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Protein

26S protease regulatory subunit 8

Gene

Psmc5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi190 – 1978ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: RGD
  • ATP binding Source: UniProtKB-KW
  • TBP-class protein binding Source: RGD
  • thyrotropin-releasing hormone receptor binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1169091. Activation of NF-kappaB in B cells.
R-RNO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-RNO-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-RNO-195253. Degradation of beta-catenin by the destruction complex.
R-RNO-202424. Downstream TCR signaling.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2871837. FCERI mediated NF-kB activation.
R-RNO-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-RNO-350562. Regulation of ornithine decarboxylase (ODC).
R-RNO-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-RNO-4608870. Asymmetric localization of PCP proteins.
R-RNO-4641257. Degradation of AXIN.
R-RNO-4641258. Degradation of DVL.
R-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-RNO-5607764. CLEC7A (Dectin-1) signaling.
R-RNO-5610780. Degradation of GLI1 by the proteasome.
R-RNO-5610785. GLI3 is processed to GLI3R by the proteasome.
R-RNO-5632684. Hedgehog 'on' state.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5668541. TNFR2 non-canonical NF-kB pathway.
R-RNO-5676590. NIK-->noncanonical NF-kB signaling.
R-RNO-5687128. MAPK6/MAPK4 signaling.
R-RNO-68827. CDT1 association with the CDC6:ORC:origin complex.
R-RNO-68949. Orc1 removal from chromatin.
R-RNO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-RNO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-RNO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 8
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT6
Proteasome 26S subunit ATPase 5
Proteasome subunit p45
Thyroid hormone receptor-interacting protein 1
Short name:
TRIP1
p45/SUG
Gene namesi
Name:Psmc5
Synonyms:Sug1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi708376. Psmc5.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: Ensembl
  • cytoplasm Source: UniProtKB
  • cytoplasmic vesicle Source: Ensembl
  • cytosolic proteasome complex Source: RGD
  • extracellular exosome Source: Ensembl
  • inclusion body Source: RGD
  • membrane Source: Ensembl
  • nuclear proteasome complex Source: RGD
  • nucleus Source: UniProtKB
  • proteasome accessory complex Source: UniProtKB
  • proteasome complex Source: UniProtKB
  • proteasome regulatory particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 40640526S protease regulatory subunit 8PRO_0000084724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei222 – 2221N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP62198.
PRIDEiP62198.

2D gel databases

World-2DPAGE0004:P62198.

PTM databases

iPTMnetiP62198.
PhosphoSiteiP62198.

Expressioni

Gene expression databases

GenevisibleiP62198. RN.

Interactioni

Subunit structurei

Component of a complex with USP49 and RUVBL1. Interacts with PRPF19. Interacts with TRIM5. Interacts with NDC80. Interacts with PAAF1. Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249685. 15 interactions.
IntActiP62198. 3 interactions.
MINTiMINT-4108806.
STRINGi10116.ENSRNOP00000013997.

Structurei

3D structure databases

ProteinModelPortaliP62198.
SMRiP62198. Positions 27-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 406221May mediate interaction with PRPF9By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0728. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074947.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62198.
KOiK03066.
OMAiVMGTKKV.
OrthoDBiEOG7TF78Z.
PhylomeDBiP62198.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN
60 70 80 90 100
ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD
110 120 130 140 150
KNIDINDVTP NCRVALRNDS YTLHKILPNK VDPLVSLMMV EKVPDSTYEM
160 170 180 190 200
IGGLDKQIKE IKEVIELPVK HPELFEALGI AQPKGVLLYG PPGTGKTLLA
210 220 230 240 250
RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE HAPSIIFMDE
260 270 280 290 300
IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI
310 320 330 340 350
LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL
360 370 380 390 400
MPGASGAEVK GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS

IKKLWK
Length:406
Mass (Da):45,626
Last modified:June 21, 2004 - v1
Checksum:i29C6410C4A85A7F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83521 mRNA. Translation: BAA11938.1.
AB000491 mRNA. Translation: BAA22933.1.
AB000493 Genomic DNA. Translation: BAA22935.1.
BC058462 mRNA. Translation: AAH58462.1.
PIRiT43799.
RefSeqiNP_112411.1. NM_031149.1.
UniGeneiRn.10972.

Genome annotation databases

EnsembliENSRNOT00000013997; ENSRNOP00000013997; ENSRNOG00000010038.
GeneIDi81827.
KEGGirno:81827.
UCSCiRGD:708376. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83521 mRNA. Translation: BAA11938.1.
AB000491 mRNA. Translation: BAA22933.1.
AB000493 Genomic DNA. Translation: BAA22935.1.
BC058462 mRNA. Translation: AAH58462.1.
PIRiT43799.
RefSeqiNP_112411.1. NM_031149.1.
UniGeneiRn.10972.

3D structure databases

ProteinModelPortaliP62198.
SMRiP62198. Positions 27-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249685. 15 interactions.
IntActiP62198. 3 interactions.
MINTiMINT-4108806.
STRINGi10116.ENSRNOP00000013997.

PTM databases

iPTMnetiP62198.
PhosphoSiteiP62198.

2D gel databases

World-2DPAGE0004:P62198.

Proteomic databases

PaxDbiP62198.
PRIDEiP62198.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013997; ENSRNOP00000013997; ENSRNOG00000010038.
GeneIDi81827.
KEGGirno:81827.
UCSCiRGD:708376. rat.

Organism-specific databases

CTDi5705.
RGDi708376. Psmc5.

Phylogenomic databases

eggNOGiKOG0728. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074947.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62198.
KOiK03066.
OMAiVMGTKKV.
OrthoDBiEOG7TF78Z.
PhylomeDBiP62198.

Enzyme and pathway databases

ReactomeiR-RNO-1169091. Activation of NF-kappaB in B cells.
R-RNO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-RNO-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-RNO-195253. Degradation of beta-catenin by the destruction complex.
R-RNO-202424. Downstream TCR signaling.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2871837. FCERI mediated NF-kB activation.
R-RNO-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-RNO-350562. Regulation of ornithine decarboxylase (ODC).
R-RNO-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-RNO-4608870. Asymmetric localization of PCP proteins.
R-RNO-4641257. Degradation of AXIN.
R-RNO-4641258. Degradation of DVL.
R-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-RNO-5607764. CLEC7A (Dectin-1) signaling.
R-RNO-5610780. Degradation of GLI1 by the proteasome.
R-RNO-5610785. GLI3 is processed to GLI3R by the proteasome.
R-RNO-5632684. Hedgehog 'on' state.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5668541. TNFR2 non-canonical NF-kB pathway.
R-RNO-5676590. NIK-->noncanonical NF-kB signaling.
R-RNO-5687128. MAPK6/MAPK4 signaling.
R-RNO-68827. CDT1 association with the CDC6:ORC:origin complex.
R-RNO-68949. Orc1 removal from chromatin.
R-RNO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-RNO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-RNO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi615767.
PROiP62198.

Gene expression databases

GenevisibleiP62198. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structures of the rat proteasomal ATPases: determination of highly conserved structural motifs and rules for their spacing."
    Makino Y., Yogosawa S., Kanemaki M., Yoshida T., Yamano K., Kishimoto T., Moncollin V., Egly J.-M., Muramatsu M., Tamura T.
    Biochem. Biophys. Res. Commun. 220:1049-1054(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Gene coding for the transcription factor, SUG/proteasome, p45 is located nearly 40 kb downstream from the rat growth hormone gene."
    Kazahari K., Nomoto K., Nakazato S., Ono M.
    Gene 198:323-327(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Wistar.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  4. "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor."
    Lee J.W., Choi H.-S., Gyuris J., Brent R., Moore D.D.
    Mol. Endocrinol. 9:243-254(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THYROID HORMONE RECEPTOR.

Entry informationi

Entry nameiPRS8_RAT
AccessioniPrimary (citable) accession number: P62198
Secondary accession number(s): O35051
, P47210, P52915, P52916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.