Reviewed, UniProtKB/Swiss-Prot P62255 (UB2G1_RAT)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 G1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase G1
    Ubiquitin carrier protein G1
    E217K
    UBC7

Gene names

Name:	Ube2g1
Synonyms:	Ubc7, Ube2g

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of muscle-specific proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Widely expressed, with higher level in testis. Ref.1
Developmental stage	Induced from days 15 to 30.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	protein ubiquitination Ref.1 Traceable author statement. Source: RGD regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.1 Traceable author statement. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 170	169	Ubiquitin-conjugating enzyme E2 G1		PRO_0000082482
Sites
	Active site	90	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62255-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 36B61766D995B332	FASTA	170	19,509
	10 20 30 40 50 60 MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY EGGVFKAHLT 70 80 90 100 110 120 FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED KYGYEKPEER WLPIHTVETI 130 140 150 160 170 MISVISMLAD PNGDSPANVD AAKEWREDRN GEFKRKVARC VRKSQETAFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of rabbit reticulocyte E217K as a UBC7 homologue and functional characterization of its core domain loop." Lin H., Wing S.S. J. Biol. Chem. 274:14685-14691(1999) [PubMed: 10329663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF099093 mRNA. Translation: AAC69605.1. BC086980 mRNA. Translation: AAH86980.1.
IPI	IPI00389665.
RefSeq	NP_073181.1.
UniGene	Rn.163149
3D structure databases
HSSP	HSSP built from PDB template 1PZV based on UniProtKB P34477.
SMR	P62255. Positions 5-165, 6-166.
ModBase	Search...
Protein-protein interaction databases
STRING	P62255.
Genome annotation databases
Ensembl	ENSRNOT00000013486; ENSRNOP00000013486; ENSRNOG00000010041; Rattus norvegicus. [Genome view]
GeneID	64631.
KEGG	rno:64631.
UCSC	NM_022690. rat.
Organism-specific databases
CTD	64631.
RGD	620392. Ube2g1.
Phylogenomic databases
HOVERGEN	P62255.
OMA	DIWHPNV.
Enzyme and pathway databases
BRENDA	6.3.2.19. 248.
Gene expression databases
ArrayExpress	P62255.
Genevestigator	P62255.
GermOnline	ENSRNOG00000010041. Rattus norvegicus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613592.

Entry information

Entry name

UB2G1_RAT

Accession

Primary (citable) accession number: P62255
Secondary accession number(s): Q99462

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)