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Protein

Ubiquitin-conjugating enzyme E2 G1

Gene

UBE2G1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: ParkinsonsUK-UCL
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular protein catabolic process Source: MGI
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein K63-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: Reactome
  • ubiquitin-dependent protein catabolic process Source: ProtInc

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6. 2681.
ReactomeiR-HSA-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiP62253.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 G1 (EC:2.3.2.231 Publication)
Alternative name(s):
E2 ubiquitin-conjugating enzyme G1
E217K
UBC7
Ubiquitin carrier protein G1
Ubiquitin-protein ligase G1
Cleaved into the following chain:
Gene namesi
Name:UBE2G1
Synonyms:UBE2G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000132388.12.
HGNCiHGNC:12482. UBE2G1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi7326.
OpenTargetsiENSG00000132388.
PharmGKBiPA37131.

Polymorphism and mutation databases

BioMutaiUBE2G1.
DMDMi51338681.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004245141 – 170Ubiquitin-conjugating enzyme E2 G1Add BLAST170
Initiator methionineiRemoved; alternateCombined sources1 Publication
ChainiPRO_00000824802 – 170Ubiquitin-conjugating enzyme E2 G1, N-terminally processedAdd BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylthreonine; in Ubiquitin-conjugating enzyme E2 G1, N-terminally processedCombined sources1 Publication1

Post-translational modificationi

Autoubiquitinated in vitro.1 Publication

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiP62253.
MaxQBiP62253.
PaxDbiP62253.
PeptideAtlasiP62253.
PRIDEiP62253.

PTM databases

iPTMnetiP62253.
PhosphoSitePlusiP62253.
SwissPalmiP62253.

Expressioni

Tissue specificityi

Widely expressed, mainly in skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000132388.
CleanExiHS_UBE2G1.
ExpressionAtlasiP62253. baseline and differential.
GenevisibleiP62253. HS.

Organism-specific databases

HPAiHPA045681.
HPA050551.

Interactioni

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi113174. 24 interactors.
IntActiP62253. 3 interactors.
STRINGi9606.ENSP00000380178.

Structurei

Secondary structure

1170
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 19Combined sources12
Beta strandi25 – 31Combined sources7
Beta strandi37 – 43Combined sources7
Turni49 – 52Combined sources4
Beta strandi54 – 60Combined sources7
Turni63 – 66Combined sources4
Beta strandi71 – 74Combined sources4
Helixi92 – 94Combined sources3
Helixi117 – 128Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AWFX-ray2.10A8-160[»]
ProteinModelPortaliP62253.
SMRiP62253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62253.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0425. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110436.
HOGENOMiHOG000233454.
HOVERGENiHBG063308.
InParanoidiP62253.
KOiK10575.
OMAiFITPMWH.
OrthoDBiEOG091G0O9F.
PhylomeDBiP62253.
TreeFamiTF101118.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTELQSALLL RRQLAELNKN PVEGFSAGLI DDNDLYRWEV LIIGPPDTLY
60 70 80 90 100
EGGVFKAHLT FPKDYPLRPP KMKFITEIWH PNVDKNGDVC ISILHEPGED
110 120 130 140 150
KYGYEKPEER WLPIHTVETI MISVISMLAD PNGDSPANVD AAKEWREDRN
160 170
GEFKRKVARC VRKSQETAFE
Length:170
Mass (Da):19,509
Last modified:January 23, 2007 - v3
Checksum:i36B61766D995B332
GO

Sequence cautioni

The sequence AAH26288 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78514 mRNA. Translation: BAA11410.1.
BT007416 mRNA. Translation: AAP36084.1.
AK313059 mRNA. Translation: BAG35889.1.
CH471108 Genomic DNA. Translation: EAW90445.1.
CH471108 Genomic DNA. Translation: EAW90446.1.
BC002775 mRNA. Translation: AAH02775.1.
BC026288 mRNA. Translation: AAH26288.2. Different initiation.
CCDSiCCDS32532.1.
RefSeqiNP_003333.1. NM_003342.4.
UniGeneiHs.741319.

Genome annotation databases

EnsembliENST00000396981; ENSP00000380178; ENSG00000132388.
GeneIDi7326.
KEGGihsa:7326.
UCSCiuc002fxs.4. human.

Similar proteinsi

Entry informationi

Entry nameiUB2G1_HUMAN
AccessioniPrimary (citable) accession number: P62253
Secondary accession number(s): B2R7P2, D3DTK0, Q99462
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families