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P62253

- UB2G1_HUMAN

UniProt

P62253 - UB2G1_HUMAN

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Protein
Ubiquitin-conjugating enzyme E2 G1
Gene
UBE2G1, UBE2G
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.2 Publications

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin protein ligase binding Source: UniProtKB
  4. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked ubiquitination Source: UniProtKB
  2. protein K63-linked ubiquitination Source: UniProtKB
  3. ubiquitin-dependent protein catabolic process Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP62253.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G1 (EC:6.3.2.19)
Alternative name(s):
E217K
UBC7
Ubiquitin carrier protein G1
Ubiquitin-protein ligase G1
Cleaved into the following chain:
Gene namesi
Name:UBE2G1
Synonyms:UBE2G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:12482. UBE2G1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37131.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 170170Ubiquitin-conjugating enzyme E2 G1
PRO_0000424514Add
BLAST
Initiator methioninei1 – 11Removed; alternate1 Publication
Chaini2 – 170169Ubiquitin-conjugating enzyme E2 G1, N-terminally processed
PRO_0000082480Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei2 – 21N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processed3 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP62253.
PaxDbiP62253.
PeptideAtlasiP62253.
PRIDEiP62253.

PTM databases

PhosphoSiteiP62253.

Expressioni

Tissue specificityi

Widely expressed, mainly in skeletal muscle.1 Publication

Gene expression databases

ArrayExpressiP62253.
BgeeiP62253.
CleanExiHS_UBE2G1.
GenevestigatoriP62253.

Organism-specific databases

HPAiHPA045681.
HPA050551.

Interactioni

Protein-protein interaction databases

BioGridi113174. 14 interactions.
IntActiP62253. 1 interaction.
STRINGi9606.ENSP00000380178.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1912
Beta strandi25 – 317
Beta strandi37 – 437
Turni49 – 524
Beta strandi54 – 607
Turni63 – 664
Beta strandi71 – 744
Helixi92 – 943
Helixi117 – 12812

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AWFX-ray2.10A8-160[»]
ProteinModelPortaliP62253.
SMRiP62253. Positions 5-166.

Miscellaneous databases

EvolutionaryTraceiP62253.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP62253.
KOiK10575.
OMAiEWREDRH.
OrthoDBiEOG7VB2HT.
PhylomeDBiP62253.
TreeFamiTF101118.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62253-1 [UniParc]FASTAAdd to Basket

« Hide

MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY    50
EGGVFKAHLT FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED 100
KYGYEKPEER WLPIHTVETI MISVISMLAD PNGDSPANVD AAKEWREDRN 150
GEFKRKVARC VRKSQETAFE 170
Length:170
Mass (Da):19,509
Last modified:January 23, 2007 - v3
Checksum:i36B61766D995B332
GO

Sequence cautioni

The sequence AAH26288.2 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78514 mRNA. Translation: BAA11410.1.
BT007416 mRNA. Translation: AAP36084.1.
AK313059 mRNA. Translation: BAG35889.1.
CH471108 Genomic DNA. Translation: EAW90445.1.
CH471108 Genomic DNA. Translation: EAW90446.1.
BC002775 mRNA. Translation: AAH02775.1.
BC026288 mRNA. Translation: AAH26288.2. Different initiation.
CCDSiCCDS32532.1.
RefSeqiNP_003333.1. NM_003342.4.
UniGeneiHs.741319.

Genome annotation databases

EnsembliENST00000396981; ENSP00000380178; ENSG00000132388.
GeneIDi7326.
KEGGihsa:7326.
UCSCiuc002fxs.3. human.

Polymorphism databases

DMDMi51338681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D78514 mRNA. Translation: BAA11410.1 .
BT007416 mRNA. Translation: AAP36084.1 .
AK313059 mRNA. Translation: BAG35889.1 .
CH471108 Genomic DNA. Translation: EAW90445.1 .
CH471108 Genomic DNA. Translation: EAW90446.1 .
BC002775 mRNA. Translation: AAH02775.1 .
BC026288 mRNA. Translation: AAH26288.2 . Different initiation.
CCDSi CCDS32532.1.
RefSeqi NP_003333.1. NM_003342.4.
UniGenei Hs.741319.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AWF X-ray 2.10 A 8-160 [» ]
ProteinModelPortali P62253.
SMRi P62253. Positions 5-166.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113174. 14 interactions.
IntActi P62253. 1 interaction.
STRINGi 9606.ENSP00000380178.

PTM databases

PhosphoSitei P62253.

Polymorphism databases

DMDMi 51338681.

Proteomic databases

MaxQBi P62253.
PaxDbi P62253.
PeptideAtlasi P62253.
PRIDEi P62253.

Protocols and materials databases

DNASUi 7326.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000396981 ; ENSP00000380178 ; ENSG00000132388 .
GeneIDi 7326.
KEGGi hsa:7326.
UCSCi uc002fxs.3. human.

Organism-specific databases

CTDi 7326.
GeneCardsi GC17M004120.
HGNCi HGNC:12482. UBE2G1.
HPAi HPA045681.
HPA050551.
MIMi 601569. gene.
neXtProti NX_P62253.
PharmGKBi PA37131.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233454.
HOVERGENi HBG063308.
InParanoidi P62253.
KOi K10575.
OMAi EWREDRH.
OrthoDBi EOG7VB2HT.
PhylomeDBi P62253.
TreeFami TF101118.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinki P62253.

Miscellaneous databases

ChiTaRSi UBE2G1. human.
EvolutionaryTracei P62253.
GeneWikii UBE2G1.
GenomeRNAii 7326.
NextBioi 28666.
PROi P62253.
SOURCEi Search...

Gene expression databases

ArrayExpressi P62253.
Bgeei P62253.
CleanExi HS_UBE2G1.
Genevestigatori P62253.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of UBE2G, encoding a human skeletal muscle-specific ubiquitin-conjugating enzyme homologous to UBC7 of C. elegans."
    Watanabe T.K., Kawai A., Fujiwara T., Maekawa H., Hirai Y., Nakamura Y., Takahashi E.
    Cytogenet. Cell Genet. 74:146-148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Caudate nucleus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lymph and Prostate.
  6. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
    Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
    Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11, ACETYLATION AT THR-2.
    Tissue: Platelet.
  7. "CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases."
    Pabarcus M.K., Hoe N., Sadeghi S., Patterson C., Wiertz E., Correia M.A.
    Arch. Biochem. Biophys. 483:66-74(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."
    David Y., Ziv T., Admon A., Navon A.
    J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1 AND THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUB2G1_HUMAN
AccessioniPrimary (citable) accession number: P62253
Secondary accession number(s): B2R7P2, D3DTK0, Q99462
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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