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P62255

- UB2G1_RAT

UniProt

P62255 - UB2G1_RAT

Protein

Ubiquitin-conjugating enzyme E2 G1

Gene

Ube2g1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4 By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. protein K48-linked ubiquitination Source: UniProtKB
    2. protein K63-linked ubiquitination Source: UniProtKB
    3. protein ubiquitination Source: RGD
    4. ubiquitin-dependent protein catabolic process Source: RGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 G1 (EC:6.3.2.19)
    Alternative name(s):
    E217K
    UBC7
    Ubiquitin carrier protein G1
    Ubiquitin-protein ligase G1
    Cleaved into the following chain:
    Gene namesi
    Name:Ube2g1
    Synonyms:Ubc7, Ube2g
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi620392. Ube2g1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 170170Ubiquitin-conjugating enzyme E2 G1PRO_0000082482Add
    BLAST
    Initiator methioninei1 – 11Removed; alternateBy similarity
    Chaini2 – 170169Ubiquitin-conjugating enzyme E2 G1, N-terminally processedPRO_0000424517Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei2 – 21N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processedBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP62255.

    PTM databases

    PhosphoSiteiP62255.

    Expressioni

    Tissue specificityi

    Widely expressed, with higher level in testis.1 Publication

    Developmental stagei

    Induced from days 15 to 30.

    Gene expression databases

    GenevestigatoriP62255.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000013486.

    Structurei

    3D structure databases

    ProteinModelPortaliP62255.
    SMRiP62255. Positions 5-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110436.
    HOGENOMiHOG000233454.
    HOVERGENiHBG063308.
    InParanoidiP62255.
    KOiK10575.
    OMAiEWREDRH.
    OrthoDBiEOG7VB2HT.
    PhylomeDBiP62255.
    TreeFamiTF101118.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P62255-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY    50
    EGGVFKAHLT FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED 100
    KYGYEKPEER WLPIHTVETI MISVISMLAD PNGDSPANVD AAKEWREDRN 150
    GEFKRKVARC VRKSQETAFE 170
    Length:170
    Mass (Da):19,509
    Last modified:January 23, 2007 - v3
    Checksum:i36B61766D995B332
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099093 mRNA. Translation: AAC69605.1.
    BC086980 mRNA. Translation: AAH86980.1.
    RefSeqiNP_073181.1. NM_022690.2.
    UniGeneiRn.163149.

    Genome annotation databases

    EnsembliENSRNOT00000013486; ENSRNOP00000013486; ENSRNOG00000010041.
    GeneIDi64631.
    KEGGirno:64631.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099093 mRNA. Translation: AAC69605.1 .
    BC086980 mRNA. Translation: AAH86980.1 .
    RefSeqi NP_073181.1. NM_022690.2.
    UniGenei Rn.163149.

    3D structure databases

    ProteinModelPortali P62255.
    SMRi P62255. Positions 5-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000013486.

    PTM databases

    PhosphoSitei P62255.

    Proteomic databases

    PaxDbi P62255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013486 ; ENSRNOP00000013486 ; ENSRNOG00000010041 .
    GeneIDi 64631.
    KEGGi rno:64631.

    Organism-specific databases

    CTDi 7326.
    RGDi 620392. Ube2g1.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110436.
    HOGENOMi HOG000233454.
    HOVERGENi HBG063308.
    InParanoidi P62255.
    KOi K10575.
    OMAi EWREDRH.
    OrthoDBi EOG7VB2HT.
    PhylomeDBi P62255.
    TreeFami TF101118.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 613592.
    PROi P62255.

    Gene expression databases

    Genevestigatori P62255.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of rabbit reticulocyte E217K as a UBC7 homologue and functional characterization of its core domain loop."
      Lin H., Wing S.S.
      J. Biol. Chem. 274:14685-14691(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiUB2G1_RAT
    AccessioniPrimary (citable) accession number: P62255
    Secondary accession number(s): Q99462
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3