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Protein

Ubiquitin-conjugating enzyme E2 G1

Gene

Ube2g1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.By similarity

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme G1
E217K
UBC7
Ubiquitin carrier protein G1
Ubiquitin-protein ligase G1
Cleaved into the following chain:
Gene namesi
Name:Ube2g1
Synonyms:Ube2g
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914378. Ube2g1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000824811 – 170Ubiquitin-conjugating enzyme E2 G1Add BLAST170
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004245162 – 170Ubiquitin-conjugating enzyme E2 G1, N-terminally processedAdd BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processedBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP62254.
PaxDbiP62254.
PeptideAtlasiP62254.
PRIDEiP62254.

2D gel databases

REPRODUCTION-2DPAGEIPI00310850.
P62254.

PTM databases

iPTMnetiP62254.
PhosphoSitePlusiP62254.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020794.
ExpressionAtlasiP62254. baseline and differential.
GenevisibleiP62254. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211962. 3 interactors.
IntActiP62254. 1 interactor.
MINTiMINT-2736817.
STRINGi10090.ENSMUSP00000021148.

Structurei

3D structure databases

ProteinModelPortaliP62254.
SMRiP62254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0425. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP62254.
KOiK10575.
OMAiGFFKCHL.
OrthoDBiEOG091G0O9F.
PhylomeDBiP62254.
TreeFamiTF101118.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62254-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY
60 70 80 90 100
EGGVFKAHLT FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED
110 120 130 140 150
KYGYEKPEER WLPIHTVETI MISVISMLAD PNGDSPANVD AAKEWREDRN
160 170
GEFKRKVARC VRKSQETAFE
Length:170
Mass (Da):19,509
Last modified:January 23, 2007 - v3
Checksum:i36B61766D995B332
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013902 mRNA. Translation: BAB29048.1.
BC096474 mRNA. Translation: AAH96474.1.
CCDSiCCDS36217.1.
RefSeqiNP_080261.2. NM_025985.4.
UniGeneiMm.458052.

Genome annotation databases

EnsembliENSMUST00000021148; ENSMUSP00000021148; ENSMUSG00000020794.
GeneIDi67128.
KEGGimmu:67128.
UCSCiuc007jzc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013902 mRNA. Translation: BAB29048.1.
BC096474 mRNA. Translation: AAH96474.1.
CCDSiCCDS36217.1.
RefSeqiNP_080261.2. NM_025985.4.
UniGeneiMm.458052.

3D structure databases

ProteinModelPortaliP62254.
SMRiP62254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211962. 3 interactors.
IntActiP62254. 1 interactor.
MINTiMINT-2736817.
STRINGi10090.ENSMUSP00000021148.

PTM databases

iPTMnetiP62254.
PhosphoSitePlusiP62254.

2D gel databases

REPRODUCTION-2DPAGEIPI00310850.
P62254.

Proteomic databases

EPDiP62254.
PaxDbiP62254.
PeptideAtlasiP62254.
PRIDEiP62254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021148; ENSMUSP00000021148; ENSMUSG00000020794.
GeneIDi67128.
KEGGimmu:67128.
UCSCiuc007jzc.1. mouse.

Organism-specific databases

CTDi7326.
MGIiMGI:1914378. Ube2g1.

Phylogenomic databases

eggNOGiKOG0425. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP62254.
KOiK10575.
OMAiGFFKCHL.
OrthoDBiEOG091G0O9F.
PhylomeDBiP62254.
TreeFamiTF101118.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

ChiTaRSiUbe2g1. mouse.
PROiP62254.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020794.
ExpressionAtlasiP62254. baseline and differential.
GenevisibleiP62254. MM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2G1_MOUSE
AccessioniPrimary (citable) accession number: P62254
Secondary accession number(s): Q4VAA6, Q99462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.