P62255 (UB2G1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 G1
EC=6.3.2.19

Alternative name(s):
E217K
UBC7
Ubiquitin carrier protein G1
Ubiquitin-protein ligase G1

Gene names

Name:	Ube2g1
Synonyms:	Ubc7, Ube2g

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4 By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Widely expressed, with higher level in testis. Ref.1
Developmental stage	Induced from days 15 to 30.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Traceable author statement Ref.1. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 170	169	Ubiquitin-conjugating enzyme E2 G1		PRO_0000082482
Sites
	Active site	90	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62255 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 36B61766D995B332	FASTA	170	19,509
	10 20 30 40 50 60 MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY EGGVFKAHLT 70 80 90 100 110 120 FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED KYGYEKPEER WLPIHTVETI 130 140 150 160 170 MISVISMLAD PNGDSPANVD AAKEWREDRN GEFKRKVARC VRKSQETAFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of rabbit reticulocyte E217K as a UBC7 homologue and functional characterization of its core domain loop." Lin H., Wing S.S. J. Biol. Chem. 274:14685-14691(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF099093 mRNA. Translation: AAC69605.1. BC086980 mRNA. Translation: AAH86980.1.
IPI	IPI00389665.
RefSeq	NP_073181.1. NM_022690.2.
UniGene	Rn.163149.
3D structure databases
ProteinModelPortal	P62255.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000013486.
PTM databases
PhosphoSite	P62255.
Proteomic databases
PaxDb	P62255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013486; ENSRNOP00000013486; ENSRNOG00000010041.
GeneID	64631.
KEGG	rno:64631.
Organism-specific databases
CTD	7326.
RGD	620392. Ube2g1.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00530000063258.
HOGENOM	HOG000233454.
HOVERGEN	HBG063308.
InParanoid	P62255.
KO	K10575.
OMA	EWREDRH.
OrthoDB	EOG4TTGJZ.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P62255.
Genevestigator	P62255.
GermOnline	ENSRNOG00000010041. Rattus norvegicus.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613592.

Entry information

Entry name

UB2G1_RAT

Accession

Primary (citable) accession number: P62255
Secondary accession number(s): Q99462

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program