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P62223

- GLND_RHOPA

UniProt

P62223 - GLND_RHOPA

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:RPA0591
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192762Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP62223. 1 interaction.
MINTiMINT-6732844.
STRINGi258594.RPA0591.

Structurei

3D structure databases

ProteinModelPortaliP62223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 59296HDUniRule annotationAdd
BLAST
Domaini736 – 81883ACT 1UniRule annotationAdd
BLAST
Domaini849 – 92981ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseAdd
BLAST
Regioni380 – 735356Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSPSRPAADE RFDSARVAAE IATLAEKHTG NDAAFRTALA MLMKAELAKA
60 70 80 90 100
RTEAEAQLLR DRHGRRCAER LCYVQDAIIR LLFNAATEYL YNTPTPSSSE
110 120 130 140 150
RMTVVATGGY GRGLMAPESD IDLLFILPYK QTAWGEQVAE VILYCLWDIG
160 170 180 190 200
LKVGHATRSV DECIRQARAD MTIRTAILET RFLAGDEALY AELVERFDKE
210 220 230 240 250
VVEGTAAEFV AAKLAEREER HRRSGQSRYL VEPNVKDGKG GLRDLHTLFW
260 270 280 290 300
IAKYVYRVRE ASELSERGVF DPAEFRTFRR CEDFLWSVRC NIHFVTKRAE
310 320 330 340 350
DRLSFDLQRE IGVRLGYTSH PGMQDVERFM KHYFLIAKEV GNLTAILCAK
360 370 380 390 400
LEDQQAKAAP ALTRMMARLR PAAKRRRVPE SDDFVIDNNR INLAVPDVFK
410 420 430 440 450
HDPVNLIRIF RLAQKNNLAF HPDAMRSVTR SLSLITPQLR DNPEANRLFV
460 470 480 490 500
EILTSDNAEP VLRRMNETGV LGRFIRAFGR IVSMMQFNMY HSYTVDEHLI
510 520 530 540 550
RCVGNLQEIE RGGNDEFALS SELIRKIRPD HRAVLYAAVL LHDIAKGQPE
560 570 580 590 600
DHSTAGAKVA RRLCPRFGFS TADTELVAWL IEKHLVMSTV AQSRDLSDRK
610 620 630 640 650
TIENFAAVVE TVEQMKMLTI LTTADIRGVG PGVWNGWKAQ LIRTLYYETE
660 670 680 690 700
PVLTGGFSEV NRAERIRAAQ AEFRAAFTEW PEADLNAYVA RHYPAYWLKV
710 720 730 740 750
DLQRKIRHAR FLRASEQAGH KLAINVGFDE ARAVTELTIL AVDHPWLLSV
760 770 780 790 800
IAGACASAGA NIVDAQIYTT TDGRALDTIS ISREYDRDED EGRRATRIGE
810 820 830 840 850
TIEEVLEGKL RLPEAVARRA SSGSKAKLRA FVVEPEVEIN NNWSDRYTVI
860 870 880 890 900
EVSGLDRPGL LYQLTTAISK LNLNIASAHV ATFGERARDV FYVTDLLGAQ
910 920
ITAPTRQAAI KRALVHLLAN GDAAEKPAA
Length:929
Mass (Da):104,542
Last modified:July 5, 2004 - v1
Checksum:iBC13BFA4845F8785
GO

Sequence cautioni

The sequence CAE26035.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572594 Genomic DNA. Translation: CAE26035.1. Different initiation.
RefSeqiNP_945944.1. NC_005296.1.
WP_011156159.1. NC_005296.1.

Genome annotation databases

EnsemblBacteriaiCAE26035; CAE26035; RPA0591.
GeneIDi2692917.
KEGGirpa:RPA0591.
PATRICi23285359. VBIRhoPal84835_0625.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572594 Genomic DNA. Translation: CAE26035.1 . Different initiation.
RefSeqi NP_945944.1. NC_005296.1.
WP_011156159.1. NC_005296.1.

3D structure databases

ProteinModelPortali P62223.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P62223. 1 interaction.
MINTi MINT-6732844.
STRINGi 258594.RPA0591.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE26035 ; CAE26035 ; RPA0591 .
GeneIDi 2692917.
KEGGi rpa:RPA0591.
PATRICi 23285359. VBIRhoPal84835_0625.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.

Entry informationi

Entry nameiGLND_RHOPA
AccessioniPrimary (citable) accession number: P62223
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3