNA. Translation: CAA82527.1.
Z29331 mRNA. Translation: CAA82528.1.
AY302138 mRNA. Translation: AAP57630.1.
BT006756 mRNA. Translation: AAP35402.1.
AC073320 Genomic DNA. No translation available.
AC084865 Genomic DNA. No translation available.
CH236950 Genomic DNA. Translation: EAL24099.1.
CH471070 Genomic DNA. Translation: EAW83736.1.
BC006277 mRNA. Translation: AAH06277.1.
CCDSiCCDS47710.1. [P62256-2]
CCDS5814.1. [P62256-1]
PIRiA53516.
RefSeqiNP_001189427.1. NM_001202498.1.
NP_003335.1. NM_003344.3. [P62256-1]
NP_874356.1. NM_182697.2. [P62256-2]
UniGeneiHs.643548.

Genome annotation databases

EnsembliENST00000355621; ENSP00000347836; ENSG00000186591. [P62256-1]
ENST00000473814; ENSP00000419097; ENSG00000186591. [P62256-2]
GeneIDi7328.
KEGGihsa:7328.
UCSCiuc003vpf.3. human. [P62256-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBE2H_HUMAN
AccessioniPrimary (citable) accession number: P62256
Secondary accession number(s): A4D1L6
, C9JY93, P37286, Q7Z6F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families