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Protein

40S ribosomal protein S8

Gene

RPS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. structural constituent of ribosome Source: GO_Central

GO - Biological processi

  1. cellular protein metabolic process Source: Reactome
  2. gene expression Source: Reactome
  3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  4. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
  5. SRP-dependent cotranslational protein targeting to membrane Source: Reactome
  6. translation Source: UniProtKB
  7. translational elongation Source: Reactome
  8. translational initiation Source: Reactome
  9. translational termination Source: Reactome
  10. viral life cycle Source: Reactome
  11. viral process Source: Reactome
  12. viral transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiREACT_1079. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1979. Translation initiation complex formation.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_931. Ribosomal scanning and start codon recognition.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S8
Gene namesi
Name:RPS8
ORF Names:OK/SW-cl.83
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:10441. RPS8.

Subcellular locationi

Cytoplasm 1 Publication
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. cytosol Source: Reactome
  3. cytosolic small ribosomal subunit Source: UniProtKB
  4. extracellular vesicular exosome Source: UniProtKB
  5. focal adhesion Source: UniProtKB
  6. membrane Source: UniProtKB
  7. nucleus Source: UniProtKB
  8. ribonucleoprotein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34856.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 20820740S ribosomal protein S8PRO_0000122240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371N6-acetyllysineBy similarity
Modified residuei128 – 1281N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP62241.
PaxDbiP62241.
PeptideAtlasiP62241.
PRIDEiP62241.

PTM databases

PhosphoSiteiP62241.

Miscellaneous databases

PMAP-CutDBP62241.

Expressioni

Gene expression databases

BgeeiP62241.
CleanExiHS_RPS8.
ExpressionAtlasiP62241. baseline.
GenevestigatoriP62241.

Organism-specific databases

HPAiHPA029266.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NDRG1Q925971EBI-351811,EBI-716486

Protein-protein interaction databases

BioGridi112116. 248 interactions.
IntActiP62241. 26 interactions.
MINTiMINT-1141293.
STRINGi9606.ENSP00000379888.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00AI1-208[»]
ProteinModelPortaliP62241.
SMRiP62241. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S8e family.Curated

Phylogenomic databases

eggNOGiCOG2007.
HOGENOMiHOG000211544.
HOVERGENiHBG030738.
InParanoidiP62241.
KOiK02995.
OMAiYNATSND.
PhylomeDBiP62241.
TreeFamiTF300041.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. S8e. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG
60 70 80 90 100
GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNEL VRTKTLVKNC
110 120 130 140 150
IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD
160 170 180 190 200
ERKKNAKISS LLEEQFQQGK LLACIASRPG QCGRADGYVL EGKELEFYLR

KIKARKGK
Length:208
Mass (Da):24,205
Last modified:January 23, 2007 - v2
Checksum:i407FDF59171F7422
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti110 – 1101R → G.
Corresponds to variant rs11537870 [ dbSNP | Ensembl ].
VAR_051861

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67247 Genomic DNA. Translation: CAA47670.1.
AB062401 mRNA. Translation: BAB93488.1.
BC070875 mRNA. Translation: AAH70875.1.
CCDSiCCDS513.1.
PIRiS25022.
RefSeqiNP_001003.1. NM_001012.1.
UniGeneiHs.512675.

Genome annotation databases

EnsembliENST00000396651; ENSP00000379888; ENSG00000142937.
GeneIDi6202.
KEGGihsa:6202.
UCSCiuc001cmi.3. human.

Polymorphism databases

DMDMi50403622.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67247 Genomic DNA. Translation: CAA47670.1.
AB062401 mRNA. Translation: BAB93488.1.
BC070875 mRNA. Translation: AAH70875.1.
CCDSiCCDS513.1.
PIRiS25022.
RefSeqiNP_001003.1. NM_001012.1.
UniGeneiHs.512675.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Xelectron microscopy5.00AI1-208[»]
ProteinModelPortaliP62241.
SMRiP62241. Positions 2-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112116. 248 interactions.
IntActiP62241. 26 interactions.
MINTiMINT-1141293.
STRINGi9606.ENSP00000379888.

PTM databases

PhosphoSiteiP62241.

Polymorphism databases

DMDMi50403622.

Proteomic databases

MaxQBiP62241.
PaxDbiP62241.
PeptideAtlasiP62241.
PRIDEiP62241.

Protocols and materials databases

DNASUi6202.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396651; ENSP00000379888; ENSG00000142937.
GeneIDi6202.
KEGGihsa:6202.
UCSCiuc001cmi.3. human.

Organism-specific databases

CTDi6202.
GeneCardsiGC01P045240.
HGNCiHGNC:10441. RPS8.
HPAiHPA029266.
MIMi600357. gene.
neXtProtiNX_P62241.
PharmGKBiPA34856.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2007.
HOGENOMiHOG000211544.
HOVERGENiHBG030738.
InParanoidiP62241.
KOiK02995.
OMAiYNATSND.
PhylomeDBiP62241.
TreeFamiTF300041.

Enzyme and pathway databases

ReactomeiREACT_1079. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_115902. SRP-dependent cotranslational protein targeting to membrane.
REACT_1404. Peptide chain elongation.
REACT_1797. Formation of a pool of free 40S subunits.
REACT_1979. Translation initiation complex formation.
REACT_1986. Eukaryotic Translation Termination.
REACT_2085. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_75768. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_75822. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_79. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_931. Ribosomal scanning and start codon recognition.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

ChiTaRSiRPS8. human.
GeneWikiiRPS8.
GenomeRNAii6202.
NextBioi24087.
PMAP-CutDBP62241.
PROiP62241.
SOURCEiSearch...

Gene expression databases

BgeeiP62241.
CleanExiHS_RPS8.
ExpressionAtlasiP62241. baseline.
GenevestigatoriP62241.

Family and domain databases

InterProiIPR001047. Ribosomal_S8e.
IPR022309. Ribosomal_S8e/biogenesis_NSA2.
IPR018283. Ribosomal_S8e_CS.
[Graphical view]
PANTHERiPTHR10394. PTHR10394. 1 hit.
PfamiPF01201. Ribosomal_S8e. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00307. S8e. 1 hit.
PROSITEiPS01193. RIBOSOMAL_S8E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The structure of the human intron-containing S8 ribosomal protein gene and determination of its chromosomal location at 1p32-p34.1."
    Davies B., Fried M.
    Genomics 15:68-75(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients."
    Shichijo S., Itoh K.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon adenocarcinoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  4. "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry."
    Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K., Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.
    Eur. J. Biochem. 239:144-149(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-17, CLEAVAGE OF INITIATOR METHIONINE.
    Tissue: Placenta.
  5. Cited for: IDENTIFICATION IN A MRNP GRANULE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  11. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).

Entry informationi

Entry nameiRS8_HUMAN
AccessioniPrimary (citable) accession number: P62241
Secondary accession number(s): P09058, Q6IRL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Ribosomal proteins
    Ribosomal proteins families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.