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P62255 (UB2G1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 G1

EC=6.3.2.19
Alternative name(s):
E217K
UBC7
Ubiquitin carrier protein G1
Ubiquitin-protein ligase G1
Gene names
Name:Ube2g1
Synonyms:Ubc7, Ube2g
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4 By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Tissue specificity

Widely expressed, with higher level in testis. Ref.1

Developmental stage

Induced from days 15 to 30.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 170170Ubiquitin-conjugating enzyme E2 G1
PRO_0000082482
Initiator methionine11Removed; alternate By similarity
Chain2 – 170169Ubiquitin-conjugating enzyme E2 G1, N-terminally processed
PRO_0000424517

Sites

Active site901Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue21N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processed By similarity

Sequences

Sequence LengthMass (Da)Tools
P62255 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 36B61766D995B332

FASTA17019,509
        10         20         30         40         50         60 
MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY EGGVFKAHLT 

        70         80         90        100        110        120 
FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED KYGYEKPEER WLPIHTVETI 

       130        140        150        160        170 
MISVISMLAD PNGDSPANVD AAKEWREDRN GEFKRKVARC VRKSQETAFE 

« Hide

References

« Hide 'large scale' references
[1]"Identification of rabbit reticulocyte E217K as a UBC7 homologue and functional characterization of its core domain loop."
Lin H., Wing S.S.
J. Biol. Chem. 274:14685-14691(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF099093 mRNA. Translation: AAC69605.1.
BC086980 mRNA. Translation: AAH86980.1.
RefSeqNP_073181.1. NM_022690.2.
UniGeneRn.163149.

3D structure databases

ProteinModelPortalP62255.
SMRP62255. Positions 5-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013486.

PTM databases

PhosphoSiteP62255.

Proteomic databases

PaxDbP62255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013486; ENSRNOP00000013486; ENSRNOG00000010041.
GeneID64631.
KEGGrno:64631.

Organism-specific databases

CTD7326.
RGD620392. Ube2g1.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00730000110436.
HOGENOMHOG000233454.
HOVERGENHBG063308.
InParanoidP62255.
KOK10575.
OMAEWREDRH.
OrthoDBEOG7VB2HT.
PhylomeDBP62255.
TreeFamTF101118.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorP62255.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613592.
PROP62255.

Entry information

Entry nameUB2G1_RAT
AccessionPrimary (citable) accession number: P62255
Secondary accession number(s): Q99462
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways